Investigation of the oxygen tension in the brain tissues of albino rats after administration of radioprotective substances

This study concern to characterize the brain tissues to ischemic stroke, gray matter, white matter and CSF using texture analysisto extract classification features from CT images. The First Order Statistic techniques included sevenfeatures. To find the gray level variation in CT images it complements the FOS features extracted from CT images withgray level in pixels and estimate the variation of thesubpatterns. analyzing the image with Interactive Data Language IDL software to measure the grey level of images. The results show that the Gray Level variation and features give classification accuracy of ischemic stroke 97.6%, gray matter95.2%, white matter 97.3% and the CSF classification accuracy 98.0%. The overall classification accuracy of brain tissues 97.0%.These relationships are stored in a Texture Dictionary that can be later used to automatically annotate new CT images with the appropriate brain tissues names.

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Ameliorative Role of N-Acetylcysteine on Toxic Potential of Titanium Dioxide Nanoparticles on The Brain of Albino Rats

Mansoura Journal of Forensic Medicine and Clinical Toxicology ◽

10.21608/mjfmct.2014.47659 ◽

2014 ◽

Vol 22 (2) ◽

pp. 73-87

Author(s):

Shereen Elkhateeb ◽

Hossam Attia

Keyword(s):

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Albino Rats ◽

Toxic Potential ◽

The Brain

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Quercetin Attenuates Brain Oxidative Alterations Induced by Iron Oxide Nanoparticles in Rats

International Journal of Molecular Sciences ◽

10.3390/ijms22083829 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3829

Author(s):

Mohamed F. Dora ◽

Nabil M. Taha ◽

Mohamed A. Lebda ◽

Aml E. Hashem ◽

Mohamed S. Elfeky ◽

...

Keyword(s):

Iron Oxide ◽

B Cell Lymphoma ◽

Basal Diet ◽

Protective Role ◽

Iron Oxide Nanoparticle ◽

Albino Rats ◽

Peroxisome Proliferator ◽

Dependent Manner ◽

Peroxisome Proliferator Activated Receptor ◽

The Brain

Iron oxide nanoparticle (IONP) therapy has diverse health benefits but high doses or prolonged therapy might induce oxidative cellular injuries especially in the brain. Therefore, we conducted the current study to investigate the protective role of quercetin supplementation against the oxidative alterations induced in the brains of rats due to IONPs. Forty adult male albino rats were allocated into equal five groups; the control received a normal basal diet, the IONP group was intraperitoneally injected with IONPs of 50 mg/kg body weight (B.W.) and quercetin-treated groups had IONPs + Q25, IONPs + Q50 and IONPs + Q100 that were orally supplanted with quercetin by doses of 25, 50 and 100 mg quercetin/kg B.W. daily, respectively, administrated with the same dose of IONPs for 30 days. IONPs induced significant increases in malondialdehyde (MDA) and significantly decreased reduced glutathione (GSH) and oxidized glutathione (GSSG). Consequently, IONPs significantly induced severe brain tissue injuries due to the iron deposition leading to oxidative alterations with significant increases in brain creatine phosphokinase (CPK) and acetylcholinesterase (AChE). Furthermore, IONPs induced significant reductions in brain epinephrine, serotonin and melatonin with the downregulation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and mitochondrial transcription factor A (mtTFA) mRNA expressions. IONPs induced apoptosis in the brain monitored by increases in caspase 3 and decreases in B-cell lymphoma 2 (Bcl2) expression levels. Quercetin supplementation notably defeated brain oxidative damages and in a dose-dependent manner. Therefore, quercetin supplementation during IONPs is highly recommended to gain the benefits of IONPs with fewer health hazards.

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Protective role of protocatechuic acid in carbon tetrachloride-induced oxidative stress via modulation of proinflammatory cytokines levels in brain and liver of Wistar rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2020-0202 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Anne A. Adeyanju ◽

Folake O. Asejeje ◽

Olorunfemi R. Molehin ◽

Olatunde Owoeye ◽

Esther O. Olatoye ◽

...

Keyword(s):

Cholesterol Level ◽

Protocatechuic Acid ◽

Liver Toxicity ◽

Total Cholesterol Level ◽

Density Lipoprotein ◽

Albino Rats ◽

Antioxidant Enzyme Activities ◽

Cox 2 ◽

Anti Inflammatory ◽

The Brain

Abstract Objectives Protocatechuic acid (PCA) possesses numerous pharmacological activities, including antioxidative and anti-inflammatory activities. This study seeks to investigate its underlying mechanism of action in the liver and brain toxicity induced by CCl4 in male albino rats. Methods Rats were given PCA at 10 and 20 mg/kg daily and orally as a pretreatment for seven days. A single injection of CCl4 was given 2 h later to induce brain and liver toxicity. Results CCl4 moderately elevated the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). PCA lowered AST level significantly when compared to control. Total protein and albumin levels presented insignificant changes (p>0.05) in all groups while lipid profile showed increased total cholesterol level and reduced high-density lipoprotein (HDL) by CCl4. PCA (10 mg/kg) significantly reduced the cholesterol level while the 20 mg/kg dose moderately prevented HDL reduction. There was an increased MDA production with a corresponding low GSH level in the group treated with CCl4. Activities of superoxide dismutase, catalase, and glutathione-S-transferase in both organs also declined. PCA, especially at 10 mg/kg attenuated lipid peroxidation by increasing GSH level in the organs. Biochemical assays revealed the improvement of antioxidant enzyme activities by PCA in these organs. Furthermore, PCA lowered the level of proinflammatory cytokine COX 2 in the brain and liver while NF-kB expression was inhibited in the brain. Histopathology reports validated the effects of PCA. Conclusions PCA exhibited protection against toxicity in these tissues through antioxidant and anti-inflammatory activities and the potential mechanism might be through modulation of the NF-κB/COX-2 pathway.

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A fingerprint of amyloid plaques in a bitransgenic animal model of Alzheimer's disease obtained by statistical unmixing analysis of hyperspectral Raman data

The Analyst ◽

10.1039/c9an01631g ◽

2019 ◽

Vol 144 (23) ◽

pp. 7049-7056 ◽

Cited By ~ 4

Author(s):

Emerson A. Fonseca ◽

Lucas Lafetá ◽

Renan Cunha ◽

Hudson Miranda ◽

João Campos ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Animal Model ◽

Amyloid Plaques ◽

Model Of Alzheimer’S Disease ◽

The Brain ◽

Brain Tissues

We have found different Raman signatures of AB fibrils and in brain tissues from unmixed analysis, providing a detailed image of amyloid plaques in the brain, with the potential to be used as biomarkers.

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Proteomic analysis of the brain tissues from a transgenic mouse model of amyloid β oligomers

Neurochemistry International ◽

10.1016/j.neuint.2012.05.018 ◽

2012 ◽

Vol 61 (3) ◽

pp. 347-355 ◽

Cited By ~ 13

Author(s):

Masaoki Takano ◽

Kouji Maekura ◽

Mieko Otani ◽

Keiji Sano ◽

Tooru Nakamura-Hirota ◽

...

Keyword(s):

Mouse Model ◽

Transgenic Mouse ◽

Proteomic Analysis ◽

Amyloid Β ◽

Transgenic Mouse Model ◽

The Brain ◽

Brain Tissues

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ПОВЕДІНКОВА АКТИВНІСТЬ ЩУРІВ І РІВЕНЬ ЕНДОТОКСИКОЗУ МОЗКУ НА ТЛІ ГІДРАЗИНОВОГО ГЕПАТИТУ

Scientific Issue Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: Biology ◽

10.25128/2078-2357.19.2.13 ◽

2019 ◽

Vol 76 (2) ◽

pp. 78-84

Author(s):

O. A. Makarenko ◽

T. V. Hladkyi ◽

A. V. Maikova ◽

T. V. Mohylevska

Keyword(s):

Open Field ◽

Urease Activity ◽

Toxic Hepatitis ◽

Male Rats ◽

Behavioural Activity ◽

Animal Physiology ◽

Malondialdehyde Content ◽

Experimental Formation ◽

The Brain ◽

Brain Tissues

Hepatic encephalopathy is a frequent complication and manifestation of liver diseases, and a consequence of liver failure. Our research aims at studying behavioral and emotional activity, as well as identification of the degree of endotoxicosis of brain tissues of rats at the background of modelling in them of toxic chronic hydrazine hepatitis. The research was carried out at the Department of Human and Animal Physiology of Odessa National Mechnykov University on laboratory male rats, aged 8-10 months. The animals were divided into 2 groups, 6 animals in each, control (intact animals) and experimental (formation of a model of toxic hydrazine hepatitis) ones. The functional state of the brain of rats was evaluated by studying the behavioral and emotional activities of animals with the methods of "Open field" and "T- shaped labyrinth". In brain homogenates, the activity of a number of enzymes was determined, which could indicate the cause of changes in the functioning of the nervous system: the activity of lysozyme, urease, elastase, catalase, the content of malondialdehyde. It was discovered that formation of toxic hepatitis in rats is accompanied by inhibition of orientation and behavioural activity – on 50-70 %, exploratory – on 40-60 % and emotional – on 30 % in “open field” test, considerable aggravation of dynamics of rate and quality of learning problem solving in T-shaped labyrinth. Modeling of hepatitis in rats led to the decrease in lysozyme activity by 22.1%, catalase activity by 30.8%, detection of urease activity in the brain, as well as an increase in elastase activity by 44.6% and malondialdehyde content by 21.5%.в At the background of hepatitis in homogenates of brain tissues urease activity has been detected, activity of inflammation markers and enzymes-destructors increases, with activity of indices of antioxidant brain system decreasing. Change of behavioural activity of rats at the background of toxic hepatitis is caused by the development of endotoxicosis, which results from impairment of the function of liver detoxification.

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