Targeting Staphylococcus aureus and its biofilms with novel antibacterial compounds produced by Lactiplantibacillus plantarum SJ33

Due to the prevalence of thioamides in antibacterial compounds, we chose to convert the amide in the antitubercular compound 2-(decylsulfonyl)acetamide to a thioamide using Lawesson’s reagent to study its activity against a range of microorganisms. This derivative (8) had significantly diminished activity against tuberculosis but slightly better activity than the parent compound against the Gram positive species Staphylococcus aureus. This activity against a second major pathogen is remarkable considering the structural simplicity of these compounds.

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Phosphonopeptides Revisited, in an Era of Increasing Antimicrobial Resistance

Molecules ◽

10.3390/molecules25061445 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1445 ◽

Cited By ~ 1

Author(s):

Emma C.L. Marrs ◽

Linda Varadi ◽

Alexandre F. Bedernjak ◽

Kathryn M. Day ◽

Mark Gray ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Resistance ◽

Enterococcus Faecalis ◽

Antibacterial Agents ◽

Methicillin Resistant Staphylococcus Aureus ◽

Peptide Mimetics ◽

Antibacterial Resistance ◽

Bacterial Isolates ◽

New Agents ◽

Antibacterial Compounds

Given the increase in resistance to antibacterial agents, there is an urgent need for the development of new agents with novel modes of action. As an interim solution, it is also prudent to reinvestigate old or abandoned antibacterial compounds to assess their efficacy in the context of widespread resistance to conventional agents. In the 1970s, much work was performed on the development of peptide mimetics, exemplified by the phosphonopeptide, alafosfalin. We investigated the activity of alafosfalin, di-alanyl fosfalin and β-chloro-L-alanyl-β-chloro-L-alanine against 297 bacterial isolates, including carbapenemase-producing Enterobacterales (CPE) (n = 128), methicillin-resistant Staphylococcus aureus (MRSA) (n = 37) and glycopeptide-resistant enterococci (GRE) (n = 43). The interaction of alafosfalin with meropenem was also examined against 20 isolates of CPE. The MIC50 and MIC90 of alafosfalin for CPE were 1 mg/L and 4 mg/L, respectively and alafosfalin acted synergistically when combined with meropenem against 16 of 20 isolates of CPE. Di-alanyl fosfalin showed potent activity against glycopeptide-resistant isolates of Enterococcus faecalis (MIC90; 0.5 mg/L) and Enterococcus faecium (MIC90; 2 mg/L). Alafosfalin was only moderately active against MRSA (MIC90; 8 mg/L), whereas β-chloro-L-alanyl-β-chloro-L-alanine was slightly more active (MIC90; 4 mg/L). This study shows that phosphonopeptides, including alafosfalin, may have a therapeutic role to play in an era of increasing antibacterial resistance.

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Potent and Non-Cytotoxic Antibacterial Compounds Against Methicillin-Resistant Staphylococcus aureus Isolated from Psiloxylon mauritianum, A Medicinal Plant from Reunion Island

Molecules ◽

10.3390/molecules25163565 ◽

2020 ◽

Vol 25 (16) ◽

pp. 3565

Author(s):

Jonathan Sorres ◽

Amandine André ◽

Elsa Van Elslande ◽

Didier Stien ◽

Véronique Eparvier

Keyword(s):

Staphylococcus Aureus ◽

Medicinal Plant ◽

Biological Activities ◽

Reunion Island ◽

Minimal Inhibition Concentration ◽

Antibiotic Resistant ◽

Antibacterial Compounds ◽

Methicillin Resistant ◽

Réunion Island ◽

Endemic Medicinal Plant

With the occurrence of antibiotic-resistant Staphylococcus aureus strains, identification of new anti-staphylococcal drugs has become a necessity. It has long been demonstrated that plants are a large and diverse source of antibacterial compounds. Psiloxylon mauritianum, an endemic medicinal plant from Reunion Island, was chemically investigated for its reported biological activity against S. aureus. Aspidin VB, a phloroglucinol derivative never before described, together with Aspidin BB, were first isolated from the ethyl acetate extract of P. mauritianum leaves. Their structures were elucidated from spectroscopic data. Aspidin VB exhibited strong antibacterial activity against standard and methicillin-resistant S. aureus strains, with a minimal inhibition concentration (MIC) of 0.25 μg/mL, and no cytotoxicity was observed at 10−5 M in MRC5 cells. Due to its biological activities, Aspidin VB appears to be a good natural lead in the fight against S. aureus.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TANAMAN JOHAR (Cassia siamea Lamk.) DARI BEBERAPA TINGKAT KEPOLARAN PELARUT

KOVALEN ◽

10.22487/j24775398.2017.v3.i3.9333 ◽

2017 ◽

Vol 3 (3) ◽

pp. 242

Author(s):

Fitriah Fitriah ◽

Mappiratu Mappiratu ◽

Prismawiryanti Prismawiryanti

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Leaf Extract ◽

Micrococcus Luteus ◽

Solvent Polarity ◽

Shigella Dysenteriae ◽

Diffusion Method ◽

Antibacterial Compounds ◽

Cassia Siamea ◽

Activity Test

A research about antibacterial activity test of johar plant leaf extract (Cassia siamea Lamk.) using several levels of solvent polarity has been implemented. This study aims to determine whether the antibacterial compounds in the leaves is polar, non-polar or semi-polar, and to determine the highest inhibition level of johar leaf extract on both the gram-negative bacteria (Escherichia coli and Shigella dysenteriae) and gram-positive (Staphylococcus aureus and Micrococcus luteus). The maceration method was used in this research with 3 levels of solvent polarity namely non-polar, semi-polar and polar. The bacterial activity test was done with diffusion method. The results showed that the johar leaf contains antibacterial compounds which are polar and semi-polar. The highest inhibition level was obtained at johar leaf ethanol extract for Staphylococcus aureus, Escherichia coli, Micrococcus luteu and Shigella dysentriae of 14.9, 12.9, 12, and 7.2 mm, respectively.Keywords: Antibacterial, Inhibition Level, Johar Leaf Extract

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New antibacterial compounds inhibiting Staphylococcus aureus enoyl-ACP reductase from Penicillium sp.

Planta Medica ◽

10.1055/s-0035-1556395 ◽

2015 ◽

Vol 81 (11) ◽

Author(s):

N Kim ◽

MJ Shon ◽

H Koshino ◽

WG Kim

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Compounds ◽

Penicillium Sp ◽

Enoyl Acp Reductase

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A Staphylococcus aureus Fitness Test Platform for Mechanism-Based Profiling of Antibacterial Compounds

Chemistry & Biology ◽

10.1016/j.chembiol.2009.07.004 ◽

2009 ◽

Vol 16 (8) ◽

pp. 826-836 ◽

Cited By ~ 69

Author(s):

Robert G.K. Donald ◽

Stephen Skwish ◽

R. Allyn Forsyth ◽

Jennifer W. Anderson ◽

Tanya Zhong ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Compounds ◽

Fitness Test ◽

Test Platform

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FORMULASI DAN UJI EFEKTIVITAS KRIM ANTIBAKTERI EKSTRAK DAUN NANGKA (Artocarpus heterophyllus Lam.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽

10.35799/pha.8.2019.29257 ◽

2019 ◽

Vol 8 (1) ◽

pp. 225

Author(s):

Nurul S Majid ◽

Paulina V. Y. Yamlean ◽

Gayatri Citraningtyas

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Physical Stability ◽

Ethanol Extract ◽

Inhibition Zone ◽

Quality Test ◽

Antibacterial Compounds ◽

Artocarpus Heterophyllus ◽

Test Parameters

ABSTRACT Jackfruit leaves contain saponins, flavonoids and tannins. Which are know to have antibacterial compounds. The purpose of this study is to make cream of jackfruit leaf extract with a concentration of 5%, 10% and 15% and test the quality of preparations of jackfruit leaf extract cream and there is antibacterial effectiveness against Staphylococcus aureus bacteria. The method used is the ethanol extract of Jackfruit leaves by formulating it as an M/A type cream. The results showed the cream of jackfruit leaf extract fulfilled all the physical stability tests of the cream and in the antibacterial testing of cream of Jackfruit leaf extract could inhibit the effectiveness of Staphylococcus aureus bacteria. As concluded, the Jackfruit leaf extract Conclusions obtained by jackfruit leaf extract can be formulated as a cream with a concentration of 5%, 10% and 15%, and cream preparations meet the cream quality test parameters, for testing the effectiveness of antibacterial to the biggest inhibition zone Staphylococcus aureus is 10.5 mm at 15% concentration. Keywords: Jackfruit leaves, cream antibacterial, Staphylococcus aureus. ABSTRAK Daun Nangka memiliki kandungan saponin, flavonoid dan tannin yang berperan sebagai senyawa antibakteri. Tujuan penelitian ini yaitu membuat krim ekstrak daun Nangka dengan konsentrasi 5%, 10% dan 15%, menguji mutu dan efektivitas antibakteri krim ekstrak daun Nangka terhadap bakteri Staphylococcus aureus. Metode yang digunakan yaitu ekstrak etanol daun Nangka dengan memformulasikan sebagai krim dengan tipe M/A. Hasil penelitian menunjukkan krim ekstrak daun Nangka memenuhi semua uji kestabilan fisik krim dan dalam pengujian antibakteri krim ekstrak daun Nangka dapat menghambat efektivitas bakteri Staphylococcus aureus. Kesimpulan yang diapat ekstrak daun Nangka dapat diformulasikan sebagai krim dengan konsentrasi 5%, 10% dan 15%, dan sediaan krim memenuhi parameter uji kualitas krim, untuk pengujian efektivitas antibakteri terhadap Staphylococcus aureus zona hambat terbesar yaitu 10,5 mm pada konsentrasi 15%. Kata kunci: Daun Nangka, krim antibakteri, Staphylococcus aureus.

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Antibacterial Activity of Cinnamon Extract (Cinnamomum burmannii) against Staphylococcus aureus and Escherichia coli In Vitro

BioScientia Medicina ◽

10.32539/bsm.v3i2.85 ◽

2019 ◽

Vol 3 (2) ◽

pp. 19-28

Author(s):

Nita Parisa ◽

Rahma Nur Islami ◽

Ella Amalia ◽

Mariana Mariana ◽

Riana Sari Puspita Rasyid

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethanol Extract ◽

Inhibition Zone ◽

Control Group ◽

Antibacterial Compounds ◽

Cinnamon Extract ◽

Cinnamomum Burmannii

Abstract Infectious disease is one of the most common diseases in the world. Staphylococcus aureus and Escherichia coli are two common causes of infection and are resistant to many antibiotics, so the new agents are needed to overcome antibiotic resistance. Cinnamon is often used as a preservative because it has antibacterial activity. Cinnamomum burmannii is kind of native cinnamon from Indonesia. The antimicrobial active compounds cinnamaldehyde and eugenol are the main reasons for its antibacterial activity. This study observed the efficacy of the cinnamon extract (Cinnamomum burmannii) as antibacterial against Staphylococcus aureus and Escherichia coli. An experimental study, in vitro using Post-test Only Control Group Designed, has been done in Microbiology and Biotechnology Laboratory of Medical Faculty of Sriwijaya University. Cinnamon was extracted, then tested for its antibacterial activity using well diffusion and serial dilution to determine diameter of inhibition zone and minimum bactericidal concentration. Phytochemical tests were also conducted to determine the antibacterial compounds of cinnamon extract. Ethanol extract of cinnamon was able to inhibit the growth of Staphylococcus aureus with MBC 5% and inihibitory zone 6,84Â±0,68 mm and Escherichia coli with MBC 10% and inhibitory zone 5,69Â±0,69 mm. Cinnamon extract which has the greatest effectiveness is concentration of 40% with inhibition zone 15,69Â±0,80 mm (Staphylococcus aureus) and 9,63Â±0,59 mm (Escherichia coli). This ability is due to the antibacterial compounds as evidenced by positive results in various phytochemical tests. Cinnamon extract is effective as antibacterial against Staphylococcus aureus and Escherichia coli in vitro. Keywords: efficacy, antibacterial, Cinnamomum burmannii, Staphylococcus aureus, Escherichia coli

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Senyawa Antibakteri Escherichia coli ATCC 35218 dan Staphylococcus aureus ATCC 25923 dari Kapang Endofit Taman Nasional Gunung Halimun

Jurnal Natur Indonesia ◽

10.31258/jnat.12.1.21-27 ◽

2012 ◽

Vol 12 (1) ◽

pp. 21 ◽

Cited By ~ 1

Author(s):

Ruth Melliawati ◽

Harni Harni

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Retention Time ◽

Endophytic Fungus ◽

High Performance ◽

Agar Plate ◽

Chloroform Fraction ◽

Antibacterial Compounds

Endophytic fungus is a microorganism which live in the interstitial spaces healthy tissues of the host plant, andhas capability to produce secondary metabolite such as micotoxin, antibiotic and antiviral. This research wasaimed to find out the isolates of endophytic fungus produce antibacterial compounds to inhibit Escerichia coliATCC 35218 and Staphylococcus aureus ATCC 25923, and to investigate the Retention time (Rt) of the antibacterialcompounds produced by endophytic fungus with High Performance Liquid Chromatography (HPLC) methods.Diffusion Agar Plate Method was used to examine the antibacterial compounds on Escerichia coli ATCC 35218 andStaphylococcus aureus ATCC 25923. While the antibacterial compounds were examined with Thin LayerChromatography (TLC) and High Performance Liquid Chromatography (HPLC) methods, and the result werecompared with Chloramphenicol and Ampicillin antibiotic standart. Two isolates of endophytic fungus namelyHl.46F.211 and HL.57F.258 were inhibited the growth Escherichia coli ATCC 35218 and three isolates namelyHL.48F217, HL.53F.243 and HL.57F.258 showed antagonistic action against Staphylococcus aureus ATCC 25923.The results of TLC and HPLC analysing method show that antibacterial compounds produced by endophytic fungusHL.46F.211 had Rt (Retention Time) rate similar with antibiotic Chloramphenicol at 2,796 (at water fraction) and Rtantibiotic Amphycillin at 2,662 (at Chloroform fraction). While antibacterial compounds produced by endophyticfungus HL.57F.258 had Rt rate similar with antibiotic Amphycillin at 2,650 (at Chloroform fraction).

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Isolasi dan Karakterisasi Bakteri Endofit dari Tanaman Binahong dan Katepeng China

Jurnal Kesehatan Andalas ◽

10.25077/jka.v3i2.33 ◽

2014 ◽

Vol 3 (2) ◽

Author(s):

Desriani Desriani ◽

Ukhradia Maharaniq Safira ◽

Maria Bintang ◽

Akhmad Rivai ◽

Puspita Lisdiyanti

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Cereus ◽

Endophytic Bacteria ◽

Pathogenic Bacteria ◽

Clear Zone ◽

Antibacterial Compounds ◽

Cassia Alata ◽

Potential Activity ◽

Medical Effect

AbstrakBakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman.Beberapa studi menunjukkan bahwa bakteri endofit tertentu dapat memproduksi senyawa kimia yang memiliki efek bagi kesehatan, terutama bakteri endofit yang diisolasi dari tanaman obat.Binahong (Anredera cordifolia) dan Ketepeng Cina (Cassia alata) merupakan tanaman obat yang telah diketahui memiliki beberapa khasiat.Tujuan dari penelitian ini ialah mengisolasi dan mengkarakterisasi bakteri endofit dari kedua tanaman tersebut melalui uji antibakteriterhadap tiga jenis bakteri patogen manusia (Pseudomonas aeruginosa, Staphylococcus aureus, dan Bacillus cereus). Metode: Jumlah bakteri endofit yang berhasil diisolasi dari dua tanaman tersebut berjumlah 73 isolat (37 isolat dari Binahong dan 36 isolat dari Ketepeng Cina). Hasil: Berdasarkan uji antibakteri terhadap tiga bakteri patogen diperoleh 16 isolat bakteri endofit yang memiliki aktivitas potensial (ditandai dengan terbentuknya zona hambat), yaitu 1 isolat dari Ketepeng Cina (terhadap Pseudomonas aeruginosa) dan 15 isolat dari Binahong. Pembahasan: Terbentuknya zona hambat mengindikasikan kemungkinan adanya senyawa kimia yang memiliki efek antibakteri.Kata kunci: bakteri endofit, Anredera cordifolia, Cassia alata, antibakteri.AbstractEndophytic bacteria is a beneficial microorganism that interacts with plant without causing any harm to the host. Most studies showed that certain endophytic bacteria can produce chemical compound which have medical effect, especially endophytic bacteria isolated from medicinal plant. Binahong (Anrederacordifolia) and KetepengCina (Cassia alata) are several of medicinal plants potential to be used for medical purpose. Methods: The objective of this study was to isolate and to characterize endophytic bacteria from both plants through antibacterial activity test against to three kinds of human pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus). Results: total isolated endophytic bacteria from both plants are 73 isolates (37 isolates from Binahong and 36 isolates from KetepengCina). There were 16 isolates have potential activity (indicated by clear zone), one isolate from KetepengCina and 15 isolates from Binahong. Discussion: The formed clear zone indicates antibacterial compounds produced by endophyticbacteriatested. Further research is needed to identify the type of antibacterial compounds produced.Keywords:endophytic bacteria, Anrederacordifolia, Cassia alata, antibacterial.

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