Welfare Assessment, End-Point Refinement and the Effects of Non-Aversive Handling in C57BL/6 Mice with Lewis Lung Cancer

Cancer-bearing mice are at risk of developing anxiety, pain, or malaise. These conditions may not only harm welfare but could also undermine data quality and translational validity in studies to develop therapeutic interventions. We aimed to establish whether, or at what point mice developing lung cancer show these symptoms, what measures can best detect their onset, and if data quality and animal welfare can be enhanced by using non-aversive handling (NAH). Welfare was monitored using various daily methods. At the beginning and end of the study, we also scored behaviour for general welfare evaluation, recorded nociceptive thresholds, and applied the mouse grimace scale (MGS). Cancer caused a decline in daily welfare parameters (body weight, and food and water consumption) beginning at around 4 days prior to euthanasia. As cancer progressed, rearing and walking declined to a greater extent in cancer-bearing versus control mice, while grooming, inactive periods, and MGS scores increased. A decline in nest building capability and food consumption provided a particularly effective means of detecting deteriorating welfare. These changes suggested a welfare problem arose as cancer developed, so similar studies would benefit from refinement, with mice being removed from the study at least 4 days earlier. However, the problem of highly varied tumour growth made it difficult to determine this time-point accurately. There were no detectable beneficial effects of NAH on either data quality or in terms of enhanced welfare.

Operations management on the front line of COVID-19 vaccination: building capability at scale via technology-enhanced learning

The globe is gripped by the COVID-19 pandemic. Mass population vaccination is seen as the solution. As vaccines become available, governments aim to deploy them as rapidly as possible. It is important, therefore, that the efficiency of vaccination processes is optimal.Operations management is concerned with improving processes and comprises systematic approaches such as Lean. Lean focuses explicitly on process efficiency through the elimination of non-value adding steps to optimise processes for those who use and depend on them.Technology-enhanced learning can be a strategy to build improvement capability at scale. A massive online programme to build capability in Lean has been developed by the regulator of England's National Health Service. Beta testing of this programme has been used by some test sites to refine their COVID-19 vaccination processes. The paper presents a case example of massive online learning supporting the use of Lean in the day-to-day operations management of COVID-19 vaccine processes.The case example illustrates the challenges that vaccination processes may present and the need for responsive and effective operations management. Building capability to respond rapidly and systematically in dynamic situations to optimise flow, safety and patient experience may be beneficial.Given the national imperative to achieve mass vaccination as rapidly as possible, systematic improvement methods such as Lean may have a contribution to make. Massive online programmes, such as that described here, may help with this effort by achieving timely knowledge transfer at large scale.

Application of modified starch in high-temperature-resistant colloidal gas aphron (CGA) drilling fluids

Colloidal gas aphrons (CGA) are finding increasing application in depleted oil and gas reservoirs because of their distinctive characteristics. To overcome the limitations of its application in high-temperature drilling, a modified starch foams stabilizer WST with a temperature resistance of 160 °C was synthesized via radical polymerization. The chemical structure of WST was characterized by Fourier infrared spectroscopy and results showed that all three monomers acrylamide, 2-acrylamido-2-methyl-1-propane sulfonic acid, and N-vinylpyrrolidone have been grafted onto starch efficiently. Based on the microscopic observations, highly stable aphrons have been successfully generated in the WST-based CGA drilling fluids within 160 °C, and most aphrons lie in the range of 10–150 μm. WST can provide higher viscosity at high temperatures compared to xanthan gum, which helps to extend foam life and stability by enhancing the film strength and slowing down the gravity drainage. Results show that WST-CGA aged at elevated temperatures (120–160 °C) is a high-performance drilling fluid with excellent shear-thinning behavior, cutting carrying capacity, and filtration control ability. The significant improvement of filtration control and well-building capability at high temperatures is an important advantage of WST-CGA, which can be attributed to the enhancement of mud cake quality by WST.

Impact of Gut Microbiome Manipulation in 5xFAD Mice on Alzheimer’s Disease-Like Pathology

The gut brain axis seems to modulate various psychiatric and neurological disorders such as Alzheimer’s disease (AD). Growing evidence has led to the assumption that the gut microbiome might contribute to or even present the nucleus of origin for these diseases. In this regard, modifiers of the microbial composition might provide attractive new therapeutics. Aim of our study was to elucidate the effect of a rigorously changed gut microbiome on pathological hallmarks of AD. 5xFAD model mice were treated by antibiotics or probiotics (L. acidophilus and L. rhamnosus) for 14 weeks. Pathogenesis was measured by nest building capability and plaque deposition. The gut microbiome was affected as expected: antibiotics significantly reduced viable commensals, while probiotics transiently increased Lactobacillaceae. Nesting score, however, was only improved in antibiotics-treated mice. These animals additionally displayed reduced plaque load in the hippocampus. While various physiological parameters were not affected, blood sugar was reduced and serum glucagon level significantly elevated in the antibiotics-treated animals together with a reduction in the receptor for advanced glycation end products RAGE—the inward transporter of Aβ peptides of the brain. Assumedly, the beneficial effect of the antibiotics was based on their anti-diabetic potential.

Exemplars revisited: Building primary teachers’ capability in assessment for learning

Building capability in assessment for learning is a goal of education systems internationally, though difficult for nations to successfully implement and sustain over time (Laveault & Allal, 2016). Evidence about the positive effects of assessment for learning practices on students’ learning and achievement is convincing, but implementation is made difficult by the realities for teachers to balance the multiple demands of pedagogy, curriculum and assessment, differentiated instruction, and time for professional learning

Taxonomy at a crossroads: Communicating value, building capability, and seizing opportunities for the future

I believe the top three questions facing taxonomy today all relate to the health and future of the discipline itself. 1. How do we ensure taxonomy is valued? 2. How do we build and maintain taxonomic capability? 3. How do we ensure taxonomy benefits from emerging opportunities?

Adjusting to working away and as an expatriate: learnings on what organisations can do to promote the mental health and well-being of workers and their families

Adjusting to frequent separations and reunions can put pressure on the relationships and families of those who work away. Although the work context is different, there are similar effects and challenges for workers, families and organisations across the military, expatriate and fly-in, fly-out (FIFO) research domains. Mental health, work performance, job satisfaction, relationships and parenting are all negatively affected by the extended periods of deployment or posting and the regular and ongoing shorter periods of FIFO work. At the individual level, personality dimensions (emotional stability, sociability, openness to new experiences), locus of control, intelligence, self-sufficiency and cultural intelligence have been shown to significantly affect these impacts and provide organisations with starting points for both the screening of candidates for roles and coaching them to better adjust and cope cross-culturally. The recruitment and onboarding processes should be underpinned by the principles of managing expectations and building capability, and this means that realistic and relevant information should focus on the realities of the work, the work environment and host country. The onboarding process that seeks to socialise the worker into the organisation and the culture should assume the worker will take at least 6 months to settle into the role. Predeparture training should engage with the worker and their families to ensure the development of coping skills and practical strategies for managing communication, parenting and relationships. Effectively managing the psychosocial risks faced by workers across these domains will improve the mental health and well-being of workers and their families.