In vitro propagation of Nutmeg, Myristica Fragrans Houtt

2007 ◽  
pp. 335-344
Author(s):  
R. I. Iyer
Keyword(s):  
In Vitro Propagation ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

scholarly journals EKSPLORASI DAN IDENTIFIKASI CENDAWAN ANTAGONIS TERHADAP JAMUR AKAR PUTIH (Rigidoporus lignosus) PADA TANAMAN PALA (Myristica fragrans Houtt)

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Nana Ariska ◽  
Lola Adres Yanti ◽  
Chairudin Chairudin
Keyword(s):  
Economic Value ◽  
Culture Method ◽  
Dilution Method ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Pests And Diseases ◽  
Myristica Fragrans ◽  
Rigidoporus Lignosus ◽  
Myristica Fragrans Houtt

Nutmeg (Myristica fragrans Houtt) is a multipurpose plantation commodity crop that almost all parts of the plant have strategic economic value to be cultivated. In Indonesia there are the largest nutmeg commodity centers, including the Maluku islands, North Sulawesi and Aceh. The biggest problem with nutmeg plants is the attack of pests and diseases. One of the most severe diseases in nutmeg is white root mushroom (Rigidoporus lignosus). This study aims to 1) determine the fungus of white root fungus antagonists (Rigodoporus lignosus) from the Aceh nutmeg rhizosphere (Myristica fragrans Houtt), 2) identify the content of antibiotic fungus JAP antagonist compounds in Aceh nutmeg plants. The study was conducted by taking soil samples from nutmeg plants at three nutmeg plantations in South Aceh. The antagonist fungus isolation activity uses a dilution method. Isolation of JAP from infected roots using direct planting method. Furthermore, it is identified macroscopically and microscopically. The antagonist test was carried out using the dual culture method with RAL. From the rhizosphere in the nutmeg area, 5 candidate antagonist isolates were obtained, having high in vitro antagonism ability to the pathogen R. lignosus. Identification results showed that isolates 1, 2 and 3 were Trichoderma spp, and isolates 4 and 5 were Gliocladium virens. Keywords: In vitro, the content of antibiotic compounds, Aceh nutmeg, Rigidoporus lignosus, antagonistic test

scholarly journals Pengaruh ekstrak biji pala (Myristica fragrans Houtt) sebagai antibakteri terhadap pertumbuhan Pseudomonas aeruginosa

2018 ◽  
Vol 1 (3) ◽  
pp. 172-178
Author(s):  
Fiadwita Nia Ifriana ◽  
Widyasari Kumala
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

LATAR BELAKANGPseudomonas aeruginosa merupakan bakteri negatif-gram yang menjadi penyebab tersering infeksi di lingkungan Rumah Sakit yang dikenal dengan istilah infeksi nosokomial. Saat ini penggunaan antibakteri dari bahan alami mulai dikembangkan. Di seluruh dunia, ratusan tanaman dapat digunakan dalam pengobatan tradisional sebagai pengobatan untuk infeksi bakteri. Biji buah pala (Myristica fragrans Houtt) mengandung minyak atsiri, saponin, terpenoid, flavonoid, yang diketahui berpotensi sebagai antibakteri. Maka dari itu penulis ingin mengetahui aktivitas antibakteri pada biji pala (Myristica fragrans Houtt) terhadap pertumbuhan bakteri Pseudomonas aeruginosa. METODEDesain penelitian ini adalah eksperimental sesungguhnya (True Experimental) laboratorium in vitro, menggunakan metode difusi cakram, dengan menggunakan sampel ekstrak biji pala (Myristica fragrans Houtt) dan bakteri Pseudomonas aeruginosa. Terdapat 4 kelompok perlakuan dan 2 kelompok kontrol. Zona hambat yang terbentuk akan diukur menggunakan penggaris. Analisis data dengan menggunakan SPSS versi 22. HASILHasil penelitian menunjukkan ekstrak biji pala (Myristica fragrans Houtt) dapat menghambat pertumbuhan bakteri Pseudomonas aeruginosa, hal ini dibuktikan dengan terbentuknya zona hambat pada kertas cakram. Zona hambat yang terbentuk bervariasi, semakin tinggi konsentrasi ekstrak maka semakin luas zona hambat yang terbentuk. KESIMPULANEkstrak biji pala (Myristica fragrans Houtt) dapat menghambat pertumbuhan Pseudomonas aeruginosa dengan kategori lemah.

scholarly journals Antimutagenic effects of aqueous fraction of Myristica fragrans (Houtt.) leaves on Salmonella typhimurium and Mus musculus.

2014 ◽  
Vol 61 (4) ◽  
Author(s):  
Akeem Akinboro ◽  
Kamaruzaman Bin Mohamed ◽  
Mohd Zaini Asmawi ◽  
Taofeek A Yekeen
Keyword(s):  
Salmonella Typhimurium ◽  
Mus Musculus ◽  
Ultra Performance Liquid Chromatography ◽  
Total Phenolic ◽  
Aqueous Fraction ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt ◽  
Phenolic And Flavonoid

Natural plant extracts offer a promising hope in the prevention/treatment of cancer arising from genetic mutations. This study evaluated in vitro and in vivo mutagenic and antimutagenic effects of aqueous fraction of Myristica fragrans (AFMF) leaves on TA100 strain of Salmonella typhimurium and Mus musculus (Male Swiss albino mice), respectively. The antioxidant activity of AFMF against 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic and flavonoid contents were determined, followed by its phytochemical elucidation using the Ultra Performance Liquid Chromatography technique (UPLC). The mutagenicity of AFMF at 4, 20, 50, 100, 200, 500, and 1000 µg/well was

scholarly journals Enzyme Inhibition by Molluscicidal Components of Myristica fragrans Houtt. in the Nervous Tissue of Snail Lymnaea acuminata

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Preetee Jaiswal ◽  
Pradeep Kumar ◽  
V. K. Singh ◽  
D. K. Singh
Keyword(s):  
Nervous Tissue ◽  
Freshwater Snail ◽  
Inhibition Kinetics ◽  
Acid And Alkaline Phosphatase ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt ◽  
Kinetics Of ◽  
Lymnaea Acuminata

This study was designed to investigate the effects of molluscicidal components of Myristica fragrans Houtt. (Myristicaceae) on certain enzymes in the nervous tissue of freshwater snail Lymnaea acuminata Lamarck (Lymnaeidae). In vivo and in vitro treatments of trimyristin and myristicin (active molluscicidal components of Myristica fragrans Houtt.) significantly inhibited the acetylcholinesterase (AChE), acid and alkaline phosphatase (ACP/ALP) activities in the nervous tissue of Lymnaea acuminata. The inhibition kinetics of these enzymes indicates that both the trimyristin and myristicin caused competitive noncompetitive inhibition of AChE. Trimyristin caused uncompetitive and competitive/noncompetitive inhibitions of ACP and ALP, respectively whereas the myristicin caused competitive and uncompetitive inhibition of ACP and ALP, respectively. Thus results from the present study suggest that inhibition of AChE, ACP, and ALP by trimyristin and myristicin in the snail Lymnaea acuminata may be the cause of the molluscicidal activity of Myristica fragrans.

scholarly journals Uji Aktivitas Minyak Atsiri Daun Pala (Myristica fragrans Houtt.) terhadap Malassezia furfur secara In Vitro

2021 ◽  
Vol 5 (4B) ◽  
pp. 1549
Author(s):  
Qurratul Aini ◽  
Muhamad Agus Wibowo ◽  
Mahyarudin Mahyarudin
Keyword(s):  
Pityriasis Versicolor ◽  
Tween 20 ◽  
Malassezia Furfur ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt ◽  
Post Hoc ◽  
One Way Anova

Latar Belakang. Pityriasis versicolor atau panu merupakan infeksi jamur superfisial pada kulit yangdisebabkan oleh Malassezia furfur. Minyak atsiri daun pala diketahui mengandung senyawa metabolit sekunder yang berpotensi sebagai antijamur. Metode. Penelitian ini merupakan eksperimental murni menggunakan Rancangan Acak Lengkap (RAL). Uji aktivitas antijamur menggunakan metode difusi cakram Kirby-Bauer dengan variasi konsentrasi 10%, 25%, 50%, 75% dan 100%. Kontrol positif yang digunakan adalah ketokonazol 30 µg/disk sedangkan kontrol negatif yang digunakan adalah Tween 20. Analisis data menggunakan uji statistic One Way ANOVA dan dilanjutkan dengan uji Post Hoc Least Significant Different (LSD). Hasil. Hasil uji aktivitas antijamur minyak atsiri daun pala pada konsentrasi 25%, 50%, 75% dan 100% membentuk zona hambat dengan rata-rata diameter sebesar 8,875mm, 9,625mm, 10mm, dan 15mm. Uji statistik menunjukkan nilai signifikansi (p < 0,05) yang mengindikasikan terdapat perbedaan bermakna antar kelompok perlakuan. Kesimpulan. Minyak atsiri daun pala memiliki aktivitas antijamur terhadap Malassezia furfur.

scholarly journals Uji daya hambat ekstrak buah pala (myristica fragrans Houtt) terhadap bakteri penyebab periodontitis porphyromonas gingivalis secara in vitro

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Pricillia T. Kaawoan ◽  
Jemmy Abidjulu ◽  
Krista V. Siagian
Keyword(s):  
Porphyromonas Gingivalis ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Myristica Fragrans ◽  
Post Test ◽  
Myristica Fragrans Houtt

Abstract: Periodontal disease is preceded by a buildup of plaque that contains a collection of bacteria. The most common bacteria found in plaques are Porphyromonas gingivalis that cause periodontitis. There are several ways to treat periodontitis inter alia the usage of natural materials. Nutmeg (Myristica fragrans Houtt) contains volatile oil, saponins, and alkaloids known as antibacterials. This study aimed to investigate the inhibitory effect of nutmeg extract on Porphyromonas gingivalis. This was an experimental laboratory study in vitro with a post test only control group design. The testing method used in this study was a modified method of Kirby-bauer. Nutmeg was extracted by using maceration method with ethanol 96%. Porphyromonas gingivalis bacteria were ordered from University of Hasanuddin Makassar and then were rejuvenated in the Laboratory of Microbiology Pharmacy FMIPA University of Sam Ratulangi Manado. The results showed that the average inhibitory zone of nutmeg extract was 13,5 mm. Conclusion: Nutmeg (Myristica fragrans Houtt) extract had an inhibitory effect on the Porphyromonas gingivalis bacteria.Keywords: nutmeg (Myristica fragrans Houtt), inhibition zone, periodontitis, Porphyromonas gingivalisAbstrak: Penyakit periodontal berawal dari penumpukan plak yang mengandung kumpulan bakteri. Bakteri yang paling banyak ditemukan yaitu bakteri Porphyromonas gingivalis yang menyebabkan penyakit periodontitis. Terdapat beberapa cara untuk mengobati periodontitis, salah satunya dengan penggunaan bahan alami. Pala (Myristica fragrans Houtt) memiliki kandungan minyak atsiri, saponin, dan alkaloida yang diketahui berefek antibakteri. Penelitian ini bertujuan untuk mengetahui adanya daya hambat ekstrak buah pala terhadap bakteri penyebab periodontitis Porphyromonas gingivalis. Jenis penelitian ini ialah eksperimental laboratorik secara in vitro dengan post test only control group design. Metode pengujian yang digunakan yaitu modifikasi Kirby-bauer menggunakan sumuran. Sampel buah pala diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Bakteri Porphyromonas gingivalis yang digunakan dalam penelitian ini dikirim dari Universitas Hasanuddin Makassar yang telah diremajakan di Laboratorium Mikrobiologi Farmasi FMIPA Universitas Sam Ratulangi Manado. Hasil penelitian mendapatkan zona hambat ekstrak buah pala sebesar 13,5 mm. Simpulan: Ekstrak buah pala (Myristica fragrans Houtt) mempunyai daya hambat terhadap bakteri penyebab periodontitis Porphyromonas gingivalis.Kata kunci: pala (Myristica fragrans Houtt), zona hambat, periodontitis, porphyromonas gingivalis

Screening for PPAR γ agonist from Myristica fragrans Houtt seeds for the treatment of Type 2 diabetes by in vitro and in vivo

2012 ◽  
Vol 12 (3) ◽  
pp. 7-15 ◽  
Author(s):  
Keri Lestari ◽  
JK Hwang ◽  
Sri Hartini Kariadi ◽  
Andi Wijaya ◽  
Trihanggono Ahmad ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Ppar Γ ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

scholarly journals EVALUATION OF ANTI-DIABETIC ACTIVTY OF DIFFERENT EXTRACTS OF MYRISTICA FRAGRANS HOUTT: IN VITRO AND IN SILICO STUDIES

2017 ◽  
Vol 10 (4) ◽  
pp. 275
Author(s):  
Saranya Sivaraj ◽  
Gomathi Kannayiram ◽  
Gayathri Dasararaju
Keyword(s):  
Molecular Docking ◽  
In Silico ◽  
Inhibitory Effect ◽  
Docking Studies ◽  
Alpha Amylase ◽  
Myristica Fragrans ◽  
In Silico Studies ◽  
Myristica Fragrans Houtt ◽  
In Silico Molecular Docking

Objective: This study is aimed to evaluate the anti-diabetic effect of sequentially extracted (hexane, dichloromethane, ethyl acetate, and ethanol) Myristica fragrans houtt (mace) through in vitro and in silico studies. Methods: The in vitro anti-diabetic effect of the sequentially extracted plant were evaluated for its alpha-amylase inhibitory activity and the potential binding was studied by in silico studies using Schrödinger Maestro.Results: All extracts showed dose dependent alpha-amylase inhibitory effect. At concentration 500 µg/ml, all the extracts showed more than 60% inhibition of the alpha-amylase enzyme and the highest inhibition (81.30%) at 500 µg/ml was observed in DCM extract of mace. Potential compounds were identified by in silico molecular docking studies of alpha-amylase with phytocomponents from DCM extract. Among the top three compounds from virtual screening, induced fit docking studies revealed 2,5-bis(3,4-dimethoxyphenyl)-3,4-dimethyloxolane possessed better binding affinity when compared with the drug metformin. Conclusion: The obtained in vitro and in silico results suggest that all extracts of Myristica fragrans can be used successfully for the management of diabetes mellitus.Keywords: Myristica fragrans, Mace, Sequential extraction, Alpha-amylase, Molecular docking.

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