Bioassay‐Guided Fractionation, Chemical Compositions and Antibacterial Activity of Extracts from Rhizomes of Globba schomburgkii H ook.f.

2020 ◽  
Vol 17 (8) ◽  
Author(s):  
Naruemon Suekaew ◽  
Sarisa Na Pombejra ◽  
Chadin Kulsing ◽  
Jeerapat Doungchawee ◽  
Tanatorn Khotavivattana
Keyword(s):  
Antibacterial Activity ◽  
Chemical Compositions ◽  
Bioassay Guided Fractionation

scholarly journals Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1

2017 ◽  
Vol 66 (4) ◽  
pp. 449-461 ◽  
Author(s):  
Sahar W.M. Hassan ◽  
Hassan A.H. Ibrahim
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Basal Medium ◽  
Chemical Characterization ◽  
Microscopic Analysis ◽  
Fold Increase ◽  
Chemical Compositions ◽  
Malt Extract ◽  
Electron Microscopic ◽  
Potential Applications

Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic. Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields. The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications. Effect of medium type, incubation period and pH were studied using the one factor at a time experiments. It was shown that the highest productivity (24 gl–1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation. Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations. The finalized optimized medium contained (gl–1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl–1 with1.4 fold increase compared to the basal medium. Chemical characterization of the extracted EPS showed that, FTIR spectra exhibited bands at various regions. Moreover, HPLC chromatogram indicated that the EPS was a heteropolysaccharide consisting of maltose and rhamnose. The study was extended to evaluate the potentiality of the extracted polysaccharides in different medical applications. Results concluded that, EPS exhibited antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis and the highest antibacterial activity (7.8, 9 and 10.4 AU/ml) was against S. faecalis at 50, 100 and 200 mg/ml respectively. The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2). In addition, EPS exhibited antiviral activity at 500 μg/ml. The antioxidant capacity increased with increasing the concentration of the sample. Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.

scholarly journals Chemical compositions, antioxidant and antimicrobial activities of Tubu (Pycnarrhena longifolia) leaves used as ingredient in traditional functional foods

Food Research ◽  
2020 ◽  
Vol 4 (3) ◽  
pp. 823-830
Author(s):  
N.K. Mohammed ◽  
B.J. Muhialdin ◽  
N.S. Masri ◽  
R. Sukor ◽  
F. Abd-El Aziem ◽  
...  
Keyword(s):  
Amino Acids ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Sugar Content ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Chemical Compositions ◽  
Food Ingredient

Tubu (Pycnarrhena longifolia) leaves are known as food enhancer and medicinal plant by several ethnics of Malaysia. The aims of the study were to determine the tubu leaves composition and biological activity including antioxidant and antibacterial. The mineral, sugar and amino acids were determined using HPLC, and bioactive compounds using GCMS. The antioxidant activity was evaluated by DPPH assay, and the antibacterial activity screened by disc diffusion method. The dry leaf compositions were 6.6% moisture, 11.6% ash, 7.0% crude protein, 15.7% crude fiber, 12.1% crude fat and carbohydrate 47%. A total of seven minerals were present in the methanol extract and the highest content was for iron (159.5 mg) and calcium (16.8 mg). The sugar profile showed high sugar content for glucose, sucrose, rhamnose, and fructose which was 8, 13, 7, and 20 mg/g, respectively. A total of sixteen amino acids was present in the tubu leaf extract, and seven bioactive compounds were identified by GC-MS/MS. The antioxidant activity was 87%, total phenolic compound content was 26.49 mg/mL and total flavonoid content was 13.39 mg/mL. Tubu leaves showed antibacterial activity towards Salmonella enterica serovar Typhimurium, Escherichia coli, Bacillus cereus and Staphylococcus aureus. This study showed that tubu leaves had rich sources of amino acids, minerals, sugars and bioactive compounds, potential to be a functional food ingredient.

scholarly journals Bioassay Guided Fractionation of Senna singueana and Its Potential for Development of Poultry Phytogenic Feed Additives

2022 ◽  
Vol 8 ◽  
Author(s):  
Prosper Jambwa ◽  
Fikile N. Makhubu ◽  
Gift Matope ◽  
Gerda Fouche ◽  
Lyndy J. McGaw
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Xylenol Orange ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Feed Additives ◽  
Lipoxygenase Activity ◽  
Isolation And Identification ◽  
Bioassay Guided Fractionation

There has been burgeoning interest in plant-based feed additives following restrictions placed on the use of antibiotic feed additives in many countries. Phytogenic feed additives are recommended to have a range of useful properties to support the growth and development of poultry to a similar level as that obtained by supplementing feed with antibiotics. The aim of this study was to evaluate the antibacterial, anti-lipoxygenase and antioxidant activity, and in vitro safety of fractions and isolated compounds from leaves of Senna singueana. Antibacterial activities of the fractions and isolated compounds were determined against a panel of bacteria using a two-fold serial microdilution assay and qualitative bioautography assays. Anti-lipoxygenase activity was evaluated using the ferrous oxidation-xylenol orange (FOX) method. Antioxidant activity was assessed qualitatively and quantitatively using radical scavenging assays. Dichloromethane and ethyl acetate fractions from solvent-solvent partitioning had the best antibacterial activity with MIC values ranging from 156 to 313 μg/ml. Fractions obtained from column chromatography had significant to weak antibacterial activity with MIC values ranging from 50 to 1,250 μg/ml. Bioautography showed clear bands of bacterial inhibition, indicating the presence of a number of active compounds in several fractions. The ethyl acetate fraction and all the tested column fractions had potent anti-lipoxygenase activity with IC50 values of ≤2.5 μg/ml which were lower than that of quercetin (positive control), indicating anti-inflammatory potential. The ethyl acetate fraction and several column fractions had powerful antioxidant activity with IC50 values of ≤5 μg/ml in the ABTS assay. Cytotoxicity values against Vero kidney cells ranged from LC50 = 40.0–989.3 μg/ml. Bioassay-guided fractionation led to the isolation and identification of a known bioactive compound, luteolin. S. singueana is a promising candidate for the development of poultry phytogenic feed additives.

scholarly journals Proximate, Amino Acid Composition and Antibacterial Activity of Woton (Sterculia sp.) Leaves from Raja Ampat, West Papua, Indonesia

Jurnal Airaha ◽  
2019 ◽  
Vol 8 (01) ◽  
pp. 001-007
Author(s):  
Mohammad Sayuti ◽  
Iman Supriatna ◽  
Intanurfemi B. Hismayasari ◽  
Ahmad Yani ◽  
Saidin
Keyword(s):  
Antibacterial Activity ◽  
Amino Acid ◽  
Chemical Composition ◽  
Standard Procedure ◽  
Chemical Compositions ◽  
Endemic Plant ◽  
Inhibition Response ◽  
West Papua ◽  
Chemical Content ◽  
Amino Acid Contents

Woton (Sterculia sp.) plant is an endemic plant of  Papua and West Papua, Indonesia that has many benefits for the community. This research aims to examine the contents of proximate, amino acid, and antibacterial activity from the leaves extract of two plants namely Sterculia shilinglawii and Sterculia tragacantha. The sample of Sterculia sp. leaves are cleaned, dried, and milled. Then, its chemical composition, amino acid, and bacterial activity are analyzed using a standard procedure. The chemical composition was analyzed using standard AOAC, amino acid was examined using LCMS and antibacterial activity was analyzed using barrier zone against the bacteria that attack the fish. The research results showed that the chemical compositions of Sterculia sp. leaves are carbohydrates, protein, water, ash and fat. The main amino acid contents of woton (Sterculia sp.) leaves are L-Arginine, L-Valine, L-Proline, L-Alanine. Bacterial Test Results showed  that woton (Sterculia sp.) leaves extract has intermediate inhibition response against V. algynoliticus. It has been concluded that Sterculia sp. leaves have the potential chemical content.

scholarly journals Evaluation of the Antidiabetic and Antibacterial Activity of Cissus sicyoides

2002 ◽  
Vol 45 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Flavio Luis Beltrame ◽  
Greisiele Lorena Pessini ◽  
Dani Luce Doro ◽  
Benedito Prado Dias Filho ◽  
Roberto Barbosa Bazotte ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Minimal Inhibitory Concentrations ◽  
Aerial Parts ◽  
Bioassay Guided Fractionation

In this work we investigated the antidiabetic and antibacterial effect of Cissus sicyoides (CS) from Brazil. Diabetic rats that received water (A group) or extracts from the aerial parts of the plant (Cs group) during four weeks were employed. After this period, serum levels of glucose, cholesterol and triglycerides were measured. Glycemia was not affected by treatment with CS. However, there was an increased cholesterol and triglyceride level in Cs group. In addition, bioassay-guided fractionation of methanolic extract from aerial parts of CS was performed for isolation of antibacterial compounds.beta-Sitosterol and sitosterol-beta-D-glucopyranoside isolated showed antibacterial activity against Bacillus subtilis with minimal inhibitory concentrations (MICs) of 50 mug/ml and 100 mug/ml, respectively. In spite of popular belief, CS did not show antidiabetic activity. However, two compounds isolated from aerial parts of the plant (beta-sitosterol and sitosterol-beta-D-glucopyranoside) showed antibacterial activity.

scholarly journals Identification of a New Antibacterial Sulfur Compound fromRaphanus sativusSeeds

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Jeries Jadoun ◽  
Ahmad Yazbak ◽  
Salwa Rushrush ◽  
Amira Rudy ◽  
Hassan Azaizeh
Keyword(s):  
Antibacterial Activity ◽  
Sulfamic Acid ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Plant Parts ◽  
H Nmr ◽  
Microbial Infections ◽  
Bioassay Guided Fractionation ◽  
Isolated Compound ◽  
Red Radish

Raphanus sativusL. (radish), a member of Brassicaceae, is widely used in traditional medicine in various cultures for treatment of several diseases and disorders associated with microbial infections. The antibacterial activity of the different plant parts has been mainly attributed to several isothiocyanate (ITC) compounds. However, the low correlation between the ITC content and antibacterial activity suggests the involvement of other unknown compounds. The objective of this study was to investigate the antibacterial potential of red radish seeds and identify the active compounds. A crude ethanol seed extract was prepared and its antibacterial activity was tested against five medically important bacteria. The ethanol extract significantly inhibited the growth of all tested strains. However, the inhibitory effect was more pronounced againstStreptococcus pyogenesandEscherichia coli. Bioassay-guided fractionation of the ethanol extract followed by HPLC,1H-NMR,13C-NMR,15N-NMR, and HMBC analysis revealed that the active fraction consisted of a single new compound identified as [5-methylsulfinyl-1-(4-methylsulfinyl-but-3-enyl)-pent-4-enylidene]-sulfamic acid, which consisted of two identical sulfur side chains similar to those found in ITCs. The minimal inhibitory concentration values of the isolated compound were in the range of 0.5–1 mg/mL. These results further highlight the role of radish as a rich source of antibacterial compounds.

scholarly journals Antioxidant and Antibacterial Activity of Roseroot (Rhodiola rosea L.) Dry Extracts

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1767 ◽  
Author(s):  
Olga Kosakowska ◽  
Katarzyna Bączek ◽  
Jarosław L. Przybył ◽  
Ewelina Pióro-Jabrucka ◽  
Weronika Czupa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Rhodiola Rosea ◽  
Array Detector ◽  
Chemical Compositions ◽  
Antioxidant Power ◽  
Scavenging Capacity ◽  
Chemical Profiles

Roseroot (Rhodiola rosea L.) belongs to plants revealing adaptogenic properties, which are attributed to the presence of specific phenolic compounds and are reflected mainly as antioxidant activity. The aim of the present study was to determine the antioxidant and antibacterial activity of various products obtained from R. rosea (underground organs as well as their aqueous and ethanolic dry extracts) in relation to the chemical profiles of phenolic and essential oil compounds. The chemical profiles were determined by High-performance Liquid Chromatography with a diode-array detector (HPLC-DAD) and Gas chromatography-mass spectrometry (GC-MS), antioxidant activity by (1,1-Diphenyl-2-picrylhydrazyl) Scavenging Capacity Assay (DPPH), (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) Scavenging Capacity Assay (ABTS) and Ferric Reducing Antioxidant Power Assay (FRAP) and antimicrobial properties were expressed as minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) values following the broth microdilutions method. The results show that the investigated samples differed in terms of their chemical compositions and biological activities. The extracts were more abundant in phenolic compounds (salidroside, tyrosol, and rosavin derivatives) in comparison to dried underground organs. The content of the determined phenolics in the analyzed extracts was affected by the solvent used for extraction. The ethanolic extract was characterized by the highest content of these substances in comparison to the aqueous one and the dried raw material, especially with regard to rosavin (969.71 mg/100 g). In parallel, this extract showed the strongest antioxidant and antibacterial activity. However, dried R. rosea underground organs also revealed strong antibacterial effects against, for example, Staphylococcus strains.

scholarly journals Chemical composition, antimicrobial activity and chromosome number of Hertia cheirifolia L. from Algeria

2020 ◽  
Vol 7 (2) ◽  
pp. 31-43
Author(s):  
Ounoughi Abdelkader ◽  
Ramdani Messaoud ◽  
Lograda Takia ◽  
Chalard Pierre ◽  
Figueredo Gilles
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Chromosome Number ◽  
Essential Oil ◽  
Essential Oils ◽  
Diffusion Method ◽  
Chemical Compositions ◽  
Root Cells ◽  
Karyotypic Analysis ◽  
Germacrene D

AbstractThe aims of this work are to investigate the chemical composition, the antibacterial activity of the essential oil and the chromosome numbers of two populations of Hertia cheirifolia. The samples were collected in the flowering stage, in eastern Algeria locality. The aerial parts of H. cheirifolia were submitted to a hydro-distillation. GC and GC / MS analysed the chemical compositions of the obtained essential oils. The antibacterial activity of essential oils was evaluated using the disks diffusion method against ten bacterial strains. For karyotypic analysis, the squashing method is used. Fifty-eight compounds representing 98.93% of the total oil were identified in H. cheirifolia. The chemical composition is dominated by the presence of major products, α-pinene (48.49 - 53.85%) and Germacrene-D (2.64 - 12.66%). Two distinct chemical breeds were identified, the α-pinene-spathulenol of Batna population, and the α-pinene-germacrene-D of Setif population. The essential oil of H. cheirifolia has a moderate activity against bacteria tested. In contrast, the strains E. coli ATCC 25922, P. syringae ATCC 53543 and E. fecalis ATCC 49452 are resistant to H. cheirifolia essential oils. The observations of root cells meristematic at metaphase of H. cheirifolia gave a diploid chromosome number 2n = 2x = 20, with a basic chromosome number (x= 10).

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