Endometrial Cell Sampling Procedure to Obtain Cytologic Specimens

Author(s):  
Tetsuji Kurokawa ◽  
Toshimichi Onuma ◽  
Akiko Shinagawa ◽  
Yoshio Yoshida
Author(s):  
Vinci Mizuhira ◽  
Hiroshi Hasegawa

Microwave irradiation (MWI) was applied to 0.3 to 1 cm3 blocks of rat central nervous system at 2.45 GHz/500W for about 20 sec in a fixative, at room temperature. Fixative composed of 2% paraformaldehyde, 0.5% glutaraldehyde in 0.1 M cacodylate buffer at pH 7.4, also contained 2 mM of CaCl2 , 1 mM of MgCl2, and 0.1% of tannic acid for conventional observation; and fuether 30-90 mM of potassium oxalate containing fixative was applied for the detection of calcium ion localization in cells. Tissue blocks were left in the same fixative for 30 to 180 min after MWI at room temperature, then proceeded to the sampling procedure, after postfixed with osmium tetroxide, embedded in Epon. Ultrathin sections were double stained with an useal manner. Oxalate treated sections were devided in two, stained and unstained one. The later oxalate treated unstained sections were analyzed with electron probe X-ray microanalyzer, the EDAX-PU-9800, at 40 KV accelerating voltage for 100 to 200 sec with point or selected area analyzing methods.


1987 ◽  
Vol 58 (03) ◽  
pp. 834-838
Author(s):  
Knut Lande ◽  
Sverre Erik Kjeldsen ◽  
Ivar Eide ◽  
Paul Leren ◽  
Knut Gjesdal

SummaryBlood platelet function was evaluated in 10 men, all 50 years old, with untreated, mild hypertension. Each patient was examined four times: At the beginning of the study, after 5 weeks on placebo treatment, after the following 5 weeks on propranolol 160 mg daily, and finally after a second period of 5 weeks on placebo. At baseline the plasma level of the platelet release product (β-thromboglobulin (BTG) was 41.6 (30.5-57.0) μg/l (median and 95% confidence interval). During the first placebo period BTG was normalized to 21.0 (14.1-25.9) μg/l. While systolic blood pressure and heart rate fell during β-adrenergic receptor blockade, BTG remained unchanged throughout the rest of the observation periods. Platelet size increased significantly during treatment with β-blocker. The present study indicates that the normalization of elevated platelet function which previously has been reported to occur during anti-hypertensive drug therapy, may be explained by patient adaptation to the blood sampling procedure.


2019 ◽  
Vol 30 (5) ◽  
pp. 217-220

This report provides an overview of the 2017 official control activities on pesticide residues carried out in the European Union (EU) Member States, Iceland and Norway. It summarises the results of both the 2017 EU-coordinated control programme (EUCP) and the national control programmes (NP). While the NPs are mostly risk based (so called enforcement samples) focusing on pesticides or products originating from countries where a number of exceedances have been observed in the past, the EUCP aims to present a statistically representative snapshot of the situation of pesticide residues in food products that are mostly consumed in the EU following a random sampling procedure. The report includes the outcome of a dietary risk assessment based on the results of the overall 2017 control programmes. The comprehensive analysis of the results of all reporting countries provides risk managers with sound-based evidence for designing future monitoring programmes, in particular for taking decisions on which pesticides and food products should be targeted in risk-based national programmes.


2018 ◽  
Vol 30 (3) ◽  
pp. 167-173

Red colored lipstick is the most widely used cosmetic product. Although lipstick gives a lot of social, psychological and therapeutic benefits, it may harm the consumers. Because some lipsticks contain a considerable amount of heavy metal especially lead. Lead is being used in lipstick mainly for the pigments required to obtain needed colors. Lead accumulates in the body over time and lead-containing lipstick applied several times a day, every day, combined with lead in water and other sources, could add up to significant exposure levels. Therefore, this study was aimed to determine lead content in red colored lipsticks from market. This study was laboratorybased, analytical study by using 25 lipstick samples. Red colored lipsticks were bought from Mandalay Market by random sampling procedure and they were completely coded to avoid the bias. Then, lead content in coded samples was determined by Flame AAS according to International Conference on Harmonization (ICH) guideline. Lead contents of 88% of the lipsticks samples were more than specified limit (20 ppm) of Food and Drug Administration, United States. All of them, lead content was highest in counterfeit lipsticks group. Among the tested lipstick samples, lipstick with lowest lead content was LE-RL 01 (15.74 ppm) and the lipstick with highest lead content was CF-RL 01(60.09 ppm). In conclusion, lead contents of red colored lipsticks (22 out of 25) from market samples were higher than allowable limit (20 ppm).


2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Nia Agustiningsih ◽  
Setyawati Soeharto ◽  
Rinik Eko Kapti

Abstract : Patient with chronical diease such as Hemodialysis more likely need psycotherapy because until now medical atention only focus on physical aspect. Psycotherapy that can be used on Hemodialysis that going through depression is cognitive therapy and logo therapy. This study was to analysed the difference of influence between cognitive therapy and logo therapy on Hemodialysis patient that going through depression. This study was used quasi experimental pre–post test design. Respondent taking part in this study was 15 respondent for cognitive therapy and 15 respondent for logo therapy with puporsive sampling procedure. Study was conducted on Hemodialysis unit on RS Wava Husada Kepanjen from 21 April until 26 Mey 2017. Data analysis used on this study was dependent t test  and independent t test. Dependent t test  analysis showed  significant value is 0,000 (less than < 0,05).Analysis showed  significant value is 0,000 (less than < 0,05) that means there is difference between cognitive therapy and logo therapy to decrease depression on hemodialysis patient with mean value cognitive therapy (1,62) dan logo therapy (3,82). Conclussion on this study is that logo therapy more effective to reduce depression on Hemodialysis patient than cognitive therapy so logo therapy can applicated in hemodialysis patient.Keyword : cognitive therapy, logo therapy, depression in hemodialysis patient Abstrak : Pasien dengan penyakit kronis dengan hemodialisis diperlukan psikoterapi karena selama ini fokus penanganan di pelayanan kesehatan hanya pada masalah fisik. Psikoterapi yang bisa digunakan pada pasien hemodialisis yang mengalami depresi adalah cognitive therapy dan logo therapy. Penelitian ini bertujuan untuk menganalisis perbedaan pengaruh cognitive therapy dan logo therapy terhadap depresi pada pasien dengan hemodialisis. Penelitian ini menggunakan desain penelitian quasi experimental pre – post test design. Jumlah responden dalam penelitian ini 15 responden untuk kelompok cognitive therapy dan 15 responden untuk kelompok logo therapy yang didapatkan dengan menggunakan tehnik purposive sampling. Penelitian dilakukan di unit hemodialisis RS Wava Husada Kepanjen mulai tanggal 21 April – 26 Mei 2017. Pemberian terapi dilakukan oleh peneliti sendiri yang telah mendapatkan lisensi dari perawat spesialis jiwa. Analisis data yang digunakan dalam penelitian ini adalah dependent t test dan independent t test. Hasil analisis dependent t test  pada kelompok sebelum dan setelah diberikan cognitive therapy dan logo therapy didapatkan hasil nilai signifikansi 0,000 < 0,05 yang berarti bahwa ada perbedaan skor depresi antara sebelum dan sesudah diberikan pemberian cognitive therapy dan logo therapy  Sedangkan hasil analisis independent t test menunjukkan bahwa nilai signifikansi 0,000 < 0,05 yang berarti  ada perbedaan yang bermakna antara cognitive therapy dan logo therapy dalam menurunkan depresi pada pasien dengan hemodialisis yaitu dengan rata – rata penurunan terhadap depresi untuk cognitive therapy dan logo therapy masing – masing yaitu 1,62 dan 3,86.  Hal ini berarti bahwa logo therapy lebih efektif menurunkan depresi pada pasien hemodialisis dari pada cognitive therapy. Berdasarkan hasil penelitian tersebut diharapkan bahwa logo therapy bisa diaplikasikan sebagai alternatif psikoterapi pada pasien hemodialisis.Kata Kunci: cognitive therapy, logo therapy, depresi pada pasien hemodialisis


2020 ◽  
Author(s):  
Mahboobeh Farzin ◽  
Hassan Behzadi ◽  
Azam Sanatjoo ◽  
Soodabeh Shahidsaleth

BACKGROUND The information literacy treatment, which comprises an important part of the process of disease, is one of the aspects of health literacy and this concept has been explored in fewer studies. OBJECTIVE The purpose of the present study is to investigate the state of the components of information literacy treatment in Women cancer patients. METHODS This is an applied, survey-based study. The population consists of 143 Women cancer patients were selected employing a cluster random sampling procedure. Data were collected through a researcher-made questionnaire. The questionnaire includes 37 questions consisting of six categories—medications, treatment methods, nutrition, medical tests, treatment costs, and sport exercise. RESULTS The findings showed that patients had the most information needs in the five components (except sport exercise). In addition to, the most important source of information for patients was the doctor and, of course, some of the other medical staff. Also, all the identified criteria for cancer patients were important in using the information sources for treatment. The problems such as: specialized content, unfamiliarity with the medical terms, the complexity of the treatment process, are very important to the patients. CONCLUSIONS The health professionals play an important role in shortening the interval between the patients` need for information and receiving information. It is recommended for the health authorities to hold free classes in health centers and prepare simple and comprehensible materials in these courses.


Author(s):  
Dasari Tejaswini ◽  
Suhas Kulkarni ◽  
Dolar Doshi ◽  
Adepu Srilatha

AbstractBackgroundAesthetic alterations in the face can be self-perceived and can affect quality of life in young adults.ObjectiveTo assess the impact of malocclusion on self- perceived oro-facial behaviour among young adults.MethodsA Cross sectional study was conducted among 638 young adults (aged 18–21 years) of Hyderabad city. One college from each of the five zones of the Hyderabad city (five colleges) were selected by simple random sampling procedure. A 21-item Oro-facial investment scale (OFIS) questionnaire assessing self-perceived oral health knowledge, attitude, practice and Dental Aesthetic Index (DAI) assessing the severity of malocclusion was used. Data were analysed with standard statistical software (SPSS, Statistical package for the social sciences, version 20.0). p < 0.05 was considered statistically significant.ResultsMales had higher mean scores for knowledge (2.90 ± 0.49) than females (2.73 ± 0.82) and was statistically significant (p = 0.002*). No statistically significant gender differences were found in relation to attitude and practice. Analysis of variance (ANOVA) showed that there was a significant difference in all the three scores (knowledge, attitude, practice) with respect to age, with significantly high score for knowledge and practice among 18 year old subjects (p = 0.0001*; p = 0.0003* respectively) and attitude among 21 year old subjects (p = 0.0049*). No statistically significant age, gender differences were found in relation to DAI scores. Upon correlation, DAI significantly and positively correlated with knowledge (p = 0.03*) and attitude (p = 0.0001*).ConclusionThis study has shown significant impact of malocclusion on the self-perceived oro-facial behaviour.


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