Regulation of Swine Growth by Backfat Tissue during Growing and Finishing Stages

Recently, interest in the function of pig backfat (BF) has increased in the field of livestock animals, and many transcriptome-based studies using commercial pig breeds have been conducted. However, there is a lack of comprehensive studies regarding the biological mechanisms of Korean native pigs (KNPs) and Yorkshire pig crossbreeds. In this study, therefore, BF samples of F1 crossbreeds of KNPs and Yorkshire pigs were investigated to identify differentially expressed genes (DEGs) and their related terms using RNA-sequencing analysis. DEG analysis identified 611 DEGs, of which 182 were up-regulated and 429 were down-regulated. Lipid metabolism was identified in the up-regulated genes, whereas growth and maturation-related terminologies were identified in the down-regulated genes. LEP and ACTC1 were identified as highly connected core genes during functional gene network analysis. Fat tissue was observed to affect lipid metabolism and organ development due to hormonal changes driven by transcriptional alteration. This study provides a comprehensive understanding of BF contribution to crossbreeds of KNPs and Yorkshire pigs during growth periods.

Phenotypic characterization of native pig in Romblon province, Philippines

This study was conducted to determine the phenotypic characteristics of native pigs in the province of Romblon. Specifically, it identifies the phenotypic characteristics, morphometric diversity, and effective population size of the native pigs. This study used the descriptive design. The data gathered were statistically analysed using frequency/percentage, mean, regression analysis and correlation. From the analysis and interpretation conducted it was found out that: majority of the snout were long and thin; the head profile was straight; most of the ear type was droopy; the ear orientation were projected forward; have straight hair; the coat color pattern were plain; the coat color was black; the skin were smooth; the tail were straight and the backline was straight except for gilt which is swaybacked. The Shannon Weiner Diversity Index on the phenotypic characteristics of native pigs in the province of Romblon was observed evenly distributed. There is significant relationship observe on the morphometric diversity of native pigs.

Multi-Probiotic Lactobacillus Supplementation Improves Liver Function and Reduces Cholesterol Levels in Jeju Native Pigs

We evaluated the dietary effects of multiple probiotics in Jeju native pigs, using basal diet and multi-probiotic Lactobacillus (basal diet with 1% multi-probiotics) treatments (n = 9 each) for 3 months. We analyzed growth performance, feed efficiency, backfat thickness, blood parameters, hematological profiles, adipokines, and immune-related cytokines in pig tissues. Average daily gain, feed intake, feed efficiency, backfat thickness, and body weight were not significantly different between both groups. In Lactobacillus group, total protein (p < 0.08) and bilirubin (p < 0.03) concentrations increased; blood urea nitrogen (p < 0.08), alkaline phosphatase (p < 0.08), and gamma-glutamyltransferase (p < 0.08) activities decreased. Lactobacillus group showed decreased adiponectin (p < 0.05), chemerin (p < 0.05), and visfatin expression in adipose tissues, and increased TLR4 (p < 0.05), MYD88 (p < 0.05), TNF-α (p < 0.001), and IFN-γ (p < 0.001) expression in the liver. Additionally, NOD1 (p < 0.05), NOD2 (p < 0.01), and MYD88 (p < 0.05) mRNA levels in proximal colon tissue upregulated significantly. Colon, longissimus dorsi muscle, fat tissue, and liver histological analyses revealed no significant differences between the groups. Conclusively, Lactobacillus supplementation improved liver function and reduced cholesterol levels. Its application may treat metabolic liver disorders, especially cholesterol-related disorders.

Insights on the historical biogeography of Philippine native pigs and its relationship with Continental domestic and wild boars

The Philippine archipelago was believed to have never been connected to the Asian continent, even during the severe Quaternary sea-level drops. As a result, the history of pig dispersal in the Philippines remains controversial and must have some anthropogenic origin associated with some human migration events. In this study, the context of origin, dispersal, and the level of genetic introgression in Philippine native pigs were deduced using mitochondrial DNA D-loop analysis altogether with domestic pigs and wild boars corresponding to their geographic origin. Results revealed a considerable genetic diversity (0.900±0.016), and a widespread Asian pig-ancestry (94.60%) were revealed in the phylogenetic analysis with admixed European pig-origin (5.10%) harboring various fractions of ancestry from Berkshire and Landrace. The close genetic connection between the continental wild boars and domestic pigs present in the Philippine pigs corroborates our hypothesis of a genetic signal that could potentially be associated with the recently reported multiple waves of human migrations to the Philippines during the last 50,000 years. The high frequency of haplotypes (54.08%) that collapsed in the D7 haplogroup represent an interesting challenge as its distribution does not coincide with the hypothesized migratory route of the Neolithic Austronesian-speaking populations. We detected the first Pacific Clade signature and ubiquitously distributed D2 haplotypes which postulate the legitimate dispersal of pigs associated with the multiple waves of human migrations involving the Philippines. The multimodal mismatch and neutrality test statistics both Fu’s Fs and Tajima’s D correlates the long stationary period of effective population size revealed in the Bayesian skyline plot. While the sudden decrease in population was consistent with the pronounced population bottleneck of Asian and European pigs during the interglacial periods of the Pleistocene.

Morphological Characterization of Native Pigs (Sus scrofa domesticus L.) in Bohol Province, Philippines

This study assessed the morphological characteristics of the native pig population in Bohol Island, Philippines. A total of 100 sexually-mature native pigs (80% gilt/sow and 20% barrow/boar) were selected from the municipalities of Balilihan, Bilar, Talibon, Ubay, Loon, Guindulman, Mabini, San Miguel, Pilar, and Alburquerque, which are densely populated with native pigs (based on the data of the Office of the Provincial Veterinarian-Bohol). Purposive sampling was used to select the experimental pigs based on the phenotypic traits that qualify them as native. Likewise, remote barangays where native pigs are most likely raised were selected as sample sites. The qualitative (hair color, color pattern, and ear orientation) and quantitative (tail length, teat number, estimated body weight using body length, and heart girth measurements) traits were recorded based on subjective observation and actual measurements, respectively. Qualitatively, the native pigs in Bohol have predominantly (73%) black hair. In terms of color pattern, plain color (91%), spotted (4%), and patchy (5%) were noted. The native pigs also revealed droopy (54%), slightly droopy (37%), and erect (9%) ear orientations. Meanwhile, quantitative traits revealed an overall body length of 38.07 ± 8.75 inches, heart girth of 36.9 ± 8.83 inches, body weight of 67.29 ± 41.15 kg, tail length of 9.78 ± 2.45 inches, and 12.38 ± 1.33number of teats. Significant differences across sampling areas(p<0.05)and sex (p<0.01) were observed. Generally, females had higher values than males, and there was a significant difference (p<0.05) in trait values among age groups.

PSIII-11 Effect of Breast cancer metastasis-suppressor 1-like (BRMS1L) genotype on teat number traits in Jeju native pigs (JNP) and Landrace

The purpose of this study was to compare the changes of teat number traits of Jeju native pig (JNP) and Landrace varieties according to the breast cancer metastasis-suppressor 1-like (BRMS1L) genotype. The number of teat varies according to the single-base polymorphism with a G or A base 1,087 from the start codon ATG in the exon 1 region of the BRMS1L gene. The total teat number was examined at birth for 28 JNPs and 72 Landraces, and the BRMS1L genotype was analyzed by Pyrosequencing. As a result, the genotyping frequency of JNP was 1 A / A type, 9 A / G type, and 18 G / G type, whereas, on the contrary, Landrace had 70 A / A type, 2 A / C type, and C / C was identified as 0 heads. The total teat number was between the two varieties was 13.32 ± 0.95 and 14.51 ± 1.03, respectively (P &lt; 0.001). According to genotypes in Jeju native pig breeds, the total number of nipples was 15 A / A, 14.0 ± 0.82 A / G, and 12.89 ± 0.74 G / G (P &lt; 0.018). Conversely, Landrace was 14.54 ± 1.02 A / A and 13.5 ± 0.5 A / G. As a result of the above study, the frequency of varieties and genotypes was found to have a significant effect on the difference in nipple count. Based on the results of this study, if the improvement of Jeju native pigs is to be carried out, it is expected that the number of nipples will increase and the mammalian capacity will increase.

The role of animal breeding with special regard to native pigs of food supply and rural development in Laos

The present paper explored the influence of breeding native pigs on livelihood conditions and the contribution of native pigs to the rural development and rural poverty reduction in the rural areas of Lao PDR. Pig production plays an important role in meat supply for both urban and rural areas of Laos. It is clear that most of the pig products in the country come from smallholder pig farms, and more than 90 percent of those products are the native pigs mostly raised by farmers in remote areas. In general, livestock production distributed between 15–18 percent to GDP, while most of animal production still remains as the traditional methods. Rural development is always the first priority of the Laos government since its independence in 1975, however, the poverty rate in rural areas remained high at 23% in 2018. It might block the development goal of the government which will lead the country out of the least development status by 2020. The food security and malnutrition in the rural or mountainous areas are considered as the majority issue that both government and several international organizations have been thriving hard to overcome, which researchers showed that more than 45% of children under 5 years of age were stunted, and 28% of them were underweight. Inspired of more than 50 % of the households in the rural areas of Laos reported they consumed chicken and pork at least one day a week. While native pigs play an important role on meat supply, it also constituted around 9–14 % of annual income of the households in rural areas. Therefore, the increase the production of pigs and poultry is one option to promote the meat supply to households in the rural areas of Laos. This paper will be a pathway to guide and identify for the final decision to what experiment will be implemented on Lao native pig in Laos (2021–2023) to complete the comparative study on reproductive physiology and reproductive management methods of Hungarian and Lao Indigenous pig breed. Which found it still needs further afford to research and improve more about native pig performance for all areas of productive and quality management.

Genetic Diversity and Ancestral Study for Korean Native Pigs Using 60K SNP Chip

The Korean native pig (KNP; Sus scrofa coreanus) is an indigenous porcine breed in South Korea considered as a valuable but dwindling genetic resource. Studies using diverse methodologies and genetic markers suggest that this population originated from the Manchu province of Northeastern China and migrated approximately 3000 years ago into the Korean peninsula. This study aimed to verify those findings by performing diversity and ancestral analyses using the 60K single-nucleotide polymorphism (SNP) BeadChip on 891 pigs of 47 breeds worldwide. We also performed principal component analysis (PCA), ancestry analyses, phylogenetic tree analysis using SNPhylo, and linkage disequilibrium analysis. Furthermore, we generated heatmap, obtained Nei’s genetic distance and FST values, and explored the heterozygosity of commercial and native Korean pigs. The results demonstrated that KNP pigs are more closely related to European breeds than to Chinese breeds. In addition, as previous studies have suggested, our admixture analyses indicated that KNP pigs showed distinguishable genetic structure.