scholarly journals Injectable Hydrogels: Microskin‐Inspired Injectable MSC‐Laden Hydrogels for Scarless Wound Healing with Hair Follicles (Adv. Healthcare Mater. 10/2020)

2020 ◽  
Vol 9 (10) ◽  
pp. 2070029
Author(s):  
Xin Zheng ◽  
Zhaozhao Ding ◽  
Weinan Cheng ◽  
Qiang Lu ◽  
Xiangdong Kong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hair Follicles ◽  
Injectable Hydrogels

scholarly journals Novel scaffold based graphene oxide doped electrospun iota carrageenan/polyvinyl alcohol for wound healing and pathogen reduction: in-vitro and in-vivo study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marwa H. Gouda ◽  
Safaa M. Ali ◽  
Sarah Samir Othman ◽  
Samia A. Abd Al-Aziz ◽  
Marwa M. Abu-Serie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Hematological Parameters ◽  
Nutritional Supplement ◽  
Hair Follicles ◽  
Nanofiber Scaffold ◽  
Novel Scaffold

AbstractWound healing is a complicated multicellular process that involves several kinds of cells including macrophages, fibroblasts, endothelial cells, keratinocytes and platelets that are leading to their differentiation towards an anti-inflammatory response for producing several chemokines, cytokine and growth factors. In this study, electrospun nanofiber scaffold named (MNS) is composed of polyvinyl alcohol (PVA)/iota carrageenan (IC) and doped with partially reduced graphene oxide (prGO) that is successfully synthesized for wound healing and skin repair. The fabricated MNS was tested in case of infection and un-infection with E. coli and Staphylococcus and in both of the presence and in the absence of yeast as a natural nutritional supplement. Numerous biochemical parameters including total protein, albumin, urea and LDH, and hematological parameters were evaluated. Results revealed that the MNS was proved to be effective on most of the measured parameters and had exhibited efficient antibacterial inhibition activity. Whereas it can be used as an effective antimicrobial agent in wound healing, however, histopathological findings confirmed that the MNS caused re-epithelialization and the presence of yeast induced hair follicles growth and subsequently it may be used to hide formed head wound scar.

scholarly journals Topical hydrogel containing Fumaria vaillantii Loisel. extract enhances wound healing in rats

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Fatemeh Davoodi-Roodbordeii ◽  
Minoo Afshar ◽  
Fatemeh Haji Abas Tabrizi ◽  
Samira Choopani ◽  
Giti Torkaman ◽  
...  
Keyword(s):  
Wound Healing ◽  
Breaking Strength ◽  
Negative Control ◽  
Skin Wound ◽  
Hair Follicles ◽  
Uniaxial Tensile ◽  
Total Extract ◽  
Collagen Formation ◽  
Significant Difference ◽  
Incision Wound

Abstract Background Fumaria species (Fumariacea) has traditionally been used in wound healing in Iranian folk medicine. However, with the discovery of newer agents, its use has faded off into total obscurity. This study explored the wound healing potential of a gel containing 10% Fumaria vaillantii Loisel through topical application of total extract in a model of excisional as well as incisional wound healing in albino Wistar rats. Methods Rats were anesthetized, and excisional skin wound was established using a sterilized surgical scissors. The animals were then treated with 10% F.vaillantii topical gel formulation along with the gel base. The treatments were administered once a day after the injury for 21 days. For topical treatment, the hydrogel was formulated and evaluated for chemical and physical characteristics. Histopathological analysis with hematoxylin and eosin (H&E) was used for microscopic examination of the skin tissues on 21-day-old sections of excision wound. To verify collagen formation, hydroxyproline determination was performed 21 days post wound healing. Breaking strength was determined in a 10-day-old incision wound by the uniaxial tensile test. Results Topical administration of F.vaillantii gel formulation significantly enhanced skin wound closure on the 6th post-wounding day compared to both gel base and the negative control, indicating an accelerated wound healing process, while a significant difference was observed on 10th and 14th post –wound days in F.vaillantii treatment compared to the negative control groups. Gel formulation prepared with a 10% F. vaillantii extract exhibited a response in terms of wound epithelialization, angiogenesis and number of hair follicles at wound area better than the gel base on the 21st post-wound day. Application of gel base produced further advantages by increasing hydroxyproline content and collagen fiber thickness. Our results on incision wound model were supported by histopathological data indicating the role of gel base in the enhancement of breaking strength. Conclusion Traditional use of Fumaria species in the skin diseases was justified in this study by revealing the increase in wound healing activity after hydrogel containing F. vaillantii total extract administration. Graphical abstract

Dually crosslinked injectable hydrogels of poly(ethylene glycol) and poly[(2-dimethylamino)ethyl methacrylate]-b-poly(N-isopropyl acrylamide) as a wound healing promoter

2017 ◽  
Vol 5 (25) ◽  
pp. 4955-4965 ◽  
Author(s):  
Arvind K. Singh Chandel ◽  
Deepika Kannan ◽  
Bhingaradiya Nutan ◽  
Shailja Singh ◽  
Suresh K. Jewrajka
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
Tissue Regeneration ◽  
Poly Ethylene Glycol ◽  
Small Molecular Weight ◽  
Injectable Hydrogels ◽  
Soft Tissue Regeneration ◽  
Isopropyl Acrylamide ◽  
Poly Ethylene ◽  
By Products

PEG-based dually crosslinked injectable hydrogels have been developed through extremely simple chemistry which avoids use of small molecular weight crosslinker, formation of by-products and involved low heat change. The hydrogels are useful for wound healing and soft tissue regeneration.

scholarly journals Immunohistological changes in skin wounds during the early periods of healing in a rat model

2012 ◽  
Vol 57 (No. 2) ◽  
pp. 77-82 ◽  
Author(s):  
F. Sabol ◽  
L. Dancakova ◽  
P. Gal ◽  
T. Vasilenko ◽  
M. Novotny ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wide Spectrum ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Skin Wound ◽  
Hair Follicles ◽  
In Vivo Model ◽  
Wound Healing Process ◽  
Rat Skin

The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin 14, α-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

Preparation and Comparison of Effects of Different Herbal Oil Ointments as Wound-Healing Agents

2019 ◽  
Vol 207 (3-4) ◽  
pp. 177-186 ◽  
Author(s):  
Alireza Valizadeh ◽  
Mahdieh Shirzad ◽  
Mohammad Reza Pourmand ◽  
Maryam Farahmandfar ◽  
Hassan Sereshti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Dorsal Surface ◽  
Inflammatory Cells ◽  
Hair Follicles ◽  
Sesame Oil ◽  
Full Thickness ◽  
Olive Oils ◽  
Complex Process ◽  
And Control

Wound healing is a complex process and some agents have been reported to accelerate it. The aim of this study was to evaluate the healing effect of Eucerin-based ointments of lemon, sesame and olive oils on infected full-thickness wounds in rats. Wounds were created on the dorsal surface of Male Albino Wistar rats (n = 12). Wounds were treated with an Eucerin-based ointment containing either of lemon, sesame or olive oils (33% w/w) twice a day for 14 days. Histopathology results showed that contraction of wounds treated with lemon and sesame oils was higher than in the olive oil and control groups on days 10 and 14. In the lemon- and sesame-oil treated groups, on day 14, 50% of rat lesions were completely healed. Total number of inflammatory cells in lemon oil treatment group was significantly smaller than that of others on day 14 (p < 0.001). Also, thickness of the epidermal layer and rejuvenation of the hair follicles and other skin appendages was normal in lemon and sesame oil treated groups. The lemon and sesame oil ointments accelerated the healing process of wounds in macroscopic, morphological and morphmetrical analyses. Therefore, lemon and sesame oil ointments could be considered as alternative dressings for infected full-thickness wounds because of improved wound healing characteristics.

scholarly journals Effect and Repair Mechanism of Nano Ag Sponge Dressing Combined with Gelatin-Bletilla Striata Gum/Salvia Miltiorrhiza on Refractory Orthopedic Wounds

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Chuanjie Jiao ◽  
Ming Deng ◽  
Yonggang Ma ◽  
Geliang Hu
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Rate ◽  
Salvia Miltiorrhiza ◽  
Collagen Fibers ◽  
Hair Follicles ◽  
Elastic Fibers ◽  
Bletilla Striata ◽  
Ctrl Group ◽  
Tgf Β1

Objective. To explore the effect and mechanism of the sponge dressing on the healing of refractory orthopedic wound, and the gelatin-Bletilla striata gum/Salvia miltiorrhiza nano Ag (GBS-Ag) sponge dressing was prepared. Methods. GBS-Ag sponge dressing was prepared by the freeze-drying method. Twenty male SD rats were randomly divided into the control group (Ctrl group) and GBS-Ag group, with 10 rats in each group, and the rats in the two groups were established a model of back wound infection. The Ctrl group was treated with gauze, while the GBS-Ag group was treated with GBS-Ag sponge dressing. Wound healing rate, blood immune indexes, Ag content in each organ, morphological changes of wound, and expression of transforming growth factor-β1 (TGF-β1) in wound transformation were detected in the two groups of rats. Results. The mechanical properties of GBS-Ag sponge dressing were all in line with the standard, and it had good killing effect on the conventional strain after being incubated for 24 hours. Compared with the Ctrl group, the healing rate and lymphocyte percentage in the GBS-Ag group were significantly increased on day 4 and day 10 ( P < 0.05 ), while the total number of white blood cells and the percentage of neutrophils were significantly decreased ( P < 0.05 ). Compared with Ctrl group, the Ag content in liver, spleen, and kidney of rats in the GBS-Ag group was significantly increased ( P < 0.05 ). The histological results showed that the Ctrl group lacked collagen fibers in the dermis, and the angiogenesis was not rich, accompanied by a large number of inflammatory cell infiltration. The epidermal repair of rats in the GBS-Ag group was complete and partially keratinized, the dermis was rich in collagen fibers, with elastic fibers and new blood vessels, inflammatory cells were rare, and new hair follicles and thick-walled blood vessels were also observed. The expression of TGF-β1 protein in the wounds of rats in the GBS-Ag group was higher than that of the Ctrl group. Conclusion. GBS-Ag sponge dressing had multiple effects of sterilization and promoting wound healing, and its mechanism may be related to promoting the TGF-β1 protein expression.

scholarly journals Human Amniotic Fluid Stem Cell-Derived Exosomes as a Novel Cell-Free Therapy for Cutaneous Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Yan Zhang ◽  
Jiaqing Yan ◽  
Yanhong Liu ◽  
Zhenyu Chen ◽  
Xiheng Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Fluid ◽  
Transforming Growth Factor ◽  
Therapeutic Potential ◽  
Dermal Fibroblast ◽  
Hair Follicles ◽  
Receptor Type ◽  
Type I ◽  
Human Amniotic Fluid ◽  
Β Receptor

Adult wound healing often results in fibrotic scarring that is caused by myofibroblast aggregation. Human amniotic fluid stem cells (hAFSCs) exhibit significantly anti-fibrotic scarring properties during wound healing. However, it is little known whether hAFSCs directly or indirectly (paracrine) contribute to this process. Using the full-thickness skin-wounded rats, we investigated the therapeutic potential of hAFSC-derived exosomes (hAFSC-exo). Our results showed that hAFSC-exo accelerated the wound healing rate and improved the regeneration of hair follicles, nerves, and vessels, as well as increased proliferation of cutaneous cells and the natural distribution of collagen during wound healing. Additionally, hAFSC-exo suppressed the excessive aggregation of myofibroblasts and the extracellular matrix. We identified several miRNAs, including let-7-5p, miR-22-3p, miR-27a-3p, miR-21-5p, and miR-23a-3p, that were presented in hAFSC-exo. The functional analysis demonstrated that these hAFSC-exo-miRNAs contribute to the inhibition of the transforming growth factor-β (TGF-β) signaling pathway by targeting the TGF-β receptor type I (TGF-βR1) and TGF-β receptor type II (TGF-βR2). The reduction of TGF-βR1 and TGF-βR2 expression induced by hAFSC-exo was also confirmed in the healing tissue. Finally, using mimics of miRNAs, we found that hAFSC-exo-miRNAs were essential for myofibroblast suppression during the TGF-β1-induced human dermal fibroblast-to-myofibroblast transition in vitro. In summary, this study is the first to show that exosomal miRNAs used in hAFSC-based therapy inhibit myofibroblast differentiation. Our study suggests that hAFSC-exo may represent a strategic tool for suppressing fibrotic scarring during wound healing.

scholarly journals Microskin‐Inspired Injectable MSC‐Laden Hydrogels for Scarless Wound Healing with Hair Follicles

2020 ◽  
Vol 9 (10) ◽  
pp. 2000041 ◽  
Author(s):  
Xin Zheng ◽  
Zhaozhao Ding ◽  
Weinan Cheng ◽  
Qiang Lu ◽  
Xiangdong Kong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hair Follicles

scholarly journals A Novel Composite Hydrogel Composed of Formic Acid-Decellularized Pepsin-Soluble Extracellular Matrix Hydrogel and Sacchachitin Hydrogel as Wound Dressing to Synergistically Accelerate Diabetic Wound Healing

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 538
Author(s):  
Chien-Ming Hsieh ◽  
Weu Wang ◽  
Ying-Hsuan Chen ◽  
Pu-Sheng Wei ◽  
Yu-Hsuan Liu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Formic Acid ◽  
Cell Viability ◽  
Hair Follicles ◽  
Composite Hydrogel ◽  
Sweat Glands ◽  
Diabetic Wound ◽  
Porcine Skin ◽  
Diabetic Wound Healing

Extracellular matrix (ECM) hydrogel can create a favorable regenerative microenvironment and act as a promising dressing for accelerating the healing of diabetic wound. In this study, a simple and effective decellularization technique was developed and optimized to obtain acellular extracellular matrix (aECM) from porcine skin. It was found that decellularization at 30% formic acid for 72 h effectively decellularized porcine skin while retaining >75% collagen and ~37% GAG in the aECM with no presence of nuclei of cellular remnants. aECM hydrogel was fabricated by digesting aECM with pepsin in various acidic solutions (0.1 N HCl, glycolic acid (GA) and 2-pyrrolidone-5-carboxylic acid (PCA)) and then treated with a pH-controlled neutralization and temperature-controlled gelation procedure. Based on physical characterizations, including SDS-PAGE, rheological analysis and SEM analysis, aECMHCl hydrogels fabricated at 25 mg/mL in 0.1 N HCl were selected. Four polymeric ECM-mimic hydrogels, including sacchachitin (SC), hyaluronic acid (HA) and chitosan (CS) and three composite hydrogels of combining SC either with aECMHCl,25 (aECMHCl/SC), HA (HA/SC) or CS (SC/CS) were prepared and evaluated for WS-1 cell viability and wound-healing effectiveness. Cell viability study confirmed that no hydrogel dressings possessed any toxicity at all concentrations examined and ECMHCl, HA and ECMHCl/SC at higher concentrations (>0.05%) induced statistically significant proliferation. Diabetic wound healing study and histological examinations revealed that ECMHCl/SC hydrogel was observed to synergistically accelerate wound healing and ultimately stimulated the growth of hair follicles and sweat glands in the healing wound indicating the wound had healed as functional tissues. The results support the great potential of this newly produced ECMHCl/SC composite hydrogel for healing and regeneration of diabetic wounds.

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