Uji Aktivitas Antikolesterol Ekstrak Daun Puring (Codiaeum variegatum (L.) Rumph. Ex. A.Juss) Secara In Vitro

AbstractCholesterol is an essential building material for the body to synthesize important substances such as cell membranes and insulation materials around nerve fiber as well as genital hormones and kidney of vitamin D and bile acids. Puring plants iclusive varieties of flowering plants who most of society interest because have varied colour leaf and have compound secondary metabolic substances of flavonoid, phenolic, triterpenoid, steroid and alkaloids. The purpose of this study was to determine the anticholesterol activity of puring leaves in vitro. The method used in vitro with Lieberman-Burchad reagent using a UV-Vis spectrophotometer measuring device at a wavelength of 665.0 nm. The concentration series used are 100; 200; 300; 400 and 500 ppm. The results showed that at a concentration of 500 ppm it can decrease cholesterol levels by 52.20% and The EC50 value obtained was 449.87 g/mL. The increase in each extract concentration showed an increase in the percent decrease in cholesterol levels.Keywords: cholesterol; puring leaf; lieberman-burchard AbstrakKolesterol merupakan bahan bangun esensial bagi tubuh untuk sintesis zat-zat penting, seperti membran sel dan bahan isolasi sekitar serat saraf, begitu pula hormon kelamin dan anak ginjal, vitamin D, serta asam empedu. Tanaman puring termasuk jenis tanaman hias yang banyak diminati masyarakat karena memiliki warna daun yang beragam dan memiliki kandungan senyawa metabolit sekunder berupa flavonoid, fenolik, triterpenoid, steroid dan alkaloid. Tujuan penelitian ini untuk mengetahui aktivitas antikolesterol pada daun puring secara in vitro. Metode yang digunakan secara in vitro dengan pereaksi Lieberman-Burchad menggunakan alat pengukur spektrofotometer UV-Vis pada panjang gelombang 665,0 nm. Seri konsentrasi yang digunakan yaitu 100; 200; 300; 400 dan 500 ppm. Hasil penelitian menunjukkan pada konsentrasi 500 ppm dapat menurun kadar kolesterol sebesar 52,20% dan nilai EC50 yang didapatkan sebesar 449,87 µg/mL. Peningkatan setiap konsentrasi ekstrak menunjukan peningkatan persen penurunan kadar kolesterol.Kata kunci: daun puring; kolesterol; Lieberman-burchard

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Cholesterol in pregnancy: a review of knowns and unknowns

Obstetric Medicine ◽

10.1258/om.2011.110003 ◽

2011 ◽

Vol 4 (4) ◽

pp. 147-151 ◽

Cited By ~ 27

Author(s):

Änne Bartels ◽

Keelin O'Donoghue

Keyword(s):

Cardiovascular Disease ◽

Vitamin D ◽

Fetal Development ◽

Plasma Cholesterol ◽

Adult Population ◽

The Body ◽

High Cholesterol ◽

Cholesterol Levels ◽

Growing Body ◽

In Pregnancy

Cholesterol forms part of every cell in the human body, and also helps make and metabolize hormones, bile acids and vitamin D. Human plasma cholesterol levels are determined by production in the liver and by dietary intake. Lipoproteins carry cholesterol around the body, and facilitate it crossing the placenta. Cholesterol is carefully monitored in the non-pregnant adult population, where its association with atherosclerosis and cardiovascular disease is well understood. Although it is known that cholesterol rises in pregnancy, at present it is not routinely measured or treated. The effects of maternal high cholesterol on pregnancy and on fetal development are not yet fully understood. However, a growing body of evidence from animal and human studies suggests adverse consequences of high cholesterol levels in pregnancy.

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AKTIVITAS ANTIKOLESTEROL EKSTRAK ETANOL BUAH KIWI HIJAU (Actinidia deliciosa)

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v6i2.344 ◽

2020 ◽

Vol 6 (2) ◽

pp. 183

Author(s):

Novena Yety Lindawati ◽

Desi Widya Ningsih

Keyword(s):

Quantitative Analysis ◽

Fatty Liver ◽

Operating Time ◽

Ethanol Extract ◽

The Body ◽

Actinidia Deliciosa ◽

Cholesterol Levels ◽

Ethanol Extracts ◽

Concentration Series ◽

Green Kiwifruit

Disease in the liver caused by hypercholesterolemia is fatty liver. Fatty liver is one of the damages to the liver, a condition in which the liver experiences fat accumulation. Green kiwifruit (Actinidia deliciosa) is a plant that contains a lot of flavonoid compounds can be used as cholesterol-lowering. Flavonoids are able to release cholesterol found in the walls of blood vessels and organs in the body. The purpose of this study was to determine the presence or absence of anticholesterol activity and EC50 values in ethanol extracts of green kiwifruit (Actinidia deliciosa) with a concentration series of 2.5; 5.0; 7.5; 10.0; 12.5 ppm. Ethanol extract of green kiwifruit was tested for active substance content and quantitative analysis using UV-Vis spectrophotometry method at a wavelength of 668 nm and operating time of 15 minutes. The results showed ethanol extract of green kiwifruit contains phenols, flavonoids, saponins, vitamin C which can reduce cholesterol levels with an average EC50 value of 7.3 ppm with a coefficient of variation value of 1.12%. Keywords : Cholesterol, Lieberman-burchard, Extract, Green kiwifruit (Actinidia deliciosa), UV-VIS spectrophotometry.

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Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering

Jurnal Kimia Sains dan Aplikasi ◽

10.14710/jksa.21.2.54-58 ◽

2018 ◽

Vol 21 (2) ◽

pp. 54-58 ◽

Cited By ~ 2

Author(s):

Devina Ingrid Anggraini ◽

Lily Fathrah Nabillah

Keyword(s):

Leaf Extract ◽

Ethanol Extract ◽

The Body ◽

Flavonoid Content ◽

Cholesterol Lowering ◽

Cholesterol Levels ◽

Method Of Extraction ◽

Lowering Activity ◽

Solvent Analysis

Cholesterol is a natural substance with physical characteristic similar to fat but has a steroidal group. The body requires cholesterol in normal amount; however, it will harm the body in excess amount. High cholesterol levels in the blood are dangerous because of the precipitation of cholesterol and other fatty substances resulting in atherosclerosis. Suji leaf (Dracaena angustifolia Roxb.) used as a natural dye has a high flavonoid content that is inferred to have cholesterol-lowering activity. This study aims to test the in vitro activity of suji leaf (Dracaena angustifolia Roxb.) extract in decreasing cholesterol level with various concentrations and to find the effective concentration (EC50). The method of extraction used was remaceration method with 70% ethanol solvent. Analysis of cholesterol-lowering activity was done by Lieberman-Burchard method by making variation of ethanol extract 400 ppm, 500 ppm, 600 ppm, 700 ppm, and 800 ppm. The results showed the percentage of cholesterol-lowering activity by 33.62%, 36.15%, 46.61%, 56.39% and 64.05% respectively. Value of EC50 activity of suji leaf extract is 632.50 ppm.

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Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells

Research Society and Development ◽

10.33448/rsd-v9i12.10810 ◽

2020 ◽

Vol 9 (12) ◽

pp. e8991210810

Author(s):

Andressa Rodrigues Lopes ◽

Vitor Gabriel Felipe ◽

Raquel Gouvêa dos Santos ◽

Wagner Gouvêa dos Santos

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Cell Line ◽

Pcr Amplification ◽

The Body ◽

Vdr Gene ◽

Vdr Polymorphism ◽

Vdr Polymorphisms ◽

Mcf 7

Vitamin D (VD) is a steroid hormone with multiple biological functions in the body and its activity requires the binding to the receptor named VDR. VDR polymorphisms seems to be involved in the development of several types of cancer. Herein we performed the genotyping of two VDR polymorphisms (Fok I and Taq I) in MCF-7 breast cancer and U87-MG glioblastoma (GBM) cell lines and investigated the antiproliferative effect of the VD analog cholecalciferol. Polymorphisms were identified by PCR-RFLP and the effect of VD was determined by viability and clonogenic assays. VD inhibited the growth of both tumor cells in vitro. MCF-7 cells were more sensitive than U87-MG cells at concentrations ranging from 0.1nM to 1000nM. The same primer pairs used for PCR amplification of VDR gene in MCF-7 failed to amplify a fragment of expected size in the U87-MG cell line. VDR Fok I and Taq I polymorphisms in breast cancer MCF-7 cells were characterized as FF (CC) and TT respectively. The absence of amplification of VDR gene fragment in U87-MG suggests a possible chromosomal rearrangement and/or impairment of gene expression of VDR which could interfere in the sensitivity of this cell line to vitamin D.

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Uji Aktivitas Antikolesterol Partisi N-Heksana, Metanol Dan Ekstrak Etanol Kulit Jeruk Nipis (Citrus Aurantiifolia) Secara In Vitro

Prosiding Seminar Nasional Kesehatan ◽

10.48144/prosiding.v1i.691 ◽

2021 ◽

Vol 1 ◽

pp. 403-412

Author(s):

Iesyi Lutfiyati ◽

Urmatul Waznah ◽

S Slamet ◽

W Wirasti

Keyword(s):

Ethanol Extract ◽

Test Sample ◽

Research Data ◽

The Body ◽

Cholesterol Levels ◽

Lime Peel ◽

Vitro Analysis ◽

Citrus Aurantiifolia ◽

In Vitro Analysis

AbstractCholesterol is an important sterol in human body tissue which belongs to the lipid group but cannot be hydrolyzed. Cholesterol has various uses in the body such as forming steroid hormones in the hormones estrogen and progesterone. However, if cholesterol levels in the blood are too high, it can cause blockage of blood flow which can lead to atherosclerosis. Lime contains secondary metabolites that function to reduce the increase in cholesterol levels in the blood. The purpose of this study was to determine the anticholesterol activity and to determine the EC₅₀ value of partition n-hexane, methanol, ethanol extract of lime peel (Citrus aurantiifolia) in vitro. Analysis of cholesterol activity is known by measuring cholesterol levels in vitro using Lieberman Burchard reagent. The analytical method used UV-Vis spectrophotometry at a wavelength of 665.0 nm with a series of test sample concentrations of 150 µg/ml; 300 µg/ml; 450 µg/ml; 600 µg/ml and 750 µg/ml. The research data shows that the decrease in cholesterol levels is directly proportional to the increase in the concentration in the sample. The EC₅₀ value of the n-hexane partition was 448.76 µg/ml; methanol partition as much as 448.98 µg/ml and ethanol extract as much as 450.18 µg/ml.Keywords: Anticholesterol; in vitro; lime peel; partition. AbstrakKolesterol merupakan sterol yang penting dalam jaringan tubuh manusia yang termasuk pada golongan lipid tetapi tidak dapat terhidrolisis. Kolesterol memiliki berbagai kegunaan dalam tubuh seperti pembentuk hormon-hormon steroid pada hormon esterogen dan progrsteron. Namun, jika kadar kolesterol dalam darah terlalu tinggi maka dapat menyebabkan penyumbatan aliran darah yang dapat mengakibatkan penyakit Aterosklerosis. Jeruk nipis memiliki kandungan metabolit sekunder yang berfungsi untuk mengurangi kenaikan kadar kolesterol dalam darah. Tujuan pada penelitian ini yaitu mengetahui aktivitas antikolesterol dan mengetahui nilai EC₅₀ dari partisi n-heksana, metanol, ekstrak etanol kulit jeruk nipis (Citrus aurantiifolia) secara in vitro. Analisis aktivitas kolesterol diketahui dengan mengukur kadar kolesterol secara in vitro menggunakan pereaksi Lieberman Burchard. Metode analisis menggunakan spektrofotometri UV-Vis pada panjang gelombang 665,0 nm dengan seri konsentrasi sampel uji 150 µg/ml; 300 µg/ml; 450 µg/ml; 600 µg/ml dan 750 µg/ml. Data penelitian menunjukkan penurunan kadar kolesterol berbanding lurus dengan peningkatan konsentrasi pada sampel. Nilai EC₅₀ partisi n-heksana sebanyak 448,76 µg/ml; partisi metanol sebanyak 448,98 µg/ml dan ekstrak etanol sebanyak 450,18 µg/ml. Kata kunci: Antikolesterol; in vitro; kulit jeruk nipis; partisi.

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Studi In Vitro: Efek Anti Kolesterol Ekstrak Daun Rambusa (Passiflora foetida L)

Jurnal Surya Medika ◽

10.33084/jsm.v4i2.606 ◽

2019 ◽

Vol 4 (2) ◽

pp. 60-65

Author(s):

Evi Mulyani

Keyword(s):

Leaf Extract ◽

Positive Control ◽

The Body ◽

Central Kalimantan ◽

Cholesterol Levels ◽

The People ◽

Measurement Results ◽

Passiflora Foetida

The Rambusa plant (Passiflora foetida L) is known to contain compounds for various treatments, empirically the people in Indonesia, especially Central Kalimantan, use the leaves of rambusa known as the cemot plant to reduce cholesterol levels in the blood. Chemically, cholesterol is a complex compound produced by the body with various functions, including producing sex hormones, adrenal cortex hormones, vitamin D, and the production of bile salts that help the intestines to absorb fat. So, if the dose is right or reasonable, the role of cholesterol in the body will be optimal. The purpose of this study was to determine the effect of anticholesterol in rambusa leaf extract. This study is an experimental study. The anticolesterol effect was tested by the modified enzymatic endpoint cholesterol method. The leaf extract of Rambusa was made with a concentration of 0.5 mg/ml, 1 mg/ml and 1.5 mg/ml as a positive control (multiplier) using simvastatin which was then reacted with reagent R1 and read the results using a photometer and UV-spectrophotometer. Vis with a wavelength of 546 nm. The measurement results using a photometer showed standard cholesterol levels (control negative) 200 mg/dl, positive control 195 mg/dl, 144 mg/dl (extract 0.5 mg/ml), 108 mg/dl (extract 1 mg/ml) and 103 mg/dl (extract 1.5 mg/ml). While the measurement of cholesterol absorbance and concentration using a UV-Vis spectrophotometer at a wavelength of 546 nm 0.179�159.8 mg/dl (Control positive), 0.123�109.8 mg/dl (extract 0.5 mg/ml), 0.110�98.2 mg/dl (extract 1 mg/ml), and 0.109�97.3 mg/dl (extract 1.5 mg/ml). As a conclusion, Rambusa leaf extract can reduce cholesterol levels in vitro.

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The Role of Polyphenols in the Modulation of Plasma Non-Enzymatic Antioxidant Capacity (NEAC)

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000116 ◽

2012 ◽

Vol 82 (3) ◽

pp. 228-232 ◽

Cited By ~ 7

Author(s):

Mauro Serafini ◽

Giuseppa Morabito

Keyword(s):

Antioxidant Capacity ◽

Dietary Intervention ◽

Ex Vivo ◽

The Body ◽

Dietary Polyphenols ◽

Enzymatic Antioxidant ◽

Endogenous Antioxidant

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.

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A Simple and Inexpensive Clinical Whole Body Counter

Nuklearmedizin ◽

10.1055/s-0038-1624972 ◽

1976 ◽

Vol 15 (05) ◽

pp. 248-253

Author(s):

A. K. Basu ◽

S. K. Guha ◽

B. N. Tandon ◽

M. M. Gupta ◽

M. ML. Rehani

Keyword(s):

The Body ◽

Whole Body ◽

Vitro Assay ◽

Body Counter ◽

Optimum Parameters ◽

Whole Body Counter ◽

Single Detector ◽

Background Index ◽

Iodide Crystal

SummaryThe conventional radioisotope scanner has been used as a whole body counter. The background index of the system is 10.9 counts per minute per ml of sodium iodide crystal. The sensitivity and derived sensitivity parameters have been evaluated and found to be suitable for clinical studies. The optimum parameters for a single detector at two positions above the lying subject have been obtained. It has been found that for the case of 131I measurement it is possible to assay a source located at any point in the body with coefficient of variation less than 5%. To add to the versatility, a fixed geometry for in-vitro counting of large samples has been obtained. The retention values obtained by the whole body counter have been found to correlate with those obtained by in-vitro assay of urine and stool after intravenous administration of 51Cr-albumin.

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