Mechanisms of (−)-Epigallocatechin Gallate and Green Tea in Inhibition of Carcinogenesis

The aim of this study was to investigate epigallocatechin gallate (EGCG) prebiotics activities systematically which was reported as a bioactive substance. Therefore, EGCG was separated by water extraction, resin purification and prep-HPLC. Then the production of EGCG was confirmed by HPLC and mass spectrometry (MS) analysis and its purify was 97.23%. EGCG extractive and green tea extract (GTE) were further incubated with Bifidobacterium infantis, B. adolescentis, B. bifidum and Lactobacillus acidophilus to study its effect on microbial populations and medium pH. Finally, Escherichia coli, Salmonella, Staphylococcus aureus and Candida albicans were employed as pathogenic bacteria to explore the antimicrobial activity of EGCG and GTE. The results demonstrated that EGCG extractive could be beneficial for the proliferation of Bifidobacterium and L. acidophilus and also inhibit some pathogenic bacteria. In conclusion, both EGCG extractive and GTE had prebiotics activities and the effects of EGCG extractive were superior to those of GTE.

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Effect of Green Tea and (-)-Epigallocatechin Gallate on the Pharmacokinetics of Rosuvastatin

Current Drug Metabolism ◽

10.2174/1389200221666200514133355 ◽

2020 ◽

Vol 21 (6) ◽

pp. 471-478

Author(s):

Shenjia Huang ◽

Qingqing Xu ◽

Linsheng Liu ◽

Yicong Bian ◽

Shichao Zhang ◽

...

Keyword(s):

Green Tea ◽

Cell Model ◽

Hek293t Cell ◽

Epigallocatechin Gallate ◽

Drug Uptake ◽

Pharmacokinetic Parameters ◽

Control Group ◽

Sprague Dawley ◽

Time Curve ◽

Uptake And Transport

Background: Green tea can inhibit OATPs, so it may interact with the substrate of OATPs, such as rosuvastatin. Objective: This study aimed to investigate the effects of green tea on the pharmacokinetics of rosuvastatin and its mechanism. Methods: Male Sprague-Dawley rats received different doses of green tea extract (GTE) and (-)- epigallocatechin-3- gallate (EGCG). Caco-2 cells and OATP1B1-HEK293T cells were used in drug uptake and transport assay. The matrix concentrations of rosuvastatin and catechins were determined by ultra-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS). Results: GTE and EGCG were both found to increase the area under the plasma concentration-time curve (AUC0-∞) of rosuvastatin ((p<0.050). In the Caco-2 cell model, the uptake and transport of rosuvastatin in the GTE groups were 1.94-fold (p<0.001) and 2.11-fold (p<0.050) higher, respectively, than those of the control group. However, in the EGCG group, the uptake and transport of rosuvastatin were decreased by 22.62% and 44.19%, respectively (p<0.050). In the OATP1B1- HEK293T cell model, the OATP1B1-mediated rosuvastatin uptake was decreased by GTE to 35.02% of that in the control (p<0.050) and was decreased by EGCG to 45.61% of that in the control (p<0.050). Conclusion: GTE increased the systemic rosuvastatin exposure in rats. The mechanism may include an increase in rosuvastatin absorption and a decrease in liver distribution by inhibiting OATP1B1. EGCG may be the main ingredient of green tea that affects the pharmacokinetic parameters of rosuvastatin. Our results showed the importance of conducting green tea-rosuvastatin study.

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Versatile Health Benefits of Catechin from Green Tea (Camellia sinensis)

Current Nutrition & Food Science ◽

10.2174/1573401313666171003150503 ◽

2019 ◽

Vol 15 (1) ◽

pp. 3-10 ◽

Cited By ~ 4

Author(s):

Satheesh Babu Natarajan ◽

Suriyakala Perumal Chandran ◽

Sahar Husain Khan ◽

Packiyaraj Natarajan ◽

Karthiyaraj Rengarajan

Keyword(s):

Green Tea ◽

Camellia Sinensis ◽

Health Benefits ◽

Epigallocatechin Gallate ◽

Extraction Methods ◽

Major Constituent ◽

Epicatechin Gallate ◽

Wide Range ◽

Green Tea Catechin ◽

Anti Inflammatory

Background: Tea (Camellia sinensis, Theaceae) is the second most consumed beverage in the world. Green tea is the least processed and thus contain rich antioxidant level, and believed to have most of the health benefits. Methods: We commenced to search bibliographic collection of peer reviewed research articles and review articles to meet the objective of this study. Results: From this study, we found that the tea beverage contains catechins are believed to have a wide range of health benefits which includes neuroprotective, anti-inflammatory, antiulcer, antiviral, antibacterial, and anti-parasitic effects. The four major catechin compounds of green tea are epigallocatechin (EGC), epicatechin (EC), epigallocatechin gallate (EGCG), and epicatechin gallate (ECG), of which EGCG is the major constituent and representing 50-80% of the total catechin content. And also contain xanthine derivatives such as caffeine, theophylline, and theobromine, and the glutamide derivative theanine. It also contains many nutritional components, such as vitamin E, vitamin C, fluoride, and potassium. We sum up the various green tea phytoconstituents, extraction methods, and its medicinal applications. Conclusion: In this review article, we have summarized the pharmacological importance of green tea catechin which includes antioxidant potential, anti-inflammatory, antimicrobial, anticancer, antidiabetic and cosmetic application.

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Green tea extract and its major component epigallocatechin gallate inhibits hepatitis B virus in vitro

Antiviral Research ◽

10.1016/j.antiviral.2007.11.011 ◽

2008 ◽

Vol 78 (3) ◽

pp. 242-249 ◽

Cited By ~ 54

Author(s):

Jun Xu ◽

Jue Wang ◽

Fei Deng ◽

Zhihong Hu ◽

Hualin Wang

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Green Tea ◽

Epigallocatechin Gallate ◽

Green Tea Extract ◽

B Virus ◽

Tea Extract

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Protective effect of green tea polyphenol (-)-epigallocatechin gallate and other antioxidants on lipid peroxidation in gerbil brain homogenates

Phytotherapy Research ◽

10.1002/ptr.1090 ◽

2003 ◽

Vol 17 (3) ◽

pp. 206-209 ◽

Cited By ~ 57

Author(s):

Seong-Ryong Lee ◽

Ki-Jo Im ◽

Seong-Il Suh ◽

Jung-Gil Jung

Keyword(s):

Lipid Peroxidation ◽

Protective Effect ◽

Green Tea ◽

Epigallocatechin Gallate ◽

Tea Polyphenol ◽

Gerbil Brain ◽

Green Tea Polyphenol

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Green Tea and Epigallocatechin Gallate (EGCG) for the Management of Nonalcoholic Fatty Liver Diseases (NAFLD): Insights into the Role of Oxidative Stress and Antioxidant Mechanism

Antioxidants ◽

10.3390/antiox10071076 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1076

Author(s):

Guoyi Tang ◽

Yu Xu ◽

Cheng Zhang ◽

Ning Wang ◽

Huabin Li ◽

...

Keyword(s):

Oxidative Stress ◽

Fatty Liver ◽

Green Tea ◽

Liver Diseases ◽

Randomized Clinical Trials ◽

Epigallocatechin Gallate ◽

Protective Effects ◽

Nonalcoholic Fatty Liver ◽

Nonalcoholic Fatty Liver Diseases ◽

Underlying Mechanisms

Nonalcoholic fatty liver diseases (NAFLD) represent a set of liver disorders progressing from steatosis to steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma, which induce huge burden to human health. Many pathophysiological factors are considered to influence NAFLD in a parallel pattern, involving insulin resistance, oxidative stress, lipotoxicity, mitochondrial dysfunction, endoplasmic reticulum stress, inflammatory cascades, fibrogenic reaction, etc. However, the underlying mechanisms, including those that induce NAFLD development, have not been fully understood. Specifically, oxidative stress, mainly mediated by excessive accumulation of reactive oxygen species, has participated in the multiple NAFLD-related signaling by serving as an accelerator. Ameliorating oxidative stress and maintaining redox homeostasis may be a promising approach for the management of NAFLD. Green tea is one of the most important dietary resources of natural antioxidants, above which epigallocatechin gallate (EGCG) notably contributes to its antioxidative action. Accumulative evidence from randomized clinical trials, systematic reviews, and meta-analysis has revealed the beneficial functions of green tea and EGCG in preventing and managing NAFLD, with acceptable safety in the patients. Abundant animal and cellular studies have demonstrated that green tea and EGCG may protect against NAFLD initiation and development by alleviating oxidative stress and the related metabolism dysfunction, inflammation, fibrosis, and tumorigenesis. The targeted signaling pathways may include, but are not limited to, NRF2, AMPK, SIRT1, NF-κB, TLR4/MYD88, TGF-β/SMAD, and PI3K/Akt/FoxO1, etc. In this review, we thoroughly discuss the oxidative stress-related mechanisms involved in NAFLD development, as well as summarize the protective effects and underlying mechanisms of green tea and EGCG against NAFLD.

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