Aplikasi biomaterial aktif dari daging ikan gabus (Channa striata) untuk penyembuhan luka pascaoperasi pada hewan model tikus Wistar

2018 ◽  
Vol 1 (2) ◽  
pp. 13
Author(s):  
Sunarno Sunarno ◽  
Rahayu Damayanti ◽  
Alfisa Devi ◽  
Muhammad Fikri ◽  
F Pratiwi ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Wistar Rats ◽  
Negative Control ◽  
Skin Tissue ◽  
Meat Extract ◽  
Average Value ◽  
Fish Meat ◽  
Fish Flesh ◽  
Significant Difference ◽  
Tissue Recovery

One problem for postoperative patients is tissue recovery takes a long time due to the occurrence of blood clotting disorders and immune system. Snakehead fish was known to contain bioactive ingredients that can potentially speed up the process of tissue repair as a result of injuries. Snakehead fish meat contains a lot of minerals, albumin, and amino acids needed for tissue protein synthesis and increases the antioxidant glutathione. This study was conducted to test the extraction of snakehead fish flesh against skin tissue wound healing in Wistar rats. The study was conducted with a completely randomized design consisting of three treatments with 6 replication, which include (P0) a negative control (without treatment madecassol and snakehead fish meat extract), (P1) madecassol commercial drug treatment, and (P2) treatment of snakehead fish meat extract. Each material was injected intramuscularly in Wistar rats at a dose of  9 ml/kg bb for 7 days. Data analysis uses Anova followed by test Honestly Significant Difference (HSD) with a significance of 95%. Treatment of snakehead meat extract with a dose of 9 ml/kg bb a significant effect on skin tissue repair after injury, better than treatment with madecassol and control with the average value of extensive tissue remaining after recovering from the condition of the wound by 0.2 cm2; 0.33 cm2; and 0.4 cm2, respectively. Snakehead fish meat extract with a dose of 9 ml/kg bb injected intramuscularly can accelerate the recovery of tissue in the skin after the wound in Wistar rats. 

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Effect Of Epigallocatechin-3-Gallate (EGCG) On The Number Of Macrophage Cells In Inflammation Of Pulp With Mechanical Injury

2020 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
Kun Ismiyatin ◽  
Devi Eka Juniarti ◽  
Djoko Agus Purwanto ◽  
Adecha Ekalipta Primazafira
Keyword(s):  
Nitric Oxide ◽  
Tissue Repair ◽  
Wistar Rats ◽  
Antioxidant Properties ◽  
Recovery Process ◽  
Mechanical Injury ◽  
Mechanical Trauma ◽  
Macrophage Cells ◽  
Pulp Inflammation ◽  
Significant Difference

Background: Pulpitis is an inflammatory pulp that can occur due to mechanical trauma that causes perforation of the pulp. Treatment of pulpitis Emergency frequently using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the recovery process. An alternative consideration with Epigallocatechin 3-gallate (EGCG) which has good antioxidant properties and increases the complexity of inflammation by inhibiting the production of inducible nitric oxide synthase (iNOS) and nitric oxide so that levels migration of inflammatory cells to the area of injury will decrease and inflammation will occur shorter then initiates the proliferation so the recovery process and tissue repair will be more rapidly occurring. One of the cells that support tissue repair is macrophages. Purpose: this study aims to explain the effect of EGCG on the number of macrophage cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats teeth divided into four groups, namely control (C), and 3 treatment groups T1, T2, and T3. Each group consisted of 6 rats prepared, then T1 was named EGCG hydrogel 60 ppm, T2 was given EGCG hydrogel 90 ppm, while T3 was given EGCG hydrogel 120 ppm. On the 3rd day, Wistar rats were decapitated to continue making HPA preparations. Results:This study showed a significant difference in each group (p< 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm  is effectivein increasing the number of macrophage cells. 

scholarly journals The effect of meniran herbs extract (Phyllantus niruri) to triglycerides blood level in wistar rats

2012 ◽  
Vol 10 (1) ◽  
pp. 23-27
Author(s):  
JUDO YUSTANTO KAHONO ◽  
KISRINI KISRINI ◽  
YUL MARIYAH
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Total Period ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Kahono JY, Kisrini, Mariyah Y. 2012. The effect of meniran herbs (Phyllantus niruri) extract to blood triglyceride level in rats. Biofarmasi 10: 23-27. The aim of this experiment was to determine the effect of meniran herbs (Phyllantus niruri L.) extract to blood triglycerides level in Wistar rats (Rattus norvegicus). This experiment was a laboratory experiment with pre and post-test with a control group design. The samples were 30 male Wistar rats with ±200 gram of body weight and ±2 months old. The samples were divided into five groups by using a purposive random sampling technique and each group consisted of six rats. Group I as a negative control, group II as a positive control, and groups III, IV and V as meniran herbs extract groups with first dose, second dose, and third dose. All rats were simultaneously given with hyperlipemic feed. Group II was simultaneously administered with Cholvastin 0.5 mg/200 grams body weight/day. Groups III, IV and V were simultaneously treated with meniran herbs extract at dose of 25 mg, 50 mg and 100 mg/200 grams body weight/day. The total period of this experiment was 21 days. At the day 1st and 21st, blood was collected from rat orbital venous plexus. Serum was analyzed for their pre and post-test blood triglycerides. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by a post-hoc test. The value of p=0.002 (p<0.05) showed there was a significant difference between pre-test and post-test in delta means of blood triglycerides level among groups. Meniran herbs extract had a significant triglycerides lowering effect against blood triglycerides level of Wistar rats. The most potential of meniran herbs extract dose was 100 mg/200 gram body weight/day.

scholarly journals Effect of Orthosiphon stamineus Benth on Hematologic Parameters in Lead Acetat Induced Wistar Rats

2021 ◽  
Vol 15 (7) ◽  
pp. 2048-2050
Author(s):  
DWI Retnoningrum ◽  
Nyoman Suci Widyastiti ◽  
Ariosta .
Keyword(s):  
Platelet Count ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Negative Impact ◽  
Wistar Rat ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Orthosiphon Stamineus ◽  
Significant Difference

Introduction: Lead exposure has a negative impact on health. Lead can caused abnormality hematologic parameters. Orthosiphon stamineus Benth is one of the herbal medicines and known to have antioxidant, anti-inflammatory, and anti-angiogenesis. Objective: This study aims to prove the effect of Orthosiphon stamineus Benth on hematologic parameters in lead acetate induced Wistar rat. Method: The study used posttest only control group design. The samples were 30 male Wistar rats, divided into 5 groups. Normal control group (NC) was given normal saline. Negative control group (NG) was induced by lead acetate 30 mg/kg/day. GT-50, GT-100, GT-200 was induced by lead acetate and orally administered by 50,100, and 200 mg/kg Orthosiphon extract once daily for 14 days respectively. One-way Anova and post hoc LSD were used for statistical analysis. Result: Hemoglobin level and leukocyte count in all group were not different (p=0.56 and p=0.25). There was a significant difference in platelet count between all group (p=0.035). Platelet count in NC group (900 ±171 x103/μl) was significantly higher than NG group (656±201 x103/μl, p=0.016), NG group was significantly lower than GT-50 (869±134 x103/μl, p=0.032), GT-100 (930±162 x103/μl, p=0.008) and GT-200 (938±148 x103/μl, p=0.006) Conclusion: There is a decrement of platelets number in lead acetate exposure. Orthosiphon stamineus Benth may increase platelet counts in lead acetate induced Wistar rat. Keywords: Orthosiphon stamineus Benth, hemoglobin, leukocytes, platelets

scholarly journals Difference in Level of Malondialdehyde, Total Cholesterol, and Triglyceride After Administration of Passion Fruit Seed’s Ethanol Extract in Wistar Rats

2019 ◽  
Author(s):  
Dina Keumala Sari ◽  
Marianne Marianne ◽  
Sri Lestari ◽  
Lidya Imelda Laksmi
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Negative Control ◽  
Passion Fruit ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Preclinical Trial ◽  
Significant Difference

High oxidative stress in cells due to inflammation process or excessive cell proliferation would produce oxidants or free radicals with biomarkers, one of which is malondialdehyde (MDA). Passion fruit seed&rsquo;s contain high antioxidant and are expected to decrease the level of cholesterol and MDA. The objective is to identify the effect of passion fruit seed&rsquo;s ethanol extract in Wistar rats that have been fed with atherogenic feed. The method was preclinical trial (post-test control group design) in rats, by administering passion fruit seed&rsquo;s ethanol extract for 14 days. This study used 26 male rats aged two months, divided into 5 groups. The result showed significant difference in MDA level which was found in group that was given passion fruit seed extract 10mg/kg BW with positive control group that was given standard feed. Passion fruit seed&rsquo;s extract showed significant difference in level of triglyceride, which was found in negative control group that was given atherogenic feed with group that was given passion fruit seed&rsquo;s extract 5mg/kg BW (mean&plusmn;standard deviation: 1.09&plusmn;0.30 mg/dL vs 0.77&plusmn;0.25mg/dL; p=0.048). This study showed that passion fruit seed&rsquo;s ethanol extract had significant lowering effect in level of MDA, total cholesterol, and triglyceride for 14 days.

scholarly journals Pilot Study for Azoxymethane-induced Colon Cancer in Male Wistar Rats

Medicinus ◽  
2020 ◽  
Vol 7 (5) ◽  
pp. 120
Author(s):  
Riska Taufik ◽  
Abidin Pandianta ◽  
Rinaldy Kusuma ◽  
Imelda L. Winoto ◽  
Ivet Suriapranata ◽  
...  
Keyword(s):  
Pilot Study ◽  
Wistar Rats ◽  
Colonic Mucosa ◽  
Negative Control ◽  
Aberrant Crypt Foci ◽  
Chemical Agent ◽  
Significant Difference ◽  
Alternative Agent ◽  
Male Wistar Rats ◽  
Gross Examination

<div class="WordSection1"><p><strong>Introduction </strong><strong>:</strong> The use of animal models could significantly further the elucidation of Colorectal Cancer (CRC) molecular pathogenesis and help in the discovery of preventive and therapeutic agents for the disease. Dimethylhydrazine (DMH) is a widely used chemical agent for Carcinogen induced CRC model. As agent, DMH is however becoming less readily available; hence in this Pilot Study we use Azoxymethane (AOM), a DMH metabolite as an alternative agent to induce CRC in male Wistar rats.</p><p> </p><p><strong>Methods:</strong> Forty two male Wistar rats at six weeks of age were randomly assigned into negative control groups and groups receiving two AOM injections subcutaneously (SC) within one week interval at 15 mg/kg body weight (BW) and 20 mg/kg BW respectively. Rats were sacrificed 8, 16 and 24 weeks post- AOM administration. Aberrant crypt foci (ACF) were analyzed. Tumor foci were characterized by gross examination and histopathological characteristics.</p><p> </p><p><strong>Results:</strong> All rats in the AOM groups developed tumors in the colonic mucosa. Formation of ACF was detected starting from 8 weeks post-AOM injection. The highest number of ACF with multiple crypts was observed at 16 weeks post-AOM administration. The total number of ACF did not vary between the two AOM doses. Mild, moderate and severe dysplastic cells were observed in colonic mucosa starting 8 weeks post-AOM injection. There was no statistically significant difference between number of severe dysplastic cells between the two AOM doses.</p><p> </p><p><strong>Conclusion:</strong> Administration of AOM 15 mg/kg BW SC is able to induce CRC in male Wistar rats. Higher dose is not necessary since it does not result in higher tumor incidence. This cancer model may be utilized to study chemopreventive effect of various agents in the future.</p></div>

scholarly journals Aktivitas Membran Bakiko (Bayam-Kitosan-Kolagen) terhadap Ekspresi Matriks Metaloproteinase-9 pada Luka Bakar Derajat II

2020 ◽  
Vol 7 (1) ◽  
pp. 58
Author(s):  
Hazmi Dwinanda Nurqistan ◽  
Al Munawir ◽  
Ika Rahmawati Sutejo
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Negative Control ◽  
Tissue Growth ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Collagen Membrane ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference

Matrix metalloproteinase-9 (MMP-9) plays an important role in regulating the degradation and deposition extracellular matrix (ECM) that are important for wound reepithelization. However, excessive protease activity can lead to delayed wound healing. Kaemferol in spinach can inhibit the activity of MMP-9. Chitosan and collagen can help tissue growth and have an antimicrobial effect. Chitosan-collagen membranes have been widely used for wound healing but, the results are unsatisfactory. Therefore, the researcher wanted to know the activity of bakiko (Spinach-Chitosan-Collagen) membrane to MMP-9 expression on second degree burn with immunohistochemical method. This research was true-experimental laboratories design with post test only control group design. The experimental animals used were 36 white wistar rats (Rattus novergicus) male, divided into 4 groups ie control group, negative control, positive control (bioplacenton), and treatment (bakiko membrane). Data were obtained by calculating MMP-9 using ImageJ software. The analysis using anova test showed that p value less than 0.05 indicated a significant difference. This study showed that there was a decrease in MMP-9 activity by bakiko membrane in grade II burn with immunohistochemical method in wistar rats.

scholarly journals The Use of Torbangun Leaf Powder (Coleus amboinicus L) in Increasing Prolactin in Breastfeedings Wistar Rats and Bodyweight of the Litters

2021 ◽  
Vol 3 (1) ◽  
pp. 26-33
Author(s):  
Stella T Hasianna ◽  
July Ivone ◽  
Sifa D Shinta ◽  
R Zahra Nadhira
Keyword(s):  
Body Weight ◽  
Breast Milk ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
Breast Milk Production ◽  
Significant Difference ◽  
Leaf Powder ◽  
Coleus Amboinicus

Research on local herbs that have the effect of increasing breast milk production isneeded to help mothers with insufficient breastmilk. This research was conducted to determinethe effect of torbangun (Coleus amboinicus L) leaf powder in increasing prolactin inbreastfeeding Wistar rats and the bodyweight of the litters. Thirty breastfeeding Wistar rats weredivided into 5 groups with 5 litters each. The treatments given were 108 mg, 216 mg, and 432 mgtorbangun leaf powder (TLP), with domperidone as the positive control, and CMC 10% as thenegative control. The treatment was given 3 times/day for 14 days. Tukey's HSD means testshowed a significant difference in prolactin levels between the negative control (NC), TLP1, andTLP2 groups and the TLP3 and positive control (PC) groups with all of them having p = 0.000.There was no difference in the increase in body weight of litters between treatment groups (p =0.866). In conclusion, torbangun leaves increase the prolactin levels in breastfeeding rats buthave no difference in the increase in body weight of litters between treatment groups. Keywords: torbangun leaves; prolactin; bodyweight; breastfeeding

scholarly journals Comparison of The Number of Macrophage in Full Thickness Wound Incision between Merremia mammosa Extract Treatment and NaCl in Male Wistar Rats

1970 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Fajar Kurniawan Hidayat ◽  
Ulfa Elfiah ◽  
Kristianningrum Dian Sofiana
Keyword(s):  
Diabetes Mellitus ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Field Of View ◽  
Control Group ◽  
Full Thickness ◽  
Significant Difference ◽  
Male Wistar Rats

The incidence of diabetes mellitus in Indonesia continues to increase. There are some complications in the diabetic condition, one of which is diabetic ulcer. Diabetic ulcers in diabetes patient can increase the risk of amputation and expensive treatment costs, so the alternative treatmeant such as Merremia mammosa wich has antiinflamatory and antidiabetic is needed. This study aimed to determine the comparison of the number of macrophage in the incisional wound in hyperglycemic male wistar rats between treatment with Merremia mammosa extract and NaCl. The in vivo test was done by creating wound incision on the mice backs and treated with gentamycin ointment 5% in a positive control group, NaCl in a negative control group and Merremia mammosa extract in a dose of 100m), 200mg, 400mg in treatment group. The result obtained by counting the number of macrophage in histopatholgy examination. The result showed the number of macrophage were 0,36 cells/field of view in a negative control group, 0,52 cells/field of view in a positive control group, 0,48 cells/field of view in all Merremia mammosa treatment groups. The data analysis showed no significant difference with p-value of 0,729. In conclusion, there was no significant difference between the used of Merremia mammosa extract and NaCl on full thickness incisional wounds of hyperglycemic male wistar rats. Keywords: Diabetes Mellitus, Merremia mammosa extract, incisional wound

scholarly journals Toxicity Studies of Striga hermonthica (Delile) Benth Leaf Extract in Rats

2020 ◽  
Vol 2 (2) ◽  
pp. 7-18
Author(s):  
M. M. Onakpa
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Striga Hermonthica ◽  
Oral Toxicity ◽  
Toxicity Studies ◽  
Group I ◽  
Significant Difference ◽  
Male Wistar Rats

Striga hermonthica is a ubiquitous hemi-parasitic plant commonly known as witch-weed and is used in West Africa as a traditional herbal medicine for the treatment of an array of diseases. In this study, methanol leaf extract of S. hermonthica was used to investigate the acute and sub-acute toxicity effects in male Wistar rats. In the acute toxicity studies, Wistar rats were divided into six groups comprising of negative control and extract treated groups (250, 500, 1000, 1500 and 2000 mg/kg of extract orally). The rats were observed for 72 hours while in the sub-acute oral toxicity studies, the rats were divided into 4 groups consisting of 5 rats per group. The extract was administered orally at doses of 25, 50 and 100 mg/kg daily for 28 days to groups II, III and IV respectively while group I (negative control) received 2 ml of distilled water. The dose of 2000 mg/kg did not cause any mortality or signs of toxicity in the treated rats during the acute and subacute toxicity studies did not show any treatment-related abnormalities in the hematological (RBC, Hb, WBC, Lymphocytes) and biochemical (AST, ALT, ALP, TB, Glucose, HDL, LDL, Total protein, Albumin) parameters while the liver revealed lesions in the histopathology studies, there were no treatment-related lesions observed in the heart, lungs, pancreas and kidney whereas, the weight of rats did not show significant difference (p > 0.05) between the control and the treated groups. The study showed that S. hermonthica caused hepatotoxicity and could be potentially harmful for use. A more comprehensive research is recommended to investigate on its safe use and mode of action.

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