scholarly journals Mass spectrometry profiling and quantitation of changes in circulating hormones secreted over time in Cancer borealis hemolymph due to feeding behavior

2021 ◽  
Author(s):  
Kellen DeLaney ◽  
Mengzhou Hu ◽  
Wenxin Wu ◽  
Michael P. Nusbaum ◽  
Lingjun Li
Keyword(s):  
Mass Spectrometry ◽  
Feeding Behavior ◽  
Cancer Borealis ◽  
Over Time

Analysis of Squalene and Its Transformation By-Products in Latent Fingermarks by Ultrahigh-Pressure Liquid Chromatography-High Resolution Accurate Mass Orbitrap™ Mass Spectrometry

2019 ◽  
Author(s):  
Buddhika Dorakumbura ◽  
Francesco Busetti ◽  
Simon Lewis
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Storage Conditions ◽  
Ultrahigh Pressure ◽  
Accurate Mass ◽  
Rational Approach ◽  
By Products ◽  
Over Time ◽  
High Resolution Accurate Mass

<p>Transformation of squalene and its by-products in fingermarks over time under different storage conditions (light, dark and underwater) was examined through ultrahigh-pressure liquid chromatography high resolution accurate mass Orbitrap™ mass spectrometry. Complications of assessing fingermark compositional variation over time using multiple samples with varying initial compositions were elucidated and a more rational approach was successfully demonstrated. Squalene was detected in all fresh natural fingermarks and the amount ranged between 0.20 to 11.32 μg/5 fingertips. A notable difference in the transformation of squalene was observed with different storage conditions, where a dark aquatic environment accelerated degradation of squalene compared to dark but dry conditions. Squalene monohydroperoxide was extremely short-lived in natural deposits while the amount of squalene epoxide was still increasing relative to the initial amount, after ageing under dark and aquatic conditions for up to 7 days. Some oxidation by-products of cholesterol were also tentatively identified, which exhibited a growth over time against their initial concentration under any of the storage condition tested. These by-products, therefore, show potential as biomarkers for targeted visualisation of aged deposits.</p>

scholarly journals Detection of Acetaminophen and Its Glucuronide in Fingerprint by SALDI Mass Spectrometry Using Zeolite and Study of Time-Dependent Changes in Detected Ion Amount

Analytica ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 66-75
Author(s):  
Toshiki Horikoshi ◽  
Chihiro Kitaoka ◽  
Yosuke Fujii ◽  
Takashi Asano ◽  
Jiawei Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Glucuronic Acid ◽  
Laser Desorption ◽  
The Body ◽  
Time Dependent ◽  
Laser Desorption Ionization ◽  
Fingerprint Analysis ◽  
Desorption Ionization ◽  
Acid Conjugate ◽  
Over Time

The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H]+ peak started later than that of [AAP+H]+ peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis.

scholarly journals Mass Spectrometry Quantification, Localization, and Discovery of Feeding-Related Neuropeptides in Cancer borealis

2021 ◽  
Vol 12 (4) ◽  
pp. 782-798
Author(s):  
Kellen DeLaney ◽  
Mengzhou Hu ◽  
Tessa Hellenbrand ◽  
Patsy S. Dickinson ◽  
Michael P. Nusbaum ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Borealis

Nocturnal patterns of macronutrient intake in freely feeding and food-deprived rats

1989 ◽  
Vol 256 (2) ◽  
pp. R541-R548 ◽  
Author(s):  
D. L. Tempel ◽  
G. Shor-Posner ◽  
D. Dwyer ◽  
S. F. Leibowitz
Keyword(s):  
Feeding Behavior ◽  
Food Deprivation ◽  
Nutrient Intake ◽  
Macronutrient Intake ◽  
Feeding Response ◽  
Feeding Patterns ◽  
Carbohydrate Ingestion ◽  
Compensatory Feeding ◽  
Nutrient Selection ◽  
Over Time

Analyses of rats' feeding behavior at the start and the end of the nocturnal cycle have revealed dramatic alterations in macronutrient intake over time. At dark onset, rats displayed a preference for carbohydrate, with the first meal of the night consisting of approximately 60% of this nutrient. This carbohydrate intake was soon followed by a shift toward protein-predominant meals. Superimposed on this pattern of meal-to-meal shifts in nutrient selection appears to be an additional rhythm in which carbohydrate ingestion was favored at dark onset and protein and fat ingestion were favored during the late dark hours. Differential feeding patterns were also apparent following mild food deprivation. A 2-h period of deprivation at dark onset produced a strong compensatory feeding response, particularly of fat and carbohydrate. This pattern was not observed at the end of the dark, when little compensatory feeding was demonstrated. It is suggested that these feeding patterns may be related to the activity of certain hypothalamic neurotransmitters, e.g., norepinephrine and serotonin, known to be important in modulating temporal feeding patterns and nutrient intake.

scholarly journals High Mannose N-Glycans Promote Migration of Bone-Marrow-Derived Mesenchymal Stromal Cells

2020 ◽  
Vol 21 (19) ◽  
pp. 7194
Author(s):  
Vivian Alonso-Garcia ◽  
Cutter Chaboya ◽  
Qiongyu Li ◽  
Bryan Le ◽  
Timothy J. Congleton ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Percutaneous Injection ◽  
Secretion Profile ◽  
Migration Potential ◽  
High Mannose ◽  
Over Time

For hundreds of indications, mesenchymal stromal cells (MSCs) have not achieved the expected therapeutic efficacy due to an inability of the cells to reach target tissues. We show that inducing high mannose N-glycans either chemically, using the mannosidase I inhibitor Kifunensine, or genetically, using an shRNA to silence the expression of mannosidase I A1 (MAN1A1), strongly increases the motility of MSCs. We show that treatment of MSCs with Kifunensine increases cell migration toward bone fracture sites after percutaneous injection, and toward lungs after intravenous injection. Mechanistically, high mannose N-glycans reduce the contact area of cells with its substrate. Silencing MAN1A1 also makes cells softer, suggesting that an increase of high mannose N-glycoforms may change the physical properties of the cell membrane. To determine if treatment with Kifunensine is feasible for future clinical studies, we used mass spectrometry to analyze the N-glycan profile of MSCs over time and demonstrate that the effect of Kifunensine is both transitory and at the expense of specific N-glycoforms, including fucosylations. Finally, we also investigated the effect of Kifunensine on cell proliferation, differentiation, and the secretion profile of MSCs. Our results support the notion of inducing high mannose N-glycans in MSCs in order to enhance their migration potential.

scholarly journals A Review of the Radiocarbon Dates for the Afanasyevo Culture (Central Asia): Shifting Towards the “Shorter” Chronology

Radiocarbon ◽  
2018 ◽  
Vol 61 (1) ◽  
pp. 243-263 ◽  
Author(s):  
Andrey V Poliakov ◽  
Svetlana V Svyatko ◽  
Nadezhda F Stepanova
Keyword(s):  
Mass Spectrometry ◽  
Accelerator Mass Spectrometry ◽  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Scintillation Counting ◽  
Radiocarbon Dates ◽  
Archaeological Data ◽  
Depth Analysis ◽  
Over Time ◽  
Ams Dates

ABSTRACTThis article provides a summary and in-depth analysis of all existing radiocarbon (14C) dates for the Afanasyevo Culture of the Paleometal period. The previous “long” chronology of the culture was widely criticized and contradicted many archaeological observations. The exceedingly wide ranges of the liquid scintillation counting (LSC) dates from bone samples produced in several laboratories and the systematically older ages for the wood/charcoal samples finally reveal the shortcomings of the conventional “long” chronology. From accelerator mass spectrometry (AMS), the Afanasyevo burials of the Altai are dated to the 31st–29th century BC, whereas those of the Middle Yenisei Region to the 29th–25th century BC, which confirms the relatively earlier age of the Altai monuments. The “short” chronology removes the incompatibility of deriving the Afanasyevo Culture from the Yamnaya Culture, which previously appeared “younger” than the Afanasyevo, and also contradictions with the archaeological data. It also explains the small number of sites, the small size of the cemeteries and the lack of the internal periodization. We can now clearly move, from the earlier understanding that the Afanasyevo chronology is too broad, towards a different perception. The new AMS dates only represent a “core” for the Afanasyevo chronology, which cannot be narrowed down, but could be slightly expanded over time.

scholarly journals Capillary electrophoresis coupled to MALDI mass spectrometry imaging with large volume sample stacking injection for improved coverage of C. borealis neuropeptidome

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Kellen DeLaney ◽  
Lingjun Li
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Mass Spectrometry Imaging ◽  
Model Organism ◽  
Laser Desorption ◽  
Maldi Mass Spectrometry ◽  
Laser Desorption Ionization ◽  
Cancer Borealis ◽  
Sample Stacking ◽  
Ms Imaging

This work reports on a capillary electrophoresis (CE) separation method coupled to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) imaging for improved neuropeptide coverage in the model organism Cancer borealis.

scholarly journals Episodic Bouts of Activity Accompany Recovery of Rhythmic Output By a Neuromodulator- and Activity-Deprived Adult Neural Network

2003 ◽  
Vol 90 (4) ◽  
pp. 2720-2730 ◽  
Author(s):  
Jason A. Luther ◽  
Alice A. Robie ◽  
John Yarotsky ◽  
Christopher Reina ◽  
Eve Marder ◽  
...  
Keyword(s):  
Neural Network ◽  
Neuronal Network ◽  
Recovery Process ◽  
Stomatogastric Ganglion ◽  
Cancer Borealis ◽  
Pyloric Network ◽  
Underlying Mechanisms ◽  
Over Time ◽  
Phase Relationships ◽  
Pyloric Rhythm

The pyloric rhythm of the stomatogastric ganglion of the crab, Cancer borealis, slows or stops when descending modulatory inputs are acutely removed. However, the rhythm spontaneously resumes after one or more days in the absence of neuromodulatory input. We recorded continuously for days to characterize quantitatively this recovery process. Activity bouts lasting 40–900 s began several hours after removal of neuromodulatory input and were followed by stable rhythm recovery after 1–4 days. Bout duration was not related to the intervals (0.3–800 min) between bouts. During an individual bout, the frequency rapidly increased and then decreased more slowly. Photoablation of back-filled neuromodulatory terminals in the stomatogastric ganglion (STG) neuropil had no effect on activity bouts or recovery, suggesting that these processes are intrinsic to the STG neuronal network. After removal of neuromodulatory input, the phase relationships of the components of the triphasic pyloric rhythm were altered, and then over time the phase relationships moved toward their control values. Although at low pyloric rhythm frequency the phase relationships among pyloric network neurons depended on frequency, the changes in frequency during recovery did not completely account for the change in phase seen after rhythm recovery. We suggest that activity bouts represent underlying mechanisms controlling the restructuring of the pyloric network to allow resumption of an appropriate output after removal of neuromodulatory input.

scholarly journals Strategies for understanding dynamic, personalized profiles of host-derived proteins and microbes from human stool

2019 ◽  
Author(s):  
Ellen Casavant ◽  
Les Dethlefsen ◽  
Kris Sankaran ◽  
Daniel Sprockett ◽  
Susan Holmes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Profile ◽  
Host Protein ◽  
Host Proteins ◽  
Protein Marker ◽  
Gastrointestinal Health ◽  
Wide Range ◽  
Subject Variability ◽  
Human Stool ◽  
Over Time

AbstractMeasuring host proteins through noninvasive stool-based assays opens new avenues for characterizing states of gastrointestinal health. However, the extent to which these proteins vary over time and between healthy subjects is poorly characterized. Here, we characterize technical and biological sources of variability in mass spectrometry-based measurements of host proteins in stool. We identify the proteins that most vary over time within an individual, and among different individuals. Finally, we examine and compare temporal and inter-individual variation in host protein and bacterial taxonomic profiles of the same fecal specimens. To address these issues, five self-reported healthy individuals were each sampled eight times over four weeks. First, we demonstrate that mass spectrometry-based identification and label-free quantification of stool proteins exhibit non-significant variability (p>0.05) between both technical and preparative replicates for a subset of 78 proteins, supporting the utility of this method for biomarker measurement. Second, although 13 human stool proteins varied significantly in relative abundance over time within individuals, 58 proteins varied significantly (at least four-fold) between subjects. The average pair-wise difference between individuals was greater than the average within-subject difference for both the proteome and microbiome datasets (p<0.0001). Fecal host proteins, like the traditional fecal protein marker, calprotectin, unambiguously pointed to innate and adaptive immune responses. For example, one subject’s fecal protein profile suggested a sub-clinical inflammatory state. From these data, we conclude that host-centric protein measurements in stool reveal a wide range of variation during states of apparent health, and add a valuable complementary insight into host-microbiota relationships.IMPORTANCEHuman proteins in stool hold untapped potential for characterizing gastrointestinal health. To fully harness this potential and create a baseline of healthy stool protein abundances and identifications, it will be important to establish the extent to which these proteins might vary in the absence of disease. This study quantifies the major sources of variation in stool protein abundance data. We assessed technical, preparative, temporal, and inter-subject variability of human protein abundances in stool and found that among these sources, differences between subjects accounted for the greatest amount of variation, followed by temporal differences, and then technical factors. Our paired microbiome analysis found matching patterns of temporal and inter-subject variability. By characterizing multiple variance parameters in host stool protein abundances, our analysis helps to contextualize a wide range of future disease-focused stool studies as well as elucidate host-microbe interactions.

scholarly journals Effect of Mechanical and Moisture-stress Conditioning on Growth and Cuticle Composition of Broccoli Transplants

1997 ◽  
Vol 122 (6) ◽  
pp. 788-791 ◽  
Author(s):  
Joyce G. Latimer ◽  
Ray F. Severson
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Plant Growth ◽  
Methylene Chloride ◽  
Moisture Stress ◽  
Epicuticular Waxes ◽  
Greenhouse Production ◽  
Detached Leaves ◽  
Stress Conditioning ◽  
Over Time

Epicuticular waxes were analyzed to explain the visible differences in the waxy bloom of conditioned broccoli (Brassica oleracea L. Group Italica `Green Duke') transplants. Seedlings (22 days old) were subjected to brushing (40 cycles per minute, 1 minute twice daily), wind (7 m·s-1 for 5 minutes twice daily), or moisture-stress conditioning (MSC; visible wilt for 2 to 4 hours daily) for 16 (1987) or 21 (1988) days during transplant production in the greenhouse. The epicuticular waxes of the uppermost fully expanded leaves were removed by dipping detached leaves into methylene chloride. The extract was derivatized with trimethylsilyl reagents and subjected to capillary gas chromatography. The primary epicuticular wax components were the nonpolar C29 compounds nonacosane, nonacosan-15-ol, and nonacosan-15-one, which were identified by mass spectrometry. In a Summer 1987 experiment, cuticle samples taken over time of treatment indicated acclimation to the conditioning treatments relative to untreated plants. After 9 days of treatment, the amount of total epicuticular waxes present on the leaves was reduced 38%, 31%, or 11% by wind, brushing, or MSC, respectively. However, after 15 days of treatment, the amount of cuticle present was reduced 15% by brushing but only 6% by wind and was 17% greater in MSC-treated plants. Two weeks after transplanting to the field there were no differences in the amount or composition of the epicuticular waxes. In Fall 1988, all treatments reduced plant growth, but only MSC tended to increase the amount of C29 epicuticular components during greenhouse production. Differences in the amounts of epicuticular waxes were no longer significant after 8 days in the field.

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