In Vitro
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2021 ◽  
Vol 233 (5) ◽  
pp. e208-e209
Jamie B. Hadley ◽  
Marguerite R. Kelher ◽  
Julia R. Coleman ◽  
Margot DeBot ◽  
Andrew P. Eitel ◽  
In Vitro ◽  

2021 ◽  
Vol 87 ◽  
pp. 104796
Xiaoling Li ◽  
Meicui Chen ◽  
Zhicui Yao ◽  
Hongzhen Du ◽  
Tianfeng Zhang ◽  

2021 ◽  
Vol 101 ◽  
pp. 108274
Alireza Keyhani ◽  
Iraj Sharifi ◽  
Ehsan Salarkia ◽  
Ahmad Khosravi ◽  
Razieh Tavakoli Oliaee ◽  

Shivangi Shrivastava

Abstract: The in-silico approach is common in today’s world. As it provide a vast knowledge of hypothetical world which have to be proven by undergoimg in in-vitro conditions. There are much data is available on databases which helps to complete the future study related to medicine, environment and nano-technology. The study covered the new ideas which can able to change the approach of biosynthesis of PHB in microorganisms and degradation of biopolymer without any harmful effect on environment as well as ecosystem. Keywords: Polyhydroxybutyrate, Phylogeny tree, Polyesters, Biodegradable, Biosynthesis, Rlastonia eutropha

2021 ◽  
Vol 144 ◽  
pp. 112342
Magdalena Czarnecka-Czapczyńska ◽  
David Aebisher ◽  
Piotr Oleś ◽  
Barbara Sosna ◽  
Magdalena Krupka-Olek ◽  

2021 ◽  
Vol 17 (1) ◽  
pp. 160-175
Keziah Uchechi Ajah ◽  
Ozioma Anne Asomugha ◽  
Chinazom Perpetua Ifedigbo ◽  
Kosoluchukwu Chidubem Umeh ◽  
Onyeka Chinwuba Obidiegwu ◽  

Isoflavones and flavonoids in general found in fruits and vegetable act as natural antioxidants. This study was aimed at screening the isoflavone-rich fractions of Vitex doniana fruit extract for antioxidant activity using in vitro and in vivo models. The ethyl acetate and butanol fractions were screened for in vitro antioxidant activity by DPPH free radical scavenging and FRAP (Fe3+ Reducing Antioxidant Power) assays using ascorbic acid as standard. In vivo antioxidant activity was also tested against carbon tetrachloride-induced toxicity in mice using carbon tetrachloride (4 mL/kg) and silymarin (100 mg/kg) as standard. The ethyl acetate fraction and butanol Vacuum Liquid Chromatography (VLC) sub-fractions were subjected to High Performance Liquid Chromatography–Diode Array Detector (HPLC-DAD) and Liquid Chromatography-Mass Spectrometry (LC-MS) analyses. The ethyl acetate and butanol fractions inhibited DPPH radicals with an IC50 of 897 µg/mL and 2809.38 µg/mL respectively. Both fractions also showed mild FRAP result with EC50 of 1401 µg/mL and 7051 µg/mL respectively. The fractions produced significant decrease (P < 0.05) in ALP levels only while ethyl acetate fraction caused a significant reduction in the value of only malondialdehyde. Both fractions also increased levels of superoxide dismutase (SOD). HPLC-DAD analysis led to the detection of cinnamic acid, protocatechuic acid and two isoflavones daidzein and genistein. Further LC-MS analysis confirmed the presence of the isoflavones tectorigenin, 5-O-methylgenistein, and 5-O-methyltectorigenin, among other flavonoids, peonidin, 5-methylpeonidin, methylmalvidin and kaempferol-3-O-(2''galloyl)-glucopyranoside. The detected isoflavones and other flavonoids may contribute to the observed mild in vitro and good in vivo antioxidant activity of V. doniana fruit extract and fractions.

2021 ◽  
Vol 1 (4) ◽  
pp. 48-54
Kumari Meenakshi ◽  

The present study was conducted at Central Research Potato Institute, Campus, Modipuram, India to assess the effect of kinetin on the growth and development of microtubers formation in vitro in two potato varieties Kufri Bahar and Kufri Surya. The lower concentration of kinetin (0.75 mg/l) showed decreased parameters like the number of microtubers, fresh weight of microtubers, eyes per microtuber. Higher concentration of kinetin (1.5 mg/l and 2.25 mg/l) led to increasing parameters as compared to control but decreased shoot length in both the varieties but kinetin at (2.25 mg/l) concentration gave best results for in vitro microtuberization for quality and yield parameters.

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