Does near-infrared (NIR) fluorescence angiography modify operative strategy during emergency procedures?

Background. Indocyanine green (ICG) with near-infrared (NIR) fluorescence is an established method for assessing vascularity in various clinical settings. We hypothesized that parathyroid adenomas, with increased capillary networks, may demonstrate a fluorescence which could aid intraoperative identification and confirmation of the abnormal parathyroid tissue. Methods. This prospective case–control study compared patients with primary hyperparathyroidism undergoing parathyroidectomy (cases) to normal parathyroid in thyroidectomy patients (controls). After exposing the parathyroid gland, ICG was injected and the fluorescence of parathyroid and thyroid was recorded and graded in comparison to the surrounding tissue and vasculature (0 = nonfluorescent and 5 = vasculature). Results. The intensity of parathyroid fluorescence was more in cases (4 ± 2) than controls (2 ± 1) when graded intraoperatively ( P = .001). Thyroid fluorescence did not differ (3 vs 3, P = .072); however, parathyroid fluorescence was more intense than thyroid in cases (parathyroid = 4 ± 2 and thyroid = 3 ± 1, P = .018). Conclusions. ICG fluorescence in diseased parathyroid was more intense than normal parathyroid and thyroid, suggesting the ICG/NIR technology may be a useful intraoperative tool for identification of abnormal parathyroid.

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Shortwave Infrared Fluorescence Imaging with the Clinically Approved Near-Infrared Dye Indocyanine Green

10.1101/100768 ◽

2017 ◽

Cited By ~ 7

Author(s):

Jessica A. Carr ◽

Daniel Franke ◽

Justin R. Caram ◽

Collin F. Perkinson ◽

Vasileios Askoxylakis ◽

...

Keyword(s):

Indocyanine Green ◽

Real Time ◽

Fluorescence Imaging ◽

Near Infrared ◽

Infrared Region ◽

Fluorescence Angiography ◽

Nir Fluorescence ◽

Near Infrared Dye ◽

Shortwave Infrared

AbstractFluorescence imaging is a method of real-time molecular tracking in vivo that has enabled many clinical technologies. Imaging in the shortwave infrared region (SWIR, 1-2 μm) promises higher contrast, sensitivity, and penetration depths compared to conventional visible and near-infrared (NIR) fluorescence imaging. However, adoption of SWIR imaging in clinical settings has been limited, due in part to the absence of FDA-approved fluorophores with peak emission in the SWIR. Here, we show that commercially available NIR dyes, including the FDA-approved contrast agent indocyanine green (ICG), exhibit optical properties suitable for in vivo SWIR fluorescence imaging. Despite the fact that their emission reaches a maximum in the NIR, these dyes can be imaged non-invasively in vivo in the SWIR spectral region, even beyond 1500 nm. We demonstrate real-time fluorescence angiography at wavelengths beyond 1300 nm using ICG at clinically relevant doses. Furthermore, we show tumortargeted SWIR imaging with trastuzumab labeled with IRDye 800CW, a NIR dye currently being tested in multiple phase II clinical trials. Our findings suggest that high-contrast SWIR fluorescence imaging can be implemented alongside existing imaging modalities by switching the detection of conventional NIR fluorescence systems from silicon-based NIR cameras to emerging indium gallium arsenide (InGaAs) SWIR cameras. Using ICG in particular opens the possibility of translating SWIR fluorescence imaging to human clinical applications.

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Infrared fluorescence lymphography in experimental and clinical practice

Regional blood circulation and microcirculation ◽

10.24884/1682-6655-2018-17-2-84-91 ◽

2018 ◽

Vol 17 (2) ◽

pp. 84-91 ◽

Cited By ~ 1

Author(s):

G. V. Papayan ◽

A. L. Akopov ◽

P. A. Antonyan ◽

A. A. Ilin ◽

N. N. Petrishchev

Keyword(s):

Lymph Nodes ◽

Near Infrared ◽

Lymphatic System ◽

Lymphatic Vessels ◽

Diagnostic System ◽

Biological Tissues ◽

Peritumoral Injection ◽

Intradermal Administration ◽

Nir Fluorescence ◽

Real Time Visualization

Introduction. Near infrared (NIR) fluorescent diagnostics is promising due to a deeper penetration into biological tissues. Material and methods. In experiments on rabbits and in clinical studies evaluation the lymphatic system with the use of the instrument complex FLUM-808 was analysed. Results. For visualization of the lymphatic vessels of the skin, the intradermal administration of ICG, dissolved in 20 % albumin in the order of 0.02 mg/ml, is optimal. Peritumoral injection of ICG allows visualizing sentinel lymph nodes in patients with lung cancer. Conclusions. The developed NIR fluorescence diagnostic system FLUM-808 allows to real time visualization of lymphatic vessels and lymph nodes.

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Near-infrared fluorescence imaging with indocyanine green for quantification of changes in tissue perfusion following revascularization

Vascular ◽

10.1177/17085381211032826 ◽

2021 ◽

pp. 170853812110328

Author(s):

Pim Van den Hoven ◽

Floris S Weller ◽

Merel Van De Bent ◽

Lauren N Goncalves ◽

Melissa Ruig ◽

...

Keyword(s):

Indocyanine Green ◽

Fluorescence Imaging ◽

Fluorescence Intensity ◽

Near Infrared ◽

Tissue Perfusion ◽

Control Group ◽

Maximum Slope ◽

Nir Fluorescence ◽

Significant Difference ◽

Revascularization Procedures

Objectives Current diagnostic modalities for patients with peripheral artery disease (PAD) mainly focus on the macrovascular level. For assessment of tissue perfusion, near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) seems promising. In this prospective cohort study, ICG NIR fluorescence imaging was performed pre- and post-revascularization to assess changes in foot perfusion. Methods ICG NIR fluorescence imaging was performed in 36 patients with PAD pre- and post-intervention. After intravenous bolus injection of 0.1 mg/kg ICG, the camera registered the NIR fluorescence intensity over time on the dorsum of the feet for 15 min using the Quest Spectrum Platform®. Time-intensity curves were plotted for three regions of interest (ROI): (1) the dorsum of the foot, (2) the forefoot, and (3) the hallux. Time-intensity curves were normalized for maximum fluorescence intensity. Extracted parameters were the maximum slope, area under the curve (AUC) for the ingress, and the AUC for the egress. The non-treated contralateral leg was used as a control group. Results Successful revascularization was performed in 32 patients. There was a significant increase for the maximum slope and AUC egress in all three ROIs. The most significant difference was seen for the maximum slope in ROI 3 (3.7%/s to 6.6%/s, p < 0.001). In the control group, no significant differences were seen for the maximum slope and AUC egress in all ROIs. Conclusions This study shows the potential of ICG NIR fluorescence imaging in assessing the effect of revascularization procedures on foot perfusion. Future studies should focus on the use of this technique in predicting favorable outcome of revascularization procedures.

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Gold nanoparticle-enhanced near infrared fluorescent nanocomposites for targeted bio-imaging

RSC Advances ◽

10.1039/c4ra12066c ◽

2015 ◽

Vol 5 (1) ◽

pp. 20-26 ◽

Cited By ~ 22

Author(s):

Hao Cheng ◽

Chuanxi Wang ◽

Zhenzhu Xu ◽

Huihui Lin ◽

Chi Zhang

Keyword(s):

Folic Acid ◽

Cancer Cells ◽

Gold Nanoparticle ◽

Near Infrared ◽

Water Solubility ◽

Imaging Agents ◽

Nir Fluorescence ◽

Target Imaging ◽

Bio Imaging ◽

Low Toxicity

Folic acid-conjugated nanocomposites with NIR fluorescence, water-solubility, and low toxicity are prepared and used as target-imaging agents for cancer cells.

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Near-Infrared Fluorescence Imaging and Photodynamic Therapy for Liver Tumors

Frontiers in Oncology ◽

10.3389/fonc.2021.638327 ◽

2021 ◽

Vol 11 ◽

Author(s):

Masaki Kaibori ◽

Hisashi Kosaka ◽

Kosuke Matsui ◽

Morihiko Ishizaki ◽

Hideyuki Matsushima ◽

...

Keyword(s):

Photodynamic Therapy ◽

Fluorescence Imaging ◽

Near Infrared ◽

Liver Tumors ◽

Nir Fluorescence ◽

Recent Developments ◽

Fluorescent Agent ◽

Stable Particles ◽

And Function ◽

Cancer Tissues

Surgery with fluorescence equipment has improved to treat the malignant viscera, including hepatobiliary and pancreatic neoplasms. In both open and minimally invasive surgeries, optical imaging using near-infrared (NIR) fluorescence is used to assess anatomy and function in real time. Here, we review a variety of publications related to clinical applications of NIR fluorescence imaging in liver surgery. We have developed a novel nanoparticle (indocyanine green lactosome) that is biocompatible and can be used for imaging cancer tissues and also as a drug delivery system. To date, stable particles are formed in blood and have an ~10–20 h half-life. Particles labeled with a NIR fluorescent agent have been applied to cancer tissues by the enhanced permeability and retention effect in animals. Furthermore, this article reviews recent developments in photodynamic therapy with NIR fluorescence imaging, which may contribute and accelerate the innovative treatments for liver tumors.

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Machine learning enables discovery of DNA-carbon nanotube sensors for serotonin

10.1101/2021.08.20.457145 ◽

2021 ◽

Author(s):

Payam Kelich ◽

Sanghwa Jeong ◽

Nicole Navarro ◽

Jaquesta Adams ◽

Xiaoqi Sun ◽

...

Keyword(s):

Machine Learning ◽

Carbon Nanotube ◽

Dna Sequences ◽

Regression Models ◽

Near Infrared ◽

Optical Response ◽

Support Vector ◽

Single Walled Carbon Nanotube ◽

Nir Fluorescence ◽

Selective Evolution

AbstractDNA-wrapped single walled carbon nanotube (SWNT) conjugates have remarkable optical properties leading to their use in biosensing and imaging applications. A critical limitation in the development of DNA-SWNT sensors is the current inability to predict unique DNA sequences that confer a strong analyte-specific optical response to these sensors. Here, near-infrared (nIR) fluorescence response datasets for ~100 DNA-SWNT conjugates, narrowed down by a selective evolution protocol starting from a pool of ~1010 unique DNA-SWNT candidates, are used to train machine learning (ML) models to predict new unique DNA sequences with strong optical response to neurotransmitter serotonin. First, classifier models based on convolutional neural networks (CNN) are trained on sequence features to classify DNA ligands as either high response or low response to serotonin. Second, support vector machine (SVM) regression models are trained to predict relative optical response values for DNA sequences. Finally, we demonstrate with validation experiments that integrating the predictions of ensembles of the highest quality CNN classifiers and SVM regression models leads to the best predictions of both high and low response sequences. With our ML approaches, we discovered five new DNA-SWNT sensors with higher fluorescence intensity response to serotonin than obtained previously. Overall, the explored ML approaches introduce an important new tool to predict useful DNA sequences, which can be used for discovery of new DNA-based sensors and nanobiotechnologies.

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Optical Probe for Near-Infrared (NIR) Fluorescence Signal Detection with High Optical Performance and Thermal Stability

Journal of Biomedical Science and Engineering ◽

10.4236/jbise.2014.710078 ◽

2014 ◽

Vol 07 (10) ◽

pp. 792-798 ◽

Cited By ~ 1

Author(s):

In Hee Shin ◽

Joo Beom Eom ◽

Jae Seok Park ◽

Hyeong Ju Park ◽

Byeong-Il Lee

Keyword(s):

Thermal Stability ◽

Signal Detection ◽

Near Infrared ◽

Optical Probe ◽

Fluorescence Signal ◽

Optical Performance ◽

Nir Fluorescence

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NIR-Emitting Benzene-Fused Oligo-BODIPYs for Bioimaging

10.33774/chemrxiv-2021-nl537 ◽

2021 ◽

Author(s):

Gabriele Selvaggio ◽

Robert Nißler ◽

Peter Nietmann ◽

Atanu Patra ◽

Lukas Jacek Patalag ◽

...

Keyword(s):

Particle Tracking ◽

Near Infrared ◽

Bathochromic Shift ◽

Visible Region ◽

Tissue Penetration ◽

Actin Networks ◽

Nir Dyes ◽

Nir Fluorescence ◽

J Aggregates ◽

20 Nm

Near-infrared (NIR) fluorophores are emerging tools for biophotonics because of their reduced scattering, increased tissue penetration and low phototoxicity. However, the library of NIR fluorophores is still limited. Here, we report the NIR fluorescence of two benzene-fused oligo-BODIPYs in their hexameric (H) and octameric (O) forms. These dyes emit bright NIR fluorescence (H: maxima 943/1075 nm, O: maxima 976/1115 nm) that can be excited in the NIR (H = 921 nm, O = 956 nm) or non-resonantly over a broad range in the visible region. The emission bands of H show a bathochromic shift and peak sharpening with increasing dye concentration suggesting the presence of J-aggregates. Furthermore, the emission maxima of both H and O shift up to 20 nm in solvents of different polarity. These dyes can be used as NIR ink and imaged remotely on the macroscopic level with a stand-off distance of 20 cm. We furthermore demonstrate their versatility for biophotonics by coating microscale beads and performing microrheology via NIR video particle tracking (NIR-VPT) in biopolymer (F-actin) networks. No photodamaging of the actin filaments takes place, which is typically observed for visible fluorophores and highlights the advantages of these NIR dyes.

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Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging

Journal of Marine Science and Engineering ◽

10.3390/jmse8010053 ◽

2020 ◽

Vol 8 (1) ◽

pp. 53

Author(s):

Thomas Oh ◽

Jittiwat Sermsripong ◽

Barry W. Hicks

Keyword(s):

Fluorescence Imaging ◽

Large Scale ◽

Near Infrared ◽

Fluorescent Proteins ◽

Fluorescence Emission ◽

Florida Keys ◽

Nir Fluorescence ◽

Novel Method ◽

Near Infrared Fluorescence Imaging

Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments. Here, we report a novel method of selectively imaging chlorophyll distributions in coral in situ using only the near infrared (NIR) fluorescence emission from chlorophyll. Commercially available equipment was assembled that allowed the sequential imaging of visible, visible-fluorescent, and NIR-fluorescent pigments on the same corals. The relative distributions of chlorophyll and fluorescent proteins (GFPs) were examined in numerous corals in the Caribbean Sea, the Egyptian Red Sea, the Indonesian Dampier Strait, and the Florida Keys. Below 2 m depth, solar induced NIR chlorophyll fluorescence can be imaged in daylight without external lighting, thus, it is much easier to do than visible fluorescence imaging done at night. The distributions of chlorophyll and GFPs are unique in every species examined, and while there are some tissues where both fluorophores are co-resident, often tissues are selectively enriched in only one of these fluorescent pigments. Although laboratory studies have clearly shown that GFPs can be photo-protective, their inability to prevent large scale bleaching events in situ may be due to their limited tissue distribution.

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