scholarly journals Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

2021 ◽  
Author(s):  
M.L Avinash Tejasvi ◽  
Jaya Laksmi Bukkya ◽  
Pandu Ranga Rao ◽  
Harsha Bhayya
Keyword(s):  
Blood Group ◽  
Saliva Sample ◽  
Test Method ◽  
Control Group ◽  
Blood Group Antigens ◽  
Slide Agglutination ◽  
Secretor Status ◽  
Agglutination Method ◽  
Group 5 ◽  
And Control

Abstract Objectives While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample. Materials and Methods A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method Results Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females. Conclusion Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.

scholarly journals THE HISTOLOGICAL DISTRIBUTION OF BLOOD GROUP SUBSTANCES A AND B IN MAN

1960 ◽  
Vol 111 (6) ◽  
pp. 785-800 ◽  
Author(s):  
Aron E. Szulman
Keyword(s):  
Blood Group ◽  
Fluorescent Antibody ◽  
Sweat Glands ◽  
Surgical Removal ◽  
Positive Staining ◽  
Fluorescent Antibody Technique ◽  
Blood Group Antigens ◽  
Specific Staining ◽  
Antibody Technique ◽  
Secretor Status

The mapping out of the histologic distribution of blood group antigens A and B in human tissues was performed by means of the fluorescent antibody technique. Human hyperimmune sera were conjugated with fluorescein isocyanate and applied to frozen sections of human material obtained at autopsy or after surgical removal. The material examined encompassed A, B, and AB subjects. In the latter the anti-A and the anti-B conjugate elicited the same picture. Group O tissues were used for controls and were uniformly negative. The secretor status of subjects was determined from the saliva or by the Lewis typing of erythrocytes. The results fall into the following main divisions: Endothelia of Vessels.—Widespread localization was demonstrated in the cell walls of endothelium of capillaries, veins, arteries, and of sinusoidal cells of spleen. Stratified Epithelia.—These showed good outlining of cells of the Malpighian (and the granular, when present) layers. In transitional epithelia, cells of the basal and contiguous layers gave specific staining. Mucus-Secreting Apparatus.—Positive staining was obtained in glands, goblet cells, and secreting surface epithelia. In non-secretors there was no identifiable antigen with the important exception of the deeper parts of gastric foveolae, deeper parts of crypts of Lieberkühn of bowel mucosa and Brunner's glands of the duodenum. Various Organs of Secretion and Excretion.—The pancreas (exocrine portion) and the sweat glands were found to produce the antigen irrespectively of secretor status. Breast, prostate, and endometrial glands on the other hand apparently secrete the antigen in conformity with the subject's secretor:non-secretor make-up. Thus the secretor:non-secretor status governs principally the antigens associated with mucous secretions and this in most but not all locations. The possible nature of this control is briefly discussed.

Immunocytochemical localization of Lewis blood group antigens in human salivary glands.

1994 ◽  
Vol 42 (8) ◽  
pp. 1135-1142 ◽  
Author(s):  
M Cossu ◽  
M S Lantini ◽  
R Puxeddu
Keyword(s):  
Salivary Glands ◽  
Blood Group ◽  
Secretory Granules ◽  
Ultrastructural Level ◽  
Blood Group Antigens ◽  
Immunogold Staining ◽  
Secretor Status ◽  
Duct Cells ◽  
Granular Matrix ◽  
B Blood Group

We demonstrated the immunohistochemical distribution of Le-a and Le-b blood group antigens in human major and minor salivary glands at the ultrastructural level by applying a post-embedding immunogold staining method. In secretors' glands, a faint Le-a reactivity was found only in mucous droplets, whereas Le-b antigen was intensely stained in secretory granules of most mucous cells, in those of intercalated duct cells, in the pale granular matrix of some serous cells, and, when osmication was omitted, in cytoplasmatic vesicles and cell surfaces of striated ducts. In the submandibular gland of a non-secretor, Le-a antigen was considerably stained in mucous droplets, whereas Le-b reactivity was restricted to the striated duct cells. These results indicate that the secretor status affects the secretion of Lewis antigens by mucous, serous, and intercalated duct cells but not the presence of Le-b as a surface antigen in striated duct cells.

scholarly journals Effect of Donor and Recipient ABH-Secretor Status on ABO-Incompatible Living Donor Kidney Transplantation

2021 ◽  
Vol 12 ◽  
Author(s):  
Fan Zhang ◽  
Saifu Yin ◽  
Yu Fan ◽  
Turun Song ◽  
Zhongli Huang ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Blood Group ◽  
Graft Rejection ◽  
Living Donor ◽  
Blood Group Antigens ◽  
Post Transplantation ◽  
Donor Kidney ◽  
Secretor Status ◽  
Donor Kidney Transplantation ◽  
Living Donor Kidney

IntroductionABO blood group antigens within grafts are continuously exposed to anti-A/B antibodies in the serum of recipients after ABO-incompatible (ABOi) kidney transplantation and are instrumental in antibody-mediated rejection. Some individuals secrete soluble blood group antigens into body fluids. In this study, we investigated the effect of donor and recipient secretor status on the outcomes of ABOi kidney transplantation.MethodsData of a total of 32 patients with ABOi living donor kidney transplantation were retrospectively collected between 2014 and 2020 in West China Hospital. The genotype and phenotype of both donors and recipients were examined and evaluated with post-transplantation anti-A/B titer changes, graft function, and rejection.ResultsOf the 32 recipients and 32 donors, 23 (71.9%) recipients and 27 (84.4%) donors had secretor genotypes, whereas 9 (28.1%) recipients and 5 (15.6%) donors did not. Anti-A/B titers after ABOi kidney transplantation were not significantly influenced by the secretor status of either donors or recipients. The post-transplantation serum creatinine (Scr) levels and estimated glomerular filtration rate (eGFR) was better in weak- or non-secretor recipients at day 30 (Scr P = 0.047, eGFR P = 0.008), day 90 (Scr P = 0.010, eGFR P = 0.005), and month 9 (eGFR P = 0.008), and recipients from secretor donors had a lower incidence of graft rejection in the first year after ABOi transplantation (P = 0.004).ConclusionsA weak secretor status phenotype was found in both genotypes, i.e., individuals who secreted soluble antigens as well as those who did not. The recipient ABH-secretor status may have an influence on early posttransplant renal function, and the donor ABH-secretor status might affect the incidence of graft rejection.

scholarly journals Association between secretor status and Lewis phenotype with seronegative spondyloarthritis as indicator of autoimmunity

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 127-136
Author(s):  
Ivan Busarcevic ◽  
Svetlana Vojvodic ◽  
Una Vojvodic
Keyword(s):  
Blood Group ◽  
Haemagglutination Inhibition ◽  
Gastrointestinal Symptoms ◽  
Blood Group Antigens ◽  
Control Subjects ◽  
Secretor Status ◽  
Lewis Blood Group ◽  
Increased Risk ◽  
Significant Difference ◽  
Seronegative Spondyloarthropathies

The classical paradigm of autoimmune pathogenesis involving specific genetic makeup and exposure to environmental triggers has been challenged recently by the addition of a third element, the loss of intestinal barrier function. Regardless of HLA B27 phenotype or gastrointestinal symptoms, evidence of ileitis, ileocolitis or colitis exists in patients with spondyloarthropathy. The FUT2 secretory gene is a strong candidate for Crohn's susceptibility by shaping the functional states of mucosal microbiota and may thus have influence on the release of zonulin, the main regulator of gut permeability. Gram negative bacteria precipitate and may be involved in the pathogenesis of spondyloarthropathies. Susceptibility to many infectious agents is associated with ABO blood group or secretor state. Patients who cannot secrete ABO and Lewis blood group antigens into body fluids, an ability controlled by a single gene on chromosome 19, are known to be at increased risk of certain autoimmune diseases associated with human leukocyte antigen (HLA) markers. Lewis (Le) blood group phenotype can be used to infer secretor status. The objective of this study was to determine the distribution of secretor state and Lewis blood group phenotype in patients with seronegative spondyloarthropathies and healthy control subjects. Hundred and ten (110) patients with seronegative spondyloarthropathies (58 females and 52 males) and 103 control (74 males and 29 females) subjects participated in this study. Samples of saliva and blood were subjected to haemagglutination inhibition tests for determination of secretor status and Lewis phenotype. A total of 92(84%) patients and 92 (89%) control subjects were secretors while 18 (16%) patients and 11 (11%) control subjects were non-secretors. There was no statistically significant difference (?2 1,461 p<0,05 and degrees of freedom 1) in distribution of secretor status in comparison to seronegative spondyloarthropathies by comparing two observed populations. Seven patients had modified (reduced) expression of Lewis b antigen on their erythrocytes. Reduction of Lewis b antigen expression was not observed on erythrocytes of healthy subjects. Reduced expression of Lewis b antigen could be a consequence of the inflammatory process within the gut and it also suggests several pathogenic mechanisms which may be relevant to the synthesis of Lewis antigens inside the gut or its absorption on erythrocytes in patients with spondyloarthropathy.

scholarly journals Replacement of vitamin-mineral premix by Spirulina and its effect on the performance of broiler

2018 ◽  
Vol 2 ◽  
pp. 39
Author(s):  
Shahinur Nahar Moury ◽  
Md. Touhiduzzaman Sarker ◽  
Adhita Sri Prabakusuma ◽  
Md. Imrul Hasan Russel ◽  
Md. Shahidul Islam
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Group 4 ◽  
Group 6 ◽  
Group 5 ◽  
And Control ◽  
Group 1

The main purpose of this study was to explore the effects of Spirulina (Spirulina platensis) as a feed supplement by replacing Vitamin-Mineral Premix on the performance of broiler. The study explored that final body weight was 1039, 1070, 1044, 1065, 1117 and 893 g/bird in group 1, 2, 3, 4, 5 and 6 where significantly (p<0.5) higher weight observed in Spirulina group (5) but lower in negative control group (6). Feed intake observed more or less similar in all groups (1483±24g; p>0.5). Feed conversion ratio (kg FI/kg LWG) observed significantly different like 1.72, 1.65, 1.69, 1.62, 1.61 and 1.99 in group 1, 2, 3, 4, 5 and 6 respectively (p<0.5) where higher in negative group (6) and control group (1), but is lowest and similar in 75% Spirulina group (4) and highest (100%) Spirulina group (5). %Ash of different dietary groups were 46.35, 46.54, 48.93, 49.93, 55.07 and 46.92 of group 1, 2, 3, 4, 5 and 6 respectively. The %ash of bone was gradually increased among dietary groups and the best result was obtained in 100% Spirulina group 5 (55.07). It also revealed that diets containing no vitamin-mineral premix with 100% Spirulina improve the performance of broiler. The findings suggest that poultry farmer can use spirulina as a feed item for broiler for more yield.

scholarly journals Comparison of acute normovolemic hemodilution effect on the amount of bleeding in the first 48 hours after coronary artery bypass grafting

2018 ◽  
Vol 5 (6) ◽  
pp. 1417
Author(s):  
Vadoud Norouzi ◽  
Mohammad Hasanpour ◽  
Sahar Khajavi
Keyword(s):  
Coronary Artery ◽  
Coronary Artery Bypass ◽  
Blood Group ◽  
Artery Bypass ◽  
Control Group ◽  
Control Groups ◽  
Bleeding Volume ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Background: Cardiovascular disease is one of the most common causes of mortality in developed countries, as well as in the whole world. In this regard, autologous transfusion is a topic that can be useful and valuable, especially in complex surgery such as heart surgery and organ transplantation. One of its variants is Acute Normovolomic Hemodilution (ANH). Therefore, the aim of this study was to compare the effect of ANH on the amount of bleeding in the first 48hours after coronary artery bypass grafting.Methods: In this clinical trial, 100 patients were selected from all heart patients referred to Imam Khomeini Hospital in Ardebil for CABG surgery in the years 2016-17. They were selected by simple random sampling as a statistical sample and They were divided into intervention (50 ANH recipients’ people) and control groups (50 without ANH people).Results: Based on the findings, in the ANH recipients’ group, the mean of bleeding volume was 59.1±7.3ml in the first day and 55.6±4.2ml in the control group and the difference between the two groups was not significant on the first day. The mean of bleeding volume on the second day was 46.1±2.8ml in the ANH group and 42.7±2.9ml in the control group. Although it was somewhat higher, it was not statistically significant. Of all samples 45% were female and 55% were male. The most common type of blood group was 33 (33%) in the blood group O. The highest age group (61%) was over the 60 years old. The pump time varied with an average of 122.2±21.5minutes. The lowest value was PT 11 and the highest was 15 with an average of 13.02±0.9seconds. The mean of PTT was 32.5±2.6seconds and the mean INR was 1.1±1.0. The average plt was 251170±64124. The mean ACT was 596 / 6±183/7seconds.Conclusions: The results showed that in the mean of bleeding volume between the intervention and control groups (p = 0.41), bleeding volume by age (p = 0.3), bleeding volume by gender (p =0.54) and bleeding volume by blood group (p=0.48) was not significant difference. Based on these results, it is suggested that more studies be done on the more number of samples.

scholarly journals Relationship of secretor status in patients with oral cancers

2016 ◽  
Vol 11 (4) ◽  
Author(s):  
Sajid Jamil ◽  
Muhammad Tayyab ◽  
Muhammad Farooq
Keyword(s):  
Oral Cancer ◽  
Saliva Sample ◽  
Control Group ◽  
Healthy Controls ◽  
Oral Cancers ◽  
Secretor Status ◽  
Relationship Of

Present study was designed to find the association of secretor status with oral cancers. In this study 50 diagnosed cases of oral cancers and 50 healthy controls were selected. Secretor status on saliva sample was performed in all these subjects. 39 (78%) subjects were secretors and 11 (22%) were non secretors in control group. 28 (56%) patients with oral cancers were secretors and 22 (44%) were non secretors. Conclusions: Oral cancer was found to be associated with non-secretor status.

scholarly journals CONVERGES OF SKY YOGA AND KAYAKALPA ON THE CORE MUSCLE STRENGTH AND AGEING FACTOR

2019 ◽  
pp. 1-2
Author(s):  
K. Krishnaveni ◽  
M. Jothilakshmi*
Keyword(s):  
The Body ◽  
Test Method ◽  
Control Group ◽  
Mental Ability ◽  
Not Given ◽  
The Core ◽  
Group I ◽  
And Control ◽  
Experimental Group ◽  
Neurological System

INTRO: Today's generation children are growing faster in all aspects mentally,physiologically and physically too than before generation.The life span of the human being got reduced to averagely 63 age,value tells more than the words and What will be the future? Are the children are not going to see their grandfather and grandmother? These are the question which lead this study to overtaken. METHOD:The college boys from in and around Coimbatore were selected as subjects for the propose of study.The total 60 subjects were selected and they age in 18 to 21 years.Then subjects were divided into three groups,each containing 20 members.Experimental Group I given SKY yoga with Kayakalpa training and Experimental Group II were given only SKY yoga training for 5 days in week for 10 weeks and control group not given any training. RESULTS:The study shown that there was improvement in the coordination of hand- mind and ability to think measured by Shuttle cross pick-up test method to the Experimental groups I & II than the subjects in control group. The coordination of hand- mind has increased this shown that SKY yoga effects the neurological system and mental ability of the body. CONCLUSION: The result shown that SKY Yoga with Kayakalpa on college students has positively impact on the core muscles and ageing factor

Salivary miRNA-210 involvement in medication-related osteonecrosis of the jaws.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e23052-e23052
Author(s):  
Antonio Picone ◽  
Antonia Marcianó ◽  
Rosanna Dimarco ◽  
Eleonora Miano ◽  
Alessandra Zacchia ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Saliva Sample ◽  
Methylation Status ◽  
Previous History ◽  
Predictor Variable ◽  
Control Group ◽  
Case Group ◽  
Osteonecrosis Of The Jaws ◽  
Health Related ◽  
And Control

e23052 Background: Improved survival rates from cancer have increased the need to understand the health related problems of cancer treatment. Persistent oral health related problems significantly affect patient's quality of life. Medication-related osteonecrosis of the jaws (MR-ONJ) is an oral complication of antiresorptive medications, denosumab and other medications that target angiogenesis for the management of metastatic bone cancer. The purpose of this pilot study, is to investigate, for the first time, microRNAs expression in MR-ONJ. Recent studies reported the miRNA-210 (a specific angiogenic-related miRNA) demethylation in the Osteonecrosis of the Femoral Head. We hypothesized that MiRNA-210 could also be involved in MR-ONJ development. Primary objective of the study is to investigate the role of miRNA-210 in MR-ONJ pathogenesis. Secondary objective is to investigate the feasibility of salivary miRNAs detection as potential non invasive biomarkers for MR-ONJ. Methods: A case-and-control study has been designed. Inclusion criteria are malignant tumors with ongoing treatment or previous history of anti-resorptive or anti-angiogenic medications. The predictor variable is the presence or absence of MR-ONJ. Oral examination and dental x-ray examinations suggestive of MR-ONJ will be performed. Saliva sample will be collected according to the protocol by Navazesh. The methylation status of miRNA-210 will be investigated using quantitative PCR (Q-PCR). Values will be compared between case and control group. Results: The main expected results are to enroll approximatively twenty patients in case group and sixty patients in control group within a one year recruitment period, validate that saliva is a medium for the identification of biomarkers associated with MR-ONJ and confirm the hypothesis that miRNA-210 is involved in MR-ONJ development. Conclusions: Results acquired within this project would represent a contribute to the understanding of the molecular mechanisms driving to the development of MR-ONJ lesions. MiRNA-210 demethylation could serve as a potential therapeutic target for the treatment of MR-ONJ through stimulation of angiogenesis.

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