scholarly journals Evaluation of the Antidiabetic and Antibacterial Activity of Cissus sicyoides

2002 ◽  
Vol 45 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Flavio Luis Beltrame ◽  
Greisiele Lorena Pessini ◽  
Dani Luce Doro ◽  
Benedito Prado Dias Filho ◽  
Roberto Barbosa Bazotte ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Minimal Inhibitory Concentrations ◽  
Aerial Parts ◽  
Bioassay Guided Fractionation

In this work we investigated the antidiabetic and antibacterial effect of Cissus sicyoides (CS) from Brazil. Diabetic rats that received water (A group) or extracts from the aerial parts of the plant (Cs group) during four weeks were employed. After this period, serum levels of glucose, cholesterol and triglycerides were measured. Glycemia was not affected by treatment with CS. However, there was an increased cholesterol and triglyceride level in Cs group. In addition, bioassay-guided fractionation of methanolic extract from aerial parts of CS was performed for isolation of antibacterial compounds.beta-Sitosterol and sitosterol-beta-D-glucopyranoside isolated showed antibacterial activity against Bacillus subtilis with minimal inhibitory concentrations (MICs) of 50 mug/ml and 100 mug/ml, respectively. In spite of popular belief, CS did not show antidiabetic activity. However, two compounds isolated from aerial parts of the plant (beta-sitosterol and sitosterol-beta-D-glucopyranoside) showed antibacterial activity.

scholarly journals Antidiabetic and anti-obesity acylated flavonol diglucoside from Ammannia baccifera L. subsp. aegyptiaca (Willd.) Koehne Waste

2020 ◽  
Author(s):  
Noha Swilam ◽  
Mahmoud Nawwar ◽  
Rasha Radwan ◽  
Eman Mostafa
Keyword(s):  
Binding Free Energy ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
High Serum ◽  
Antidiabetic Activity ◽  
Active Site Residues ◽  
Sod Activity ◽  
Aerial Parts

Abstract Chemical investigation of the aerial parts of Ammania aegyptiaca ethanol extract (AEEE) revealed significant high concentrations of polyphenols and flavonoids content with notable antioxidant activity in DPPH, ORAC, and reducing power assay. New acylated diglucoside flavonol myricetin 3-O-β-4­C1-(6"-O-galloyl glucopyranoside) 7-O-β-4C1-glucopyranoside (MGGG) was isolated from aerial parts of AEEE along with four additional known phenolics, not characterized previously from AEEE. Moreover, powerful inhibitory effects of MGGG, AEEE, and all isolates against α-amylase, pancreatic lipase and β-glucosidase, were assessed. In addition, flexible molecular docking was used to reveal the inhibition towards digestive enzymes and confirmed that the MGGG interacted strongly with the active site residues of these enzymes with the highest binding free energy against β-glucosidase (DG=-8.98 kcal/mol) compared to the commercial drug Acarbose, thus justifying its dual management of diabetes and obesity. In streptozotocin (STZ) induced diabetic rats, AEEE significantly decreased high serum glucose, α-amylase activity, liver and kidney function markers and increased insulin level. Moreover, it improved lipid profile due to diabetes with increased SOD activity and inhibited of TBARS formation. Consequently, AEEE and MGGG are found useful in controlling the secondary complications associated with type 2 diabetes mellitus. Histopathological studies proved the decrease in the pancreas damage and agreed with the biochemical findings. These results provide evidence that AEEE and MGGG have potent antidiabetic activity, which warrants additional investigations.

scholarly journals Antibacterial Alkaloids from Chelidonium Majus Linn (Papaveraceae) Against Clinical Isolates of Methicillin-Resistant Staphylococcus Aureus

2009 ◽  
Vol 11 (4) ◽  
pp. 90 ◽  
Author(s):  
Guo Ying Zuo ◽  
Fan Yan Meng ◽  
Xiao Yan Hao ◽  
Yun Ling Zhang ◽  
Gen Chun Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Dilution Method ◽  
Chelidonium Majus ◽  
Methicillin Resistant ◽  
Aerial Parts ◽  
Bioassay Guided Fractionation ◽  
Mrsa Strains

Purpose. This study describes the antibacterial effect of extracts and compounds isolated from the aerial part of Chelidonium majus Linn. (Papaveraceae) acting against clinical strains of methicillin-resistant Staphylococcus aureus (MRSA). Methods. The activities were evaluated by using the macrobroth dilution method and reported as the MICs/MBCs. Results. Bioassay-guided fractionation of the most active extract from the aerial parts (EtOAc) led to the isolation of benzo[c]phenanthridine-type alkaloids 8-hydroxydihydrosanguinarine (hhS), 8-hydroxydihydrochelerythrine (hhC), which were potently active against MRSA strains. Conclusions. The selective antibacterial activity reported in this paper for 8-hydroxylated benzo[c]phenanthridine-type alkaloids isolated from C.majus opens the possibility that they could be helpful for the developing of new antibacterial agents for treating the infection of MRSA which has created nosocomial problem worldwide.

scholarly journals ANTIDIABETIC ACTIVITY OF KALANCHOE PINNATA IN ALLOXAN-INDUCED DIABETIC RATS

2019 ◽  
pp. 241-245
Author(s):  
LATHA OPHELIA GEORGE ◽  
RADHA HR ◽  
SOMASHEKARAIAH BV
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Methanolic Extract ◽  
Public Health Problem ◽  
Diabetic Rats ◽  
The Body ◽  
Antidiabetic Activity ◽  
Percentage Increase ◽  
Group I ◽  
Kalanchoe Pinnata

Objective: The main purpose of the present study was to analyze the antidiabetic activity of methanolic extract of the leaf of Kalanchoe pinnata in alloxan-induced diabetic rats. Methods: Diabetes mellitus (DM) is a chronic metabolic and endocrine disease regarded as a serious global public health problem. Albino Wistar rats were divided into four groups. Group I (normal) received drinking water throughout the course till 20 days. Groups II-IV received alloxan (120 mg/kg b.w) i.p on the 1st day of the study period. Group III animals received glibenclamide (10 mg/kg p.o) and Group IV received K. pinnata (200 mg/kg b.w p.o) for 20 days. The body weight, blood glucose level, serum urea, blood urea nitrogen (BUN), creatinine, cholesterol, total protein (TP), uric acid, and tissue (liver) antioxidant parameters: Malondialdehyde (MDA) and glutathione (GSH) were measured. Results: K. pinnata treated rats showed the percentage increase in the body weight, decrease in the blood sugar level, creatinine, TP level, urea, uric acid, and BUN, MDA, and GSH when compared to alloxan-induced diabetic mellitus control rats. Thus, K. pinnata could be possibly employed to treat DM. Conclusion: This preliminary analysis tested the most promising profile. It seems that methanolic extract of the leaf of K. pinnata improved general, blood serum, and liver antidiabetic agent. However, further studies confirming its potential is certainly warranted.

scholarly journals Antibacterial Activity of Cuttlefish Sepia sp. (Cephalopoda,) Ink Extract Against Aeromonas hydrophila

2019 ◽  
Vol 24 (3) ◽  
pp. 184
Author(s):  
R Adharyan Islamy
Keyword(s):  
Antibacterial Activity ◽  
Aeromonas Hydrophila ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Positive Control ◽  
Average Yield ◽  
Antibacterial Test ◽  
Treatment Dose ◽  
Dried Extract ◽  
Antiretroviral Activity

Cephalopods ink has shown potential antiretroviral activity. The ink extracts of cuttlefish showed an antibacterial effect. This study aims to investigate the antibacterial activity of the methanolic extract of the ink of cuttlefish Sepia sp. against Aeromonas hydrophila. A. hydrophila are opportunists and associated with aquatic fish and shrimp disease. The shade-dried ink sample from approximately 30g ink sacs obtained from 15 animals was immersed separately in methanol (1:3 w/v) solvents for overnight. The experiment in this study used the dried extract of cuttlefish ink. The isolate of A. hydrophila was originated from Jepara Brackishwater Aquaculture Center. The average yield percentage of cuttlefish ink extract obtained was 4.86%. The results of the MIC test in Table V show that the highest average absorbance value obtained was at a concentration of 50 ppm, which was equal to 1.716 nm, and the lowest absorbance was obtained at a treatment dose of 300 ppm at 0.841 nm, while the Mc Farland tube was 0.933 nm. The results of antibacterial test on Table II showed antibacterial activity of cuttlefish ink extract at negative concentration control showed diameter zone of 5 ± 1.2 mm, at positive control showed diameter zone of 31 ± 1.2 mm, at 250 ppm result 19 ± 0.9 mm, at 300 ppm result 22 ± 1.4 mm, at 350 ppm result 31 ± 1.2 mm.

scholarly journals Antibacterial activity of cis- and trans-resveratrol isolated from Polygonum cuspidatum rhizome

2006 ◽  
pp. 131-136 ◽  
Author(s):  
Zoran Kukric ◽  
Ljiljana Topalic-Trivunovic
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antibacterial Effect ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Polygonum Cuspidatum ◽  
Disk Diffusion Method ◽  
Cis And Trans

The work is concerned with the antibacterial effect of ethanolic solutions of cis- and trans-resveralrol (cis-.lrans-3,5.4'-trihydroxystilbene) obtained by the extraction (ethanol-water 1:1 v/v) of Polygonum cuspidatum rhizome. Antibacterial activity was tested by disk diffusion method on the following bacteria: Escherichia coli, Sarcina liitea. Bacillus subtilis and Staphylococcits sp., using extract concentrations of 5 mg/disk. All tests showed significant antimicrobial activity, whereby the extract with trans-resveratrol exhibited more significant effect than the extract of cis-resveratrol.

scholarly journals Antidiabetic Activity of Stems of Hemipararsite Dendrophthoe falcata Linn. Parasitic Growing on Mangifera indica

1970 ◽  
Vol 7 (2) ◽  
pp. 177-180 ◽  
Author(s):  
SJ Anarthe ◽  
RD Bhalke ◽  
RB Jadhav ◽  
SJ Surana
Keyword(s):  
Elevated Level ◽  
Total Phenolics ◽  
Methanolic Extract ◽  
Mangifera Indica ◽  
Diabetic Rats ◽  
Blood Cholesterol ◽  
Antidiabetic Activity ◽  
Blood Protein ◽  
Cytoprotective Activity ◽  
Dendrophthoe Falcata

In the present investigation, we tested the possible cytoprotective and oral hypoglycemic effects of methanolic extract of stems of Dendrophthoe falcata. Besides, total phenolics and total flavonoids were also investigated. The methanolic extract of D. falcata at 200 mg/kg has also shown significant cytoprotective activity (p < 0.05) in alloxan-induced diabetic rats. In addition the extract significantly reduced the elevated level of blood cholesterol (p < 0.01) and triglyceride (p < 0.05). At the same dose level, the extract significantly improved the alloxan-induced reduction of blood protein level (p < 0.01) to normal value. Key words: Antidiabetic activity, Hemiparasite, Dendrophthoe falcata doi: 10.3329/dujps.v7i2.2175 Dhaka Univ. J. Pharm. Sci. 7(2): 177-180, 2008 (December)

Bioassay-Guided Chromatographic Isolation and Identification of Antibacterial Compounds from Artemisia annua L. That Inhibit Clostridium perfringens Growth

2014 ◽  
Vol 97 (5) ◽  
pp. 1282-1290 ◽  
Author(s):  
Elise Ivarsen ◽  
Xavier C Fretté ◽  
Kathrine B Christensen ◽  
Lars P Christensen ◽  
Ricarda M Engberg ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Antibacterial Effect ◽  
Artemisia Annua ◽  
Synergistic Effects ◽  
Necrotic Enteritis ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Bioassay Guided Fractionation ◽  
Hexane Extracts ◽  
Slight Inhibition

Abstract Clostridium perfringens is the causative agent of necrotic enteritis leading to significant losses in the poultry industry. Dichloromethane and n-hexane extracts of aerial parts of Artemisia annua (Asteraceae) exhibited activity against C. perfringens with minimum inhibitory concentrations (MIC) of 185 and 270 μg/mL, respectively. Bioassay-guided fractionation of the extracts gave several active fractions (MIC between 75 and 600 μg/mL). Investigations of the most active fractions resulted in the iso ponticaepoxide (MIC between 100 and 200 μg/mL) and (+)-threo-(5E)-trideca-1,5-dien-7,9,11-triyne-3,4-diol (MIC between 400 and 800 μg/mL), the flavonols chrysosplenol D (MIC between 200 and 400 μg/mL) and casticin (slight inhibition at 800 μg/mL), and 2,4-dihydroxy-6-methoxyacetophenone (slight inhibition at 800 μg/mL). Also, the coumarin scopoletin and the sesquiterpene lactone artemisinin were isolated from active fractions but showed no inhibition of C. perfringens growth at 800 and 2000 μg/mL, respectively. Fractions containing essential oil components with camphor constituting &gt;60% did not show inhibition of C. perfringens up to 1600 μg/mL. Extracts and some active fractions showed higher antibacterial effect than individual bioactive compounds, suggesting that synergistic effects may underlie the observed antibacterial effect. The present study adds new valuable information on the antibacterial effect of A. annua against C. perfringens.

scholarly journals Antidiabetic Activity of Aerial Parts and Seeds of Purslane (Portulaca oleracea) on Diabetic Rats

2020 ◽  
pp. 13-23
Author(s):  
E. G. El- Dreny
Keyword(s):  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Basal Diet ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Biological Experiment ◽  
Aerial Parts ◽  
Liver And Kidney

This study has been proposed to study the effect of aerial parts and seeds of purslane on diabetic rats. Chemical composition, phenolic compounds and fatty acids of purslane and seeds powder were determined. A biological experiment was performed using 30 male albino rats. The rats were divided into six groups (five in group) and the duration of experiment was 8 weeks. Normal control (G1) non- diabetic rats fed on the basal diet, diabetic control (G2) diabetic rats fed on the basal diet only, the other groups of diabetic rats fed on basal diet supplemented with purslane (5 and 10%) and  seeds (5 and 10%). The results of biological experiment indicated that adding of purslane and seeds to basal diet for diabetic rats, led to improvement in liver and kidney functions and an increase in body weight and HDL cholesterol and decrease blood glucose, TC, TG, LDL cholesterol, v-LDL cholesterol comparing to those of diabetic rats fed on basal diet only diabetic control. Purslane (5 and 10%) and seeds (5 and 10%) were added to burger as a replacement of fat. The results of sensory evaluation indicated that all sensory properties of the burger prepared with purslane and seeds were acceptable. Through the results of this study, we recommend the possibility of using purslane and seeds to prevent and reduce the symptoms caused by diabetes.

ANTIDIABETIC ACTIVITY OF METHANOLIC EXTRACT OF CUCUMIS DIPSACEUS EHRENB. (FRUIT) IN STREPTOZOTOCIN- INDUCED DIABETIC RATS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (12) ◽  
pp. 53-61
Author(s):  
Gagandeep Kaur ◽  
◽  
D. Srivastav ◽  
Suman Lata
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Methanolic Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
The Body ◽  
Antidiabetic Activity

The present study was designed to investigate the hypoglycemic and antidiabetic properties of Cucumis dipsaceus fruit which is widely used in the Indian traditional medicine for the treatment of diabetes.Aim of present study, to investigate the antidiabetic action of the methanolic extract of C. dipsaceus fruits in Streptozotocin induced-diabetic rats. The antidiabetic activity of methanolic extract of fruit C. dipsaceus was evaluated by using normal and streptozotocin induced-diabetic rats. The acute effect of methanolic extract was evaluated by administering 500 mg/kg p.o. to normoglycemic rats. In the chronic model, the methanolic extract was administered to normal and STZ-induced-diabetic rats at dose of 250 mg/kg and 500 mg/kg p.o. per day for 21 days. Blood glucose levels and body weights were observed at specific intervals and different biochemical parameters were also monitored. Methanolic extract (500mg/kg) produced a significant reduction in blood glucose level in STZ- induced diabetic rats. The statistical data indicated a significant increase in the body weight and serum insulin level and decrease in the glycosylated hemoglobin levels. Total cholesterol level decreased while an increase in HDL and LDL cholesterol level was observed when treated with the extract. Thus the methanolic fruit extract of C. dipsaceus exhibited significant anti-hyperglycemic activities and beneficial effects in reducing the elevated total cholesterol level of STZ-induced-diabetic rats.

Sign in / Sign up

Export Citation Format

Share Document