Evaluating herbicide tolerance of industrial hemp ( Cannabis sativa L.)

Crop Science ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 419-427
Author(s):  
Michael L. Flessner ◽  
Jabari Bryd ◽  
Kevin W. Bamber ◽  
John H. Fike
Keyword(s):  
Cannabis Sativa ◽  
Herbicide Tolerance ◽  
Industrial Hemp

From residue to resource: The multifaceted environmental and bioeconomy potential of industrial hemp (Cannabis sativa L.)

2021 ◽  
Vol 175 ◽  
pp. 105864
Author(s):  
Carlo Moscariello ◽  
Silvio Matassa ◽  
Giovanni Esposito ◽  
Stefano Papirio
Keyword(s):  
Cannabis Sativa ◽  
Industrial Hemp

scholarly journals The Influence of Different Seeding Space and Organic Fertilization Level Upon Production and Mass of 1000 Seeds, Recorded in Some Hemp Varieties

2014 ◽  
Vol 71 (1) ◽  
pp. 44-48
Author(s):  
Adriana Iuliana DAN ◽  
Marcel M. DUDA ◽  
Cristina MOLDOVAN ◽  
Teodora FLORIAN
Keyword(s):  
19Th Century ◽  
Cannabis Sativa ◽  
European Countries ◽  
Physical Parameters ◽  
Organic Fertilization ◽  
Low Level ◽  
Production Values ◽  
Fertilization Level ◽  
Industrial Hemp ◽  
The 19Th Century

AbstractHemp (Cannabis sativa L.) was used for textile and cordage more than 4000 years. The cultivation of industrial hemp declined in the 19th century but it remains one of the oldest crops in history. Despite of the decline, nowadays interest for this crop has recently been renewed within various European countries (Roman et al., 2012). The aim of the research is to observe the evolution of production values and physical parameters (MMB- grain) under the influence of different seeding space and organic fertilization level recorded in some hemp varieties approved in Romania, with a low level of THC.

Effect of hot air and infrared drying on the retention of cannabidiol and terpenes in industrial hemp (Cannabis sativa L.)

2021 ◽  
Vol 172 ◽  
pp. 114051
Author(s):  
Chang Chen ◽  
Ivan Wongso ◽  
Daniel Putnam ◽  
Ragab Khir ◽  
Zhongli Pan
Keyword(s):  
Cannabis Sativa ◽  
Infrared Drying ◽  
Hot Air ◽  
Industrial Hemp

scholarly journals (‒)-Cannabidiolic Acid, a Still Overlooked Bioactive Compound: An Introductory Review and Preliminary Research

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2638 ◽  
Author(s):  
Marialuisa Formato ◽  
Giuseppina Crescente ◽  
Monica Scognamiglio ◽  
Antonio Fiorentino ◽  
Maria Tommasina Pecoraro ◽  
...  
Keyword(s):  
Seed Oil ◽  
Cannabis Sativa ◽  
Bioactive Compound ◽  
Potential Health ◽  
Cannabidiolic Acid ◽  
Health Related ◽  
Preliminary Research ◽  
Industrial Hemp ◽  
Inexpensive Procedure ◽  
Introductory Review

Cannabidiolic acid (CBDA) is the main phytocannabinoid in fiber and seed-oil hemp (Cannabis sativa L.) plants, but its potential health-related capabilities have been masked for years by a greater scientific interest towards its neutral derivative cannabidiol (CBD). This review aims to collect from the literature and critically discuss all the information about this molecule, starting from its biosynthesis, and focusing on its bioactivity, as an anti-inflammatory, anti-emetic, anti-convulsant, and anti-cancerogenic drug. Furthermore, in the awareness that, despite its multiple bioactive effects, currently poor efforts have been made to achieve its reliable purification, herein, we propose a relatively simple, fast, and inexpensive procedure for its recovery from pollen of industrial hemp cultivars. Spectroscopic and spectrometric techniques allowed us to unequivocally identify pure isolated CBDA and to distinguish it from the constitutional isomer tetrahydrocannabinolic acid (THCA-A).

scholarly journals Effect οf Genotype and Growing Year on the Nutritional, Phytochemical, and Antioxidant Properties of Industrial Hemp (Cannabis sativa L.) Seeds

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 491 ◽  
Author(s):  
Maria Irakli ◽  
Eleni Tsaliki ◽  
Apostolos Kalivas ◽  
Fotios Kleisiaris ◽  
Eirini Sarrou ◽  
...  
Keyword(s):  
High Performance ◽  
Cannabis Sativa ◽  
Antioxidant Properties ◽  
Major Effect ◽  
Total Carotenoids ◽  
Antioxidant Power ◽  
Phenolic Profiles ◽  
Ferric Reducing Antioxidant Power ◽  
Industrial Hemp ◽  
Phytochemical Components

Cannabis sativa L. seeds have been an important source of protein, oil, and dietary fiber for human and animals. Currently, there is a growing interest in the commercial products of these seeds, which are recognized as a legitimate source of medicaments, cosmeceuticals, and nutraceuticals. The objective of this study was to investigate the nutritional, phytochemical composition, and antioxidant properties of seeds from seven hemp cultivars grown in Greece for three consecutive years. All the measured parameters strongly varied under the influence of growing year and genotype. In particular, protein, oil, and carbohydrates’ content of hemp seeds as well as fatty acids’ composition were mainly affected by genotype, whereas the growing year had a major effect on phytochemical components and antioxidant activity, which was determined by the 2,2′-azino-bis (3-ethylbenzthiazoline sulfonate) (ABTS) and ferric-reducing antioxidant power (FRAP) assays. Moreover, a predominant effect of the year was observed for phenolic profiles as determined by high-performance liquid chromatography and total carotenoids’ content. This study suggests that hemp seeds could be a promising food crop as a result of their high nutritive traits and antioxidant potential. A comparison of the studied cultivars, showed that Finola seeds had the highest oil and protein contents and, thus, appeared to be the most promising cultivar for cultivation in Greece.

scholarly journals Industrial, CBD, and Wild Hemp: How Different Are Their Essential Oil Profile and Antimicrobial Activity?

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4631
Author(s):  
Valtcho D. Zheljazkov ◽  
Vladimir Sikora ◽  
Ivayla Dincheva ◽  
Miroslava Kačániová ◽  
Tess Astatkie ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Cannabis Sativa ◽  
Commercial Production ◽  
Caryophyllene Oxide ◽  
Breeding Lines ◽  
The Usa ◽  
Total Oil ◽  
Industrial Hemp ◽  
Selection Of

Hemp (Cannabis sativa L.) is currently one of the most controversial and promising crops. This study compared nine wild hemp (C. sativa spp. spontanea V.) accessions with 13 registered cultivars, eight breeding lines, and one cannabidiol (CBD) hemp strain belonging to C. sativa L. The first three groups had similar main essential oil (EO) constituents, but in different concentrations; the CBD hemp had a different EO profile. The concentration of the four major constituents in the industrial hemp lines and wild hemp accessions varied as follows: β-caryophyllene 11–22% and 15.4–29.6%; α-humulene 4.4–7.6% and 5.3–11.9%; caryophyllene oxide 8.6–13.7% and 0.2–31.2%; and humulene epoxide 2, 2.3–5.6% and 1.2–9.5%, respectively. The concentration of CBD in the EO of wild hemp varied from 6.9 to 52.4% of the total oil while CBD in the EO of the registered cultivars varied from 7.1 to 25%; CBD in the EO of the breeding lines and in the CBD strain varied from 6.4 to 25% and 7.4 to 8.8%, respectively. The concentrations of δ9-tetrahydrocannabinol (THC) in the EO of the three groups of hemp were significantly different, with the highest concentration being 3.5%. The EO of wild hemp had greater antimicrobial activity compared with the EO of registered cultivars. This is the first report to show that significant amounts of CBD could be accumulated in the EO of wild and registered cultivars of hemp following hydro-distillation. The amount of CBD in the EO can be greater than that in the EO of the USA strain used for commercial production of CBD. Furthermore, this is among the first reports that show greater antimicrobial activity of the EO of wild hemp vs. the EO of registered cultivars. The results suggest that wild hemp may offer an excellent opportunity for future breeding and the selection of cultivars with a desirable composition of the EO and possibly CBD-rich EO production.

Endophytes of industrial hemp (Cannabis sativa L.) cultivars: identification of culturable bacteria and fungi in leaves, petioles, and seeds

2018 ◽  
Vol 64 (10) ◽  
pp. 664-680 ◽  
Author(s):  
Maryanne Scott ◽  
Mamta Rani ◽  
Jamil Samsatly ◽  
Jean-Benoit Charron ◽  
Suha Jabaji
Keyword(s):  
Biochemical Characterization ◽  
Cannabis Sativa ◽  
Growth Promotion ◽  
Phylogenetic Analyses ◽  
Leaf Tissue ◽  
Cellulase Production ◽  
Culturable Bacteria ◽  
Fungal Strains ◽  
Bacteria And Fungi ◽  
Industrial Hemp

Plant endophytes are a group of microorganisms that reside asymptomatically within the healthy living tissue. The diversity and molecular and biochemical characterization of industrial hemp-associated endophytes have not been previously studied. This study explored the abundance and diversity of culturable endophytes residing in petioles, leaves, and seeds of three industrial hemp cultivars, and examined their biochemical attributes and antifungal potential. A total of 134 bacterial and 53 fungal strains were isolated from cultivars Anka, CRS-1, and Yvonne. The number of bacterial isolates was similarly distributed among the cultivars, with the majority recovered from petiole tissue. Most fungal strains originated from leaf tissue of cultivar Anka. Molecular and phylogenetic analyses grouped the endophytes into 18 bacterial and 13 fungal taxa, respectively. The most abundant bacterial genera were Pseudomonas, Pantoea, and Bacillus, and the fungal genera were Aureobasidium, Alternaria, and Cochliobolus. The presence of siderophores, cellulase production, and phosphorus solubilization were the main biochemical traits. In proof-of-concept experiments, re-inoculation of tomato roots with some endophytes confirmed their migration to aerial tissues of the plant. Taken together, this study demonstrates that industrial hemp harbours a diversity of microbial endophytes, some of which could be used in growth promotion and (or) in biological control designed experiments.

scholarly journals Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 496 ◽  
Author(s):  
Fidelia Cascini ◽  
Alessio Farcomeni ◽  
Daniele Migliorini ◽  
Laura Baldassarri ◽  
Ilaria Boschi ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Cannabis Sativa ◽  
Fiber Type ◽  
Nucleotide Polymorphisms ◽  
Loss Of Function ◽  
Single Nucleotide ◽  
Identification Process ◽  
Cannabidiolic Acid ◽  
Drug Type ◽  
Industrial Hemp

Genetic markers can be used in seeds and in plants to distinguish drug-type from fiber-type Cannabis Sativa L. varieties even at early stages, including pre-germination when cannabinoids are not accumulated yet. With this aim, this paper reports sequencing results for tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) genes from 21 C. sativa L. varieties. Taking into account that THCAS- and CBDAS-derived enzymes compete for the same substrate, the novelty of this work relies in the identification of markers based on both THCAS and CBDAS rather than THCAS alone. Notably, in our panel, we achieved an adequate degree of discrimination (AUC 100%) between drug-type and fiber-type cannabis samples. Our sequencing approach allowed identifying multiple genetic markers (single-nucleotide polymorphisms—SNPs—and a deletion/insertion) that effectively discriminate between the two subgroups of cannabis, namely fiber type vs. drug type. We identified four functional SNPs that are likely to induce decreased THCAS activity in the fiber-type cannabis plants. We also report the finding on a deletion in the CBDAS gene sequence that produces a truncated protein, possibly resulting in loss of function of the enzyme in the drug-type varieties. Chemical analyses for the actual concentration of cannabinoids confirmed the identification of drug-type rather than fiber-type genotypes. Genetic markers permit an early identification process for forensic applications while simplifying the procedures related to detection of therapeutic or industrial hemp.

scholarly journals Analysis of Phenolic Compounds in Commercial Cannabis sativa L. Inflorescences Using UHPLC-Q-Orbitrap HRMS

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 631 ◽  
Author(s):  
Luana Izzo ◽  
Luigi Castaldo ◽  
Alfonso Narváez ◽  
Giulia Graziani ◽  
Anna Gaspari ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Radical Scavenging ◽  
Mean Value ◽  
Vast Number ◽  
Health Promoting ◽  
Total Polyphenolic Content ◽  
Therapeutic Properties ◽  
Polar Extracts ◽  
Industrial Hemp ◽  
Spectrometry Measurement

Industrial hemp (Cannabis sativa L. Family Cannabaceae) contains a vast number of bioactive relevant compounds, namely polyphenols including flavonoids, phenolic acids, phenol amides, and lignanamides, well known for their therapeutic properties. Nowadays, many polyphenols-containing products made of herbal extracts are marketed, claiming to exert health-promoting effects. In this context, industrial hemp inflorescence may represent an innovative source of bioactive compounds to be used in nutraceutical formulations. The aim of this work was to provide a comprehensive analysis of the polyphenolic fraction contained in polar extracts of four different commercial cultivars (Kompoti, Tiborszallasi, Antal, and Carmagnola Cs) of hemp inflorescences through spectrophotometric (TPC, DPPH tests) and spectrometry measurement (UHPLC-Q-Orbitrap HRMS). Results highlighted a high content of cannflavin A and B in inflorescence analyzed samples, which appear to be cannabis-specific, with a mean value of 61.8 and 84.5 mg/kg, meaning a ten-to-hundred times increase compared to other parts of the plant. Among flavonols, quercetin-3-glucoside reached up to 285.9 mg/kg in the Carmagnola CS cultivar. Catechin and epicatechin were the most representative flavanols, with a mean concentration of 53.3 and 66.2 mg/kg, respectively, for all cultivars. Total polyphenolic content in inflorescence samples was quantified in the range of 10.51 to 52.58 mg GAE/g and free radical-scavenging included in the range from 27.5 to 77.6 mmol trolox/kg. Therefore, C. sativa inflorescence could be considered as a potential novel source of polyphenols intended for nutraceutical formulations.

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