scholarly journals Antibiotic Susceptibility of Bacteria Isolated from Abattoir Effluent-Impacted Tagangu River, Aliero, Kebbi State, North-Western Nigeria

2019 ◽  
pp. 1-8 ◽  
Author(s):  
B. G. Jega ◽  
O. O. Adebisi ◽  
S. S. Manga
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Microbiological Quality ◽  
Most Probable Number ◽  
Resistant Bacteria ◽  
Proteus Vulgaris ◽  
Probable Number ◽  
Antibiotic Susceptibility Test ◽  
The Impact

This study aimed to evaluate the impact of abattoir effluent on microbiological quality of the receiving Tagangu River and the susceptibility of the isolates to commonly-used antibiotics. The most probable number (MPN) as well as the Kirby-Bauer method of antibiotic susceptibility test were used and demonstrated the total heterotrophic bacteria as well as Escherichia coli O157:H7 numbers in a total of 30 water samples collected over a period of three months at three strategic points of the river. In accordance with CLSI guidelines, four out of eight bacteria (Enterobacter sp., Pseudomonas aeruginosa, Proteus vulgaris and Citrobacter sp.) isolated, demonstrated multiple antibiotic resistance (MAR) against at least three out of septrin, chloramphenicol, amoxicillin, augmentin, gentamicin, tarivid and streptomycin. All the isolates (Escherichia coli, Klebsiella pneumoniae, Enterobacter sp., Pseudomonas aeruginosa, Proteus vulgaris, Citrobacter sp., Serratia marcescens and Aerobacter aerogenes) showed either high or intermediate susceptibility to sparfloxacin, ciprofloxacin and pefloxacin. The findings indicated that the river has been heavily polluted with the effluent discharges and did not meet any of the WHO guidelines for natural water sources fit for irrigation or other domestic purposes. As such, indiscriminate discharge of abattoir effluent could impact on the microbiological quality and promote increased incidence of multiple antibiotic resistant bacteria in a receiving river.

scholarly journals PREVALENCE OF RESISTANCE TO CLINICALLY SIGNIFICANT ANTIBIOTICS AGAINST Escherichia coli ISOLATED FROM HOLY RIVER GANGA, INDIA

2018 ◽  
Vol 7 (6) ◽  
Author(s):  
Shiwani Chaudhary ◽  
Dushyant Singh ◽  
Amita Gaurav Dimri ◽  
Aishwarya Pillai ◽  
M.L. Aggarwal
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Antibiotic Susceptibility ◽  
Large Population ◽  
Microbiological Quality ◽  
Diffusion Method ◽  
Most Probable Number ◽  
Probable Number ◽  
River Ganga ◽  
Ganga Water

Ganga is a divine water body which has acquired a status of mother goddess in Indian culture civilization. Ganga water is considered as sacred water and Hindu rituals from birth to death cannot be consecrated without Ganga water. The microbiological quality of holy river Ganga, life sustaining surface water resource for large population of northern India is adversely affected due to rapid industrialization and population growth. The current investigation surveyed on total number of 10 water samples collected from river Ganga flowing in different cities of India. The samples were analyzed for Most Probable Number (MPN) of coliforms and found to be in the range of 940 organisms to 33000 organisms per 100ml. Similarly, MPN Fecal coliform was done at an incubation of higher temperature, and resulted from lower of 109 organisms to a higher at 2800 organisms per 100ml of samples analyzed. The Ganga water samples also showed the presence of Escherichia coli and further its antibiotic susceptibility pattern was performed by Kirby Bauer Agar well diffusion method. Various clinical significant antibiotics were used namely Streptomycin, Kanamycin, Meropenem, Norfloxacin, Ciprofloxacin, Ampicillin, Sulbactum and Tazobactum. All isolated Escherichia coli strains were found resistant to Tazobactum. The isolated E.coli from upstream water of Varanasi has shown resistance towards four out of eight antibiotics used. Escherichia coli present in all the samples have shown susceptibility towards Meropenem and Ciprofloxacin. Keywords: Ganga water, microbiological quality, Most Probable Number (MPN) of Coliform, MPN Faecal coliform, Escherichia coli, Antibiotic susceptibility and resistance

scholarly journals Microbiological counting in lamb carcasses from an abattoir in São Paulo, Brazil

2009 ◽  
Vol 39 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
Thaís Mioto Martineli ◽  
Oswaldo Durival Rossi Junior ◽  
Natacha Deboni Cereser ◽  
Marita Vedovelli Cardozo ◽  
Cristianne Lino Fontoura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Sao Paulo ◽  
Most Probable Number ◽  
São Paulo ◽  
Probable Number ◽  
Lamb Meat ◽  
Thermotolerant Coliforms ◽  
Sanitary Conditions

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.

A Bacteriological Survey of Raw Ground Beef1

1977 ◽  
Vol 40 (11) ◽  
pp. 790-794 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
WARREN C. LADIGES
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Retail Store ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Fecal Streptococci ◽  
Plate Counts ◽  
Aerobic And Anaerobic

The microbiological quality of 150 units of raw ground beef obtained from a local retail store was determined. The range of aerobic plate counts was from 6.9 × 104 to 8.3 × 107/g. By using the most probable number method 96.7% of the 150 units were positive for coliforms, 94.7% for Escherichia coli and 61.3% for Staphylococcus aureus. By the plate methods, 99.3% of the units were positive for fecal streptococci and 56% were positive for Clostridium perfringens. No salmonellae were isolated. Aerobic and anaerobic organisms were isolated and identified. E. coli was the most frequently isolated aerobe followed by organisms in the Klebsiella-Enterobacter group. Among the anaerobic isolates, C. perfringens was the organism most frequently encountered.

scholarly journals Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

Pathogens ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 13 ◽  
Author(s):  
Adriana P. Januário ◽  
Clélia N. Afonso ◽  
Susana Mendes ◽  
Maria J. Rodrigues
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Coastal Waters ◽  
Enteric Bacteria ◽  
Most Probable Number ◽  
Coastal Current ◽  
Strong Positive Correlation ◽  
Probable Number ◽  
E Coli ◽  
Intestinal Enterococci

To estimate the quality of coastal waters, European Union Directive 2006/7/EC provides guidelines to assess levels of faecal bacteria, including Escherichia coli and intestinal enterococci. These microbiological criteria are based on studies that determine the risk of bathers having diseases caused by enteric bacteria, not necessarily measuring the potential danger associated with the presence of nonenteric pathogens. The association between the presence of faecal contaminant indicators and nonenteric pathogenic microorganisms has not been well defined yet. The purpose of this study is to establish a relationship between Pseudomonas aeruginosa and microbiological indicators of faecal contamination. Presence of microbiological contamination in the coastal waters near the sewage treatment plant (STP) of Peniche (Portugal) was confirmed (P. aeruginosa 135.8 Colony Forming Unit/100 mL, Escherichia coli 1100.1 Most Probable Number/100 mL, intestinal enterococci 2685.9 MPN/100 mL) with much lower levels in the areas located south of the STP, along the main water coastal current (beach 1: 0.7 CFU/100 mL, 16.5 MPN/100 mL, 100.5 MPN/100 mL; beach 2: 0.3 CFU/100 mL, 74.0 MPN/100 mL, 145.9 MPN/100 mL, respectively). Analysis of Pearson’s correlation revealed a strong positive correlation between E. coli and P. aeruginosa, suggesting E. coli as an indicator of P. aeruginosa presence.

Comparison of culturable fecal coliforms and Escherichia coli enumeration in freshwaters

2007 ◽  
Vol 53 (6) ◽  
pp. 798-801 ◽  
Author(s):  
Tamara Garcia-Armisen ◽  
Josué Prats ◽  
Pierre Servais
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Quality Data ◽  
Plate Count ◽  
Most Probable Number ◽  
Tetrazolium Chloride ◽  
Probable Number ◽  
E Coli ◽  
Glucuronidase Activity

Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detection of glucuronidase activity. FC and E. coli counts were highly correlated; an average E. coli/FC ratio equal to 0.77 was found, meaning that on average, 77% of FC were E. coli. Knowing the E. coli/FC ratio allows us to convert the historical microbiological quality data expressed in FC counts into E. coli abundance and thus to compare with present and future monitoring data that are (or will be) based on E. coli enumeration.

Environmental Dust Exposure as a Factor Contributing to an Increase in Escherichia coli O157 and Salmonella Populations on Cattle Hides in Feedyards

2008 ◽  
Vol 71 (10) ◽  
pp. 2078-2081 ◽  
Author(s):  
M. F. MILLER ◽  
G. H. LONERAGAN ◽  
D. D. HARRIS ◽  
K. D. ADAMS ◽  
J. C. BROOKS ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Positive Sample ◽  
Dust Exposure ◽  
Most Probable Number ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
Airborne Dust ◽  
E Coli ◽  
Dust Generation ◽  
The Impact

A study was conducted to determine the impact of exposure to dust in the cattle load-out area in feedyards on pathogen contamination of cattle hides. A total of 250 cattle hides were sampled during summer and fall months, which are associated with elevated prevalence of Escherichia coli O157 in West Texas. Animals were removed from their home pens and restrained in a chute and sampled prior to exposure to dust generated as a result of a simulated loading exercise. The cattle hides were sampled again after exposure to the loading dust to determine total numbers of pathogens on cattle hides on leaving their home pen (before loading) and on cattle hides after exposure to the dust in the loading area. Air and dirt samples from the home pens and the cattle load-out area were also collected. The presence of E. coli O157 and Salmonella was determined in all the samples, and when a positive sample was identified, the total numbers of these bacteria present were enumerated. The total numbers of pathogens increased after dust exposure; Salmonella counts increased from 1.09 log most probable number (MPN)/cm2 to 1.74 log MPN/cm2 after exposure, and E. coli O157 counts increased from 0.80 to 2.35 log MPN/cm2 after sampling. E. coli O157 and Salmonella were recovered from the air samples during dust generation at 6.66 and 11.1%, respectively. Salmonella and E. coli O157 prevalence was not changed and was not associated with the exposure to the dust. Results indicate airborne dust generated as a result of cattle movement and loading could be an important determining factor in total numbers of pathogens recovered on cattle hides.

scholarly journals Microbiological quality analysis of fresh vended fruit juices and water sold in roadside stalls in Dhaka Metropolis by MPN method

2017 ◽  
Vol 7 (1) ◽  
pp. 1-6
Author(s):  
Mahamuda Akther Eva ◽  
Shawda Shafiq Shreya ◽  
Tasnia Ahmed
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Microbiological Quality ◽  
Test Method ◽  
Quality Analysis ◽  
Most Probable Number ◽  
Gram Negative Bacteria ◽  
Probable Number ◽  
Gram Negative

Quality of drinking water and juice is very important because if the quality deteriorates by the contamination of faecally originated microorganisms, it may cause serious diarrhea associated problems leading to death. In overpopulated countries like Bangladesh, this is a common scenario to experience diarrheal diseases due to drinking non-potable water as well as contaminated fresh juices. Present study was conducted to determine the quality of drinking water and juice by detection of indicator bacteria Escherichia coli by MPN (Most Probable Number) method which was performed by three consecutive steps including presumptive test, confirmed test and completed test. Other gram negative bacteria were also identified by biochemical methods. The indicator bacterium Escherichia coli was detected in two water samples out of 15 samples and one juice sample out of fifteen samples respectively during the MPN test method. Other Gram negative bacteria found in both water and juice samples included Klebsiella spp., Alcaligenes spp., Pseudomonas spp. and Proteus spp. The quality of drinking water and juice was found to be good in Bangladesh but proper hygiene should be maintained more strictly to avoid the contamination by other gram negative bacteria which are also capable of causing disease. Stamford Journal of Microbiology, Vol.7(1) 2017: 1-6

scholarly journals Antibiotics resistant Escherichia coli isolated from aquatic ecosystems in Palembang, South Sumatra, Indonesia

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Marieska Verawaty ◽  
NITA APRIANI ◽  
LELY RASTI TARIGAN ◽  
ENDAH TRI APRIAN ◽  
WEMONA CHARISSA LAURENTA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Aquatic Ecosystems ◽  
Antimicrobial Agents ◽  
Quality Standard ◽  
Most Probable Number ◽  
Resistant Bacteria ◽  
Fish Farms ◽  
Water Contaminants ◽  
Probable Number ◽  
E Coli

Abstract. Verawaty M, Apriyani N, Tarigan LR, Apriyan ET, Laurenta WC, Apriani N, Muharani. 2020. Antibiotics resistant Escherichia coli isolated from aquatic ecosystems in Palembang, South Sumatra, Indonesia. Biodiversitas 21: 86-97. Antibiotics-resistant bacteria (ARB) are one of the emerging water contaminants currently gaining serious global concern due to their adverse risk to ecosystems, wildlife, and public health. This study determined antibiotics resistant Escherichia coli isolated from aquatic ecosystems in Palembang. Most probable number (MPN) was used for bacterial estimation and the Kirby-Bauer method was used for susceptibility test against antimicrobial agents. The results indicated that 82% of E. coli isolates from 28 sampling sites were resistant to ampicillin, 57% to tobramycin, and 71% to tetracycline. The isolates showed intermediate profile to kanamycin (50%), 57% to cotrimoxazole, 50% to cefixime, and 54% to gentamycin. These isolates still showed sensitivity towards ciprofloxacin (86%) and chloramphenicol (61%). Total coliform (TC) numbers ranged from 0 to >1600 MPN/100 mL. Sampling sites with high MPN values of ≥1600 MPN/100 mL were Sekanak watersheds (SW1, SW8, SW11, SW12, SW13, and SW14) followed by SW2 and SW9 with a value of 1600 MPN/100 mL, while samples from cattle and fish farms (CW) varied from 0 to 170.000 MPN/100 mL. TC of samples collected from retention ponds (RP) ranged from 0 (RPJSC) to 1.600.000 MPN/100 mL (RPSH3). The Escherichia coli (EC) counts varied from 1.7 x 103 (RPSH2) to ≥1.6 × 104 MPN/100 mL (SW11, SW12, SW13, and SW14). Several samples (SW3, RPPI, RPTS, RPSB, RPIBA, and RPOPI) have no E. coli. The results indicated some of the sampling locations that exceeds the quality standard of water have been regulated by the Governor of South Sumatra and the Indonesian Government.

scholarly journals Distribution of multidrug-resistant Salmonella spp. recovered from aquatic environment of Banda Aceh, Indonesia

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Suhartono Suhartono ◽  
Yulia Sari Ismail ◽  
Zahratul Aini
Keyword(s):  
Aquatic Environment ◽  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Most Probable Number ◽  
Salmonella Spp ◽  
Potential Health ◽  
Probable Number ◽  
Antibiotic Susceptibility Test ◽  
Banda Aceh

Abstract. Suhartono S, Ismail YS, Aini Z. 2021. Distribution of multidrug-resistant Salmonella spp. recovered from aquatic environment of Banda Aceh, Indonesia. Biodiversitas 22: 881-886. The occurrence of pathogenic bacteria in an aquatic system might increase waterborne infections including salmonellosis. This might raise a concern related to the increasing pathogenic bacteria, including the multidrug-resistant Salmonella spp. in the two major rivers, namely Lamnyong and Krueng Aceh crossing the city of Banda Aceh, Indonesia. The objectives of the present study were to isolate and enumerate Salmonella spp. recovered from aquatic environment in Banda Aceh and to determine their antibiotic susceptibility. Bacterial enumeration was performed using most probable number (MPN) method followed by bacterial isolations using selective media Salmonella-Shigella Agar (SSA); whereas, antibiotic susceptibility test was conducted using a procedure as recommended by the National Committee for Clinical Laboratory Standards. There was no significant Salmonella spp. densities/mL of both locations (p = 0.200) although, on average there was about 441.67 Salmonella spp. MPN/mL in Krueng Aceh as opposed to 20.47 MPN/mL of Lamnyong river. There were 57 Salmonella spp. isolates recovered in the sampling sites, i.e. 38 isolates of Lamnyong and 19 isolates of Krueng Aceh. The results of antibiotic susceptibility tests showed that Salmonella spp. isolates recovered from Lamnyong and Krueng Aceh showed antibiotic resistance to azithromycin, tetracycline, and streptomycin. Moreover, Salmonella spp. isolates also showed high resistance to ofloxacin, ciprofloxacin, dan ceftriaxone. All Salmonella spp. isolates were classified as MDR with the number of isolates resistant to 4-6 antibiotics was 33 (57.9%), whereas those that were resistant to 7-9 antibiotics and over 10 antibiotics were 23 (40.4%%) and 5 (8.8%) respectively. The prevalence of MDR Salmonella spp. in the current study suggest that river remain the major reservoirs for the transmission of the pathogen in aquatic systems. An appropriate assessment and monitoring are necessary to reduce the potential health risks and to ensure water quality in the aquatic environment.

Sign in / Sign up

Export Citation Format

Share Document