scholarly journals Mycotoxins occurrence in medicinal herbs dietary supplements and exposure assessment

2021 ◽  
Author(s):  
Noelia Pallarés ◽  
Houda Berrada ◽  
Guillermina Font ◽  
Emilia Ferrer
Keyword(s):  
Dietary Supplements ◽  
Ion Trap ◽  
Medicinal Herbs ◽  
Risk Characterization ◽  
Quechers Method ◽  
European Pharmacopoeia ◽  
Deterministic Method ◽  
The Mean ◽  
Daily Intakes ◽  
Herbal Ingredient

AbstractThe multimycotoxin analysis of aflatoxins (AFs), zearalenone (ZEA), ochratoxin A (OTA), enniatins (ENNs) and beauvericin (BEA) was performed in 85 samples of medicinal herbs dietary supplements. The samples were classified in 64 samples of one herbal ingredient and 21 mixed samples. The extraction was performed by QuEChERS method and the determination by liquid chromatography coupled to ion-trap tandem mass spectrometry (LC–MS/MS-IT). Then, the risk characterization to mycotoxins through the consumption of medicinal herbs dietary supplements was assessed. The results showed that ZEA, OTA, ENNs and BEA showed in the samples with incidences between 1 and 34%, being ENNB the most detected mycotoxin. Mycotoxins contents ranged from LOQ to 3850.5 µg/kg while the mean of positives samples were 65.5 µg/kg (ENNA), 82.7 µg/kg (ENNA1), 88.7 µg/kg (ENNB), 324.9 µg/kg (ENNB1), 137.9 µg/kg (BEA) and 1340.11 µg/kg (ZEA), respectively. OTA was detected in one herbal mix tablet for insomnia at concentration of 799 μg/kg. In herbal drugs the European Pharmacopoeia Commission has implemented limits of 2 µg/kg for AFB1 and 4 µg/kg for total AFs. In the present study AFs have not been detected in the analyzed medicinal herbs dietary supplements. The Estimated Daily Intakes (EDIs) values were calculated using a deterministic method, considering two exposure scenarios (lower bound (LB) and upper bound (UB)). The values obtained were in general far below the Tolerable Daily Intakes (TDIs) established. Graphical abstract

The effect of antibiotic dietary supplements on the carcass measurements and dressing percentage of bacon pigs

1955 ◽  
Vol 46 (2) ◽  
pp. 173-179 ◽  
Author(s):  
G. Harrington ◽  
J. H. Taylor
Keyword(s):  
Growth Rate ◽  
Vitamin B12 ◽  
Dietary Supplements ◽  
Vegetable Protein ◽  
Animal Protein ◽  
Feeding Trials ◽  
The Mean ◽  
Dressing Percentage ◽  
And Control

1. Certain carcass measurements (length, shoulder and mid-back fats, belly thickness and dressing percentage) made on a total of 402 bacon pigs from four antibiotic feeding trials have been analysed.2. The length of the carcasses showed more variation between pens treated with various doses of penicillin than between control pens or pens treated with aureomycin. This greater variation in length was related to the greater variation in growth rate between the penicillin treatments. No differences in the mean lengths of control, penicillin and aureomycin treatments were found.3. Back fat measurements and belly thickness showed no differences between aureomycin, penicillin and control treatments in overall tests.4. Aureomycin consistently gave higher dressing percentages than penicillin. Animal protein also gave higher dressing percentages than vegetable protein, but no effect of vitamin B12 on this quantity was found.

scholarly journals Relationships between flavonoids and selected elements in infusions of medicinal herbs

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Pawel Konieczynski ◽  
Agnieszka Arceusz ◽  
Marek Wesolowski
Keyword(s):  
Principal Component Analysis ◽  
Principal Component ◽  
Total Content ◽  
Essential Elements ◽  
Medicinal Herbs ◽  
Total Flavonoids ◽  
Aqueous Extracts ◽  
Zn And Cu In ◽  
Total Flavonoids Content ◽  
The Mean

AbstractThe aim of the studies was to establish relationships between flavonoids and elements important for human health. Therefore, total contents of flavonoids and phosphorus were determined by UV/Vis methods, flavonoids by HPLC, and Ca, Mg, Fe, Mn, Zn and Cu by FAAS in 68 infusions of medicinal herbs. Total flavonoids content in the aqueous extracts were in the range of 0.26 - 16.40 mg per 100 mL. The mean flavonoid contents (in mg per 100 mL of aqueous extract) were 2.24, 2.01, 1.83, 1.88 for rutin, myricetin, quercetin and kaempferol, respectively. The concentrations of Ca, Mg, P were determined in mg per 100 mL, and of Fe, Mn, Zn and Cu in μg per 100 mL. Total content of flavonoids was weakly correlated with quercetin (r = 0.41), kaempferol (r = 0.53), Cu (r = 0.43), and Ca (r = -0.30). Statistically significant correlations were also found among Cu, Ca, Mn, Zn and Fe. Cluster analysis grouped the studied herbs based on total flavonoids, also four flavonoids and essential elements contents, extracted from the whole population of herbs Sambuci flos, Betulae folium, and Sylibi mariani semen. Principal component analysis confirmed these findings and enabled identification of quercetin, kaempferol, Cu and Fe as the factors responsible for differentiation of the studied material.

scholarly journals Development and Validation of an UHPLC Method for Ostarine Determination in Dietary Supplements

2019 ◽  
Vol 65 (2) ◽  
pp. 49-54
Author(s):  
Amalia Miklos ◽  
Amelia Tero-Vescan ◽  
Lénárd Farczádi ◽  
Daniela-Lucia Muntean
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Low Cost ◽  
Reversed Phase ◽  
European Pharmacopoeia ◽  
Average Mass ◽  
7Th Edition ◽  
Development And Validation ◽  
Mg Content

AbstractObjective: The purpose of this study was to develop a low-cost, yet sensitive and precise UHPLC method for the quantitative determination of ostarine from dietary supplements (DS) for athletes. The analytical performance of the method was verified on a DS legally acquired from a specialized website for athletes. The uniformity of mass and content of the ostarine DS was also verified.Methods: For the quantitative determination of ostarine a UHPLC method was developed and validated. The separation was performed using a reversed-phase C18 column, using a mixture of 75% methanol: 25% formic acid 0.1% in isocratic elution, at a flow rate of 0.5 ml/min. The uniformity of mass and content of DS was performed following the methodology described in the European Pharmacopoeia 7th Edition.Results: The validated method was specific and linear on the concentration range of 1-25 µg/ml and was precise and accurate at all concentration levels, according to the official guidelines for validating analytical methods. An average mass of 510 mg content was obtained for the ostarine capsules, with an RSD of 2.41%. Regarding the uniformity of the content, an average of 4.65 mg ostarine/capsule was obtained with an RSD of 1.05%.Conclusions: The developed UHPLC method was suitable, rapid, sensitive and allowed quantitative determination of active substance content in a DS with ostarine (92.91% ostarine/capsule from 5 mg ostarine/capsule declared by the manufacturer).

scholarly journals The effect of artificial sweeteners on body weight of mice

2021 ◽  
Vol 93 (2) ◽  
Author(s):  
Iyad Ali ◽  
Naser Shraim ◽  
Wafaa’ Atrash ◽  
Aisha Sirafi ◽  
Huda Abadi
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Fluid Intake ◽  
Weight Maintenance ◽  
Zingiber Officinale ◽  
The Body ◽  
Thymus Vulgaris ◽  
Artificial Sweeteners ◽  
The Mean ◽  
Daily Intakes

Artificial Sweeteners (AS) are synthetic sugar substitutes that have sweetening potency hundreds of times more than the table sugar (sucrose). Artificial sweeteners are regarded as attractive alternatives to sugar as they add no calories to food intake. There are many hypotheses suggesting that AS may enhance appetite and cause weight gain. The aim of this study was to evaluate the effect of AS on food intake, fluid intake and body weight of mice. Acceptable daily intakes of AS solutions were administered orally to different set of mice for four weeks. The body weight, food consumption and fluid intake were measured. At the same time, the effect of Zingiber officinale extracts (natural appetite suppressor), Thymus vulgaris extracts (natural appetite inducer) and cyproheptadine (an appetite stimulant drug) on body weight of mice was evaluated. Artificial sweeteners consumption cause insignificant changes in body weight (p>0.05). However, the mean consumption of food and solutions varies significantly for some groups. The consumption of AS has no significant effect on body weight and may contribute to weight maintenance and energy balance as substitutes to high caloric sugar

Determination of mean daily intakes of aflatoxin B1, aflatoxin M1, ochratoxin A, trichothecenes and fumonisins in 24-hour diets of children in the Netherlands

2009 ◽  
Vol 2 (4) ◽  
pp. 451-459 ◽  
Author(s):  
G. Bakker ◽  
E. Sizoo ◽  
A. Jekel ◽  
D.P. Pereboom-de Fauw ◽  
R. Schothorst ◽  
...  
Keyword(s):  
The Netherlands ◽  
Ochratoxin A ◽  
Daily Intake ◽  
Aflatoxin M1 ◽  
Limit Of Quantification ◽  
Diet Study ◽  
The Mean ◽  
Daily Intakes ◽  
Aflatoxin B

In 2006, a duplicate diet study of children's food was carried out in the Netherlands. Parents or guardians of 123 children collected duplicates of the 24-hour diets. Levels of aflatoxin M1, aflatoxin B1, ochratoxin A, trichothecenes and fumonisins were determined. Aflatoxin M1 was detectable in 10% of the samples, with all toxin levels below the limit of quantification. Aflatoxin B1 could be detected in 80% of the samples, while in 47% of all samples aflatoxin B1 was quantifiable. Ochratoxin A could be quantified in all samples. Deoxynivalenol was quantified in almost every sample, while T-2 and HT-2 toxins could only be quantified in 3.2% and 6.4% of the samples respectively. 15-acetyldeoxynivalenol was detected in 1.6% of the samples. Fumonisin B1 was detected in 28% of the samples and fumonisin B2 in a quarter of merely those samples where fumonisin B1 was detected. In 20% of the samples fumonisin B1 could be quantified and in a quarter of those samples fumonisin B2 could be quantified too. The analytical results were used to estimate levels of daily intake. Only the mean daily intake levels for aflatoxin B1, ochratoxin A, deoxynivalenol and fumonisins B1 and B2 could reliably be estimated. The values were 0.1, 4.1, 291 and 28 ng/kg bw/day respectively, all are well below the corresponding tolerable daily intakes. For aflatoxin B1 a tolerable intake does not exist, but the intake value for this mycotoxin was very low if compared to the value that would result from the intake of food, if it was contaminated with aflatoxin B1 at the EU regulatory limit, specified for baby food. The mean daily intakes of the mycotoxins determined in children's food in the Netherlands are low and implicate that there is no health risk for children due to exposure from the studied mycotoxins.

A multi-centre collaborative study on the potency estimation of ReFacto

2003 ◽  
Vol 90 (12) ◽  
pp. 1088-1093 ◽  
Author(s):  
Dawn Sands ◽  
Eva Sandberg ◽  
Rainer Seitz ◽  
Trevor Barrowcliffe ◽  
Anthony Hubbard
Keyword(s):  
Factor Viii ◽  
Collaborative Study ◽  
Full Length ◽  
European Pharmacopoeia ◽  
International Standard ◽  
Assigned Value ◽  
Coagulant Activity ◽  
The Mean ◽  
Good Agreement

SummarySeven laboratories estimated factor VIII coagulant activity in recombinant B-domain-deleted (ReFacto) and plasma-derived FVIII concentrates (Octonativ-M) using chromogenic methods relative to the WHO 6th International Standard FVIII Concentrate (WHO 6th IS), European Pharmacopoeia BRP#2 (EP#2) and the ReFacto Laboratory Standard (RLS). Significantly higher estimates were obtained for all batches of product when calculated relative to the RLS in comparison with estimates vs WHO 6th IS and EP#2. Mean estimates for two batches of ReFacto product vs the RLS were within 10% of the labelled potency whereas estimates vs WHO 6th IS and EP#2 ranged from 21 to 31% lower than the label. Conversely, mean estimates for Octonativ-M relative to WHO 6th IS and EP#2 were within 10% of the label whereas the mean estimate vs RLS was 117% of label. Mean estimates for the ReFacto product, vs the WHO 6th IS and EP#2, varied considerably between the different chromogenic kits whereas estimates vs the RLS showed good agreement between kits. Mean estimates for the RLS vs the WHO 6th IS (8.10 IU/vial) and the EP#2 (7.66 IU/vial) were lower than the assigned value of 9.4 IU/vial. The results are consistent with ReFacto and full-length FVIII responding differently to variations in assay methodology and also indicate that the assigned value on the RLS may be too high. Since this study the unitage on the RLS has been adjusted to effectively increase the amount of ReFacto in the product by 20%.

scholarly journals Copper, iron, manganese and zinc concentrations in the carcases of lambs and calves and the relationship to trace element requirements for growth

1979 ◽  
Vol 42 (1) ◽  
pp. 89-96 ◽  
Author(s):  
N. F. Suttle
Keyword(s):  
Dry Matter ◽  
Live Weight ◽  
Tissue Storage ◽  
Growth Requirements ◽  
Fe And Mn ◽  
Zinc Concentrations ◽  
The Mean ◽  
Daily Intakes ◽  
Iron Manganese ◽  
The Relationship

1. The minced carcases of twenty-seven lambs, ranging from 18 to 69 kg in live weight, and twenty-five calves (30–90 kg) were analysed for copper, iron, manganese and zinc. The lambs were weaned whereas the calves were reared exclusively on milk.2. Mean concentrations of Fe, Mn and Zn for groups of lamb carcases fell within the ranges 52.6–75.1, 0.7–1.2 and 20.8–25.6 mg/kg fresh carcase weight respectively. The concentrations of Fe and Mn decreased while that of Zn increased slightly with age at slaughter. The concentrations of Fe, Mn and Zn in calves were close to thosein lambs.3. For both species, the concentration of Cu in the carcase varied erratically: variation in hepatic Cu storage was implicated. In an additional study of ten full-term foetuses from Cu-depleted or Cu-supplemented ewes, a dietary Cu supplement (10 mg/kg dry matter (DM)) increased foetal Cu status 10-fold, due largely to an increase in foetal liver Cu.4. The mean retentions of trace elements in the lamb carcases (%intake) were approximately: Cu 2.0, Fe 1.3, Mn 0.08, Zn 4.0. The corresponding values for the milk-fed calves were all probably much higher (Cu 23, Fe 43.7, Mn 4.9, Zn 34.0) but Cu intake was not accurately measured.5. After allowing for tissue storage of Fe and Mn, values of 55, 0.85 and 24 mg/kg carcase gain were taken to represent the approximate net growth requirements of lambs for Fe, Mn and Zn respectively: the corresponding value for Cu was probably < 1.0 mg/kg. Values for calves were similar to those for lambs.6. It was concluded that the total net requirements of ruminants for Fe and Zn shouldbe considered in terms of daily intakes of the metans rather than dietary concentrationsbecause of the relatively large and constant contribution of the growth component to thetotal requirement.

The voluntary intake by cattle of four silages differing in dry matter content

1970 ◽  
Vol 12 (4) ◽  
pp. 591-599 ◽  
Author(s):  
N. Jackson ◽  
T. J. Forbes
Keyword(s):  
Dry Matter ◽  
Live Weight ◽  
Matter Content ◽  
Metabolizable Energy ◽  
Dry Matter Content ◽  
Meadow Fescue ◽  
Digestible Organic Matter ◽  
The Mean ◽  
Voluntary Intake ◽  
Daily Intakes

SUMMARYHerbage from the same timothy/meadow fescue/white clover sward was ensiled at four different dry matter contents. The resulting silages had dry matter contents of 19·0, 27·3, 32·3 and 43·2%, the dry matter content increasing with the length of the wilting period. An experiment was carried out to determine the voluntary intake of the silages. Each silage was given to 7 animals individually, the mean live weight of these being 334 kg.Although the silages made from wilted herbage were lower in digestibility than that made from unwilted herbage, wilting increased dry matter intake and metabolizable energy (ME) intake. The mean daily intakes of digestible organic matter were 53·0, 58·1, 59·6 and 59·6 g/kgW0·73, for silages of increasing dry matter content. The corresponding ME intakes, expressed as a multiple of the ME requirement for maintenance, were 1·17, 1·29, 1·30 and 1·28. The percentage of acetic acid in the silage dry matter was significantly (r= −0·56) and linearly related to voluntary intake. The relationship between lactic acid concentration and voluntary intake was significantly curvilinear (r= 0·48).

scholarly journals Bioavailability of heavy metals in the soil from different locations of medicinal herbs

2014 ◽  
Vol 31 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Biljana Kaličanin ◽  
Dragan Velimirović ◽  
Ivana Arsić ◽  
Sofija Đorđević
Keyword(s):  
Rural Areas ◽  
Toxic Metal ◽  
Total Content ◽  
Mean Value ◽  
Medicinal Herbs ◽  
Microanalytical Technique ◽  
Highly Sensitive ◽  
Potentiometric Stripping Analysis ◽  
The Mean ◽  
Plant Growing Season

SUMMARY Medicinal herbs and their mixtures, which are widely used for prevention and treatment of some disease, can also present health risks due to the presence of toxic metals such as Pb and Cd. The application of different agrotechnical practices during plant growing season, as well as the process of circulation of substances in nature, may be the cause of plant contamination. The aim of this study was to determine the content of lead, cadmium and copper from soil which are bioavailable for absorption by plant, as well as the total content of these metals in some medicinal herbs that were cultivated in two different locations. The presence of these metals in the samples was detected using highly sensitive microanalytical technique potentiometric stripping analysis (PSA). The mean value of lead total content in the analyzed samples of medicinal herbs Chamimillae flos, Urticae folium, Menthae folium, Altheae radix and Basilici herba, which were grown at the sites near the industrial zone, was about 1.55 μg/g, 1.82 μg/g, 1.90 μg/g, 1.99 μg/g and 2.74 μg/g, respectively. Contrary to this, the total content of this toxic metal in the analyzed plant samples grown on rural areas was detected only on some sites. Based on the results of this study, it can be concluded that medicinal herbs contained a certain amount of lead and that its content varied depending on the location at which the plant species were grown as well as on plant affinity to certain metal. Cadmium and copper were not detected in the tested plant material.

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