Effects of soybean ethanol extract on the cell survival and oxidative stress in osteoblastic cells

Objective: This study reports protective effect of Abelmoschus moschatus seed extract against sodium fluoride-induced neurodegeneration through oxidative stress, neurohistological, and behavioral observations in Wistar rats.Methods: A total of 20 Wistar rats (around 250 g) were randomly classified into four groups, namely, control, fluoride (NaF), fluoride + A. moschatus seed aqueous extract (AMAE), and fluoride + A. moschatus seed ethanol extract (AMEE). The control group animals received normal tap water, fluoride group received fluoridated water at the rate of 40 mg/kg b. wt., 3rd group rats treated with fluoride (40 mg/kg b. wt.) + AMAE (300 mg/kg b. wt.), and 4th group rats treated with fluoride (40 mg/kg b. wt.) + AMEE (300 mg/kg b. wt.). Neurobehavioral responses of rotarod, hot plate, and maze learning tests and oxidantive stress markers including lipid peroxidation (LPO), GSH levels, superoxide dismutase, CAT, and GSH peroxidase (GPx) activities, and also histology with H and E as well as congo red staining were studied in control, fluoride, and A. moschatus seed extract treated against fluoride groups.Results: Decreased neurobehavioral responses with rotarod, hot plate, and maze and enhanced LPO (p<0.05) levels were found in fluoride received animals. Whereas, the superoxide dismutase (SOD), CAT, GSH, and GPx were decreased (p<0.05) in NaF treatment. The rats received seed extract along with NaF showed significant reversal of behavioral and oxidative stress markers and the effect of ethanol extract was more pronounced than aqueous extract. The fluoride-treated group showed disturbed cell structure and reduced number of cells in H and E as well as congo red staining which was reversed in cell morphology and restored cell number in seed extract against NaF-treated group. As a result of increased LPO, decreased antioxidant system, and decreased number of cells, neurodegeneration was observed resulting in the disturbance in functions associated with reported behavior.Conclusion: Okra with high antioxidants activity, seed extract showed reversal of LPO levels and antioxidant status in the brain tissue. And also plant extract administered rats displayed normal cell structure and number of cells than only fluoride received group. Therefore, the aqueous and ethanolic extract of A. moschatus plant seeds has neuroprotective effects against fluoride-induced motor, nociceptive, learning behavior, and on histological structure of brain through antioxidant mechanism. The ethanol extract has shown more efficacy than aqueous extract.

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Protective effects of ethanolic extract of Alhagi maurorum roots on renal failure induced by acetaminophen in mice

Journal of Applied Biotechnology & Bioengineering ◽

10.15406/jabb.2021.08.00246 ◽

2021 ◽

Vol 8 (1) ◽

pp. 16-22

Author(s):

Seham I AL-Nafea ◽

Mohammed O Aljahdali

Keyword(s):

Oxidative Stress ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Serum Levels ◽

High Dose ◽

Root Extract ◽

Protective Effects ◽

Oxidative Stress Biomarkers ◽

Protective Actions ◽

And Oxidative Stress

The protective actions of ethanol Alhagi maurorum (AM) root ethanol extract on acetaminophen-induced oxidative stress and renal toxicity in mice was evaluated. Forty male SWR strain albino mice aged 8 weeks were grouped into five groups. G1 (n=5): as control. G2 (n=5): administered orally a single dose of acetaminophen (2000mg/kg). G3 (n=10) administrated orally 200 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day and; G4 (n=10) administrated orally 400 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day; G5 (n=10) administrated orally 600 mg/kg of roots ethanol extract for one week then acetaminophen as G2 at 8th day. At end of experiments, the mice were killed under anesthesia and blood samples were gathered to preform complete blood test (CBC), serum levels of urea and creatinine and oxidative stress biomarkers as superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) using available Elisa mice kits. Kidneys were removed and histologically examined. Acetaminophen intake significantly elevated WBCs, neutrophils, monocytes, urea and creatinine levels and significantly decreased RBCs, hemoglobin, hematocrit, GSH, SOD and CAT (P <0.05). Treatment with Alhagi maurorum roots extract especially high dose (600 mg/kg) resulted in decreased in WBCs, neutrophils, monocytes, urea and creatinine levels and significantly increased RBCs, hemoglobin, hematocrit, GSH, SOD and CATversusacetaminophen group. Alhagi maurorum root extract treatment similarly decreased renal histological alteration induced by acetaminophen. This study can be utilized as prove of reading that Alhagi maurorum ethanol root extract especially high dose might be administered to prevent renal destruction induced by acetaminophen due to its antioxidant activity

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Effects of an ethanol extract fromLactobacillus paracaseisubsp.paracaseiNTU 101 fermented skimmed milk on lipopolysaccharide-induced periodontal inflammation in rats

Food & Function ◽

10.1039/c8fo01303a ◽

2018 ◽

Vol 9 (9) ◽

pp. 4916-4925 ◽

Cited By ~ 2

Author(s):

Te-Hua Liu ◽

Tsung-Yu Tsai ◽

Tzu-Ming Pan

Keyword(s):

Oxidative Stress ◽

Periodontal Disease ◽

Inflammatory Cytokine ◽

Alveolar Bone ◽

Ethanol Extract ◽

Alveolar Bone Loss ◽

Periodontal Inflammation ◽

Cytokine Levels ◽

Skimmed Milk ◽

And Oxidative Stress

NTU 101-fermented skimmed milk ethanol extract (NTU101FMEE) decreased pro-inflammatory cytokine levels and oxidative stress in the gingival tissues and serum of periodontal disease rat. NTU101FMEE inhibited alveolar bone loss induced by periodontal disease.

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Oxidative stress induces cell death partially by decreasing both mRNA and protein levels of nicotinamide phosphoribosyltransferase in differentiated PC12 cells

PeerJ ◽

10.7717/peerj.11401 ◽

2021 ◽

Vol 9 ◽

pp. e11401

Author(s):

Cuiyan Zhou ◽

Weihai Ying

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

Cell Survival ◽

Pc12 Cells ◽

Neurological Diseases ◽

Biological Effects ◽

Stress Conditions ◽

Nicotinamide Phosphoribosyltransferase ◽

Differentiated Pc12 Cells ◽

And Oxidative Stress

Background. Multiple studies have indicated crucial roles of NAD+ deficiency in several neurological diseases and aging. It is critical to discover the mechanisms underlying the NAD+ deficiency. A decreased level of Nicotinamide phosphoribosyltransferase (Nampt)—an important enzyme in the salvage pathway of NAD+ synthesis—has been found under certain pathological conditions, while the mechanisms underlying the Nampt decrease are unclear. The purpose of this study is to test the hypothesis that oxidative stress can produce decreased Nampt, and to investigate the biological effects of Nampt on NAD+ synthesis and cell survival under both basal and oxidative stress conditions. Methods. We used differentiated PC12 cells as a cellular model to investigate the effects of oxidative stress on the levels of Nampt. Multiple assays, including flow cytometry-based cell death assays and NAD+ assays were conducted. Results. First, oxidative stress can decrease the levels of Nampt mRNA and Nampt protein; second, Nampt plays significant roles in NAD+ synthesis under both basal conditions and oxidative stress conditions; third, Nampt plays critical roles in cell survival under both basal conditions and oxidative stress conditions; and fourth, oxidative stress produced decreased NAD+ levels and cell survival partially by decreasing Nampt. Collectively, our study has indicated that oxidative stress is a pathological factor leading to decreased Nampt, which plays important roles in oxidative stress-produced decreases in NAD+ levels and cell survival. Our findings have indicated major roles of Nampt in maintaining NAD+ levels and cell survival under both basal and oxidative stress conditions.

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Antitumoral Activity of Sorafenib in Hepatocellular Carcinoma: Effects on Cell Survival and Death Pathways, Cell Metabolism Reprogramming, and Nitrosative and Oxidative Stress

Critical Reviews™ in Oncogenesis ◽

10.1615/critrevoncog.2017021302 ◽

2016 ◽

Vol 21 (5-6) ◽

pp. 413-432 ◽

Cited By ~ 2

Author(s):

Francisco J. Molina-Ruiz ◽

Raul Gonzalez ◽

Maria A. Rodriguez-Hernandez ◽

Elena Navarro-Villaran ◽

Francisco J. Padillo ◽

...

Keyword(s):

Oxidative Stress ◽

Hepatocellular Carcinoma ◽

Cell Survival ◽

Cell Metabolism ◽

Antitumoral Activity ◽

And Oxidative Stress

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Protective Effect of JXT Ethanol Extract on Radiation-Induced Hematopoietic Alteration and Oxidative Stress in the Liver

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/9017835 ◽

2018 ◽

Vol 2018 ◽

pp. 1-12

Author(s):

Xian-Zhe Dong ◽

Yu-Ning Wang ◽

Xiao Tan ◽

Ping Liu ◽

Dai-Hong Guo ◽

...

Keyword(s):

Oxidative Stress ◽

Bone Marrow ◽

Oral Administration ◽

Cell Viability ◽

Liver Tissue ◽

Bone Marrow Cells ◽

Ethanol Extract ◽

Bone Marrow Tissue ◽

Radiation Induced ◽

And Oxidative Stress

This study aims at investigating the radioprotective effect of ethanol extract from Ji-Xue-Teng (JXT,Spatholobus suberectus) on radiation-induced hematopoietic alteration and oxidative stress in the liver. Mice were exposed to a single acuteγ-radiation for the whole body at the dose of 6.0 Gy, then subjected to administration of amifostine (45 mg/kg) or JXT (40 g crude drug/kg) once a day for 28 consecutive days, respectively. Bone marrow cells and hemogram including white cells, red cells, platelet counts, and hemoglobin level were examined. The protein expression levels of pJAK2/JAK2, pSTAT5a/STAT5a, pSTAT5b/STAT5b, and Bcl-2 in bone marrow tissue; levels of reactive oxygen species (ROS); and the activity of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) in serum and liver tissue were determined. At the end of the experiment, the effect of JXT on cell viability and G-CSF and G-CSFR levels in NFS-60 cells were tested by CCK-8 assay, ELISA, and flow cytometry. The results showed that the mice exposed toγ-radiation alone exhibited a typical hematopoietic syndrome. In contrast, at the end of the 28-day experiment, irradiated mice subjected to oral administration of JXT showed an obvious improvement on blood profile with reduced leucopenia, thrombocytopenia (platelet counts), RBC, and hemoglobin levels, as well as bone marrow cells. The expression of pJAK2/JAK2, pSTAT5a/STAT5a, and Bcl-2 in bone marrow tissue was increased after JXT treatment. The elevation of ROS was due to radiation-induced toxicity, but JXT significantly reduced the ROS level in serum and liver tissue, elevated endogenous SOD and GSH-PX levels, and reduced the MDA level in the liver. JXT could also increase cell viability and G-CSFR level in NFS-60 cells, which was similar to exogenous G-CSF. Our findings suggested that oral administration of JXT effectively facilitated the recovery of hematopoietic bone marrow damage and oxidative stress of the mice induced byγ-radiation.

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