Antiinflammatory activity of methanol extract isolated from stem bark ofMagnolia kobus

2008 ◽  
Vol 22 (7) ◽  
pp. 883-888 ◽  
Author(s):  
Jong Soon Kang ◽  
Ki Hoon Lee ◽  
Mi Hwa Han ◽  
Hyunju Lee ◽  
Ji-Mi Ahn ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Antiinflammatory Activity ◽  
Stem Bark

scholarly journals GALLIC ACID: A PHENOLIC ACID AND ITS ANTIOXIDANT ACTIVITY FROM STEM BARK OF CHLOROFORM EXTRACTS OF SYZYGIUM LITORALE (BLUME) AMSHOFF (MYRTACEAE)

Molekul ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. 180 ◽  
Author(s):  
Tukiran Tukiran ◽  
Fitriyatul Mahmudah ◽  
Nurul Hidayati ◽  
Kuniyoshi Shimizu
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
Methanol Extract ◽  
Authentic Sample ◽  
Stem Bark ◽  
Spectroscopic Studies ◽  
Chloroform Fraction ◽  
Ic50 Value ◽  
Isolated Compound

A phenolic acid had been isolated from chloroform soluble fractions of a methanol extract of stem bark of Syzygium litorale, Fam. Myrtaceae. The structure of the isolated compound was elucidated and established as gallic acid through extensive spectroscopic studies (UV-Vis, FTIR, and NMR) and by comparison with literature data and authentic sample. This is the first report of the isolation of compound from this plant, although it has previously been found in Myrtaceae family such as S. aromaticum, S. cumini, S. polyanthum, S. cordatum, etc. The chloroform fraction, isolated compound, and vitamin C showed very strong antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) with IC50 value of 23.2, 7.5, and 12.5 mg/mL, respectively.

scholarly journals Pharmacological potential of methanol extract of Anacardium occidentale stem bark on alloxan-induced diabetic rats

2018 ◽  
Vol 5 (7) ◽  
pp. 2440-2454
Author(s):  
D. A. Omoboyowa ◽  
F. O. Afolabi ◽  
T. C. Aribigbola
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Methanol Extract ◽  
Stem Bark ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Β Cells

Background: The anti-hyperglycemic potential of methanol stem bark extract of Anacardium occidentale (MSBEAO) was investigated using an alloxan-induced diabetic rat model. Alloxan administration induces the generation of free radicals which can affect antioxidant status resulting in the disruption of the β-cells of the pancreas. Therefore, this study examines the antioxidant potential of the plant extract and the ameliorating effect on the pancreas of alloxan-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of 150 mg/kg body weight of alloxan monohydrate. MSBEAO, at a concentration of 100 or 200 mg/kg b.w. was orally administered to alloxan-induced diabetic rats and normal rats. The hypoglycemic effect, oral glucose tolerance test, and biochemical assay of alloxan-induced diabetic rats were assayed using standard procedures. Results: Preliminary phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, terpenoids, carbohydrates, and phenols at moderate concentrations. The lethality dose (LD50) of the plant extract was found to be equal to or less than 5000 mg/kg b.w. The hypoglycemic effect of the extract on the non-diabetic rats revealed a significant (p<0.05) decrease in the blood glucose concentration of animals administered with 1 g/kg b.w. of the extract, compared to normal control rats administered with normal saline. In the oral glucose tolerance test, the methanol extract exerted the highest response, similar to glibenclamide after 15 and 30 minutes of administration, compared to the control rats. The methanol extract yielded the highest blood glucose lowering effects after 9 days of treatment (p<0.05), compared to diabetic rats administered with normal saline and 0.3 mg/kg b.w. of glibenclamide. Administration of the extract at 200 mg/kg b.w. showed improved pancreas architecture and regeneration of the β-cells, compared with the pancreas of animals in the other groups. Conclusion: The results of this study suggest that MSBEAO is a potentially effective agent for the management of diabetes which might result from the antioxidant-generating capacity of the stem bark.

scholarly journals THE PHYTOCHEMICAL SCREENING HEXANE AND METHANOL EXTRACT OF SINYO NAKAL (DURANTA REPENS)

2020 ◽  
pp. 196-200
Author(s):  
PURWANTININGSIH SUGITA ◽  
RIZKI AMILIA ◽  
BUDI ARIFIN ◽  
DYAH UTAMI CAHYANING RAHAYU ◽  
HANHAN DIANHAR
Keyword(s):  
Fatty Acids ◽  
Developing Countries ◽  
Linoleic Acid ◽  
Vitamin E ◽  
Methanol Extract ◽  
Stem Bark ◽  
Unsaponifiable Fraction ◽  
Phytochemical Screening ◽  
Phytochemical Profile ◽  
Based Medicine

Objective: Phytochemicals are naturally present in every part of plants range from leaves, stem bark, and fruits to roots. The use of plant-based medicine is popular among individuals and communities in developing countries. This study evaluated that the phytochemical profile of Duranta repens fruits was collected from Jombang, East Java, Indonesia. Methods: Air-dried D. repens fruits were macerated by n-hexane and methanol, a separate flask. n-Hexane extract, then underwent saponification to remove fat. Then, the unsaponifiable of n-hexane and methanol extract was tested through phytochemical screening, respectively. Results: The methanol extract showed the presence of alkaloids, flavonoids, tannins, and steroids, but the unsaponifiable fraction only contained steroids. The unsaponifiable fraction was found to be a mixture of hydrocarbons ranging from C13-C20, fatty acids (palmitic and linoleic acid), squalene, and Vitamin E. Conclusion: The phytochemical screening of the plant plays an important role for pharmaceutical studies especially discovering new potential drugs for the treatment of various diseases.

Anti-Snake Venom, Anti-Arthritic and Cytotoxic Activities of Tectona grandis L. f. Stem Bark (Lamiaceae)

2020 ◽  
Vol 35 (2) ◽  
pp. 87-94
Author(s):  
Adebayo Gbolade ◽  
Oluwasegun Adedokun ◽  
Ogochukwu Ume ◽  
Johnkennedy Onyechege ◽  
Cynthia Mkparu
Keyword(s):  
Snake Venom ◽  
Methanol Extract ◽  
Tectona Grandis ◽  
Stem Bark ◽  
Cytotoxic Activities ◽  
Standard Drug ◽  
Snake Bites ◽  
Vacuum Liquid Chromatography ◽  
Proteinase Inhibition

Tectona grandis L. f. (Lamiaceae) is famous for timber production and has been used in traditional medicine for treating bronchitis, liver-related  roubles, urinary discharge amongst other diseases. Methanol extract of  the stem bark was investigated for in vivo antiophidian assay against Bitis arietans and Naja nigricollis. Also, the extract and chromatographic fractions were subjected to cytotoxicity tests using tadpole model and  antiarthritic assay by proteinase inhibition. Chromatography of crude methanol extract afforded three highly polar vacuum liquid chromatography (VLC) fractions (BVLC-1, BVLC-2 and BVLC-3). BVLC-2 further gave four semi-pure uncharacterized isolates (I, II, III, IV). Only BVLC-2 and BVLC-3 were cytotoxic at 10 - 80 mg/ml, with BVLC-3 being most potent (100% mortality, LC50 40 mg/ml). Concentration-dependent proteinase enzyme inhibition (24 - 71%) at 200 - 1000 μg/ml of BVLC-2 was observed, and this was less (IC50 659.24 μg/ml) than the activity of the standard drug, acetyl salicylic acid. However, neutralization of B. arietans and N. nigricollis snake venoms using methanol extract was not dose-dependent, but the extract atthe least dose, 50 mg/kg offered better protection (75%) on Naja nigricollis envenomed-mice in 48 h. Its activity was comparable to that of the positive antivenin tested at 0.2 mg/kg. These findings justify the folkloric use of T. grandis in the treatment of snake bites, arthritic conditions, and oxidative stress-induced diseases. Keywords: Tectona grandis, methanol extract, anti-snake venom activity, tadpole cytotoxicity, anti-arthritic activity

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