Bath for Underwater Horizontal Polysegmental Kinesitraction Treatment of the Spine and Lower Extremities Lesions, Methods of Its Use

Introduction. Traction in dynamic mode (kinesitraction) is a new direction protected by patents of Ukraine, which combines the procedure of traction and motor activity. Traction treatment of the spine in a dynamic mode plays an important role in the treatment of the musculoskeletal system, so it is important to understand the structure, mechanisms and features of the structures involved in these processes, including the bath developed by us. The aim of the study. To acquaint with a design of a bath for underwater horizontal polysegmental kinesitraction treatment of the spine and lower extremities lesions, methods of its use. Materials and methods. Content analysis, method of system and comparative analysis, bibliosemantic method of studying the actual scientific researches on baths’ designs for underwater horizontal polysegmental kinesitraction treatment of the spine and lower extremities lesions, methods of its use are used. Sources are searched in scientometric databases: PubMed, Medline, Springer, Google Scholar, Research Gate by tags: kinesitraction, musculoskeletal system, underwater horizontal treatment of the spine, traction, traction system. 22 English and Ukrainian literary sources which describe this problem were selected and analyzed. The description of the bath for underwater horizontal segmental traction treatment of the spine and lower extremities lesions in the dynamic mode was used (Certificate of copyright registration for a scientific work N 99985, 25.10.2020). Results. The designed by us bath for underwater polysegmental kinesitraction treatment of lesions of the spine and lower extremities (Certificate of registration of copyright to a scientific work N 99985, 2020) is intended for the treatment of diseases of the musculoskeletal system, internal organs, disorders of vital systems in the aquatic environment by exposure to dosed motor and traction loads. The design includes a water bath, armrests, handles, footrest, racks, cervical block system, chest block system, lumbar block system, head restraint belt, chest strap, pelvic girdle, shin strap, cable, load. Using a bath it is possible to carry out tractions of the cervical, thoracic, lumbar spine, their combinations, lower extremities, or total provide the traction. Conclusions. Traction treatment of the spine in a dynamic mode plays an important role in the treatment of the musculoskeletal system, being more effective than static. The understanding of the structure, methods and features of the structures involved in these processes, including our developed baths for underwater horizontal polysegmental hydrokinesitraction treatment of lesions of the spine and lower extremities, based on the correction of disorders of the nervous, musculoskeletal systems caused by morphological, biochemical, physiological mechanisms of stimulation of the functions of vital systems, regeneration processes, and the proposed methods of their use is an important component of effective treatment of diseases of the musculoskeletal system. Keywords: kinesitraction, musculoskeletal system, underwater horizontal treatment of the spine, traction, traction system.

A Novel Assay Allowing Drug Self-Administration, Extinction, and Reinstatement Testing in Head-Restrained Mice

Multiphoton microscopy is one of several new technologies providing unprecedented insight into the activity dynamics and function of neural circuits. Unfortunately, some of these technologies require experimentation in head-restrained animals, limiting the behavioral repertoire that can be integrated and studied. This issue is especially evident in drug addiction research, as no laboratories have coupled multiphoton microscopy with simultaneous intravenous drug self-administration, a behavioral paradigm that has predictive validity for treatment outcomes and abuse liability. Here, we describe a new experimental assay wherein head-restrained mice will press an active lever, but not inactive lever, for intravenous delivery of heroin or cocaine. Similar to freely moving animals, we find that lever pressing is suppressed through daily extinction training and subsequently reinstated through the presentation of relapse-provoking triggers (drug-associative cues, the drug itself, and stressors). Finally, we show that head-restrained mice will show similar patterns of behavior for oral delivery of a sucrose reward, a common control used for drug self-administration experiments. Overall, these data demonstrate the feasibility of combining drug self-administration experiments with technologies that require head-restraint, such as multiphoton imaging. The assay described could be replicated by interested labs with readily available materials to aid in identifying the neural underpinnings of substance use disorder.

A miniature kinematic coupling device for mouse head fixation

Head-fixation is a common technique in the preparation of subjects for neuroscience experiments. Accurate alignment, stability, and repeatability during fixation provide experimental consistency, thus enabling the subject to return to the same position over time to provide meaningful data. Head restraint systems inspired by kinematic clamps have been developed to allow micron scale repositioning across imaging epochs in rats. Here we report the development of a light-weight, implantable kinematic coupling (clamp) system that is wearable by mice, and enables repeated positioning to submicron accuracy across imaging epochs. This system uses a stainless steel headplate and a Maxwell-style three-groove kinematic mounting system with magnetic force clamping load. Spheres on the dorsal surface of the headplate provide contact points for vee-groove kinematic features machined into a tabletop mount. Evaluation of the clamp using multiphoton microscopy revealed submicron precision in registration accuracy and stability, allowing cellular resolution calcium imaging in awake, behaving mice. These results indicate that miniaturized implantable kinematic clamps for mice could be valuable for future experiments which require repositioning of subjects across time and different instruments.

Thoracic weighting of restrained subjects during exhaustion recovery causes loss of lung reserve volume in a model of police arrest

Restraint asphyxia has been proposed as a mechanism for some arrest-related deaths that occur during or shortly after a suspect is taken into custody. Our analysis of the literature found that prone positioning, weight applied to the back, recovery after simulated pursuit, and restraint position have led to restrictive, but non life-threatening respiratory changes when tested in subsets. However, the combined effects of all four parameters have not been tested together in a single study. We hypothesized that a complete protocol with high-sensitivity instrumentation could improve our understanding of breathing physiology during weighted restraint. We designed an electrical impedance tomography (EIT)-based protocol for this purpose and measured the 3D distribution of ventilation within the thorax. Here, we present the results from a study on 17 human subjects that revealed FRC declines during weighted restrained recovery from exercise for subjects in the restraint postures, but not the control posture. These prolonged FRC declines were consistent with abdominal muscle recruitment to assist the inspiratory muscles, suggesting that subjects in restraint postures have increased work of breathing compared to controls. Upon removal of the weighted load, lung reserve volumes gradually increased for the hands-behind-the-head restraint posture but continued to decrease for subjects in the hands-behind-the-back restraint posture. We discuss the possible role this increased work of breathing may play in restraint asphyxia.

Design and Evaluation of the Initial 50th Percentile Female Prototype Rear Impact Dummy, BioRID P50F – Indications for the Need of an Additional Dummy Size

The objective of this study was to present the design of a prototype rear impact crash test dummy, representing a 50th percentile female, and compare its performance to volunteer response data. The intention was to develop a first crude prototype as a first step toward a future biofidelic 50th percentile female rear impact dummy. The current rear impact crash test dummy, BioRID II, represents a 50th percentile male, which may limit the assessment and development of whiplash protection systems with regard to female occupants. Introduction of this new dummy size will facilitate evaluation of seat and head restraint (HR) responses in both the average sized female and male in rear impacts. A 50th percentile female rear impact prototype dummy, the BioRID P50F, was developed from modified body segments originating from the BioRID II. The mass and rough dimensions of the BioRID P50F is representative of a 50th percentile female. The prototype dummy was evaluated against low severity rear impact sled tests comprising six female volunteers closely resembling a 50th percentile female with regard to stature and mass. The head/neck response of the BioRID P50F prototype resembled the female volunteer response corridors. The stiffness of the thoracic and lumbar spinal joints remained the same as the average sized male BioRID II, and therefore likely stiffer than joints of an average female. Consequently, the peak rearward angular displacement of the head and T1, and the rearward displacement of the T1, were lesser for the BioRID P50F in comparison to the female volunteers. The biofidelity of the BioRID P50F prototype thus has some limitations. Based on a seat response comparison between the BioRID P50F and the BioRID II, it can be concluded that the male BioRID II is an insufficient representation of the average female in the assessment of the dynamic seat response and effectiveness of whiplash protection systems.

The Changes of Ergonomic Engine Vibroacoustic Response Regarding Their Development

The article presents the results of research on the vibroacoustic response of internal combustion engines mounted in a vehicle. The vehicles studied belong to popular models, which became available in successive versions. Each group included vehicles of the same model of an older generation (equipped with a naturally aspirated engine) and of a newer generation, including downsized (and turbocharged) engines. Tests in each group were carried out under repeatable conditions on a chassis-load dynamometer. The vibrations were measured using single-axis accelerometers mounted on the steering wheel, engine, and driver’s head restraint mounting. The primary purpose of the study was to verify whether the new generations of vehicles equipped with additional high-speed elements (compressors) generate additional harmonics (especially those within the range potentially affecting travel comfort and human health) and whether there are significant changes in the distribution of spectral power density in the new generations. As the study showed, new generations of vehicles are characterized by a different vibroacoustic response, and the trend of change is the same in each of the families studied.

Dynamic Responses of Female Volunteers in Rear Impact Sled Tests at Two Head Restraint Distances

The objective of this study was to assess the biomechanical and kinematic responses of female volunteers with two different head restraint (HR) configurations when exposed to a low-speed rear loading environment. A series of rear impact sled tests comprising eight belted, near 50th percentile female volunteers, seated on a simplified laboratory seat, was performed with a mean sled acceleration of 2.1 g and a velocity change of 6.8 km/h. Each volunteer underwent two tests; the first test configuration, HR10, was performed at the initial HR distance ∼10 cm and the second test configuration, HR15, was performed at ∼15 cm. Time histories, peak values and their timing were derived from accelerometer data and video analysis, and response corridors were also generated. The results were separated into three different categories, HR10C (N = 8), HR15C (N = 6), and HR15NC (N = 2), based on: (1) the targeted initial HR distance [10 cm or 15 cm] and (2) whether the volunteers’ head had made contact with the HR [Contact (C) or No Contact (NC)] during the test event. The results in the three categories deviated significantly. The greatest differences were found for the average peak head angular displacements, ranging from 10° to 64°. Furthermore, the average neck injury criteria (NIC) value was 22% lower in HR10C (3.9 m2/s2), and 49% greater in HR15NC (7.4 m2/s2) in comparison to HR15C (5.0 m2/s2). This study supplies new data suitable for validation of mechanical or mathematical models of a 50th percentile female. A model of a 50th percentile female remains to be developed and is urgently required to complement the average male models to enhance equality in safety assessments. Hence, it is important that future protection systems are developed and evaluated with female properties taken into consideration too. It is likely that the HR15 test configuration is close to the limit for avoiding HR contact for this specific seat setup. Using both datasets (HR15C and HR15NC), each with its corresponding HR contact condition, will be possible in future dummy or model evaluation.

Prediction of Neural Space Narrowing and Soft Tissue Injury of the Cervical Spine Concerning Head Restraint Arrangements in Traffic Collisions

Common quantitative assessments of neck injury criteria do not predict anatomical neck injuries and lack direct relations to design parameters of whiplash-protection systems. This study aims to provide insights into potential soft tissue-level injury sites based on the interactions developed in-between different anatomical structures in case of a rear-end collision. A detailed finite element human model has exhibited an excellent biofidelity when validated against volunteer impacts. Three head restraint arrangements were simulated, predicting both the kinematic response and the anatomical pain source at each arrangement. Head restraint’s contribution has reduced neck shear and head kinematics by at least 70 percent, minimized pressure gradients acting on ganglia and nerve roots less than half. Posterior column ligaments were the most load-bearing components, followed by the lower intervertebral discs and upper capsular ligaments. Sprain of the interspinous ligamentum flavum at early stages has caused instability in the craniovertebral structure causing its discs and facet joints to be elevated compressive loads. Excessive hyperextension motion, which occurred in the absence of the head restraint, has promoted a stable avulsion teardrop fracture of the fourth vertebral body’s anteroinferior aspect and rupture the anterior longitudinal ligament. The observed neck injuries can be mathematically related to head–torso relative kinematics. These relations will lead to the development of a comprehensive neck injury criterion that can predict the injury level. This, in turn, will impose a significant impact on the design processes of vehicle anti-whiplash safety equipment.