Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH4 + concentrations in hydroponic conditions

Abstract Background The root system provides nutrient absorption and is closely related to abiotic stress tolerance, but it is difficult to study the roots under field conditions. This study was conducted to identify quantitative trait loci (QTL) associated with primary root length (PRL) during soybean seedling growth in hydroponic conditions. A total of 103 F7 recombinant inbred lines (RILs) derived from a cross between K099 (short primary root) and Fendou 16 (long primary root) were used to identify QTL for PRL in soybean. The RIL population was genotyped with 223 simple sequence repeats markers covering 20 chromosomes. Phenotyping for primary root length was performed for 3-weeks plants grown in hydoponic conditions. The identified QTL was validated in near isogenic lines and in a separate RIL population. Results QTL analysis using inclusive composite interval mapping method identified a major QTL on Gm16 between SSR markers Sat_165 and Satt621, explaining 30.25 % of the total phenotypic variation. The identified QTL, qRL16.1, was further confirmed in a segregating population derived from a residual heterozygous line (RHLs-98). To validate qRL16.1 in a different genetic background, QTL analysis was performed in another F6 RIL population derived from a cross between Union (medium primary root) and Fendou 16, in which a major QTL was detected again in the same genomic region as qRL16.1, explaining 14 % of the total phenotypic variation for PRL. In addition, the effect of qRL16.1 was confirmed using two pair of near-isogenic lines (NILs). PRL was significantly higher in NILs possessing the qRL16.1 allele from Fendou 16 compared to allele from K099. Conclusions The qRL16.1 is a novel QTL for primary root length in soybean which provides important information on the genetic control of root development. Identification of this major QTL will facilitate positional cloning and DNA marker-assisted selection for root traits in soybean.

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The Effect of Salicylic Acid and 20 Substituted Molecules on Alleviating Metolachlor Herbicide Injury in Rice (Oryza sativa)

Agronomy ◽

10.3390/agronomy10030317 ◽

2020 ◽

Vol 10 (3) ◽

pp. 317 ◽

Cited By ~ 5

Author(s):

Xile Deng ◽

Wenna Zheng ◽

Xiaomao Zhou ◽

Lianyang Bai

Keyword(s):

Salicylic Acid ◽

Oryza Sativa ◽

Root Length ◽

Plant Hormone ◽

Inhibitory Effect ◽

Pharmacological Effects ◽

Rice Seedlings ◽

Fresh Weight ◽

Wide Range ◽

Endogenous Plant

Salicylic acid (SA) is an endogenous plant hormone that has a wide range of pharmacological effects. Studies have indicated that SA has herbicide safening activity. In this study, the herbicide safening activity of SA and 20 substituted molecules were tested on agar-cultured rice. Biological assay results indicated that SA and substituted SA had a low inhibitory effect on the growth of rice seedlings (Oryza sativa), and partially alleviated the effects of metolachlor toxicity. Moreover, at 0.25 mg L−1, the safening effect of compounds l and u lessened the effects of metolachlor phytotoxicity on plant height and fresh weight when compared to the effects of the control, fenclorim. The effects of metolachlor toxicity were reduced on root length due to the safening effects of compounds l, n, and u; these effects were greater than those of fenclorim. These compounds could facilitate the development of novel herbicide safeners.

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Fine Mapping and Genetic Effect Analysis of qKRN5.04, a Major QTL Associ-ated with Kernel Row Number in Maize

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2017.00063 ◽

2017 ◽

Vol 43 (1) ◽

pp. 63 ◽

Cited By ~ 1

Author(s):

Na BAI ◽

Yong-Xiang LI ◽

Fu-Chao JIAO ◽

Lin CHEN ◽

Chun-Hui LI ◽

...

Keyword(s):

Fine Mapping ◽

Genetic Effect ◽

Effect Analysis ◽

Major Qtl ◽

Kernel Row Number

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Fine mapping TaFLW1, a major QTL controlling flag leaf width in bread wheat (Triticum aestivum L.)

Theoretical and Applied Genetics ◽

10.1007/s00122-013-2108-7 ◽

2013 ◽

Vol 126 (8) ◽

pp. 1941-1949 ◽

Cited By ~ 23

Author(s):

Shulin Xue ◽

Feng Xu ◽

Guoqiang Li ◽

Yan Zhou ◽

Musen Lin ◽

...

Keyword(s):

Triticum Aestivum ◽

Bread Wheat ◽

Fine Mapping ◽

Flag Leaf ◽

Triticum Aestivum L ◽

Leaf Width ◽

Flag Leaf Width ◽

Major Qtl

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Occurrence of plant growth-promoting traits in clover-nodulating rhizobia strains isolated from different soils in Rio Grande do Sul state

Revista Brasileira de Ciência do Solo ◽

10.1590/s0100-06832009000500016 ◽

2009 ◽

Vol 33 (5) ◽

pp. 1227-1235 ◽

Cited By ~ 21

Author(s):

Luciano Kayser Vargas ◽

Bruno Brito Lisboa ◽

Gilson Schlindwein ◽

Camille Eichelberger Granada ◽

Adriana Giongo ◽

...

Keyword(s):

Plant Growth ◽

Root Length ◽

Indoleacetic Acid ◽

Rio Grande ◽

Plant Growth Promoting Rhizobacteria ◽

Phytopathogenic Fungus ◽

Rio Grande Do Sul ◽

Rice Seedlings ◽

Plant Growth Promoting ◽

Growth Promoting

In the last decades, the use of plant growth-promoting rhizobacteria has become an alternative to improve crop production. Rhizobium leguminosarum biovar trifolii is one of the most promising rhizobacteria and is even used with non-legume plants. This study investigated in vitro the occurrence of plant growth-promoting characteristics in several indigenous R. leguminosarum biovar trifolii isolated from soils in the State of Rio Grande do Sul, Brazil. Isolates were obtained at 11 locations and evaluated for indoleacetic acid and siderophore production and inorganic phosphate solubilization. Ten isolates were also molecularly characterized and tested for antagonism against a phytopathogenic fungus and for plant growth promotion of rice seedlings. Of a total of 252 isolates, 59 produced indoleacetic acid, 20 produced siderophores and 107 solubilized phosphate. Some degree of antagonism against Verticillium sp. was observed in all tested isolates, reducing mycelial growth in culture broth. Isolate AGR-3 stood out for increasing root length of rice seedlings, while isolate ELD-18, besides increasing root length in comparison to the uninoculated control, also increased the germination speed index, shoot length, and seedling dry weight. These results confirm the potential of some strains of R. leguminosarum biovar trifolii as plant growth-promoting rhizobacteria.

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Identification and fine-mapping of a major QTL (qRtsc8-1) conferring resistance to maize tar spot complex and production markers validation in breeding lines

10.21203/rs.3.rs-1072295/v1 ◽

2021 ◽

Author(s):

Jiaojiao Ren ◽

Penghao Wu ◽

Gordon M. Huestis ◽

Ao Zhang ◽

Jingtao Qu ◽

...

Keyword(s):

Latin American ◽

Fine Mapping ◽

Genome Wide Association Study ◽

Selective Genotyping ◽

Doubled Haploid Population ◽

Phenotypic Variance ◽

Breeding Lines ◽

A Genome ◽

Tar Spot ◽

Major Qtl

Abstract Tar spot complex (TSC) is a major foliar disease of maize in many Central and Latin American countries and leads to severe yield loss. To dissect the genetic architecture of TSC resistance, a genome-wide association study (GWAS) panel and a bi-parental doubled haploid population were used for GWAS and selective genotyping analysis, respectively. A total of 115 SNPs in bin 8.03 were detected by GWAS and three QTL in bins 6.05, 6.07, and 8.03 were detected by selective genotyping. The major QTL qRtsc8-1 located in bin 8.03 was detected by both analyses, it explained 14.97% of the phenotypic variance. To fine-map qRtsc8-1, the recombinant-derived progeny test was implemented. Recombinations in each generation were backcrossed, and the backcross progenies were genotyped with Kompetitive Allele Specific PCR (KASP) markers and phenotyped for TSC resistance individually. The significant tests for comparing the TSC resistance between the two classes of progenies with and without resistant alleles were used for fine-mapping. In BC5 generation, qRtsc8-1 was fine mapped in an interval of ~721 kb flanked by markers of KASP81160138 and KASP81881276. In this interval, the candidate genes GRMZM2G063511 and GRMZM2G073884 were identified, which encode an integral membrane protein-like and a leucine-rich repeat receptor-like protein kinase, respectively. Both genes are involved in maize disease resistance responses. Two production markers KASP81160138 and KASP81160155 were verified in 471 breeding lines. This study provides valuable information for cloning the resistance gene, it will also facilitate the routine implementation of marker-assisted selection in the breeding pipeline for improving TSC resistance.

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Fine Mapping of a Major QTL Influencing Morphine Preference in C57BL/6 and DBA/2 Mice Using Congenic Strains

Neuropsychopharmacology ◽

10.1038/npp.2008.14 ◽

2008 ◽

Vol 33 (12) ◽

pp. 2801-2809 ◽

Cited By ~ 16

Author(s):

Glenn A Doyle ◽

Patrick J Furlong ◽

Candice L Schwebel ◽

George G Smith ◽

Falk W Lohoff ◽

...

Keyword(s):

Fine Mapping ◽

Congenic Strains ◽

Major Qtl

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Fine mapping of qBK1, a major QTL for bakanae disease resistance in rice

Rice ◽

10.1186/s12284-019-0295-9 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 5

Author(s):

Sais-Beul Lee ◽

Namgyu Kim ◽

Yeon-Jae Hur ◽

Su-Min Cho ◽

Tae-Heon Kim ◽

...

Keyword(s):

Disease Resistance ◽

Fine Mapping ◽

Bakanae Disease ◽

Major Qtl

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Fine mapping of the major QTL for seed coat color in Brassica rapa var. Yellow Sarson by use of NIL populations and transcriptome sequencing for identification of the candidate genes

PLoS ONE ◽

10.1371/journal.pone.0209982 ◽

2019 ◽

Vol 14 (2) ◽

pp. e0209982 ◽

Cited By ~ 6

Author(s):

Huiyan Zhao ◽

Urmila Basu ◽

Berisso Kebede ◽

Cunmin Qu ◽

Jiana Li ◽

...

Keyword(s):

Candidate Genes ◽

Brassica Rapa ◽

Fine Mapping ◽

Seed Coat ◽

Transcriptome Sequencing ◽

Coat Color ◽

Seed Coat Color ◽

Major Qtl

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Response of Selected Soybean (Glycine max) Cultivars to Dimethenamid and Metolachlor in Hydroponic Conditions

Weed Technology ◽

10.1017/s0890037x00023149 ◽

1995 ◽

Vol 9 (1) ◽

pp. 178-181 ◽

Cited By ~ 12

Author(s):

Bradley T. Osborne ◽

David R. Shaw ◽

Randall L. Ratliff

Keyword(s):

Glycine Max ◽

Root Length ◽

Lateral Root ◽

Lateral Roots ◽

Hydroponic Culture ◽

Soybean Cultivars ◽

Length Reduction ◽

Hydroponic Conditions ◽

Lateral Root Length

Soybean cultivars were grown in a hydroponic culture to evaluate tolerance to dimethenamid and metolachlor. Root length was reduced in 7 of the 32 cultivars with 50 ppb dimethenamid. ‘Asgrow 5403’ and ‘Bryan’ root lengths were reduced more than 20%. ‘Asgrow 6297’ was the only cultivar with greater than 12% root length reductions with 83 ppb metolachlor. The longest lateral root of all cultivars was shortened with dimethenamid. ‘NK-1460’ and Bryan expressed the most lateral root length reduction, at 73 and 62%, respectively. Asgrow 6297 was tolerant to dimethenamid, but lateral roots were reduced 16%. Metolachlor reduced the lateral root lengths of 12 cultivars, with Asgrow 5403, ‘Coker 6955,’ and Bryan reduced more than 20%. Bryan was the most sensitive cultivar to both herbicides, while ‘Brim’ was the most tolerant of cultivars evaluated.

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