Kandungan klorofil dan karotenoid Kacang Tunggak (Vigna unguiculata (L.) Walp.) pada umur tanaman yang berbeda

Chlorophyll is a pigment of photosynthesis which is also efficacious as a cure for brain, lung, and oral cancer, and can be used as a disinfectant, antibiotic and dietary supplement, while carotenoids are useful as antioxidants. The content of the two pigments is strongly influenced by the age of the plant, especially for vegetable crops used in leaves such as cowpea (Vigna unguiculata (L.) Walp.). The purpose of this research is to know the pattern of chlorophyll content and carotenoid of cowpea leaf during the growth phase and to know the age of the right plant for harvesting cowpea so that the highest chlorophyll and carotenoid content is obtained. Cowpea seeds obtained from seeds are added then planted in pots containing ready-made planting media in the greenhouse. Chlorophyll and carotenoid analyzes were performed at different plant ages from 1 to 11 weeks. The research design used is Completely Randomized Design. Data were analyzed using variance analysis and continued with Duncan test at 95% significance level. The results showed that the highest chlorophyll and carotenoid content was produced by the cowpea leaf in the vegetative phase or before the flowering time. The highest chlorophyll content was obtained at 4WAP (weeks after planting) of age crop, while the highest carotenoid content was obtained in 3WAP.

Eksplorasi benih Jati Putih (Gmelina arborea Roxb) dari berbagai variasi habitat untuk populasi pemuliaan

White teak (Gmelina arborea Roxb) is one of the types developed for the development of plantations, this species is an exotic tree that grows quickly, the planting technique is not difficult and has a good economic value. The wood is used for various purposes, especially for the manufacture of construction materials, carpentry, plywood, matches, containers and other wood craft materials. One aspect that determines the success of plantation development is the availability of seeds, especially quality seeds that are superior genetically, physically and physiologically, available in sufficient quantities, on time and have the ability to adapt to the environment in which they grow. The development of this plant needs to be accompanied by a breeding program, one of the forest plant breeding activities is exploration. Exploration activities of a type of plant in its natural habitat are important to support research and development activities of an institution and to meet market needs for these seeds. The purpose of seed exploration is to get white Teak seeds that represent a variety of habitats to support breeding activities. Exploration of white teak seeds is carried out in Kalianda (South Lampung), Bondowoso (East Java), Bantul (Yogyakarta), Parung Panjang (West Java) and Lombok (West Nusa Tenggara). The results of the exploration obtained 108 mother trees from Kalianda 25 mother trees with seed size (18.32 mm; 10.25 mm), Bondowoso 30 mother trees (17.78 mm; 9.64 mm), Bantul 21 mother trees (19, 24 mm; 11.40 mm), Parung length 15 mother trees (19.41 mm; 9.42 mm) and Lombok 17 mother trees (18.58 mm; 10, 31 mm).

Pengembangan klon Sukun (Artocarpus altilis (Park.) Fosberg.) unggulan untuk mendukung ketahanan pangan

Breadfruit (Artocarpus altilis) is one of multipurpose tree species that their wood, bark, fruit and leaves can be utilized for human life. The main product of breadfruit is its fruit as one of the potential food sources that has a fairly high nutrient content and is widely consumed as an additional food and even traditional staple foods such as in some countries the Pacific region. The challenge in developing breadfruit as an alternative food source for the community is to prepare the plant material of superior breadfruit clones which have high fruit production and have enough nutrients and chemicals as a source of food and medicinal ingredients. Based on the clones selection at the clonal test plantation in Gunungkidul, Yogyakarta obtained 5 excellent breadfruit populations representing breadfruit cultivars in Indonesia, namely Cilacap, Yogyakarta, Bali island, Bone and Manokwari. To ensure the availability of seedlings of superior breadfruit clones, the development of planting stocks production techniques is carried out by root cuttings, shoot cuttings and stem cuttings in the nursery that showed better growth succeess (70-100%). Furthermore, information dissemination is carried out to increase efforts to develop breadfruit by the community / user. Breadfruit planting activities are carried out to increase the availability of food reserve forests in the future, both independently and collaboratively with stakeholders who interested in developing of breadfruit plantations.

Isolasi dan identifikasi bakteri asam laktat dari pangan fermentasi berbasis ikan (Inasua) yang diperjualbelikan di Maluku-Indonesia

Lactic acid bacteria are beneficial bacteria for the health of the body by improving the balance of the intestinal microflora. Lactic acid bacteria can generally be isolated from fermented foods, fruits, vegetables and meat. Inasua is a traditional fermented fish product originating from Central Maluku (Teon, Nila and Serua islands) wherein the fermentation process is carried out at room temperature for a certain time. The purpose of this study was isolation and identification based on the morphological characteristics of lactic acid bacteria in inasua. Isolation of Lactic Acid Bacteria was carried out using the pour plate method and the streak method on the media de Man, Rogosa and Sharpe Agar (MRSA) and Nutrient Agar media. The culture was incubated at 370C for 48 hours. The growing colonies were observed for morphological characteristics and GRAM staining of bacteria was carried out. The results of isolation of BAL from inasua in the media de Man, Ragosa and Sharpe Agar (MRSA) + CaCO3 obtained 4 bacterial isolates, namely INS-A1, INS-A2, INS-A3 and INS-A4 and only 2 isolates that have the characteristics of positive GRAM with Bacillus forms, namely INS-A2 and INS-A4. It is suspected that the 2 isolates were isolates of Lactic Acid Bacteria. The results of isolation on Nutrient Agar (NA) media contained 5 isolates namely INS-B1, INS-B2, INS B3, INS-B4, INS-B5 which have characteristics as negative GRAM shaped Bacillus

Aplikasi biomaterial aktif dari daging ikan gabus (Channa striata) untuk penyembuhan luka pascaoperasi pada hewan model tikus Wistar

One problem for postoperative patients is tissue recovery takes a long time due to the occurrence of blood clotting disorders and immune system. Snakehead fish was known to contain bioactive ingredients that can potentially speed up the process of tissue repair as a result of injuries. Snakehead fish meat contains a lot of minerals, albumin, and amino acids needed for tissue protein synthesis and increases the antioxidant glutathione. This study was conducted to test the extraction of snakehead fish flesh against skin tissue wound healing in Wistar rats. The study was conducted with a completely randomized design consisting of three treatments with 6 replication, which include (P0) a negative control (without treatment madecassol and snakehead fish meat extract), (P1) madecassol commercial drug treatment, and (P2) treatment of snakehead fish meat extract. Each material was injected intramuscularly in Wistar rats at a dose of 9 ml/kg bb for 7 days. Data analysis uses Anova followed by test Honestly Significant Difference (HSD) with a significance of 95%. Treatment of snakehead meat extract with a dose of 9 ml/kg bb a significant effect on skin tissue repair after injury, better than treatment with madecassol and control with the average value of extensive tissue remaining after recovering from the condition of the wound by 0.2 cm2; 0.33 cm2; and 0.4 cm2, respectively. Snakehead fish meat extract with a dose of 9 ml/kg bb injected intramuscularly can accelerate the recovery of tissue in the skin after the wound in Wistar rats.

Regenerasi Jamuju (Dacrycarpus imbricatus (Blume.) de Laub.) di Cagar Alam Gebugan, Kabupaten Semarang Jawa Tengah

Gebugan Nature Reserve is one of the protected forest that still has the natural habitat of Dacrycarpus imbricatus. D. imbricatus has the potential of producing wood that is widely used by the people while the exploitation of Jamuju causes this tree species difficult to find in their natural habitat. The aim of this research was to asses D. imbricatus regeneration. The method used for taking jamuju was systematic method. The number of plots used were 15 with 10 m x 10 m area for each plot. The number of individual jamuju at the tree level, pole level, sapling level and seedling level were counted at each plot. The result of this research showed the regeneration of jamuju in Gebugan Nature Reserve was poor, indicated by tree-level individual density (5 individuals/0.15 ha = 33.3 ind/ha), pole level (not found), sapling (4 individuals/0.15 ha = 26.6 ind/ha), and seedlings (21 individuals/0.15 ha = 140 ind/ha). The environmental factors of Jamuju were found to grow at the altitude of 1,050 m asl, temperature 26.7°C to 27°C, humidity 53% to 61%, soil pH 6.3 to 7.