Optimization of Growth Conditions and Biological Activities of Nepalese Ganoderma lucidum Strain Philippine

Ganoderma lucidum has been extensively studied for its valuable medicinal importance. In this study, the artificial cultivation of G. lucidum strain Philippine in different culture media, including sawdust substrate, was performed and optimized on the Potato Dextrose Agar (PDA) media. Phytochemical, antibacterial, and antioxidant analyses were performed and compared between the ethanol extracts prepared from two different cultures (fruit from synthetic log culture and mycelia from PDA media culture). Both the 200 mg/mL and 100 mg/mL concentrations of extracts inhibited all the tested bacteria, and the results were promising than the corresponding control using antibiotics. The fruit extract showed higher antioxidant potential (150.6 ± 56.92 mg ascorbic acid equivalent/g extract) than mycelial extract (144.28 ± 81.72 mg ascorbic acid equivalent/g extract). The results indicate that fruiting bodies of G. lucidum cultivated in a complex dust medium possess higher antioxidant properties than mycelia culture, which can be further explored for therapeutic applications.

Pine Stands as Bioindicators: Justification for Air Toxicity Monitoring in an Industrial Metropolis

Five permanent sample plots (SPs; 200–250 trees per plot) were established in middle-aged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002–2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich’s model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye’s adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown.

Antibacterial Activity of Semi Purified Extract of Marine-Derived Trichoderma reesei PDSP 5.7 Using Bioguided Fractionation Method

Bioguided fractionation method is commonly used to obtained targeted fraction with certain biological activity. In this study, bioguided fractionation method using HPLC was applied to obtained antibacterial fractions from marine-derived fungus Trichoderma reesei PDSP 5.7. The result shows that fraction from mycelium and medium extract had antibacterial activity against ESBL E. coli and S. enterica ser. Typhi with a range of inhibition zone was 1.35±0.15 to 8.82±0.22 mm2. From 32 fractions of each extract, the mycelial extract had 7 active fractions, 3 active fractions from broth medium which extracted using 1-butanol and 5 active fractions from ethyl acetate extract. This study indicated that crude extract of fungus T. reesei PDSP 5.7 was more potential as the source of antibacterial agents rather than the crude extract that obtained from its broth medium.

An Extract Purified from the Mycelium of a Tomato Wilt-Controlling Strain of Fusarium sambucinum Can Protect Wheat against Fusarium and Common Root Rots

An approach to manage seed-transmitted Fusarium crown-foot-root rot (FCR, Fusarium spp.) and common root rot (CRR, Bipolaris sorokiniana) on wheat, avoiding environmental risks of chemicals, is seed treatments with microbial metabolites. F. sambucinum strain FS-94 that induces resistance to tomato wilt was shown by this study to be a source of non-fungitoxic wheat-protecting metabolites, which were contained in a mycelium extract purified by gel-chromatography and ultrafiltration. Plant-protecting effect of the purified mycelial extract (PME) was demonstrated in vegetation experiments using a rolled-towel assay and by small-plot field trials. To elucidate mechanisms putatively underlying PME protective activity, tests with cultured Triticum aestivum and T. kiharae cells, particularly the extracellular alkalinization assay, as well as gene expression analysis in germinated wheat seeds were used. Pre-inoculation treatments of seeds with PME significantly decreased the incidence (from 30 to 40%) and severity (from 37 to 50%) of root rots on seedlings without any inhibition of the seed germination and potentiation of deoxynivalenol (DON), DON monoacetylated derivatives and zearalenon production in FCR agents. In vegetation experiments, reductions in the DON production were observed with doses of 0.5 and 1 mg/mL of PME. Pre-sowing PME application on seeds of two spring wheat cultivars naturally infected with FCR and CRR provided the mitigation of both diseases under field conditions during four growing seasons (2013–2016). PME-induced ion exchange response in cultured wheat cells, their increased survivability, and up-regulated expression of some defensins’ genes in PME-exposed seedlings allow the suggestion of the plant-mediated character of disease-controlling effect observed in field.

Brefeldin-A: an Antiproliferative Metabolite of the Fungus Curvularia trifolii Collected from the Veracruz Coral Reef System, Mexico

In this paper the isolation and structure analysis of three sterols and brefeldin-A from the mycelial extract of the liquid fermentation of fungus <em>Curvularia trifolii</em> isolated from the marine sponge <em>Amphimedon compressa</em> collected from the Coral Reef System of Veracruz is reported. This is the first report on the production of brefeldin-A by the fungus <em>C. trifolii</em>. The results on isolation biological activity support the importance of the conservation of the Mexican ecosystems of coral reefs as a source of microorganisms with a pharmaceutical potential.

Systemic protection against pearl millet downy mildew disease induced by cell wall glucan elicitors from Trichoderma hamatum UOM 13

The obligate oomycete Sclerospora graminicola (Sacc.) Schroet, is the incitant of downy mildew disease, which is the main constraint in pearl millet production worldwide. Different elicitors from Trichoderma hamatum UOM 13, e.g. mycelial extract and cell wall glucans, were assessed for their resistance elicitation efficiency and the possible underlying mechanisms. Both mycelial extract and cell wall glucans of T. hamatum UOM 13 positively influenced seed quality parameters of pearl millet, significantly enhanced seed germination and seedling vigor in comparison to the untreated control. Seed priming with cell wall glucan elicitors of T. hamatum UOM 13 suppressed downy mildew on susceptible pearl millet seedlings under greenhouse conditions by induction of systemic host resistance. Of the different elicitor delivery methods tested, transplant root dip was more effective than seed treatment and foliar spray. A combination of transplant root dip + seed treatment + foliar spray was significantly more effective than the single delivery methods. The induced resistance corresponded to up regulation of genes of important defense proteins upon pathogen inoculation. Transcripts of genes of defense enzymes glucanase, phenylalanine ammonia lyase, peroxidase and polyphenoloxidase were significantly increased due to the T. hamatum UOM elicitor effect. Expression of hydroxyproline-rich glycoprotein genes, known to play an important role in cell wall cross-linking, were also up regulated in response to T. hamatum UOM cell wall glucan treatment. This study emphasizes the role of T. hamatum UOM as a potential elicitor of downy mildew resistance in pearl millet and presents novel insights into the involvement of important defense proteins mediating such as resistance trigger.