scholarly journals Ionic gold demonstrates antimicrobial activity against Pseudomonas aeruginosa strains due to cellular ultrastructure damage

2021 ◽  
Author(s):  
Miguel Reyes Torres ◽  
Anthony J. Slate ◽  
Steven F. Ryder ◽  
Maliha Akram ◽  
Conrado Javier Carrascosa Iruzubieta ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Morphological Changes ◽  
Therapeutic Option ◽  
Cell Ultrastructure ◽  
Wound Management ◽  
Cellular Ultrastructure ◽  
Lowry Assay ◽  
Ionic Gold

AbstractDue to the ever-increasing rise of antimicrobial resistant (AMR) bacteria, the development of alternative antimicrobial agents is a global priority. The antimicrobial activity of ionic gold was explored against four Pseudomonas aeruginosa strains with different AMR profiles in order to determine the antimicrobial activity of ionic gold and elucidate the mechanisms of action. Disc diffusion assays (zone of inhibition: ZoI) coupled with minimum inhibitory/bactericidal concentrations (MIC/MBC) were conducted to determine the antimicrobial efficacy of ionic gold. Scanning electron microscopy (SEM) was used to visualise morphological changes to the bacterial cell ultrastructure. Strains with increased AMR were slower to grow which is likely a fitness cost due to the enhanced AMR activity. Although greater concentrations of ionic gold were required to promote antimicrobial activity, ionic gold demonstrated similar antimicrobial values against all strains tested. Lowry assay results indicated that protein leakage was apparent following incubation with ionic gold, whilst SEM revealed cellular ultrastructure damage. This study suggests that the application of ionic gold as an alternative antimicrobial is promising, particularly against AMR P. aeruginosa. The antimicrobial activity of ionic gold against P. aeruginosa could potentially be utilised as an alternative therapeutic option in wound management, an approach that could benefit healthcare systems worldwide.

scholarly journals VARIATIONS IN ANTIMICROBIAL ACTIVITY OF SYZYGIUM CUMINI OF DIFFERENT GEOGRAPHICAL LOCATIONS

2020 ◽  
pp. 1-6
Author(s):  
Pratibha ◽  
Nesari Tanuja ◽  
Ghildiyal Shivani ◽  
Vandhana
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Streptococcus Pyogenes ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Syzygium Cumini ◽  
Geographical Variations ◽  
Geographical Locations

The emergence of antibiotic resistance and the evolution of new strains of disease causing agents, are of highly concern to the global health community. Plants are potential source of antimicrobial agents. They have been used traditionally for prevention of infections caused by micro-organisms. Description of Krimighana herbs enumerated in Ayurveda classics is suggestive towards the importance of this group of medicine. Jambu (Syzygium cumini (L.) Skeels) is a member of Myrtaceae family. In Raja Nighantu it is mentioned that plant Jambu is having Kriminashaka property. It has been widely used medicine in the prevention of various ailments like cough, Dysentary, Diabetes, inflammation and ringworm. It is well established fact that geographical variations effects the potential and activity of medicinal herbs. Hence, the present study was undertaken to investigate Syzygium cumini procured from different geographical locations including Delhi, Rajasthan and Maharashtra for their potential activity against human infections caused by pathogens. Method The aqueous extract of Syzygium cumini of all the three areas was prepared. The activity of the plant extract was evaluated against nine bacterial pathogens and one fungal strain, which include Staphyllococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Candida albicans. The activity was carried out using Disk diffusion method. Result and Conclusion: All samples of Syzygium cumini showed potential antimicrobial activity against four pathogens including Staphyllococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Candida albicans. MIC was also evaluated against the tested pathogenic strains. The sample from Maharashtra showed MIC i.e. 80µg, 40µg, 80µg against Staphyllococcus aureus, Streptococcus pyogenes and Candida albicans respectively which is less as compare to sample from Rajasthan and Delhi. Region wise sample from Maharashtra showed good ZOI and MIC.

Synthesis and characterization of PCU@C-Ag/AgCl nanoparticles as an antimicrobial material for respiratory tract infection

2021 ◽  
Vol 6 (1) ◽  
pp. 68-78
Author(s):  
Senthilkumar Palanisamy ◽  
Kalaivani Subramanian ◽  
Lerince Godrina Bennet ◽  
Janani Ambrose ◽  
Aganiya Gopalakrishnan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Antimicrobial Agents ◽  
Morphological Changes ◽  
Visible Spectrum ◽  
Ag Nps ◽  
Antimicrobial Material ◽  
Agcl Nanoparticles

Abstract The pregnant cow urine (PCU) is an active source of antimicrobial agents that is used for fabricating chitosan coated Ag/AgCl nanoparticles (NPs) in the present study. These PCU@C-Ag/AgCl NPs were physicochemically characterized and evaluated for antimicrobial activity against selected respiratory tract infection (RTI) pathogens. The absorption band around 420 nm in UV-Visible spectrum indicated the presence of Ag NPs. The spherical shape of NPs was observed using TEM. Also, the crystalline structure was confirmed using the XRD pattern. The PCU@C-Ag/AgCl NPs showed strong antimicrobial activity against all tested RTI pathogens. In addition, FESEM analysis showed morphological changes in RTI bacterial pathogens. Thereby, PCU@C-Ag/AgCl NPs may be used as an antimicrobial material to treat RTIs in near future at clinical level.

scholarly journals Synthesis and biological evaluation of some new 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines as antimicrobial agents

2022 ◽  
Vol 11 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Iryna Myrko ◽  
Taras Chaban ◽  
Vasyl Matiychuk
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Bacterial Strains ◽  
Synthesis And Biological Evaluation

A series of some new pyrazole-substituted 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines was synthesized in this study. The structures of target substances were confirmed by using 1H and 13С NMR spectroscopy, mass spectrometry and elemental analysis. The synthesized compounds have been evaluated for antimicrobial activity against five bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus) and two fungal strains (Candida albicans and Cryptococcus neoformans). The antimicrobial screening studies of synthesized substances established that 2 of 12 compounds show pronounced antibacterial activity against the strain Staphylococcus aureus.

scholarly journals In-vitro antibacterial activity of sequential crude extracts from Datura stramonium seeds

2016 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Powar Priyatama V ◽  
Powar Trupti A
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Agents ◽  
Datura Stramonium ◽  
Seed Extract ◽  
Zone Of Inhibition

The growing phenomenon of antibiotic resistance, particularly to pathogenic microorganisms, in current medicine, has directed the concern of scientists for finding novel antimicrobial agents from plant origin with negligible side effect. The present study was aimed to phytochemical investigation and antimicrobial activity of seed extract of Datura stramonium in sequentially with different organic solvents. For this, antimicrobial properties were tested against bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and Bacillus subtilis by cup plate method. Among the tested bacterial Klebsiella pneumoniae was the most inhibited majorly with the chloroform extract. Datura stramonium chloroform seed extract produced maximum zone of inhibition 26 mm against Klebsiella pneumoniae and 12 mm against Bacillus subtilis and 13 mm against Escherichia coli. Datura Stramonium methanol seed extract produced maximum zone of inhibition 27 mm against Pseudomonas aeruginosa and 15 mm against Bacillus subtilis, 14 mm against Staphylococcus aureus and 19 mm against Escherichia coli. Datura stramonium petroleum ether seed extract produced 16 mm zone of inhibition against Escherichia coli. Datura stramonium aqueous seed extract exhibits 24 mm zone of inhibition against Bacillus subtilis. All the experienced solvent extracts showed potential antimicrobial activity Index against various tested microorganisms. Owning to the results, it can be concluded that the extracts of the Datura stramonium can be used to design different herbal antimicrobial agents.

scholarly journals Invitro antibacterial activity of Cassia fistula Linn methanolic leaf extracts

2019 ◽  
Vol 8 (2) ◽  
pp. 270
Author(s):  
Bindhu R. Kamath ◽  
Sabeena Kizhedath
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cassia Fistula ◽  
Leaf Extracts ◽  
E Coli

Background: Cassia fistula Linn is a plant which is widely grown in India and is used for medicinal purposes. The study was carried out with an objective to demonstrate the antimicrobial activity of leaves of Cassia fistula Linn. The aim of the study is to assess antibacterial and antifungal activity of methanolic leaf extract of Cassia fistula Linn against selected clinical isolates.Methods: The antimicrobial activity of methanolic extract of Cassia fistula was evaluated using agar well diffusion method and to zone of inhibition of extract was determined. Clinical isolates of Staphyloccocus aureus, MRSA, Pseudomonas aeruginosa, E. coli and Proteus were screened.Results: The methanolic extracts exhibited antibacterial activity against Staphylococcus aureus. The extract was not active against E. coli, Proteus, MRSA, Pseudomonas aeruginosa. The extract also failed to demonstrate antifungal activity against Candida albicans and Aspergillus niger.Conclusions: The global emergence of multidrug resistant bacterial strains is increasing, limiting the effectiveness of current drugs and treatment failure of infections. A novel approach to the prevention of antibiotic resistance of pathogenic species is the use of new compounds that are not based on existing synthetic antimicrobial agents.

scholarly journals Nickel (II) and Cobalt (II) Complexes of Some Amino Acid-based Surfactants with Antimicrobial Activity

2019 ◽  
Vol 70 (2) ◽  
pp. 361-364
Author(s):  
Dana Varasteanu ◽  
Irina Chican ◽  
Sanda Maria Doncea ◽  
Iuliana Raut ◽  
Mariana Calin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Amino Acid ◽  
Complex Formation ◽  
Bacillus Cereus ◽  
Aspergillus Flavus ◽  
Antimicrobial Agents ◽  
Cobalt Complexes ◽  
Bacteria And Fungi

Nickel and cobalt complexes of lauroyl-glycine, lauroyl-glycylglycine and 1,12-dodecandioyl-diglycylglycine were synthesized. The FTIR spectroscopy confirmed the complex formation. The antimicrobial activity of synthesized nickel and cobalt complexes was investigated against several bacteria and fungi: Bacillus cereus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus flavus. Generally, the nickel and cobalt complexes of the investigated surfactants showed no antimicrobial activity on Pseudomonas aeruginosa, a moderate one on Bacillus cereus and Aspergillus flavus. The most susceptible microorganism was Candida albicans, which is considered one of most common nosocomial infections. The results suggest the potential application of tested compounds as antimicrobial agents against targeted pathogens.

scholarly journals Composition and Antimicrobial Activity of the Essential Oils of Three Plant Species from the Sabana of Bogota (Colombia): Myrcianthes leucoxyla, Vallea stipularis and Phyllanthus salviifolius

2016 ◽  
Vol 11 (12) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Luis Miguel Pombo Ospina ◽  
Paola Borrego Muñoz ◽  
Javier Matulevich ◽  
Aníbal Alfonso Teherán ◽  
Leidy Barajas Villamizar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Caryophyllene Oxide ◽  
Alternative Source ◽  
Zones Of Inhibition ◽  
Microbial Strains ◽  
Positive Controls

In recent decades, essential oils (EOs) have become an important alternative source of antimicrobial agents. The objective of this study was to evaluate the antimicrobial activity of EOs obtained from the leaves of Myrcianthes leucoxyla Mc. Vaughn., Vallea stipularis L.f and Phyllanthus salviifolius Kunth for prevalent microbial strains and also to contribute to the knowledge of their chemical composition. EOs were obtained by hydrodistillation and analyzed by GC-MS. The main compounds for V. stipularis EO were α-tujene (6.6%) and cedrene epoxide (12.0%), for M. leucoxila EO 1,8 cineole (6.3%) and caryophyllene oxide (21.7%), and for P. salviifolius aristolene (17.3%) and geranyl tiglate (15.9%). Antimicrobial tests showed that M. leucoxyla EO was the most active, showing zones of inhibition against all strains used, specifically against Pseudomonas aeruginosa and Salmonella typhimurium with percentages of inhibition higher than 50%. Compared with positive controls, this activity may be related to the presence of 1,8 cineole.

scholarly journals A PRELIMINARY OBSERVATION ON AN EXPLICIT ANTIMICROBIAL ACTION OF MANGROVE PLANTS ON PSEUDOMONAS AERUGINOSA

2019 ◽  
pp. 226-230
Author(s):  
TAMANNA SULTANA ◽  
ARUP KUMAR MITRA ◽  
SATADAL DAS
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Microtiter Plate ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Mangrove Plant ◽  
E Coli ◽  
Mangrove Plants

Objectives: Due to emerging drug-resistant microorganisms throughout the world, newer antimicrobial agents should be looked for. Plants are enriched with different bioactive chemicals. In this study, we searched antibacterial activities of some mangrove plant extracts against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Methods: In the present study, the antimicrobial activity of the leaves of Bruguiera gymnorhiza, Excoecaria agallocha, Avicennia alba, and Aegialitis rotundifolia was evaluated against a few reference pathogenic bacterial strains, namely, P. aeruginosa ATCC 27853, E. coli ATCC 25922, S. aureus ATCC 29213, and multidrug-resistant bacterial strains E. coli extended-spectrum beta-lactamases strain. Aqueous, ethanolic, methanolic, and dimethyl sulfoxide (DMSO) extracts were studied. The antimicrobial activities of the organic solvent extracts on the various test microorganisms were investigated using agar well diffusion technique followed by determination of minimum inhibitory concentration values by serial dilution in a microtiter plate. Results: Ethanol and DMSO extracts of B. gymnorhiza exhibited promising antimicrobial activity followed by extracts of A. alba and E. agallocha. Among all microorganisms studied, P. aeruginosa ATCC 27853 showed significant growth inhibition with ethanol and DMSO extracts. Conclusion: Extracts of some mangrove plants, particularly, B. gymnorhiza showed very good antimicrobial activities against common microbial agents causing human infections and in general mangrove plants appear to act better on P. aeruginosa.

scholarly journals Graphene Matrices as Carriers for Metal Ions against Antibiotic Susceptible and Resistant Bacterial Pathogens

Coatings ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 352
Author(s):  
Anthony J. Slate ◽  
Nathalie Karaky ◽  
Grace S. Crowther ◽  
Jonathan A. Butler ◽  
Craig E. Banks ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Metal Ions ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Therapeutic Option ◽  
Bacterial Strains ◽  
Graphene Foam ◽  
Coating Method ◽  
Resistant Strains ◽  
Specific Metal

Due to the ever-increasing burden of antimicrobial-resistant (AMR) bacteria, the development of novel antimicrobial agents and biomaterials to act as carriers and/or potentiate antimicrobial activity is essential. This study assessed the antimicrobial efficacy of the following ionic metals, silver, gold, palladium, platinum, zinc, and gallium alone and in combination with graphene matrices (which were coated via a drop casting coating method). The graphene foam was utilized as a carrier for the ionic metals against both, antibiotic susceptible and resistant bacterial strains of Acinetobacter baumannii,Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. Ionic gold, palladium and platinum demonstrated the greatest antimicrobial activity against the susceptible and resistant strains. Scanning electron microscopy (SEM) visualized cellular ultrastructure damage, when the bacteria were incubated upon the graphene foam alone. This study suggests that specific metal ions applied in combination with graphene foam could present a potential therapeutic option to treat AMR bacterial infections. The application of the graphene foam as a potential carrier could promote antimicrobial activity, provide a sustained release approach and reduce possible resistance acquisition. In light of this study, the graphene foam and ionic metal combinations could potentially be further developed as part of a wound dressing.

scholarly journals Synthesis and Evaluation of Some New Thiazolidin-4-One Derivatives as Potential Antimicrobial Agents

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
B. A. Baviskar ◽  
S. S. Khadabadi ◽  
S. L. Deore
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Salmonella Typhi ◽  
Zone Of Inhibition ◽  
Structure Activity

A new series ofN-{4-methyl-5-[4-(4-oxo-2-phenyl(1,3-thiazolidin-3-yl)]-5-sulfanyl(1,2,4-triazol-3-yl)-1,3-thiazol-2-yl }acetamide (7a-l) was synthesized in order to determine their antimicrobial activity and feasible structure–activity relationships. The compounds were synthesized in good yield and the structures of all newly synthesized compounds were established on the basis of their IR,1HNMR, and elemental analysis. The synthesized compounds were testedin vitroantibacterial activity againstStaphylococcus aureus,Escherichia coli,Pseudomonas aeruginosaandSalmonella typhiand antifungal activity againstAspergillus niger,Candida albicansby measuring the zone of inhibition in mm.

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