A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana

2013 ◽  
pp. 119-123 ◽  
Author(s):  
Susan Armstrong
Keyword(s):  
Arabidopsis Thaliana ◽  
Time Course ◽  
Meiotic Progression

scholarly journals The Geomagnetic Field (GMF) Modulates Nutrient Status and Lipid Metabolism during Arabidopsis thaliana Plant Development

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1729
Author(s):  
Monirul Islam ◽  
Gianpiero Vigani ◽  
Massimo E. Maffei
Keyword(s):  
Magnetic Field ◽  
Arabidopsis Thaliana ◽  
Lipid Metabolism ◽  
Plant Development ◽  
Stress Responses ◽  
Geomagnetic Field ◽  
Time Course ◽  
Seed Set ◽  
Developmental Stages ◽  
Lipid Transfer Protein

The Geomagnetic field (GMF) is a typical component of our planet. Plant perception of the GMF implies that any magnetic field (MF) variation would induce possible metabolic changes. In this work was we assessed the role of the GMF on Arabidopsis thaliana Col0 mineral nutrition and lipid metabolism during plant development. We reduced the local GMF (about 40 μT) to Near Null Magnetic Field (NNMF, about 30 nT) to evaluate the effects of GMF on Arabidopsis in a time-course (from rosette to seed-set) experiment by studying the lipid content (fatty acids, FA; and surface alkanes, SA) and mineral nutrients. The expression of selected genes involved in lipid metabolism was assessed by Real-Time PCR (qPCR). A progressive increase of SA with carbon numbers between 21 and 28 was found in plants exposed to NNMF from bolting to flowering developmental stages, whereas the content of some FA significantly (p < 0.05) increased in rosette, bolting and seed-set developmental stages. Variations in SA composition were correlated to the differential expression of several Arabidopsis 3-ketoacyl-CoAsynthase (KCS) genes, including KCS1, KCS5, KCS6, KCS8, and KCS12, a lipid transfer protein (LTPG1) and a lipase (LIP1). Ionomic analysis showed a significant variation in some micronutrients (Fe, Co, Mn and Ni) and macronutrients (Mg, K and Ca) during plant development of plants exposed to NNMF. The results of this work show that A. thaliana responds to variations of the GMF which are perceived as is typical of abiotic stress responses.

A model-based analysis of the dynamics of carbon balance at the whole-plant level in Arabidopsis thaliana

2008 ◽  
Vol 35 (11) ◽  
pp. 1147 ◽  
Author(s):  
Angélique Christophe ◽  
Véronique Letort ◽  
Irène Hummel ◽  
Paul-Henry Cournède ◽  
Philippe de Reffye ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Growth Analysis ◽  
Time Course ◽  
Strong Increase ◽  
Structural Development ◽  
Whole Plant ◽  
Use Efficiency ◽  
Plant Level ◽  
Source Sink ◽  
Radiation Use

Arabidopsis thaliana (L.) Heynh. is used as a model plant in many research projects. However, few models simulate its growth at the whole-plant scale. The present study describes the first model of Arabidopsis growth integrating organogenesis, morphogenesis and carbon-partitioning processes for aerial and subterranean parts of the plant throughout its development. The objective was to analyse competition among sinks as they emerge from patterns of plant structural development. The model was adapted from the GreenLab model and was used to estimate organ sink strengths by optimisation against biomass measurements. Dry biomass production was calculated by a radiation use efficiency-based approach. Organogenesis processes were parameterised based on experimental data. The potential of this model for growth analysis was assessed using the Columbia ecotype, which was grown in standard environmental conditions. Three phases were observed in the overall time course of trophic competition within the plant. In the vegetative phase, no competition was observed. In the reproductive phase, competition increased with a strong increase when lateral inflorescences developed. Roots and internodes and structures bearing siliques were strong sinks and had a similar impact on competition. The application of the GreenLab model to the growth analysis of A. thaliana provides new insights into source–sink relationships as functions of phenology and morphogenesis.

Time course of meiotic progression after transferring primary spermatocyte into ooplasm at different stages

2007 ◽  
Vol 74 (9) ◽  
pp. 1072-1080 ◽  
Author(s):  
Chang-Long Nan ◽  
Ying-Chun OuYang ◽  
Zhen-Jun Zhao ◽  
Yan Jiang ◽  
Zi-Li Lei ◽  
...  
Keyword(s):  
Time Course ◽  
Primary Spermatocyte ◽  
Meiotic Progression

scholarly journals Cloning cDNAs for Genes Preferentially Expressed during Fruit Growth in Cucumber

1999 ◽  
Vol 124 (2) ◽  
pp. 136-139 ◽  
Author(s):  
Takuro Suyama ◽  
Kunio Yamada ◽  
Hitoshi Mori ◽  
Kiyotoshi Takeno ◽  
Shohei Yamaki
Keyword(s):  
Arabidopsis Thaliana ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Ribosomal Protein ◽  
Time Course ◽  
Large Subunit ◽  
Fruit Growth ◽  
Cdna Clones ◽  
Subtraction Hybridization ◽  
Ribosomal Protein L3

A cDNA library was constructed from poly(A)+RNA extracted from pollinated fruit of `PMR-142' cucumber (Cucumis sativus L.). Subtraction hybridization was made between the cDNAs and poly(A)+RNA from unpollinated fruit to isolate cDNA clones that corresponded to the genes preferentially expressed in the pollinated fruit. We isolated three cDNAs, which were 756, 826, and 998 nucleotides long and designated Csf1, Csf2, and Csf3, respectively. When fruit growth was triggered by pollination, auxin treatment and natural parthenocarpy, Csf2 was always expressed. Time course of expression of the Csf2 gene was nearly parallel to that of the fruit growth. Nucleotide sequences of the Csf cDNAs were fully determined. Homology of the deduced amino acid sequence for Csf1 showed 75% identity with a pea extensin. Only 37%, 33%, and 26% homology was found between Csf2 and bell pepper CaSn-2, tobacco FB7-4, and opium poppy gMLP15, respectively. The Csf3 sequence showed 68% identity with the large subunit of 60S ribosomal protein L3 of Arabidopsis thaliana.

scholarly journals Autophagy is responsible for the accumulation of proteogenic dipeptides in response to heat stress in Arabidopsis thaliana

2020 ◽  
Author(s):  
Venkatesh P. Thirumalaikumar ◽  
Mateusz Wagner ◽  
Salma Balazadeh ◽  
Aleksandra Skirycz
Keyword(s):  
Heat Treatment ◽  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
Small Molecule ◽  
Time Course ◽  
High Density ◽  
Loss Of Function ◽  
Proteolytic Pathway ◽  
Stress Experiment

AbstractProteogenic dipeptides are intermediates of proteolysis as well as an emerging class of small-molecule regulators with diverse and often dipeptide-specific functions. Herein, prompted by differential accumulation of dipeptides in a high-density Arabidopsis thaliana time-course stress experiment, we decided to pursue an identity of the proteolytic pathway responsible for the buildup of dipeptides under heat conditions. By querying dipeptide accumulation versus available transcript data, autophagy emerged as a top hit. To examine whether autophagy indeed contributes to the accumulation of dipeptides measured in response to heat stress, we characterized the loss-of-function mutants of crucial autophagy proteins to test whether interfering with autophagy would affect dipeptide accumulation in response to the heat treatment. This was indeed the case. This work implicates the involvement of autophagy in the accumulation of proteogenic dipeptides in response to heat stress in Arabidopsis.

scholarly journals Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment*

2006 ◽  
Vol 58 (3) ◽  
pp. 415-424 ◽  
Author(s):  
Jae Kwang Kim ◽  
Takeshi Bamba ◽  
Kazuo Harada ◽  
Eiichiro Fukusaki ◽  
Akio Kobayashi
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Cell Cultures ◽  
Metabolic Profiling ◽  
Time Course ◽  
Stress Treatment

scholarly journals Heat stress reveals the existence of a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana

2021 ◽  
Author(s):  
Joke de Jager-Braet ◽  
Linda Krause ◽  
Anika Buchholz ◽  
Arp Schnittger
Keyword(s):  
Arabidopsis Thaliana ◽  
Meiotic Recombination ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Early Prophase ◽  
Sudden Increase ◽  
Atm Kinase ◽  
Meiotic Progression ◽  
Temperature Regimes ◽  
Recombination Pathway

Plant growth and fertility strongly depend on environmental conditions such as temperature. Remarkably, temperature also influences meiotic recombination and thus, the current climate change will affect the genetic make-up of plants. To further understand temperature effects on meiosis, we have followed male meiocytes of Arabidopsis thaliana by live cell imaging under three different temperature regimes, at 21C and at heat shock conditions of 30C and 34C as well as after an acclimatization phase of one week at 30C. This work led to a cytological framework of meiotic progression at elevated temperature. We found that an increase to 30C, sped up meiotic progression with specific phases being more amenable to heat than others. An acclimatization phase often moderated this effect. A sudden increase to 34C promoted a faster progression of meiosis in early prophase compared to 21C. However, the phase in which cross-overs maturate was found to be delayed at 34C. Interestingly, mutants involved in the recombination pathway did not show the extension of this phase at 34C demonstrating that the delay is recombination dependent. Further analysis revealed the involvement of the ATM kinase in this prolongation indicating the existence of a specialized variant of the pachytene checkpoint in plants.

scholarly journals Widespread inter-individual gene expression variability in Arabidopsis thaliana

2018 ◽  
Author(s):  
Sandra Cortijo ◽  
Zeynep Aydin ◽  
Sebastian Ahnert ◽  
James Locke
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Time Course ◽  
Gene Expression Regulation ◽  
Individual Variability ◽  
Individual Gene ◽  
Expression Variability ◽  
Unicellular Organisms ◽  
Environmental Responses ◽  
The Impact

AbstractA fundamental question in biology is how gene expression is regulated to give rise to a phenotype. However, transcriptional variability is rarely considered and could influence the relationship between genotype and phenotype. It is known in unicellular organisms that gene expression is often noisy rather than uniform and this has been proposed to be beneficial when environmental conditions are unpredictable. However, little is known about transcriptional variability in plants. Using transcriptomic approaches, we analysed gene expression variability between individual Arabidopsis thaliana plants growing in identical conditions over a 24 hour time-course. We identified hundreds of genes that exhibit high inter-individual variability and found that many are involved in environmental responses. We also identified factors that might facilitate gene expression variability, such as gene length, the number of transcription factors regulating the genes and the chromatin environment. These results shed new light on the impact of transcriptional variability in gene expression regulation in plants.

A meiotic time-course for Arabidopsis thaliana

2003 ◽  
Vol 16 (3) ◽  
pp. 141-149 ◽  
Author(s):  
S. J. Armstrong ◽  
F. C. H. Franklin ◽  
G. H. Jones
Keyword(s):  
Arabidopsis Thaliana ◽  
Time Course
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