Injection of a microtextured polymer part surface: Influence of experimental parameters on the replication rate

The role of calcium in modulating cellular replication and differentiation has been described for various cell types. In the present study, the effects of Ca++ on the growth and differentiation of cultured rat esophageal epithelial cells was investigated.Epithelial cells were isolated from esophagi taken from 8 week-old male CDF rats by the enzymatic dissociation method of Kaighn. The cells were cultured in PFMR-4 medium supplemented with 0.25 mg/ml dialyzed fetal bovine serum, 5 ng/ml epidermal growth factor, 10-6 M hydrocortisone 10-6 M phosphoethanolamine, 10-6 M ethanolamine, 5 pg/ml insulin, 5 ng/ml transferrin, 10 ng/ml cholera toxin and 50 ng/ml garamycin at 36.5°C in a humidified atmosphere of 3% CO2 in air. At weekly intervals, the cells were subcultured with a solution containing 1% polyvinylpyrrolidone, 0.01% EGTA, and 0.05% trypsin. After various passages, the replication rate of the cells in PFMR-4 medium containing from 10-6 M to 10-3 M Ca++ was determined using a clonal growth assay.

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The use of insoluble matter of Moroccan oil shale for removal of dyes from aqueous solution

10.31221/osf.io/x4v9p ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Methylene Blue ◽

Oil Shale ◽

Heating Temperature ◽

Blue Dye ◽

Adsorption Capacities ◽

Experimental Parameters ◽

Initial Ph ◽

New Material ◽

Activating Agent ◽

Moderate Cost

The study aims to use an adsorbent natural based of Moroccan oil shale of Timahdit area (Y layer) in a physical-chemical adsorption process for treating industrial discharges colorful. The used adsorbent is the insoluble party of the sub-critical extraction of decarbonized oil shale of Timahdit. The tests performed on the methylene blue (MB), showed a strong elimination in the first 10 minutes. The influences of various experimental parameters were studied: mass ratio of adsorbent, time and temperature of thermal treatment, contact time, pH of MB and heating temperature of solution on the parameters of material were studied. The experimental results have shown that the adsorption of methylene blue dye by the adsorbent is more than 90% at initial pH a range 6-7 at room temperature for 30 minutes. The process is simple and the adsorbent produced is a new material with interesting adsorption capacities of moderate cost which does not require an activating agent and can be used as industrial adsorbent for the decontamination of effluents containing organic pollutants.

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Determination of the minimum number of technological bases of a part

10.36652/0042-4633-2020-9-73-75 ◽

2020 ◽

pp. 73-75

Author(s):

B.M. Bazrov ◽

T.M. Gaynutdinov

Keyword(s):

Cost Price ◽

Labor Input ◽

Part Surface ◽

Technological Base ◽

Size Accuracy ◽

Minimum Number ◽

The Cost ◽

Input Cost ◽

Selection Of

The selection of technological bases is considered before the choice of the type of billet and the development of the route of the technological process. A technique is proposed for selecting the minimum number of sets of technological bases according to the criterion of equality in the cost price of manufacturing the part according to the principle of unity and combination of bases at this stage. Keywords: part, surface, coordinating size, accuracy, design and technological base, labor input, cost price. [email protected]

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Optimization of Extraction Parameters of Reverse Iontophoretic Determination of Blood Glucose in an Artificial Skin Model

Current Analytical Chemistry ◽

10.2174/1573411015666190710232858 ◽

2020 ◽

Vol 16 (6) ◽

pp. 722-737

Author(s):

Cigdem Yengin ◽

Emrah Kilinc ◽

Fatma Gulay Der ◽

Mehmet Can Sezgin ◽

Ilayda Alcin

Keyword(s):

Ionic Strength ◽

Body Fluids ◽

Extraction Time ◽

Artificial Skin ◽

Dialysis Membrane ◽

Skin Model ◽

Non Invasive ◽

Electrode Distance ◽

Experimental Parameters

Background: Reverse İontophoresis (RI) is one of the promising non-invasive technologies. It relies on the transition of low magnitude current through the skin and thus glucose measurement becomes possible as it is extracted from the surface during this porter current flow. Objective: This paper deals with the development and optimization of an RI determination method for glucose. CE dialysis membrane based artificial skin model was developed and the dependence of RI extraction on various experimental parameters was investigated. Method: Dependence of RI extraction performance on noble electrodes (platinum, silver, palladium, ruthenium, rhodium) was checked with CA, CV and DPV, in a wide pH and ionic strength range. Optimizations on inter-electrode distance, potential type and magnitude, extraction time, gel type, membrane MWCO, usage frequency, pretreatment, artificial body fluids were performed. Results: According to the optimized results, the inter-electrode distance was 7.0 mm and silver was the optimum noble metal. Optimum pH and ionic strength were achieved with 0.05M PBS at pH 7.4. Higher glucose yields were obtained with DPV, while CA and CV achieved almost the same levels. During CA, +0.5V achieved the highest glucose yield and higher potential even caused a decrease. Glucose levels could be monitored for 24 hours. CMC gel was the optimum collection media. Pretreated CE membrane with 12kD MWCO was the artificial skin model. Pretreatment affected the yields while its condition caused no significant difference. Except PBS solution (simulated as artificial plasma), among the various artificial simulated body fluids, intestinal juice formulation (AI) and urine formulation U2 were the optimum extraction media, respectively. Conclusion: In this study, various experimental parameters (pretereatment procedure, type and MWCO values of membranes, inter-electrode distance, electrode material, extraction medium solvents, ionic strength and pH, collection medium gel type, extraction potential type and magnitude, extraction time and etc) were optimized for the non-invasive RI determination of glucose in a CE dialysis membrane-based artificial skin model and various simulated artificial body fluids.

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Effect of Experimental Parameters on Wear Response of Thermally Sprayed Carbide Based Coatings

Materials Research ◽

10.1590/1980-5373-mr-2018-0475 ◽

2018 ◽

Vol 22 (1) ◽

Cited By ~ 4

Author(s):

Nitesh Vashishtha ◽

Sanjay Sapate

Keyword(s):

Experimental Parameters ◽

Wear Response ◽

Thermally Sprayed

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Laser Floating Zone Growth: Overview, Singular Materials, Broad Applications, and Future Perspectives

Crystals ◽

10.3390/cryst11010038 ◽

2020 ◽

Vol 11 (1) ◽

pp. 38

Author(s):

Francisco Rey-García ◽

Rafael Ibáñez ◽

Luis Alberto Angurel ◽

Florinda M. Costa ◽

Germán F. de la Fuente

Keyword(s):

Equilibrium Phase ◽

Polycrystalline Materials ◽

Floating Zone ◽

High Crystalline Quality ◽

Experimental Parameters ◽

Phase Selectivity ◽

Oriented Polycrystalline ◽

Laser Heated ◽

Lfz Technique ◽

Zone Growth

The Laser Floating Zone (LFZ) technique, also known as Laser-Heated Pedestal Growth (LHPG), has been developed throughout the last several decades as a simple, fast, and crucible-free method for growing high-crystalline-quality materials, particularly when compared to the more conventional Verneuil, Bridgman–Stockbarger, and Czochralski methods. Multiple worldwide efforts have, over the years, enabled the growth of highly oriented polycrystalline and single-crystal high-melting materials. This work attempted to critically review the most representative advancements in LFZ apparatus and experimental parameters that enable the growth of high-quality polycrystalline materials and single crystals, along with the most commonly produced materials and their relevant physical properties. Emphasis will be given to materials for photonics and optics, as well as for electrical applications, particularly superconducting and thermoelectric materials, and to the growth of metastable phases. Concomitantly, an analysis was carried out on how LFZ may contribute to further understanding equilibrium vs. non-equilibrium phase selectivity, as well as its potential to achieve or contribute to future developments in the growth of crystals for emerging applications.

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Synthetic routes to manganese oxoborate and correlations between experimental parameters and properties

Ceramics International ◽

10.1016/j.ceramint.2021.03.049 ◽

2021 ◽

Author(s):

O. Icten ◽

G. Ozgenc ◽

D. Ozer ◽

D.A. Kose ◽

G. Elmaci ◽

...

Keyword(s):

Experimental Parameters ◽

Synthetic Routes

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Device-independent quantum key distribution with random key basis

Nature Communications ◽

10.1038/s41467-021-23147-3 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

René Schwonnek ◽

Koon Tong Goh ◽

Ignatius W. Primaatmaja ◽

Ernest Y.-Z. Tan ◽

Ramona Wolf ◽

...

Keyword(s):

Quantum Key Distribution ◽

Bell Inequality ◽

Key Distribution ◽

Theory And Practice ◽

Information Theoretic ◽

Information Theoretic Security ◽

Experimental Parameters ◽

Secret Keys ◽

Simple Variant ◽

First Time

AbstractDevice-independent quantum key distribution (DIQKD) is the art of using untrusted devices to distribute secret keys in an insecure network. It thus represents the ultimate form of cryptography, offering not only information-theoretic security against channel attacks, but also against attacks exploiting implementation loopholes. In recent years, much progress has been made towards realising the first DIQKD experiments, but current proposals are just out of reach of today’s loophole-free Bell experiments. Here, we significantly narrow the gap between the theory and practice of DIQKD with a simple variant of the original protocol based on the celebrated Clauser-Horne-Shimony-Holt (CHSH) Bell inequality. By using two randomly chosen key generating bases instead of one, we show that our protocol significantly improves over the original DIQKD protocol, enabling positive keys in the high noise regime for the first time. We also compute the finite-key security of the protocol for general attacks, showing that approximately 108–1010 measurement rounds are needed to achieve positive rates using state-of-the-art experimental parameters. Our proposed DIQKD protocol thus represents a highly promising path towards the first realisation of DIQKD in practice.

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Signatures of the Carrier Envelope Phase in Nonlinear Thomson Scattering

Crystals ◽

10.3390/cryst11050528 ◽

2021 ◽

Vol 11 (5) ◽

pp. 528

Author(s):

Marcel Ruijter ◽

Vittoria Petrillo ◽

Thomas C. Teter ◽

Maksim Valialshchikov ◽

Sergey Rykovanov

Keyword(s):

Laser Pulse ◽

Radiation Spectrum ◽

Thomson Scattering ◽

High Energy ◽

Phase Changes ◽

High Energy Radiation ◽

Carrier Envelope Phase ◽

High Intensity Laser ◽

Experimental Parameters ◽

Intensity Laser

High-energy radiation can be generated by colliding a relativistic electron bunch with a high-intensity laser pulse—a process known as Thomson scattering. In the nonlinear regime the emitted radiation contains harmonics. For a laser pulse whose length is comparable to its wavelength, the carrier envelope phase changes the behavior of the motion of the electron and therefore the radiation spectrum. Here we show theoretically and numerically the dependency of the spectrum on the intensity of the laser and the carrier envelope phase. Additionally, we also discuss what experimental parameters are required to measure the effects for a beamed pulse.

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Investigation on the La Replacement and Little Additive Modification of High-Performance Permanent Magnetic Strontium-Ferrite

Processes ◽

10.3390/pr9061034 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1034

Author(s):

Ching-Chien Huang ◽

Chin-Chieh Mo ◽

Guan-Ming Chen ◽

Hsiao-Hsuan Hsu ◽

Guo-Jiun Shu

Keyword(s):

High Performance ◽

Single Phase ◽

Permanent Magnets ◽

High Efficiency ◽

Cobalt Content ◽

Preparation Condition ◽

Milling Process ◽

Experimental Parameters ◽

La Substitution ◽

Hard Magnets

In this work, an experiment was carried out to investigate the preparation condition of anisotropic, Fe-deficient, M-type Sr ferrite with optimum magnetic and physical properties by changing experimental parameters, such as the La substitution amount and little additive modification during fine milling process. The compositions of the calcined ferrites were chosen according to the stoichiometry LaxSr1-xFe12-2xO19, where M-type single-phase calcined powder was synthesized with a composition of x = 0.30. The effect of CaCO3, SiO2, and Co3O4 inter-additives on the Sr ferrite was also discussed in order to obtain low-temperature sintered magnets. The magnetic properties of Br = 4608 Gauss, bHc = 3650 Oe, iHc = 3765 Oe, and (BH)max = 5.23 MGOe were obtained for Sr ferrite hard magnets with low cobalt content at 1.7 wt%, which will eventually be used as high-end permanent magnets for the high-efficiency motor application in automobiles with Br > 4600 ± 50 G and iHc > 3600 ± 50 Oe.

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