Wound healing potential of methanol extract of Spathodea campanulata stem bark forumlated into a topical preparation

Ficus platyphylla (Fp) has been used in Nigerian traditional medicine for the management of pain, ulcer, wound, epilepsy and inflammation. The efficacy of its gum from the stem bark for the management of wound has been widely acclaimed among the Hausa communities of Northern Nigeria and therefore, this study was aimed at examining the antimicrobial and wound healing potential of methanol extract of F. platyphylla stem bark (MEFpSB) in order to provide scientific basis for its antimicrobial and wound healing properties. Preliminary phytochemical screening was done and antimicrobial activity using some pathogenic micro-organisms were evaluated following its wound healing effectiveness in Wistar rats using the model of superficial skin excision wound. Data were analysed using one-way analysis of variance (ANOVA) followed by Dunnet’s t-test. The results of preliminary phytochemical screeningrevealed the presence of carbohydrate, cardiac glycosides, saponins, flavonoids, triterpenes, tannins, alkaloids with anthraquinones absent. The extract showed antimicrobial activity on Staphylococcus aureus, Corynebacterium ulcerans, Escherichia coli, Klebsiella pneumonia and Salmonella typhi and significant (p< 0.05, p< 0.001) decrease in wound measurement for 11 days. This study demonstrated that MEFpSB possesses antimicrobial activity with wound healing properties that justifies the ethno-medicinal use of the plant in wound/ulcer healings. Keywords: Wound healing; Antimicrobial; Ficus platyphylla; Formulation; Topical

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Antimicrobial and Wound Healing Activity of Gardenia aqualla Stem Bark Methanol Extract

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2021/v9i130207 ◽

2021 ◽

pp. 30-38

Author(s):

Michael Anyekema ◽

Lesley Sahber Kindzeka ◽

Aristide Dzelamonyuy

Keyword(s):

Antimicrobial Activity ◽

Wound Healing ◽

Methanol Extract ◽

Stem Bark ◽

Salmonella Typhi ◽

Scientific Basis ◽

Klebsiella Pneumonia ◽

Phytochemical Screening ◽

Corynebacterium Ulcerans ◽

Superficial Skin

Aim: This study was aimed at examining the antimicrobial and wound healing activities of Gardenia aqualla stem bark methanol extract (GASBME) in order to provide scientific basis for its antimicrobial and wound healing properties. Methodology: Preliminary phytochemical screening was done and antimicrobial activity determined using some pathogenic micro-organisms were evaluated following its wound healing effectiveness in Wistar rats using the model of superficial skin excision wound. Data were analysed using one-way analysis of variance (ANOVA) followed by Dunnet’s t-test. Results: The results of preliminary phytochemical screening revealed the presence of carbohydrate, cardiac glycosides, saponins, flavonoids, triterpenes, tannins, alkaloids with anthraquinones being absent. The extract showed antimicrobial activity on Staphylococcus aureus, Corynebacterium ulcerans, Escherichia coli, Klebsiella pneumonia and Salmonella typhi and significant (p< 0.05, p< 0.001) decrease in wound measurement by the 11th day. Conclusion: This study demonstrated that GASBME possesses antimicrobial activity with wound healing properties that justifies the ethno-medicinal use of the plant in wound/ulcer healings.

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Antidiabetic and biochemical effect of the different fractions of methanol extract of Detarium microcarpum (Fabaceae) Guill and Perr stem bark on normal and alloxan induced diabetic rats

Planta Medica ◽

10.1055/s-0033-1352414 ◽

2013 ◽

Vol 79 (13) ◽

Cited By ~ 1

Author(s):

UE Odoh ◽

CO Ezugwu ◽

CS Ezea

Keyword(s):

Methanol Extract ◽

Stem Bark ◽

Diabetic Rats ◽

Biochemical Effect ◽

Detarium Microcarpum

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Evaluation of reversible antiandrogenic and antispermatogenic activities of Annona squamosa (Linn) stem bark methanol extract in male albino rats

Planta Medica ◽

10.1055/s-2006-949734 ◽

2006 ◽

Vol 72 (11) ◽

Author(s):

RS Gupta ◽

A Sharma ◽

H Rehwani

Keyword(s):

Methanol Extract ◽

Stem Bark ◽

Albino Rats ◽

Annona Squamosa

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Methanol extract from the seeds of Persea americana displays antibacterial and wound healing activities in rat model

Journal of Ethnopharmacology ◽

10.1016/j.jep.2021.114573 ◽

2021 ◽

pp. 114573

Author(s):

Steve E. Ekom ◽

Jean D.D. Tamokou ◽

Victor Kuete

Keyword(s):

Wound Healing ◽

Rat Model ◽

Methanol Extract ◽

Persea Americana

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Evaluation of wound healing and antimicrobial properties of aqueous extract from Bowdichia virgilioides stem barks in mice

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652013005000049 ◽

2013 ◽

Vol 85 (3) ◽

pp. 945-954 ◽

Cited By ~ 15

Author(s):

ISABELA K.R. AGRA ◽

LUANA L.S. PIRES ◽

PAULO S.M. CARVALHO ◽

EURIPEDES A. SILVA-FILHO ◽

SALETE SMANIOTTO ◽

...

Keyword(s):

Wound Healing ◽

Latin American ◽

Aqueous Extract ◽

Antimicrobial Properties ◽

Inflammatory Cells ◽

Stem Bark ◽

Wound Contraction ◽

Type I ◽

Folk Remedy

The decoction of the stem barks from Bowdichia virgilioides KUNTH is a folk remedy used to treat inflammatory disorders in Latin American and Brazil. In the present study, the wound healing activity of aqueous extract of the stem bark from B. virgilioides, called AEBv, was evaluated by the rate of healing by wound contraction and period of epithelization at different days post-wound using the wound excisional model. On day 9, the AEBv-treated animals exhibited significative reduction in the wound area when compared with controls. In wound infected with S. aureus, the AEBv significantly improved the wound contraction when compared to the saline-treated mice. The histological analysis showed that AEBv induced a collagen deposition, increase in the fibroblast count and few inflammatory cells than compared to saline-treated group. The expression of collagen type I was increased in the group treated with AEBv as indicated by immunohistochemical staining. In vitro, the AEBv was effective only against S. aureus but not against P. aeruginosa. Together, the results of this study demonstrate, for the first time, the healing and antimicrobiological effects of aqueous extract of the stem bark from B. virgilioides in the therapy of skin wounds.

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GALLIC ACID: A PHENOLIC ACID AND ITS ANTIOXIDANT ACTIVITY FROM STEM BARK OF CHLOROFORM EXTRACTS OF SYZYGIUM LITORALE (BLUME) AMSHOFF (MYRTACEAE)

Molekul ◽

10.20884/1.jm.2016.11.2.215 ◽

2016 ◽

Vol 11 (2) ◽

pp. 180 ◽

Cited By ~ 1

Author(s):

Tukiran Tukiran ◽

Fitriyatul Mahmudah ◽

Nurul Hidayati ◽

Kuniyoshi Shimizu

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Phenolic Acid ◽

Methanol Extract ◽

Authentic Sample ◽

Stem Bark ◽

Spectroscopic Studies ◽

Chloroform Fraction ◽

Ic50 Value ◽

Isolated Compound

A phenolic acid had been isolated from chloroform soluble fractions of a methanol extract of stem bark of Syzygium litorale, Fam. Myrtaceae. The structure of the isolated compound was elucidated and established as gallic acid through extensive spectroscopic studies (UV-Vis, FTIR, and NMR) and by comparison with literature data and authentic sample. This is the first report of the isolation of compound from this plant, although it has previously been found in Myrtaceae family such as S. aromaticum, S. cumini, S. polyanthum, S. cordatum, etc. The chloroform fraction, isolated compound, and vitamin C showed very strong antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) with IC50 value of 23.2, 7.5, and 12.5 mg/mL, respectively.

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Pharmacological potential of methanol extract of Anacardium occidentale stem bark on alloxan-induced diabetic rats

Biomedical Research and Therapy ◽

10.15419/bmrat.v5i7.456 ◽

2018 ◽

Vol 5 (7) ◽

pp. 2440-2454

Author(s):

D. A. Omoboyowa ◽

F. O. Afolabi ◽

T. C. Aribigbola

Keyword(s):

Blood Glucose ◽

Glucose Tolerance ◽

Glucose Tolerance Test ◽

Methanol Extract ◽

Stem Bark ◽

Tolerance Test ◽

Diabetic Rats ◽

Oral Glucose ◽

Oral Glucose Tolerance ◽

Β Cells

Background: The anti-hyperglycemic potential of methanol stem bark extract of Anacardium occidentale (MSBEAO) was investigated using an alloxan-induced diabetic rat model. Alloxan administration induces the generation of free radicals which can affect antioxidant status resulting in the disruption of the β-cells of the pancreas. Therefore, this study examines the antioxidant potential of the plant extract and the ameliorating effect on the pancreas of alloxan-induced diabetic rats. Methods: Diabetes was induced by intraperitoneal injection of 150 mg/kg body weight of alloxan monohydrate. MSBEAO, at a concentration of 100 or 200 mg/kg b.w. was orally administered to alloxan-induced diabetic rats and normal rats. The hypoglycemic effect, oral glucose tolerance test, and biochemical assay of alloxan-induced diabetic rats were assayed using standard procedures. Results: Preliminary phytochemical screening of the extract revealed the presence of alkaloids, tannins, saponins, terpenoids, carbohydrates, and phenols at moderate concentrations. The lethality dose (LD50) of the plant extract was found to be equal to or less than 5000 mg/kg b.w. The hypoglycemic effect of the extract on the non-diabetic rats revealed a significant (p<0.05) decrease in the blood glucose concentration of animals administered with 1 g/kg b.w. of the extract, compared to normal control rats administered with normal saline. In the oral glucose tolerance test, the methanol extract exerted the highest response, similar to glibenclamide after 15 and 30 minutes of administration, compared to the control rats. The methanol extract yielded the highest blood glucose lowering effects after 9 days of treatment (p<0.05), compared to diabetic rats administered with normal saline and 0.3 mg/kg b.w. of glibenclamide. Administration of the extract at 200 mg/kg b.w. showed improved pancreas architecture and regeneration of the β-cells, compared with the pancreas of animals in the other groups. Conclusion: The results of this study suggest that MSBEAO is a potentially effective agent for the management of diabetes which might result from the antioxidant-generating capacity of the stem bark.

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THE PHYTOCHEMICAL SCREENING HEXANE AND METHANOL EXTRACT OF SINYO NAKAL (DURANTA REPENS)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i8.38165 ◽

2020 ◽

pp. 196-200

Author(s):

PURWANTININGSIH SUGITA ◽

RIZKI AMILIA ◽

BUDI ARIFIN ◽

DYAH UTAMI CAHYANING RAHAYU ◽

HANHAN DIANHAR

Keyword(s):

Fatty Acids ◽

Developing Countries ◽

Linoleic Acid ◽

Vitamin E ◽

Methanol Extract ◽

Stem Bark ◽

Unsaponifiable Fraction ◽

Phytochemical Screening ◽

Phytochemical Profile ◽

Based Medicine

Objective: Phytochemicals are naturally present in every part of plants range from leaves, stem bark, and fruits to roots. The use of plant-based medicine is popular among individuals and communities in developing countries. This study evaluated that the phytochemical profile of Duranta repens fruits was collected from Jombang, East Java, Indonesia. Methods: Air-dried D. repens fruits were macerated by n-hexane and methanol, a separate flask. n-Hexane extract, then underwent saponification to remove fat. Then, the unsaponifiable of n-hexane and methanol extract was tested through phytochemical screening, respectively. Results: The methanol extract showed the presence of alkaloids, flavonoids, tannins, and steroids, but the unsaponifiable fraction only contained steroids. The unsaponifiable fraction was found to be a mixture of hydrocarbons ranging from C13-C20, fatty acids (palmitic and linoleic acid), squalene, and Vitamin E. Conclusion: The phytochemical screening of the plant plays an important role for pharmaceutical studies especially discovering new potential drugs for the treatment of various diseases.

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