Exogenous melatonin mitigates chromium toxicity in maize seedlings by modulating antioxidant system and suppresses chromium uptake and oxidative stress

Background. Handball is considered an intermittent sport that places an important stress on a player's aerobic and anaerobic metabolism. However, the oxidative stress responses following a handball game remain unknown. We investigated the responses of plasma and erythrocyte antioxidant system and oxidative stress biomarkers following a single handball game.Methods. Fourteen male elite Brazilian handball athletes were recruited in the present study. Blood samples were taken before, immediately, and 24 hours after the game.Results. After the game and during 24 hours of recovery, the concentration of all oxidative stress indices changed significantly in a way indicating increased oxidative stress in the blood (thiol groups and reduced glutathione decreased, whereas TBARS and plasma antioxidant capacity was increased) as well as in erythrocyte (increased levels of TBARS and protein carbonyls). Erythrocyte antioxidant enzyme activities were also significantly changed by handball. Muscle damage indices (creatine kinase and lactate dehydrogenase) increased significantly after exercise. In addition, IL-6 increased after the game, whereas TNF-αdecreased during recovery.Conclusion. This study demonstrates that a single handball game in elite athletes induces a marked state of oxidative stress evidenced by the oxidative modification in plasma and erythrocyte macromolecules, as well as by changes in the enzymatic and nonenzymatic antioxidant system.

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NUPR1: A Critical Regulator of the Antioxidant System

Cancers ◽

10.3390/cancers13153670 ◽

2021 ◽

Vol 13 (15) ◽

pp. 3670

Author(s):

Can Huang ◽

Patricia Santofimia-Castaño ◽

Juan Iovanna

Keyword(s):

Oxidative Stress ◽

Antioxidant System ◽

Mitochondrial Function ◽

Therapeutic Potential ◽

Current Knowledge ◽

Redox Homeostasis ◽

Antioxidant Genes ◽

Intrinsically Disordered ◽

And Oxidative Stress

Nuclear protein 1 (NUPR1) is a small intrinsically disordered protein (IDP) activated in response to various types of cellular stress, including endoplasmic reticulum (ER) stress and oxidative stress. Reactive oxygen species (ROS) are mainly produced during mitochondrial oxidative metabolism, and directly impact redox homeostasis and oxidative stress. Ferroptosis is a ROS-dependent programmed cell death driven by an iron-mediated redox reaction. Substantial evidence supports a maintenance role of the stress-inducible protein NUPR1 on cancer cell metabolism that confers chemotherapeutic resistance by upregulating mitochondrial function-associated genes and various antioxidant genes in cancer cells. NUPR1, identified as an antagonist of ferroptosis, plays an important role in redox reactions. This review summarizes the current knowledge on the mechanism behind the observed impact of NUPR1 on mitochondrial function, energy metabolism, iron metabolism, and the antioxidant system. The therapeutic potential of genetic or pharmacological inhibition of NUPR1 in cancer is also discussed. Understanding the role of NUPR1 in the antioxidant system and the mechanisms behind its regulation of ferroptosis may promote the development of more efficacious strategies for cancer therapy.

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Sinomenine Protects PC12 Neuronal Cells against H2O2-induced Cytotoxicity and Oxidative Stress via a ROS-dependent Up-regulation of Endogenous Antioxidant System

Cellular and Molecular Neurobiology ◽

10.1007/s10571-017-0469-1 ◽

2017 ◽

Vol 37 (8) ◽

pp. 1387-1398 ◽

Cited By ~ 7

Author(s):

Hua Fan ◽

Qing Shu ◽

Xinlei Guan ◽

Jiegang Zhao ◽

Junqiang Yan ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant System ◽

Neuronal Cells ◽

Endogenous Antioxidant ◽

And Oxidative Stress

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Changes in antioxidant system and oxidative stress under water stress in four cucumber cultivars

Indian Journal of Plant Physiology ◽

10.1007/s40502-017-0285-0 ◽

2017 ◽

Vol 22 (1) ◽

pp. 114-119

Author(s):

Fariba Amini ◽

Mehri Askary ◽

Mahnaz Haghir ◽

Hamid Reza Ghassemi

Keyword(s):

Oxidative Stress ◽

Water Stress ◽

Antioxidant System ◽

And Oxidative Stress

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Evaluation of biochemical parameters and oxidative stress in native and crossbred cattle naturally infected with Dermatophytosis

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2020.13.2.0357 ◽

2020 ◽

Vol 13 (2) ◽

pp. 099-104

Author(s):

Apaydin Yildirim Betul

Keyword(s):

Oxidative Stress ◽

Infectious Disease ◽

Lipid Peroxidation ◽

Antioxidant System ◽

Biochemical Parameters ◽

Laboratory Animals ◽

Crossbred Cattle ◽

Healthy Cattle ◽

The World ◽

And Oxidative Stress

Dermatophytosis is an endemic superficial zoonotic fungal infectious disease seen in many countries of the world affecting humans, cats, dogs, cattle, laboratory animals such as rabbits. T. verrucosum considered the main cause of ringworm in cattle. Cattle typically have circular, white-gray crust-shaped lesions on the skin on the neck and head. This study was carried out on a total of 40 native and crossbred cattle, 20 clinically healthy cattle and 20 clinical cases of dermatophytosis. The influence of dermatophytosis on lipid peroxidation and the antioxidant system was investigated. Serum MDA, TAS, TOS, GSH, GPx, SOD and CAT level were measured in groups. Cattle in group dermatophytosis had significantly higher MDA and TOS level and TAS, GSH levels, GPx, SOD and CAT activities were significantly lower (P < 0.001). These findings suggest a relationship between dermatophytosis, the oxidant-antioxidant system and lipid peroxidation.

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The effect of exogenous melatonin on behavior and oxidative stress indicators in the brain of aging mice with experimental models of nervous system pathology

INTERNATIONAL NEUROLOGICAL JOURNAL ◽

10.22141/2224-0713.17.2.2021.229893 ◽

2021 ◽

Vol 17 (2) ◽

pp. 37-43

Author(s):

I.F. Labunets ◽

N.A. Utko ◽

T.N. Panteleymonova ◽

G.M. Butenko

Keyword(s):

Oxidative Stress ◽

Nervous System ◽

Antioxidant Properties ◽

Step Length ◽

Experimental Models ◽

Antioxidant Protection ◽

Exogenous Melatonin ◽

Positive Effects ◽

The Brain ◽

And Oxidative Stress

Background. There is a connection between impaired functioning of the nervous system and oxidative stress in Parkinson’s disease and multiple sclerosis. The influence of age on the development of these pathologies was shown, as well as the antioxidant properties of the hormone melatonin. The purpose was to investigate the effect of melatonin administration on the behavior, factors of oxidative stress and antioxidant protection in the brain of aging mice with experimental models of parkinsonism and demyelination. Materials and methods. 129/Sv mice aged 15–16 months received neurotoxins 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at a dose of 30 mg/kg once, or cuprizone daily with food for 3 weeks. Melatonin was administered at a dose of 1 mg/kg daily at 6 p.m. starting from day 7–8 of toxin exposure. The content of malondialdehyde, the activity of antioxidant enzymes in the brain and behavior parameters were assessed in the open field tests for rigidity and in the rotarod test. Results. The locomotor, emotional and exploratory activities in mice with parkinsonism and demyelination models are lower than those in intact animals. Muscle tone decreases under the influence of cuprizone and increases after MPTP injection; the step length decreases in parkinsonism. Melatonin treatment resulted in increasing the number of squares, step length, and decreasing the retention time on a rotating cylinder in mice with parkinsonism and increasing the number of squares, rea-ring and number of boluses in cuprizone-treated mice. Exogenous melatonin reduces the level of brain malondialdehyde increased by neurotoxins and increases the reduced activity of superoxide dismutase and catalase in mice with parkinsonism, catalase and glutathione peroxidase in mice with demyelination. Conclusions. The positive effects of melatonin on the behavior of aging mice with the MPTP parkinsonism model and the cuprizone model of demyeli-nation are mediated by increased antioxidant protection in the brain.

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Soil applied glycine betaine with Arbuscular mycorrhizal fungi reduces chromium uptake and ameliorates chromium toxicity by suppressing the oxidative stress in three genetically different Sorghum (Sorghum bicolor L.) cultivars

BMC Plant Biology ◽

10.1186/s12870-021-03113-3 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Praveen Kumar

Keyword(s):

Oxidative Stress ◽

Grain Yield ◽

Glycine Betaine ◽

Block Design ◽

Growth Stages ◽

Chromium Toxicity ◽

Yield Attributes ◽

Cr Toxicity ◽

Chromium Uptake ◽

Cr Uptake

Abstract Background Chromium is the most toxic pollutant that negatively affects a plant’s metabolic activities and yield. It reduces plant growth by influencing the antioxidant defence system’s activities. In the present study, a completely randomized block design experiment with three plants/pot in three replication was conducted on three varieties of sorghum viz. SSG 59–3, HJ 513 (multi-cut) and HJ 541 (single-cut) for amelioration of chromium toxicity (2 & 4 ppm) by exogenous application of GB (50 & 100 mM) with and without AMF in soil. The ameliorative effects were tested at two growth stages viz. vegetative (35 DAS) and grain filling (95 DAS), in terms of Cr uptake, grain yield, antioxidative defence system parameters (viz. enzymes – SOD, APX, CAT, GR, POX and metabolites – proline, glutathione, ascorbate, β-carotene) and indices of oxidative stress parameters (viz. PPO, H2O2, and MDA). Results The results delineated that Cr uptake and indices of oxidative stress were increased with increasing concentration of Cr stress in all the varieties (HJ 541, HJ513 & SSG 59–3) at both the growth stages (35 & 95 DAS). At higher concentration (4 ppm), Cr stress decreased the grain yield (45–50%) as compared with controls. Polyphenoloxidase activity, MDA and H2O2 content increased at both growth stages in all the varieties. However, antioxidative enzymes and metabolite activities increased due to Cr stress but this increase was not sufficient to counteract with ROS generated under Cr stress which was enhanced on the application of AMF and GB either individually or in combination (spiked in soil). It decreased the indices of oxidative stress and ameliorated the Cr toxicity and increased grain yield (65–70%) in all the varieties. Conclusions Both GB and AMF improved the antioxidative activities and stress tolerance capacity of the plant. Glycine betaine at both 50 and 100 mM level, significantly ameliorated Cr toxicity. However, AMF concomitantly with GB further boosts up the amelioration behaviour of the plant against Cr toxicity, at both growth stages in all the varieties. The combination of 100 mM GB with 10 g AMF was observed most effective among all the treatments. Among the varieties, SSG 59–3 had the lowest chromium uptake, indices of oxidative stress, and highest antioxidative system’s activity as compared to HJ 513 followed by HJ 541 variety. Thus AMF and GB either individually or in combination may be used to maintain plant yield attributes under Cr toxicity.

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