Fryderyk Chopin i Łesia Ukrainka. Per me

The key word of the essay is per me, as I present in it my own reminiscence from my childhood and youth, which was when I first came into contact with the works of two exceptional maestros: Frédéric Chopin and Lesya Ukrainka (the pen name of Larysa Kosach). Chopin created musical poetry, while Lesya – a rhythmic and melodic verbal poetry. The dialogue of the two authors sounds amazing, uncovering an optimistic apotheosis of survival and the trauma of loss – the states expressed in Chopin’s Marche funèbre and by Lesya Ukrainka in her poetry cycle devoted to this composition.The temporarily mature understanding of per me in both these creations also resulted from the trauma caused by the loss of close relatives (first, the grandad at a very young age) and hence became the drive for discovering the content of art and the means of expression characteristic to various branches of art.

Comparison of biological features of wild European rabbit mesenchymal stem cells derived from different tissues.

Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) has not been well studied. Here we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the species native region. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG­MSC). The six rMSC lines showed plastic adhesion with fibroblast-like morphology and were all shown to be positive for CD44 and CD29 expression (characteristic markers of MSCs), and negative for CD34 or CD45 expression. In terms of pluripotency features, all rMSC lines expressed NANOG, OCT4, and SOX2. Furthermore, all rMSC lines cultured under osteogenic, chondrogenic, and adipogenic conditions showed differentiation capacity. In conclusion, this study describes the isolation and characterization of new rabbit cell lines from different tissue origins, with a clear mesenchymal pattern. We show that rMSC do not exhibit differences in terms of morphological features, expression of the cell surface, and intracellular markers of pluripotency and in vitro differentiation capacities, attributable to their tissue of origin.

The Novel "Poor Folk" in the Context of Dostoevsky's Letters from the time of the Kuznetsk Events

The article shows the connection between the epistolary novel "Poor Folk", the events of the biography of F. M. Dostoevsky and his epistolary legacy of the Kuznetsk period (1855-1857). The novel "Poor Folk" created in after -penal colony time is considered as a high standard for the author, as a self-reflection. The letters of F. M. Dostoevsky from the time of the Kuznetsk events, where there are several excerpts about "Poor Folk", are presented as a workbook, a creative laboratory, where the writer develops a new quality of artistic expression characteristic of his mature works. For Dostoevsky, the novel "Poor Folk" is not just a brilliant debut that opened the way to "great literature", but also a text that has prophetic and life-creating modes, and has connections with the events of his life in Kuznetsk. Repeated reference to it, the presence of several versions of the novel, speaks about the peculiarities of the author's creative thinking and the significance of this work for the writer.

STYLISTIC DEVICES AS BASIC ELEMENTS OF ENRICHING ENGLISH LANGUAGE

The article deals with the study of stylistic techniques and means of expression characteristic of English speech expression, the study of means and methods of communication of independent sentences, as well as phrasal units, ways to combine them into fragments and whole speech texts. The paper identifies and generalizes scientific approaches to the study of stylistic syntax techniques, analyzes syntactic constructions that have expressive potential and pragmatic orientation. It is established that the subject of syntactic stylistics is also the study of the specifics of the functioning of expressive means and techniques in complex linguistic texts. It is found that the stylistic technique is realized, as a rule, in the context of phrasal units, paragraph and whole text, and the interaction of sentence models in these contexts gives emotional and expressive meaning to the segments of speech that they form. Thus, stylistic devices based on formal and semantic interactions of several syntactic constructions or sentences in a particular context, is one of the richest sources of speech expression. These stylistic devices serve as a means of poetic syntax, and are widely used in fiction, journalism and are found in scientific language. Despite the fact that each stylistic device individually contributes to the overall expressiveness of the text, a greater effect is achieved through their interaction, and with the help of these stylistic devices, contact with the addressee is established, the emotional background and emphaticity of the text are enhanced. Therefore, expressive means and stylistic techniques of syntax, which are created both with the use of stylistically marked constructions and without their participation, are one of the richest means of stylistics of the English language. The considered cases of violation of the usual logic of syntactic connection of parts of a complex sentence, semantic differences between their usual and occasional use bring subjective motives into the text and are used with certain stylistic purposes, which due to functioning together have a greater effect.

TRPM7-Mediated Calcium Transport in HAT-7 Ameloblasts

TRPM7 plays an important role in cellular Ca2+, Zn2+ and Mg2+ homeostasis. TRPM7 channels are abundantly expressed in ameloblasts and, in the absence of TRPM7, dental enamel is hypomineralized. The potential role of TRPM7 channels in Ca2+ transport during amelogenesis was investigated in the HAT-7 rat ameloblast cell line. The cells showed strong TRPM7 mRNA and protein expression. Characteristic TRPM7 transmembrane currents were observed, which increased in the absence of intracellular Mg2+ ([Mg2+]i), were reduced by elevated [Mg2+]i, and were inhibited by the TRPM7 inhibitors NS8593 and FTY720. Mibefradil evoked similar currents, which were suppressed by elevated [Mg2+]i, reducing extracellular pH stimulated transmembrane currents, which were inhibited by FTY720. Naltriben and mibefradil both evoked Ca2+ influx, which was further enhanced by the acidic intracellular conditions. The SOCE inhibitor BTP2 blocked Ca2+ entry induced by naltriben but not by mibefradil. Thus, in HAT-7 cells, TRPM7 may serves both as a potential modulator of Orai-dependent Ca2+ uptake and as an independent Ca2+ entry pathway sensitive to pH. Therefore, TRPM7 may contribute directly to transepithelial Ca2+ transport in amelogenesis.

Infiltration of Immunoinflammatory Cells and Related Chemokine/Interleukin Expression in Different Gastric Immune Microenvironments

Gastric mucosal immune microenvironment plays an important role in the occurrence and development of diseases such as inflammation and cancer. In the present study, single-sample gene set enrichment analysis (ssGSEA) was used to evaluate the expression of cytokines and the degree of immune cell infiltration in four different gastric mucosa tissues from normal gastric mucosa, simple gastritis, and atrophic gastritis to gastric cancer. Here, we show the immune microenvironments of these four gastric mucosae were significantly different. From inflammation to gastric cancer, most immunoinflammatory cells showed a downward trend such as central memory CD4 T cell. Instead, several cells showed an upward trend such as macrophage. Additionally, we found some chemokines/interleukins were illustrated to be low expressed (or highly expressed) in precancerous stage and highly expressed (or low expressed) in postcancerous stage, which demonstrated an opposite expression characteristic in pre-/postcancerous stage.

The (d)evolution of political communication in Italy: Beppe Grillo’s case

The article aims at analyzing the case of Beppe Grillo and his Five Stars Movement in terms of social, cultural and linguistic phenomenon that – initially as a virtual party without a structured organization – seems to conquer both right-wing and left-wing Italian citizens notwithstanding generational and ideological differences. The success of grillini (Grillo’s supporters) in the parliamentary election of 2018 as a consequence of Matteo Renzi’s constitutional referendum failure, represents a clear sign of the leadership crisis as well as the drifting apart of the ruling class that ignored the problems of ordinary people for several years. The analysis is focused on both form and content: on the one hand, the artistic expression characteristic of Grillo, his gestures, mimicry and direct language plenty of verbal hyperboles, rhetorical figures, swearwords and blasphemous obscenities that build his uncompromising charisma, on the other modern technologies and social media (including blogs, forums, profiles) that are used to communicate efficiently with the electorate, create an image of an open-minded politician keeping up with the outer world. All the aforementioned circumstances demonstrate an innovative approach based on political communication that devolves some level of decision-making power to the party supporters being active web users: bloggers and influencers at the same time. Such paradigm is followed by other representatives of the Italian political scene nowadays.

Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes

Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some macrophages persist in the absence of CSF1R. Here, we analyzed mpeg1:GFP–expressing macrophages in csf1r-deficient zebrafish and report that embryonic macrophages emerge followed by their developmental arrest. In larvae, mpeg1+ cell numbers then increased showing two distinct types in the skin: branched, putative Langerhans cells, and amoeboid cells. In contrast, although numbers also increased in csf1r-mutants, exclusively amoeboid mpeg1+ cells were present, which we showed by genetic lineage tracing to have a non-hematopoietic origin. They expressed macrophage-associated genes, but also showed decreased phagocytic gene expression and increased epithelial-associated gene expression, characteristic of metaphocytes, recently discovered ectoderm-derived cells. We further demonstrated that juvenile csf1r-deficient zebrafish exhibit systemic macrophage depletion. Thus, csf1r deficiency disrupts embryonic to adult macrophage development. Zebrafish deficient for csf1r are viable and permit analyzing the consequences of macrophage loss throughout life.

Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes

Macrophages derive from multiple sources of hematopoietic progenitors. Most macrophages require colony-stimulating factor 1 receptor (CSF1R), but some macrophages persist in the absence of CSF1R. Here, we analyzed mpeg1:GFP–expressing macrophages in csf1r-deficient zebrafish and report that embryonic macrophages emerge followed by their developmental arrest. In larvae, mpeg1+ cell numbers then increased showing two distinct types in the skin: branched, putative Langerhans cells, and amoeboid cells. In contrast, although numbers also increased in csf1r-mutants, exclusively amoeboid mpeg1+ cells were present, which we showed by genetic lineage tracing to have a non-hematopoietic origin. They expressed macrophage-associated genes, but also showed decreased phagocytic gene expression and increased epithelial-associated gene expression, characteristic of metaphocytes, recently discovered ectoderm-derived cells. We further demonstrated that juvenile csf1r-deficient zebrafish exhibit systemic macrophage depletion. Thus, Csf1r deficiency disrupts embryonic to adult macrophage development. Csf1r-deficient zebrafish are viable and permit analyzing the consequences of macrophage loss throughout life.