Effects of a commercial inoculant of lactic acid bacteria on the composition of silages made from grasses of low dry matter content

1991 ◽  
Vol 54 (2) ◽  
pp. 177-190 ◽  
Author(s):  
Tore Nesbakken ◽  
Margrethe Broch-Due
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Dry Matter ◽  
Matter Content ◽  
Dry Matter Content ◽  
Commercial Inoculant

scholarly journals Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs

2021 ◽  
Vol 9 (7) ◽  
pp. 1346
Author(s):  
Mariana Petkova ◽  
Petya Stefanova ◽  
Velitchka Gotcheva ◽  
Angel Angelov
Keyword(s):  
Lactic Acid ◽  
Sequence Analysis ◽  
Lactic Acid Bacteria ◽  
Antimicrobial Properties ◽  
Matter Content ◽  
Starter Cultures ◽  
Rrna Gene ◽  
Dry Matter Content ◽  
Isolation And Characterization ◽  
Selection Of

Traditional sourdoughs in Bulgaria were almost extinct during the centralized food production system. However, a rapidly developing trend of sourdough revival in the country is setting the demand for increased production and use of commercial starter cultures. The selection of strains for such cultures is based on geographical specificity and beneficial technological properties. In this connection, the aim of this study was to isolate, identify and characterize lactic acid bacteria (LAB) and yeasts from typical Bulgarian sourdoughs for the selection of strains for commercial sourdough starter cultures. Twelve samples of typical Bulgarian sourdoughs were collected from different geographical locations. All samples were analyzed for pH, total titratable acidity and dry matter content. Enumeration of LAB and yeast was also carried out. Molecular identification by 16S rDNA sequence analysis was performed for 167 LAB isolates, and 106 yeast strains were identified by ITS1-5.8S-ITS2 rRNA gene partial sequence analysis. The LAB strains were characterized according to their amylolytic and proteolytic activity and acidification capacity, and 11 strains were selected for further testing of their antimicrobial properties. The strains with the most pronounced antibacterial and antifungal activity are listed as recommended candidates for the development of starter cultures for sourdoughs or other food products.

Effect of cereal and pea monocultures and combinations and silage additives on whole-crop cereal silage nutritive and fermentation characteristics

2013 ◽  
Vol 53 (5) ◽  
pp. 427 ◽  
Author(s):  
J. L. Jacobs ◽  
G. N. Ward
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Nutritive Value ◽  
Matter Content ◽  
Efficient Production ◽  
Dry Matter Content ◽  
Total N ◽  
Winter Cereals ◽  
Fermentation Parameters ◽  
Silage Additives

The efficient production and utilisation of home-grown feed is considered one of the key factors that underpins the profitability of dairy systems in southern Australia. The use of winter forage cereals for grazing and silage provides an opportunity to achieve high dry matter yields over the winter and spring period. However, questions remain on the nutritive value of whole-crop cereal silage and its subsequent use as a production feed in livestock systems. This experiment examined the nutritive characteristics of winter wheat, triticale, forage peas and bi-crops of cereals and peas sown at different proportions, cut for silage at the soft dough growth stage of the cereals and their subsequent silage nutritive characteristics and fermentation patterns when ensiled with and without bacterial inoculant additives over 2 consecutive years. The estimated metabolisable energy (ME) (Year 1) and crude protein (CP) (Years 1 and 2) concentrations of the forage pea before and after ensiling were higher (P < 0.05) than all other forages in both years. The cereal–pea mixes had similar estimated ME values to the cereal monocultures both before harvesting and as silage, although there were significant improvements in CP concentration at the higher rates of pea inclusion. All resultant silages were well fermented as indicated by low pH, low proportions of total N as ammonia-N and high lactic acid concentrations. There were marked differences in the proportions of lactic acid and acetic acid in the pea silages between years and this is likely a result of dry matter content differences at ensiling. There was no effect of silage additives on resultant silage nutritive characteristics or fermentation parameters indicating that well fermented silage can be achieved without the additional cost of using a silage additive. This study has indicated that forage peas can be ensiled with winter cereals and produce silages that have higher CP concentrations than cereal silage but with similar fermentation parameters. Furthermore, this experiment has highlighted the potential of growing a monoculture of forage peas for ensiling with the resulting silage having higher estimated ME and CP concentrations.

scholarly journals Influence of lactic acid and cellulolytic bacteria on the physicochemical parameters of alfalfa silage

2021 ◽  
Vol 901 (1) ◽  
pp. 012036
Author(s):  
A Bogdanova ◽  
A Payuta ◽  
A Alekseev ◽  
A Konovalov
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Matter Content ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Dry Matter Content ◽  
Cellulolytic Bacteria ◽  
Acid Fermentation ◽  
Alfalfa Silage ◽  
Thermophilic Streptococci

Abstract The study is aimed at studying the effect of a microbiological preparation consisting of lactic acid bacteria, thermophilic streptococci and cellulolytic bacteria on the quality of alfalfa silage with different dry matter content. Determined pH, content of organic acids, dry matter and nutrients, including carbohydrates. Inoculant application increased crude protein, crude fat and crude fiber and reduced water-soluble carbohydrates in silage. The preservation of nutrients in the test samples was higher than that of the control. pH corresponded to optimal values; however, lactic acid fermentation proceeded more intensively in the sample with an increased dry matter content.

scholarly journals Some factors affecting silage fermentation. 1. Influence of laceration and storage temperature.

1959 ◽  
Vol 7 (2) ◽  
pp. 134-137
Author(s):  
G.W. Wieringa
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Storage Temperature ◽  
Matter Content ◽  
Dry Matter Content ◽  
Factors Affecting ◽  
Silage Fermentation ◽  
Higher Temperature ◽  
Lower Temperature ◽  
And Storage

In laboratory-scale ensiling experiments [See Abs. 602] it was shown that lactic-acid bacteria developed more rapidly in silage made from crushed grass than in silage made from grass chopped into 5-cm. lengths. In further experiments using grass of high-protein and low dry-matter content, crushed and ensiled at 20 or 30 degrees C., or uncrushed grass ensiled at 30 degrees G. with 1, 2, 3 or 4% of added molasses, good silage was produced only where 4% molasses or the lower temperature was used. Butyric-acid bacteria developed best at the higher temperature.-R.B. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Effect of the different level of a dry feed additive on the lupin silage quality

2005 ◽  
Vol 53 (5) ◽  
pp. 21-30
Author(s):  
Petr Doležal ◽  
Josef Rotter ◽  
Jan Doležal ◽  
Václav Pyrochta ◽  
Jaroslav Poul
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Ph Value ◽  
Total Content ◽  
Matter Content ◽  
Feed Additive ◽  
Dry Matter Content ◽  
Higher Alcohol

In the experiment, the effect of additive on the fermentation quality of lupine was examined, by comparing with the untreated control. Fresh green Lupine (Lupines lupine), variete Juno, dry matter content 187.15 g/kg at full waxy stage of maturiy were chopped to the legth of cut ca 30–50 mm. The crop was artificially wilted for a periody 24 h and ensiled as described above. Lupine were ensiled for 98 days in laboratory silos, capacity about 4 L alone or with supplementation of feed additive (5, 10, 20, 30, 40, 50 or 70 kg/tone forage respectively). The composition and as effective substances of this silage ingredient were dry whey (30%), maize meal (40%) and dry molases (30%). The silages fermented rapidly and changes in volatile fatty acids (VFA) production (P<0.01) and in sum of acids were noted. The different supplementation of additive in our experiment conditions increased significantly (P<0.01) the dry matter content, and decreased statistically significantly (P<0.01) the pH value. The higher DM content was in all experimental silages, but the highest DM content (P<0.01) was in silage with addition of 70 kg/t (231.58Ī0.91 g/kg). All treated silages were well fermented with low levels of ammonia and pH. The different addition of this aditive increased significantly (P<0.01) the contents of lactic acid and total content of acids in comparison with control silage. The experimental silage with higher (5%, resp. 7%) feed supplementation was of better quality (significantly higher ratio LA/sum of acids, higher content of lactic acid, lower NH3 content and pH value) than the control silage, or silage with lower concentration. In experiment feed additive–treated silages (by groups of 5 or 50 and 70 kg/t) had significantly higher alcohol content than untreated silage. It was concluded that feed additive used as a silage additive improved fermentation of lupine, reduced acetic acid and ammonia production and increased silage nutritive value.

scholarly journals Maize for silage: I. Conservation of whole maize plant for silage with treatment of preservatives and urea before ensiling

1979 ◽  
Vol 51 (1) ◽  
pp. 229-237
Author(s):  
J. Setälä ◽  
J. Seppälä ◽  
S. Pulli
Keyword(s):  
Lactic Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Sugar Content ◽  
Matter Content ◽  
Dry Matter Content ◽  
Crude Protein Content ◽  
Fresh Weight ◽  
Fermentation Rate

Maize was harvested with a precision chopper and ensiled in five plastic tower silos, 1 500 kg fodder/silo. The silages and the ensiling methods were: 0.5 % urea per fresh weight (A), 1.0 % urea per fresh weight + Viher-acid 4 1/1000 kg of silage (B), 0.5 % urea per fresh weight + Viher-acid 4 1/1000 kg of silage (C). Viher-acid 4 1/1000 kg of silage (D) and silage without urea or preservatives (E). The dry matter content of the whole maize was 21 % and the crude protein content was 10.8 % in DM. The addition of urea increased the crude protein (N x 6.25) content in A- and C-silages to 17.7 percent and in B-silage to 24.9 percent. The quality of the silages was good, and some marks of butyric acid was found only in the silage ensiled without Viher-acid or urea. The addition of Viher-acid lowered the fermentation rate so that the sugar content of the corresponding silages was higher. The sugar content was higher (P < 0.01) and the content of lactic acid lower (P < 0.001) in the silage ensiled only with Viher-acid. Urea increased the content of lactic acid and the content of acetic acid of the silages. In the silages ensiled with urea the use of Viher-acid tended to lower the fermentation rate. The use of urea increased the NH3-N (P < 0.05) and the soluble N contents (P < 0.001) in the silages. In spite of the low dry matter content of the silages some enrichment of the urea in the lower parts of the silos was only found in the silage ensiled with 1 % of urea (P > 0.05) and Viher-acid. This was due to the larger effluent amount from the silo. The fermentation losses of dry matter were smallest in the silage D (3.9 %) and highest in the silage E (11.6 %) (P < 0.05). The losses in A-, B- and C-silages were 7.6 %, 4.7 % and 9.2 %, respectively. The use of urea decreased the fermentation losses.

Optimum harvest date of maize for biogas and silage purposes

2009 ◽  
Vol 57 (2) ◽  
pp. 119-125
Author(s):  
G. Hadi
Keyword(s):  
Dry Matter ◽  
Biogas Production ◽  
Weather Conditions ◽  
Vegetation Period ◽  
Dry Mass ◽  
Matter Content ◽  
Harvest Date ◽  
Dry Matter Content ◽  
Dry Matter Yield ◽  
Vegetative Organs

The dry matter and moisture contents of the aboveground vegetative organs and kernels of four maize hybrids were studied in Martonvásár at five harvest dates, with four replications per hybrid. The dry matter yield per hectare of the kernels and other plant organs were investigated in order to obtain data on the optimum date of harvest for the purposes of biogas and silage production.It was found that the dry mass of the aboveground vegetative organs, both individually and in total, did not increase after silking. During the last third of the ripening period, however, a significant reduction in the dry matter content was sometimes observed as a function of the length of the vegetation period. The data suggest that, with the exception of extreme weather conditions or an extremely long vegetation period, the maximum dry matter yield could be expected to range from 22–42%, depending on the vegetation period of the variety. The harvest date should be chosen to give a kernel moisture content of above 35% for biogas production and below 35% for silage production. In this phenophase most varieties mature when the stalks are still green, so it is unlikely that transport costs can be reduced by waiting for the vegetative mass to dry.

Persistence of modern varieties and hybrids of carrots and its dependence on the biochemical composition

2020 ◽  
pp. 16-19
Author(s):  
Е.В. Янченко
Keyword(s):  
Botrytis Cinerea ◽  
Sclerotinia Sclerotiorum ◽  
Dry Matter ◽  
Inverse Correlation ◽  
Dry Weight ◽  
Matter Content ◽  
White Rot ◽  
Dry Matter Content ◽  
Important Indicator ◽  
Modern Varieties

Цель исследований – дать оценку сохраняемости и болезнеустойчивости современных сортов и гибридов моркови столовой и определить корреляционные зависимости влияния биохимических показателей качества на сохраняемость и степень поражения моркови столовой различными видами болезней в процессе хранения. Исследования проводились в 2011–2016 годах во ВНИИО – филиале ФГБНУ ФНЦО по общепринятым методикам. В биохимической лаборатории отдела земледелия и агрохимии содержание сухого вещества определяли высушиванием до абсолютно сухого веса, общего сахара – по Бертрану, аскорбиновой кислоты – по Мурри, нитраты – ионоселективным методом. При характеристике моркови столовой важнейший показатель, определяющий его качество – количество сухого вещества и сахаров. В процессе хранения были выявлены следующие болезни моркови: серая гниль (Botrytis cinerea Pers. ex Fr.), белая гниль (Sclerotinia sclerotiorum (Lib.)), белая парша (Rhizoctonia carotae Rad.), альтернариоз (Alternaria radicina M., Dr. et E.). В большей степени сортообразцы моркови столовой поражались серой гнилью. Лучшими по сохраняемости сортообразцами были Корсар (94,6%), F1Берлин (94,5%), Берликум Роял (94,1%) и F1 Звезда (94%). Сохраняемость у зарубежных сортов и гибридов моркови столовой была немного выше, чем у отечественных (на 0,4%) как за счет меньшей величины убыли массы (6,3% против 6,4%), так и потерь от болезней (1,6% против 1,9%). Сохраняемость корнеплодов моркови находится в прямой корреляционной зависимости от содержания сухого вещества (r=+0,41), каротиноидов (r=+0,39), моносахаров (r­=+0,30) и суммы сахаров (r=+0,27). Проявление серой гнили находится в обратной корреляционной связи с содержанием сухого вещества и каротиноидов (r=-0,37 и r=-0,35 соответственно), белой парши – в прямой корреляции с содержанием сухого вещества , моносахаров и дисахаров (r= +0,21; r= +0,39; r= -0,41 соответственно), белой гнили в обратной корреляционной связи с содержанием сухого вещества, моносахаров и дисахаров. The purpose of the research is to assess the persistence and disease resistance of modern varieties and hybrids of carrots and to determine the correlation between the influence of biochemical quality indicators on the persistence and degree of damage to carrots by various types of diseases during storage. The research was conducted in 2011–2016 at ARRIVG – branch of FSBSI FSVC, according to generally accepted methods. In the biochemical laboratory of the Department of Agriculture and Agrochemistry, the dry matter content was determined by drying to absolutely dry weight, total sugar – by Bertran, ascorbic acid – by Murri, nitrates – by the ion-selective method. When describing carrots, the most important indicator that determines its quality is the amount of dry matter and sugars. During storage, the following diseases of carrots were detected: gray rot (Botrytis cinerea Pers. ex Fr.), white rot (Sclerotinia sclerotiorum (Lib.), white scab (Rhizoctonia carotae Rad.), alternariasis (Alternaria radicina M., Dr. et E.). To a greater extent, varieties of table carrots were affected by gray rot. The best preserved varieties were Corsar (94.6%), F1 Berlin (94.5%), Berlicum Royal (94.1%) and F1 Zvezda (94%). The persistence of foreign varieties and hybrids of table carrots was slightly higher than that of domestic ones by 0.4%. both due to a smaller amount of weight loss (6.3% vs. 6.4%) and losses from diseases (1.6% vs. 1.9%). The persistence of carrot root crops is directly correlated with the content of dry matter (r=+0.41), carotenoids (r=+0.39), monosaccharides (r=+0.30) and the amount of sugars (r=+0.27). The manifestation of gray rot is in inverse correlation with the content of dry matter and carotenoids (r=-0.37 and r=-0.35, respectively), white scab is in direct correlation with the content of dry matter (r= +0.21; r= +0.39; r= –0.41, respectively), white rot is in inverse correlation with the content of dry matter, monosaccharides and disaccharides.

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