Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery

Since tumor-draining pulmonary vein blood (PV) is enriched in tumor-secreted products, we hypothesized that it would also be enriched in tumor-derived exosomes, which would be important in the metastasis process. We characterized exosomes from PV of 61 resected non-small cell lung cancer (NSCLC) patients to evaluate its potential as relapse biomarkers. Exosomes were characterized using transmission electron microscopy, western blot and nanoparticle tracking analysis and we examined time to relapse (TTR) and overall survival (OS). Differences between PV and peripheral vein were found. PV was enriched in smaller exosomes than the paired peripheral vein (p = 0.01). Moreover, PV exosome size mode was able to identify relapsed patients (Area under the curve [AUC] = 0.781; 95%CI: 0.6641–0.8978), in whom exosome size was smaller (<112 nm; p < 0.001). The combination of PV exosome size and N (lymph node involvement) showed an AUC of 0.89 (95%CI: 0.80–0.97). Moreover, smaller PV exosome size was associated with shorter TTR (28.3 months vs. not reached, p < 0.001) and OS (43.9 months vs. not reached, p = 0.009). Multivariate analyses identified PV exosome size and stage as independent prognostic markers for TTR and OS. PV exosome size is a promising relapse biomarker after surgery that can add valuable information to clinical variables.

Size Distribution and Growth of young Payangka Fish, Ophieleotris aporos (Bleeker) from Lake Tondano

The research was conducted at the location of Lake Tondano, Minahasa District.The young payangka fish, locally known as nike fish by fishermen of the area. This study aims to determine the size distribution, length-weight relationships and to know the captivity growth in the aquarium. The research is expected to be a source of information about the fish of young payangka (Nike) and be able to contribute on the efforts of nike fish management in Lake Tondano for the future. Sampling is done by using a triangular shaped trap (sibusibu) with a length of 4m, width of 1.30m (top), and a mesh size of 0.5mm. The length frequency distribution with the size mode appeared most during the first of observation was in the range of 13.5 to 15.4 class size and changed in size in the third week of the 19.5-21.4 class sizes. Later in the fifth week the mode is in the range of 15.5-17.4 class size and changes in size in the seventh week at the range of 23.5-25.4 class size. The length-weight relationship of the study was showed by allometric growth (b = 2.70; b <3) which means the length increases faster than weight gain. Absolute weight growth of 0.2532g was recorded and absolute length growth of 10.12mm for 12 weeks was observed as well. Growth of a relatively large weight gained with value of 0.067mg occurs between the seventh week and the eighth week.Keywords : Nike Fish, growth, Tondano ABSTRAKPenelitian ini dilaksanakan di lokasi Danau Tondano Kabupaten Minahasa. Ikan payangka muda dikenal dengan sebutan ikan nike oleh masyarakat lokal. Penelitian ini bertujuan untuk mengetahui distribusi ukuran, hubungan panjang-berat dan untuk mengetahui pertumbuhan yang di pelihara di akuarium. Manfaat penelitian yaitu diharapkan dari penelitian ini dapat menjadi sumber informasi mengenai ikan payangka serta payangka muda (Nike) dan mampu memberikan kontribusi bagi upaya pengelolaan ikan nike di Danau Tondano di masa yang akan datang. Pengambilan sampel dilakukan dengan menggunakan alat tangkap serok (sibusibu) yang berbentuk segitiga dan memiliki ukuran panjang 4m, lebar 1,30m (bagian atas), dan ukuran mata jaring 0,5mm. Distribusi frekuensi ukuran panjang dengan modus atau ukuran yang paling banyak muncul pada minggu pertama terdapat pada kelas ukuran 13,5-15,4 dan mengalami perubahan ukuran pada minggu ketiga pada kelas ukuran 19,5-21,4. Selanjutnya pada minggu kelima modusnya ada pada kelas ukuran 15,5-17,4 dan mengalami perubahan ukuran pada minggu ketujuh di kelas ukuran 23,5-25,4. Hubungan panjang dan berat dari penelitian didapatkan hasil pertumbuhan alometrik (b=2,70), b<3 yang artinya pertambahan panjang lebih cepat dari pertambahan berat. Pertumbuhan berat mutlak sebesar 0,2532g dan pertumbuhan panjang mutlak sebesar 10,12mm selama 12 minggu. Pertumbuhan berat yang relative besar nilainya yaitu 0.067mg terjadi antara minggu ketujuh dengan minggu kedelapan.Kata kunci : Ikan Nike, Pertumbuhan, Tondano

A Class of Methods with Optimal Stability Properties for the Numerical Solution of IVPs: Construction and Implementation

Considering the methods with future points technique from second derivative general linear methods (SGLMs) point of view, makes it possible to improve their stability properties. In this paper, we extend the stability regions of a modified version of E2BD formulas to optimal one and show its effectiveness by numerical verifications. Also, implementation issues, with numerical experiments, of these methods are investigated in a variable step-size mode.

Abundance and distribution of lantern fishes (Myctophiformes: Myctophidae) around San Pedro Martir Island, Gulf of California, during 2008

Myctophids (Myctophidae) are a group of abundant mesopelagic fishes in the world´s oceans and are known as the main feeding resource for several high trophic level predators. Changes in abundance may be related to population size of some commercially important species that feed on them. Only two of the myctophid species reported for the Gulf of California were found in the present study: Benthosema panamense and Triphoturus mexicanus. The highest abundance and biomass of myctophids were found during the warm season (June and September), with B. panamense being the most abundant species (20,954 ind 1000 m-3), as well as the one with highest biomass (17,165.8 g 1000 m-3). B. panamese had a size mode interval of 35-40 mm, while T. mexicanus presented a size mode interval of 40-45 mm; both species had negative allometric growth. During the temperate season (February and April) B. panamense was distributed in the northwest, west, and southern regions around the island, while T. mexicanus was found in the north, west, and southern regions. During the warm season B. panamense was found distributed around the entire island and T. mexicanus was found in the west, south, and east regions of the island. These species are common around San Pedro Martir Island, with the highest values of abundance and biomass occurring during summer upwelling's.

SEBARAN UKURAN PANJANG DAN NISBAH KELAMIN IKAN MADIDIHANG (Thunnus albacares) DI SAMUDERA HINDIA BAGIAN TIMUR

Ikan madidihang atau tuna sirip kuning (Thunnus albacares) merupakan salah satu komoditas penting bagi industri perikanan di Indonesia dengan hasil tangkapan tertinggi dibandingkan jenis tuna lainnya. Sebagai dasar pengelolaan sumberdaya ikan yang berkelanjutan, diperlukan data dan informasi tentang komposisi ukuran layak tangkap yaitu membandingkan proporsi rata-rata ikan tertangkap (Lc) dan matang gonad (Lm), serta nisbah kelamin sebagai indikator pendugaan kemampuan memijah. Pengumpulan data dilakukanmelalui program observasi diatas kapal rawai tuna yang berbasis di Benoa, Pelabuhanratu dan Bungus dari bulan Agustus 2005 hingga November 2013. Penghitungan nisbah kelamin menggunakan uji Chi-Square (X2) dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa distribusi ukuran panjang cagak ikan madidihang berkisar antara 30-179 cm, modus ukuran 106-110 cm dan rata-rata 101,65 cm. Sebanyak 81,03% madidihang yang tertangkap berukuran lebih besar daripada Lm yang berarti telah layak tangkap. Nisbah kelamin betina:jantan adalah 1:1,45 mengindikasikan dominansi ikan jantan. Hubungan antara nisbah kelamin dengan panjang ikan menunjukkan signifikansi dimana ikan betina semakin berkurang pada ukuran 120-180 cm, serta tidak ditemukan lagi pada ukuran lebih dari 170 cm. Korelasi nisbah kelamin dan panjang cagak dapat dideskripsikan dengan persamaan regresi sebagai berikut: 1,8013 - 0,0099 FL dengan nilai R2=0,8058.Yellowfin tuna or YFT (Thunnus albacares) is one of the important commodity for the fishing industry in Indonesia because it has the highest catches compared with other tunas. In order to fisheries resources management, it was necessary to monitor the size composition compared between proportion average size captured (Lc) and maturity size (Lm) to meets the size eligibility, as well as the sex ratio as an indicator to estimate the ability of spawn. Data collected by scientific observers program which was following tuna longline operation mainly based in Benoa, Palabuhanratu and Bungus Fishing Port, from August 2005 to November 2013. Chi-Square analysis with 95%confidence level also implemented to determine sex ratio between female and male. The result indicated that YFT were caught has size ranged between 30-179 cm, size mode ranged between 106-110 cm and the mean was 101,65 cm.Mostly YFT (81,03%) was greater than its maturity size (Lm) and that’s mean have been worthy to be captured. Sex ratio of (F:M) 1:1,45 was observed which indicates male was dominant. Correlation between sex ratio and length proved to be significant where the female was diminishing in size between 120-180 cm, even female was no longer found in size more than 170 cm. Correlation between sex ratio and length can described as a regression equation=1,8013 - 0,0099 FL; R2=0,8058.

Pottery Vessel Function and Foodways at El Chorro de Maíta, Cuba

Our approach assumes that pots are tools, containers whose performance characteristics are adjusted to their primary uses. Traditional agricultural peoples generally distinguish among multiple vessel shapes that have distinct intended uses. In this article, we present afunctional analysis of vessel shapes and sizes performed on a sample of 160 rim sherds from the site of El Chorro de Malta, Cuba. These were assigned to 13 defined vessel shapes, most of which displayed more than one size mode based on estimated orifice diameters. The majority of specimens from El Chorro de Malta are low-profile, composite-contour bowls made in medium and large size modes, followed in frequency by simple-contour bowls and plates. Late Ceramic Age peoples in the Greater Antilles are historically documented as participating in the manioc breadcake-cassareep- stewpot foodway common to much of the tropical lowlands of northern South America. Consequently, it should be possible to show how the container assemblage of El Chorro de Malta is adapted to the requirements of that foodway. We note that, ethnographically, the elaborate processing of bitter manioc itself to produce breadcakes as a staple food does not necessarily require pottery vessels at all. Nonetheless, common stewing as a key component of the foodway—including the production of the condiment called cassareep in the Guianas—can require several containers with potentially distinct performance requirements: one to collect the juice below the sleeve press, another to reduce the expressed juice to the thickened sauce over afire, and a third, the stewpot itself, adapted to simmering vegetable and meat stews. We provisionally suggest that some of the most common shape-size classes at El Chorro de Malta are suited to producing and serving stews and cassareep, the traditional complement to eating manioc breadcake.

Arctic low-level boundary layer clouds: in situ measurements and simulations of mono- and bimodal supercooled droplet size distributions at the top layer of liquid phase clouds

Aircraft borne optical in situ size distribution measurements were performed within Arctic boundary layer clouds with a special emphasis on the cloud top layer during the VERtical Distribution of Ice in Arctic clouds (VERDI) campaign in April and May 2012. An instrumented Basler BT-67 research aircraft operated out of Inuvik over the Mackenzie River delta and the Beaufort Sea in the Northwest Territories of Canada. Besides the cloud particle and hydrometeor size spectrometers the aircraft was equipped with instrumentation for aerosol, radiation and other parameters. Inside the cloud, droplet size distributions with monomodal shapes were observed for predominantly liquid-phase Arctic stratocumulus. With increasing altitude inside the cloud the droplet mean diameters grew from 10 to 20 μm. In the upper transition zone (i.e., adjacent to the cloud-free air aloft) changes from monomodal to bimodal droplet size distributions (Mode 1 with 20 μm and Mode 2 with 10 μm diameter) were observed. It is shown that droplets of both modes co-exist in the same (small) air volume and the bimodal shape of the measured size distributions cannot be explained as an observational artifact caused by accumulating data point populations from different air volumes. The formation of the second size mode can be explained by (a) entrainment and activation/condensation of fresh aerosol particles, or (b) by differential evaporation processes occurring with cloud droplets engulfed in different eddies. Activation of entrained particles seemed a viable possibility as a layer of dry Arctic enhanced background aerosol (which was detected directly above the stratus cloud) might form a second mode of small cloud droplets. However, theoretical considerations and model calculations (adopting direct numerical simulation, DNS) revealed that, instead, turbulent mixing and evaporation of larger droplets are the most likely reasons for the formation of the second droplet size mode in the uppermost region of the clouds.

Arctic low-level boundary layer clouds: in-situ measurements and simulations of mono- and bimodal supercooled droplet size distributions at the cloud top layer

Aircraft borne optical in-situ size distribution measurements were performed within Arctic boundary layer clouds, with a special emphasis on the cloud top layer, during the VERtical Distribution of Ice in Arctic Clouds (VERDI) campaign. The observations were carried out within a joint research activity of seven German institutes to investigate Arctic boundary layer-, mixed-phase clouds in April and May 2012. An instrumented Basler BT-67 research aircraft operated out of Inuvik over the Mackenzie River delta and the Beaufort Sea in the Northwest Territories of Canada. Besides the cloud particle and hydrometeor size spectrometers the aircraft was equipped with instrumentation for aerosol, radiation and other parameters. Inside the cloud, droplet size distributions with monomodal shapes were observed for predominantly liquid-phase Arctic stratocumulus. With increasing altitude inside the cloud the droplet mean diameters grew from 10 μm to 20 μm. In the upper transition zone (i.e. adjacent to the cloud-free air aloft) changes from monomodal to bimodal droplet size distributions were observed. It is shown that droplets of both modes co-exist in the same (small) air volume and the bimodal shape of the measured size distributions cannot be explained as an observational artifact caused by accumulating two droplet populations from different air volumes. The formation of a second size mode can be explained by (a) entrainment and activation/condensation of fresh aerosol particles, or (b) by differential evaporation processes occurring with cloud droplets engulfed in different eddies. Activation of entrained particles seemed a viable possibility as a layer of dry Arctic enhanced background aerosol was detected directly above the stratus cloud might form a second mode of small cloud droplets. However, theoretical considerations and a model simulation revealed that, instead, turbulent mixing and evaporation of larger droplets most likely are the main reasons for the formation of the second droplet size mode in the uppermost region of the clouds.