The impact of low mineral content water on cardiac function in diabetic rats: focus on oxidative stress

2020 ◽  
Vol 472 (1-2) ◽  
pp. 135-144
Author(s):  
Dijana Lalovic ◽  
Vladimir Jakovljevic ◽  
Kristina Radoman ◽  
Jovana Bradic ◽  
Nevena Jeremic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cardiac Function ◽  
Mineral Content ◽  
Diabetic Rats ◽  
The Impact

scholarly journals The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1332
Author(s):  
Gilda M. Iova ◽  
Horia Calniceanu ◽  
Adelina Popa ◽  
Camelia A. Szuhanek ◽  
Olivia Marcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Rats ◽  
Gingival Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
The Impact ◽  
Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

scholarly journals Protective effect of crocin and voluntary exercise against oxidative stress in the heart of high-fat diet-induced type 2 diabetic rats

2016 ◽  
Vol 103 (4) ◽  
pp. 459-468 ◽  
Author(s):  
V Ghorbanzadeh ◽  
M Mohammadi ◽  
G Mohaddes ◽  
H Dariushnejad ◽  
L Chodari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
High Fat Diet ◽  
Critical Role ◽  
Diabetic Rats ◽  
Voluntary Exercise ◽  
High Fat ◽  
The Impact ◽  
Type 2 Diabetic

Background Oxidative stress plays a critical role in the pathogenesis and progression of type 2 diabetes and diabetic-associated cardiovascular complications. This study investigated the impact of crocin combined with voluntary exercise on heart oxidative stress indicator in high-fat diet-induced type 2 diabetic rats. Materials and methods Rats were divided into four groups: diabetes, diabetic-crocin, diabetic-voluntary exercise, diabetic-crocin-voluntary exercise. Type 2 diabetes was induced by high-fat diet (4 weeks) and injection of streptozotocin (intraperitoneally, 35 mg/kg). Animals received crocin orally (50 mg/kg); voluntary exercise was performed alone or combined with crocin treatment for 8 weeks. Finally, malondialdehyde (MDA), activity of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were measured spectrophotometrically. Results Treatment of diabetic rats with crocin and exercise significantly decreased the levels of MDA (p < 0.001) and increased the activity of SOD, GPx, and CAT compared with the untreated diabetic group. In addition, combination of exercise and crocin amplified their effect on antioxidant levels in the heart tissue of type 2 diabetic rats. Conclusion We suggest that a combination of crocin with voluntary exercise treatment may cause more beneficial effects in antioxidant defense system of heart tissues than the use of crocin or voluntary exercise alone.

scholarly journals PO-190 Exercise Training Protects Against Cardiac Pathological Remodeling in Myocardial Infarction rats via Improving Mitochondrial Biogenesis

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Dandan Jia ◽  
Zhenjun Tian
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Exercise Training ◽  
Cardiac Function ◽  
Myocardial Fibrosis ◽  
Mitochondrial Biogenesis ◽  
Cardiac Dysfunction ◽  
Myocardial Apoptosis ◽  
The Impact ◽  
Pathological Remodeling

Objective  Growing evidence suggests that exercise training reverses cardiac pathological remodeling and cardiac dysfunction during myocardial infarction (MI), but the underlying mechanisms have not been fully understood. In this study, we investigated the impact of exercise training on cardiac function, myocardial fibrosis, apoptosis, oxidative stress and mitochondrial biogenesis. Methods Sprague Dawley rats were subjected to MI by permanent ligation of the left anterior descending (LAD) coronary artery or Sham operation. Rats with MI were randomly assigned to sedentary MI group (MI) and MI with exercise training group (MI+EX), and compared to sham-operated group (Sham). Haemodynamics and Masson staining were conducted to evaluate the effect of exercise training on cardiac function and myocardial fibrosis. Myocardial apoptosis, oxidative stress, mitochondrial biogenesis and molecular signaling mechanism were analyzed. Results  Exercise training significantly improves cardiac function and mitigates the MI-induced cardiac pathological remodeling. Meanwhile, Exercise training significantly attenuates MI-induced apoptosis, oxidative stress and mitochondrial biogenesis. In addition, activation of PI3K pathway following MI is further induced by exercise training. Conclusions  Exercise training protects against MI-induced cardiac dysfunction and pathological remodeling through preventing myocardial apoptosis and oxidative stress, and enhancing mitochondrial biogenesis.

scholarly journals The potential therapeutic effects of the gut microbiome manipulation by synbiotic containing-Lactobacillus plantarum on neuropsychological performance of diabetic rats

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Mohammad Morshedi ◽  
Maryam Saghafi-Asl ◽  
Elaheh-Sadat Hosseinifard
Keyword(s):  
Oxidative Stress ◽  
Gut Microbiota ◽  
Gut Microbiome ◽  
Therapeutic Potential ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Microbial Composition ◽  
Stress Markers ◽  
Oxidative Stress Status ◽  
The Impact

Abstract Background The manipulation of gut microbiota as a target has been suggested to reduce the risks for a number of diseases such as type 2 diabetes mellitus (T2DM). Conversely, T2DM is associated with complications such as gut and brain disorders. Furthermore, the impact of probiotics and prebiotics to improve T2DM complications are reported. Thus, the present study seeks to investigate the therapeutic and neuropsychological effects of L. plantarum and inulin in diabetic rats. Methods Throughout the investigation, L. plantarum, inulin or their combination (synbiotic) was administered to diabetic rats. in the end, fecal samples were collected to evaluate the gut microbial composition. Then behavioral tests were conducted. Subsequently, the obtainment of the prefrontal cortex (PFC) and hippocampal samples. Results Our data demonstrated that administration of L. plantarum and inulin could improve gut dysbiosis and oxidative stress status. In addition, it could ameliorate serotonin and BDNF/TrkB signaling pathway. Notably, a strong correlation between the gut microbiota changes and cognition responses was observed. Interestingly, synbiotics intake exploited a rather powerful effect on oxidative stress markers. Conclusion The findings confirm that there is a beneficial therapeutic potential of supplements, especially symbiotic. Moreover, neuropsychological improvement associated with balanced gut microbiome.

Impact of alcohol on hepatic mitochondrial DNA damage in streptozotocin diabetic rats

2017 ◽  
Vol 2 (2) ◽  
pp. 90 ◽  
Author(s):  
Swarnalatha Kodidela ◽  
Suresh Govatati ◽  
Sumanth Kumar Matha ◽  
Swapna Vahini Korla ◽  
B. Prathap Naidu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Mitochondrial Dna ◽  
Diabetic Rats ◽  
Sequencing Analysis ◽  
Enzymatic Antioxidants ◽  
Mitochondrial Dna Damage ◽  
Mtdna Deletions ◽  
D Loop ◽  
The Impact

Mitochondrial dysfunction with increased production of reactive oxygen species (ROS) is a characteristic feature of diabetes which is associated with damage of mitochondrial DNA (mtDNA). Alcohol metabolism generates ROS with enhanced oxidative stress leading to damage of cellular constituents including mtDNA. The aim of the present study is to investigate the impact of alcohol consumption on hepatic mtDNA damage in diabetic rats. MtDNA was isolated from hepatic tissues of non-diabetic and streptozotocin induced diabetic rats after alcohol treatment. Comprehensive screening of mtDNA displacement loop (D-loop) was carried out by PCR-Sanger’s sequencing analysis. MtDNA deletions were analyzed by long-extension PCR. Furthermore, activities of enzymatic and non-enzymatic antioxidants were measured in hepatic tissue of all rats. Our results showed increased frequency of D-loop mutations in alcoholic-diabetic rats when compared to diabetic or alcoholic non-diabetic rats. DNA mfold analysis predicted higher free energy for 15507C, 15560T and 16116C alleles compared to their corresponding wild alleles which represents less stable secondary structures with negative impact on overall mtDNA function. MtDNA deletions were observed in all experimental groups except controls. Markedly decreased activities of antioxidant enzymes viz., GPx, SOD, catalase and GSH content was identified in alcoholic-diabetic rats when compared to remaining groups. In conclusion, decreased GSH content and lowered activity of catalase, SOD and GPx favor the environment for oxidative stress, which might lead to exacerbation of hepatic mitochondrial DNA damage in diabetic rats receiving alcohol.

Abstract 1473: Cardiac Targeted Transgenic Models of Mutations in TK2 or Pol γ Genes Result in Ultrastructural Changes in Mitochondria and Cardiac Hypertrophy

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
James J Kohler ◽  
Seyed Hosseini ◽  
Brian Day ◽  
Ann Saada ◽  
Orly Elpeleg ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cardiac Hypertrophy ◽  
Cardiac Function ◽  
Point Mutations ◽  
Ultrastructural Changes ◽  
Mitochondrial Oxidative Stress ◽  
Mtdna Depletion ◽  
Lv Mass ◽  
Mtdna Replication ◽  
The Impact

Background: Mitochondrial (mt) biogenesis and homeostasis of precursor pools are critical to normal cardiac performance. Two important enzymes are involved in mtDNA replication. DNA polymerase gamma (Pol γ) is responsible for replication of mtDNA. Thymidine kinase 2 (TK2) is responsible for intramitochondrial monophosphorylation of pyrimidines. Point mutations in Pol γ and TK2 identified in mitochondrial genetic diseases include human chronic progressive external opthalmoplegia (CPEO) associated with a Pol γ Y955C mutation, while His121Asn (HIS) and Ile212Asn (ILE) mutations in TK2 result in heritable mtDNA depletion syndromes with organ dysfunction. Because nucleoside reverse transcriptase inhibitors (NRTI) can also disrupt mtDNA replication, we examined the impact of cardiac-targeted transgenic overexpressors of Pol γ or TK2 mutants in combination with NRTI-based HAART on cardiac function. Methods: Targeted transgenic mice (TG) were generated that overexpress Y955C, HIS or ILE in cardiac tissues driven by the α-MyHC promoter. Changes in cardiac and mitochondrial structure and function were examined by echocardiography (ECHO), transmission electron microscopy (EM), mtDNA abundance, 8-OH2dG and SDH enzyme histochemistry quantitation. TGs and wild-type (WT) littermates were treated with vehicle or NRTI-based HAART (ziduvodine, lamivudine, and indinavir, 35 days). Results: Y955C TGs had decreased cardiac mtDNA abundance, increased mitochondrial oxidative stress (increased 8-OH2dG), and mitochondrial destruction together with increased LV mass. HIS TGs (both treated and untreated) demonstrated increased LV mass and a 2-fold increase in mtDNA abundance compared to WT. Untreated ILE TGs had no analogous impact, but the addition of HAART treatment resulted in increased LV mass and mtDNA abundance. Conclusions: Inefficient mtDNA replication and mitochondrial oxidative stress contribute to mtDNA depletion. Pol γ Y955C and TK2 HIS each alters mtDNA replication. ILE had minimal impact on mitochondrial ultrastructure or cardiac function, but together with HAART had an effect. These pathogenic point mutations in genes involved in mtDNA replication may increase risk for cardiac hypertrophy compounded by NRTI treatment.

scholarly journals The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats

2019 ◽  
Author(s):  
Seddigheh Sheikh Hosseini ◽  
Ali Gol ◽  
Moje Khaleghi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Glutathione Peroxidase ◽  
Normal Saline ◽  
Lactobacillus Acidophilus ◽  
Diabetic Rats ◽  
Male Rats ◽  
Significant Difference ◽  
Anti Oxidant ◽  
The Impact

Background: Oxidative stress plays an important role in the development of diabetic complications. Objective: This study evaluated the impact of pre- and post-treatment with Lactobacillus acidophilus ATCC 4356 on the oxidant and anti-oxidant factors of testis and epididymis in streptozotocin-induced diabetic rats. Materials and Methods: Thirty male Wistar rats (10 wk old) weighing 220-230 g. were divided into five groups (n = 6/ each): 1- normal group, 2- normal lactobacillus group, 3- diabetic group, 4- diabetic + lactobacillus before (DLB) group, and 5- diabetic + lactobacillus after (DLA) group. The normal and diabetic groups received daily 1 mL normal saline for 6 wk. Normal lactobacillus group received daily L. acidophilus for 6 wk. Group DLB received daily L. acidophilus for 2 wk before diabetes and for 4 wk after diabetes. Group DLA received daily 1 mL normal saline for 2 wk before diabetes and L. acidophilus for 4 wk after diabetes. The dose of L. acidophilus was 1 × 109 CFU/mL. Results: The administration of L. acidophilus worsened blood glucose level and reduced the levels of Malondialdehyde (p = ≤ 0.0001) and Hydrogen peroxide (p ≤ 0.0001) and, Catalase and Glutathione peroxidase activity increased in the testis. In epididymis, Glutathione peroxidase and Catalase (p = 0.013) activity increased and Hydrogen peroxide concentration reduced, while Malondialdehyde concentration did not show any changes compared to the diabetic rats. Also, there was no significant difference between DLB and DLA groups, in these markers. Conclusion: Data obtained suggests that L. acidophilus has anti-oxidant effects on the testis and sometime in the epididymis in diabetic rats.

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