Genetic diversity within leukemia-associated immunophenotype-defined subclones in AML

Acute myeloid leukemia (AML) is a highly heterogeneous disease showing dynamic clonal evolution patterns over time. Various subclones may be present simultaneously and subclones may show a different expansion pattern and respond differently to applied therapies. It is already clear that immunophenotyping and genetic analyses may yield overlapping, but also complementary information. Detailed information on the genetic make-up of immunophenotypically defined subclones is however scarce. We performed error-corrected sequencing for 27 myeloid leukemia driver genes in 86, FACS-sorted immunophenotypically characterized normal and aberrant subfractions in 10 AML patients. We identified three main scenarios. In the first group of patients, the two techniques were equally well characterizing the malignancy. In the second group, most of the isolated populations did not express aberrant immunophenotypes but still harbored several genetic aberrancies, indicating that the information obtained only by immunophenotyping would be incomplete. Vice versa, one patient was identified in which genetic mutations were found only in a small fraction of the immunophenotypically defined malignant populations, indicating that the genetic analysis gave an incomplete picture of the disease. We conclude that currently, characterization of leukemic cells in AML by molecular and immunophenotypic techniques is complementary, and infer that both techniques should be used in parallel in order to obtain the most complete view on the disease.

Imaging Markers of Vascular Brain Health: Quantification, Clinical Implications, and Future Directions

Cerebrovascular disease (CVD) manifests through a broad spectrum of mechanisms that negatively impact brain and cognitive health. Oftentimes, CVD changes (excluding acute stroke) are insufficiently considered in aging and dementia studies which can lead to an incomplete picture of the etiologies contributing to the burden of cognitive impairment. Our goal with this focused review is 3-fold. First, we provide a research update on the current magnetic resonance imaging methods that can measure CVD lesions as well as early CVD-related brain injury specifically related to small vessel disease. Second, we discuss the clinical implications and relevance of these CVD imaging markers for cognitive decline, incident dementia, and disease progression in Alzheimer disease, and Alzheimer-related dementias. Finally, we present our perspective on the outlook and challenges that remain in the field. With the increased research interest in this area, we believe that reliable CVD imaging biomarkers for aging and dementia studies are on the horizon.

LEGAL CLAIM OF A MILITARIZED FORMATION OFFICER FOR ADMISSION TO SERVICE

Employment in militarized services should be of interest not only to representatives of the doctrine of administrative law, but also to labor law. One-sided approach to these issues (only from the perspective of one field of law) gives an incomplete picture and forces to use certain simplifications. The article deals with the issue of the possibility of a militarized service officer claiming to be admitted to service. The analyzed issue was presented in a comparative legal aspect. The aim of the article is to answer the question whether, in the event of an officer not admitting to the service, he has a claim similar to the employee’s claim for admission to work.

Ecstasies

Embodiment in general and sexuality especially are often marginalized in the study of religion, particularly when the object of study is a mainstream tradition practiced by dominant groups in global North / global West cultures. Yet, despite the centrality of belief and the marginalization of practice in the lives of many who currently identify as spiritual, too close a focus on belief and identity to the neglect or exclusion of embodiment and practice risks forming an incomplete picture of spirituality as a phenomenon today. One site in which spirituality is explicitly constructed—even first realized—through embodiment is in the practice of spiritual BDSM (bondage/discipline, dominance/submission, and sado-masochism). This chapter follows Fennell’s work on sacred kink by arguing for the importance of queer leather spirituality to spirituality studies as a whole.

Sources for Research on Childhood and Childhood History in Poland in the 19th and Early 20th Century – Selected Problems

The aim of the present article is to draw attention to the informative value of selected types of source materials which may provide a basis for research on childhood and childhood in the nineteenth and early twentieth centuries. At the same time, an attempt will be made to indicate the sorts of research problems which can be analyzed using these sources. Some types of sources will be discussed in more depth, with emphasis on those that are particularly rich and valuable in terms of information about children and childhood, but have not yet been fully exploited by researchers in historical and pedagogical analyses, have been used only occasionally, or have even been ignored altogether. The informative potential of the source materials for the study of history of children and childhood will be presented using the examples of such written sources as women’s, family and children’s press, diaries and memoirs, children’s literature, guidebooks, as well as iconographic sources. Comparing and combining sources, as well as considering them in a complementary manner may contribute to reconstructing the as yet incomplete picture of childhood and children in the Polish historiography of the nineteenth and early twentieth centuries.

Introducing Regular Turnover Details, 1960–2015: A dataset on world leaders’ legal removal from office

The premier data on leader survival focus on the violent, dramatic means by which leaders ‘exit’ office. This information, vital for many research questions, constitutes a valuable public good for the community. Yet, it provides an incomplete picture of the political rise and fall of world leaders. The burgeoning study of leaders using survival analysis requires a fine-grained understanding of not just when, but why and how leaders lose power. We cannot, for example, conclude that a leader’s exit implies a successful application of international pressure if her removal stems from pre-set constitutional laws and the immediate successor has long been considered the heir apparent. The Regular Turnover Details dataset remedies this problem. Two principal variables report information about the manner of each leader’s exit and the relationship between outgoing and incoming leaders, allowing analysts to arbitrate between exits that suggest political failure and those that don’t, identify non-political leaders (such as interim and technocratic executives), and determine whether leaders constitute heirs to power or challengers thereto.

Nitecap: An Exploratory Circadian Analysis Web Application

Circadian omics analyses present investigators with large amounts of data to consider and many choices for methods of analysis. Visualization is crucial as rhythmicity can take many forms and p-values offer an incomplete picture. Yet statically viewing the entirety of high-throughput datasets is impractical, and there is often limited ability to assess the impact of choices, such as significance threshold cutoffs. Nitecap provides an intuitive and unified web-based solution to these problems. Through highly responsive visualizations, Nitecap enables investigators to see dataset-wide behavior. It supports deep analyses, including comparisons of two conditions. Moreover, it focuses upon ease-of-use and enables collaboration through dataset sharing. As an application, we investigated cross talk between peripheral clocks in adipose and liver tissues and determined that adipocyte clock disruption does not substantially modulate the transcriptional rhythmicity of liver but does advance the phase of core clock gene Bmal1 (Arntl) expression in the liver. Nitecap is available at nitecap.org and is free-to-use.

Distinguishing intergroup and long-distance relationships

While intergroup relationships (IRs) dominate the literature on human sociality, long-distance relationships (LDRs) are also highly prevalent in human social life; however, they are often conflated with IRs or overlooked entirely. We suggest that by focusing on IRs to the exclusion of LDRs, scholars are painting an incomplete picture of human sociality. Though both IRs and LDRs function to provide resource access, LDRs likely evolved before IRs in the human lineage and are especially effective for both responding to widespread resource shortfalls and providing access to resources not locally available. To illustrate the importance of distinguishing IRs from LDRs, we draw on an example from rural Bolivia. This case study illustrates how (1) IRs and LDRs vary in importance, even between nearby communities, due to differences in socioecology and past experience, and (2) researcher expectations about IR prevalence can bias both data collection and data interpretation. We close by highlighting areas of LDR research that will expand our understanding of human sociality.

Temporary Employment, Permanent Stigma? Perceptions of Temporary Agency Workers Across Low- and High-Skilled Jobs

Research on temporary agency work emphasizes that temporary agency workers (TAWs), particularly those in low-skilled jobs associated with precariousness and low social prestige, are likely to be exposed to poor treatment, as well as stigmatization. On the contrary, stigmatization of TAWs in high-skilled jobs has not been treated in much detail in previous studies. Literature provides an incomplete picture of stigmatization within the broader field of temporary employment regarding the focus on low-skilled jobs. Hence, the present qualitative study is based on data from interviews of a heterogeneous sample of TAWs employed in low- and high-skilled jobs in Germany. By using and modifying Boyce and colleagues’ (2007) model of stigmatization, the study shows that stigmatizing treatment towards TAWs occurs across all skill levels, although the intensity and form of those experiences, as well as coping strategies, differ. Thereby, this study contributes to a more differentiated and skill level-specific understanding of how TAWs perceive and cope with stigmatization linked to their employment status. It also provides an important opportunity to advance Boyce and colleagues’ (2007) complex model of TAW stigmatization with empirical underpinnings.

Do Epigenetic Timers Control Petal Development?

Epigenetic modifications include histone modifications and DNA methylation; such modifications can induce heritable changes in gene expression by altering DNA accessibility and chromatin structure. A number of studies have demonstrated that epigenetic factors regulate plant developmental timing in response to environmental changes. However, we still have an incomplete picture of how epigenetic factors can regulate developmental events such as organogenesis. The small number of cell types and the relatively simple developmental progression required to form the Arabidopsis petal makes it a good model to investigate the molecular mechanisms driving plant organogenesis. In this minireview, we summarize recent studies demonstrating the epigenetic control of gene expression during various developmental transitions, and how such regulatory mechanisms can potentially act in petal growth and differentiation.