Design, characterization and in vitro evaluation of thin films enriched by tannic acid complexed by Fe(III) ions

AbstractMaterials based on carbohydrate polymers may be used for biomedical application. However, materials based on natural polymers have weak physicochemical properties. Thereby, there is a challenge to improve their properties without initiation of toxicity. The alternative method compared to toxic chemical agents’ addition is the use of metal complexation method. In this study, chitosan/tannic acid mixtures modified by Fe(III) complexation are proposed and tested for potential applications as wound dressings. Thereby, surface properties, blood compatibility as well as platelet adhesion was tested. In addition, the periodontal ligament stromal cells compatibility studies were carried out. The results showed that the iron(III) addition to chitosan/tannic acid mixture improves properties due to a decrease in the surface free energy and exhibited a reduction in the hemolysis rate (below 5%). Moreover, cells cultured on the surface of films with Fe(III) showed higher metabolic activity. The current findings allow for the medical application of the proposed materials as wound dressings.

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The characterization of thin films based on chitosan and tannic acid mixture for potential applications as wound dressings

Polymer Testing ◽

10.1016/j.polymertesting.2019.106007 ◽

2019 ◽

Vol 78 ◽

pp. 106007 ◽

Cited By ~ 6

Author(s):

B. Kaczmarek ◽

K. Nadolna ◽

A. Owczarek ◽

M. Michalska-Sionkowska ◽

A. Sionkowska

Keyword(s):

Thin Films ◽

Tannic Acid ◽

Wound Dressings ◽

Acid Mixture ◽

Potential Applications

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A Review of Water-Resistant Cellulose-Based Materials in Pharmaceutical and Biomedical Application

Current Medicinal Chemistry ◽

10.2174/0929867328666210208113354 ◽

2021 ◽

Vol 28 ◽

Author(s):

Bei He ◽

Xinxin Liu ◽

Shi Qi ◽

Run Zheng ◽

Minmin Chang ◽

...

Keyword(s):

Mechanical Properties ◽

Biomedical Application ◽

Drug Loading ◽

Cellulose Fibers ◽

Dip Coating ◽

Wound Dressings ◽

Attractive Alternative ◽

Natural Polymers ◽

Insoluble Drugs ◽

Good Biocompatibility

Background: Cellulose, huge reserves of natural polymers, have been widely applied in pharmaceutical and biomedicine fields due to its good biocompatibility, biodegradability, non-toxicity and excellent mechanical properties. At present, water-resistant metal-based and petroleum-based materials applied in medical field exists obvious problems of poor biocompatibility and high cost. Therefore, water-resistant cellulose-based materials with good biocompatibility and low price will become an attractive alternative. This review aims to summarize the preparation of water-resistant cellulose-based materials and their potential application in pharmaceutical and biomedical in recent years. Methods: Common hydrophobic treatments of cellulose fibers or paper were overviewed. The preparation, properties and applications of water-resistant cellulose-based materials in the pharmaceutical and biomedical fields were summarized. Results: Common hydrophobic treatments of cellulose fibers or paper were divided into chemical modification (graft polymerization, crosslinking, solution casting or dip-coating), physico-chemical surface modifications (plasma treatments, surface patterning, electrostatic spraying and electrowetting) and physical processing (electrostatic spinning, SAS process and 3D EHD printing). These hydrophobically processed cellulose fibers or paper could be prepared into various water-resistant cellulose-based materials and applied in pharmaceutical excipients, drug-loaded amphiphilic micelles, drug-loaded composite fibers, hydrophobic biocomposite film/coatings and paper-based detectors. They presented excellent water resistance and biocompatibility, low cytotoxicity and high drug loading ability, and stable drug release rate, etc., which could be used for water-insoluble drugs carriers, wound dressings, and medical testing equipment. Conclusion: Currently, water-resistant cellulose-based materials were mainly applied in water-insoluble drugs delivery carriers, wound dressing and medical diagnosis and presented great application prospects. However, the contradiction between hydrophobicity and mechanical properties of these reported water-resistant cellulose-based materials limited their wider application in biomedicine such as tissue engineering. In the future, attention will be focused on the higher hydrophobicity of water-resistant cellulose-based materials with excellent mechanical properties. In addition, clinical medical research of water-resistant cellulose-based materials should be strengthened.

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Health Impact of Silver Nanoparticles: A Review of the Biodistribution and Toxicity Following Various Routes of Exposure

International Journal of Molecular Sciences ◽

10.3390/ijms21072375 ◽

2020 ◽

Vol 21 (7) ◽

pp. 2375 ◽

Cited By ~ 20

Author(s):

Zannatul Ferdous ◽

Abderrahim Nemmar

Keyword(s):

Silver Nanoparticles ◽

Biomedical Application ◽

Health Impact ◽

Surgical Instruments ◽

Wound Dressings ◽

Mechanisms Of Toxicity ◽

Biodistribution Studies ◽

Exposure In Vivo

Engineered nanomaterials (ENMs) have gained huge importance in technological advancements over the past few years. Among the various ENMs, silver nanoparticles (AgNPs) have become one of the most explored nanotechnology-derived nanostructures and have been intensively investigated for their unique physicochemical properties. The widespread commercial and biomedical application of nanosilver include its use as a catalyst and an optical receptor in cosmetics, electronics and textile engineering, as a bactericidal agent, and in wound dressings, surgical instruments, and disinfectants. This, in turn, has increased the potential for interactions of AgNPs with terrestrial and aquatic environments, as well as potential exposure and toxicity to human health. In the present review, after giving an overview of ENMs, we discuss the current advances on the physiochemical properties of AgNPs with specific emphasis on biodistribution and both in vitro and in vivo toxicity following various routes of exposure. Most in vitro studies have demonstrated the size-, dose- and coating-dependent cellular uptake of AgNPs. Following NPs exposure, in vivo biodistribution studies have reported Ag accumulation and toxicity to local as well as distant organs. Though there has been an increase in the number of studies in this area, more investigations are required to understand the mechanisms of toxicity following various modes of exposure to AgNPs.

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The Study of Physicochemical Properties and Blood Compatibility of Sodium Alginate-Based Materials via Tannic Acid Addition

Materials ◽

10.3390/ma14174905 ◽

2021 ◽

Vol 14 (17) ◽

pp. 4905

Author(s):

Beata Kaczmarek-Szczepańska ◽

Adrianna Sosik ◽

Anna Małkowska ◽

Lidia Zasada ◽

Marta Michalska-Sionkowska

Keyword(s):

Sodium Alginate ◽

Tannic Acid ◽

Platelet Adhesion ◽

Biomedical Application ◽

Blood Compatibility ◽

Material Surface ◽

Scanning Calorimetry ◽

Atomic Force ◽

Rate Study ◽

Scanning Electron

In this study, sodium alginate-based thin films were modified by the addition of tannic acid. Materials were obtained by solvent evaporation. They were characterized by the observation of its morphology and its surface by scanning electron microscope and atomic force microscope. The thermal properties were studied by differential scanning calorimetry. The concentration of tannic acid released from the material was determined by the Folin–Ciocalteu method. The material safety for biomedical application was determined by the hemolysis rate study in contact with sheep blood as well as platelet adhesion to the material surface. Based on the obtained results, we assume that proposed films based on sodium alginate/tannic acid are safe and may potentially find application in medicine.

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A review of chitosan-, alginate-, and gelatin-based biocomposites for bone tissue engineering

Biomaterials and Tissue Engineering Bulletin ◽

10.33263/bteb534.097109 ◽

2018 ◽

Vol 5 (3-4) ◽

pp. 97-109 ◽

Cited By ~ 1

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Repair ◽

Bone Diseases ◽

Natural Polymers ◽

Antimicrobial Substances ◽

Potential Applications

Bone diseases and injuries have a major impact on the quality of life. Classical treatments for bone repair/regeneration/replacement have various disadvantages. Bone tissue engineering (BTE) received a great attention in the last years. Natural polymers are intensively studied in this field due to their properties (biocompatibility, biodegradability, abundance in nature, high processability). Unfortunately, their mechanical properties are poor, which is why synthetic polymers or ceramics are added in order to provide the optimal compressive, elastic or fatigue strength. Moreover, growth factors, vitamins, or antimicrobial substances are also added to enhance the cell behavior (attachment, proliferation, and differentiation). In this review, new scientific results regarding potential applications of chitosan-, alginate-, and gelatin based biocomposites in BTE will be provided, along with their in vitro and/or in vivo tests.

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Evaluation of the anti-biofilm activities of bacterial cellulose-tannic acid-magnesium chloride composites using an in vitro multispecies biofilm model

Regenerative Biomaterials ◽

10.1093/rb/rbab054 ◽

2021 ◽

Author(s):

Wei He ◽

Zhaoyu Zhang ◽

Jing Chen ◽

Yudong Zheng ◽

Yajie Xie ◽

...

Keyword(s):

Water Absorption ◽

Bacterial Cellulose ◽

Tannic Acid ◽

Magnesium Chloride ◽

Chronic Wounds ◽

Inhibitory Effect ◽

Wound Dressings ◽

Retention Capacity ◽

Biofilm Model

Abstract Chronic wounds are a serious worldwide problem, which are often accompanied by wound infections. In this study, bacterial cellulose (BC)-based composites introduced with tannic acid (TA) and magnesium chloride (BC-TA-Mg) were fabricated for anti-biofilm activities. The prepared composites' surface properties, mechanical capacity, thermal stability, water absorption and retention property, releasing behavior, anti-biofilm activities, and potential cytotoxicity were tested. Results show that TA and MgCl2 particles closely adhered to the nanofibers of BC membranes, thus increasing surface roughness and hydrophobicity of the membranes. While the introduction of TA and MgCl2 did not influence the transparency of the membranes, making it beneficial for wound inspection. BC-TA and BC-TA-Mg composites displayed increased tensile strength and elongation at break compared to pure BC. Moreover, BC-TA-Mg exhibited higher water absorption and retention capacity than BC and BC-TA, suitable for the absorption of wound exudates. BC-TA-Mg demonstrated controlled release of TA and good inhibitory effect on both singly-cultured S. aureus and P. aeruginosa biofilm and co-cultured biofilm of S. aureus and P. aeruginosa. Furthermore, the cytotoxicity grade of BC-TA-6Mg membrane was eligible based on standard toxicity classifications. These indicated that BC-TA-Mg is potential to be used as wound dressings combating biofilms in chronic wounds.

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Artificial skin composites

Annales Universitatis Mariae Curie-Sklodowska sectio AA – Chemia ◽

10.17951/aa.2018.73.1.51-60 ◽

2019 ◽

Vol 73 (1) ◽

pp. 51

Author(s):

Agata Ładniak

Keyword(s):

Wound Dressings ◽

Skin Damage ◽

Skin Substitutes ◽

Significant Deterioration ◽

Form And Function ◽

Potential Applications ◽

And Function ◽

The Impact

Skin injuries are a health problem and can lead to serious, significant deterioration in the quality of life and, consequently, even illness and disability. Therefore, after wounding, immediate regeneration of the tissue is necessary to avoid further complications and pathogenesis. Consequently, many wound healing strategies have been developed, leading to the progress in constructing of multifunctional tissue substitutes for the skin, biomembranes, scaffolds and intelligent dressings. The field of science focusing on the creation of the above-mentioned products is tissue engineering (TE). Its main goal is to find a system that is able to replace or be a model that perfectly mimics the form and function of the skin. Research carried out on such constructs is mainly based on the analysis of mechanical properties (porosity, elasticity), as well as the assessment of the impact of individual components on processes related to the formation of new tissue as cell proliferation and differentiation, proliferation, angiogenesis - through in vivo studies (using animal models: mice, New Zealand rabbits) and in vitro (most often using mouse fibroblasts - L929). Skin constructions may have potential applications as wound dressings or skin substitutes in cases of severe skin damage.

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In Vitro Hemocompatibility and Cytotoxicity Evaluation of Halloysite Nanotubes for Biomedical Application

Journal of Nanomaterials ◽

10.1155/2015/685323 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 10

Author(s):

Hao-Yang Liu ◽

Lei Du ◽

Yan-Teng Zhao ◽

Wei-Qun Tian

Keyword(s):

Biomedical Application ◽

Blood Compatibility ◽

A549 Cells ◽

Fluorescein Isothiocyanate ◽

Halloysite Nanotubes ◽

In Vitro Test ◽

Structures And Properties ◽

Vitro Test ◽

Plasma Recalcification Time

Halloysite nanotubes (HNTs), due to their unique structures and properties, may play an important role in biomedical applications. In vitro test is usually conducted as a preliminary screening evaluation of the hemocompatibility and cytotoxicity of HNTs for its short term consuming, convenience, and less expense. In this work, HNTs were processed with anticoagulated rabbit blood to detect its blood compatibility. The result of hemolysis test shows that the hemolysis ratios are below 0.5%, indicating nonhemolysis of HNTs. Plasma recalcification time suggests that HNTs are dose-dependently contributing to blood coagulation in platelet poor plasma (PPP). The effect of platelet activation caused by HNTs was also examined by scanning electron microscopy (SEM). Meanwhile, HNTs were labeled with fluorescein isothiocyanate (FITC) to observe its intracellular distribution in A549 cells under confocal microscopy. CCK-8 test and TUNEL test of HNTs at different concentration levels were performed in vitro, respectively. Therefore, the potential usage of HNTs in medicine may be very meaningful in oral dosing, dermal application, dental uses, or medical implants.

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Carbon Nanofibers Coated with Silicon/Calcium-Based Compounds for Medical Application

Journal of Nanomaterials ◽

10.1155/2019/7172641 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11

Author(s):

Wojciech Smolka ◽

Elzbieta Dlugon ◽

Piotr Jelen ◽

Wiktor Niemiec ◽

Agnieszka Panek ◽

...

Keyword(s):

Carbon Nanofibers ◽

Biomedical Application ◽

Medical Application ◽

Dip Coating ◽

Bone Tissue Regeneration ◽

Alp Activity ◽

Potential Applications ◽

Biological Tests ◽

Artificial Plasma ◽

Calcium Compounds

The aim of this work was to develop a method for the manufacture of carbon nanofibers in the form of mats containing silicon and calcium compounds with potential biomedical application. Carbon nanofibers (ECNF) were prepared from the electrospun polyacrylonitrile (PAN) nanofibers. The electrospun polymer nanofibers were heat treated up to 1000°C to obtain carbon nanofibers. The surface of ECNF was covered with a silica-calcium sol (ECNF+Si/Ca) by dip-coating technique followed by the stabilization process. Both types of carbon nanofibers, i.e., the as-received and covered with the sol, were tested to confirm their osteoconductive properties. Biological tests were performed, including genotoxicity, cytotoxicity, and alkaline phosphatase (ALP) activity. Morphology of adhering cells to nanofiber surface was described. The nanofibers were subjected to a bioactivity test in contact with SBF artificial plasma. Biological tests have revealed that the nanofiber-modified ECNF+Si/Ca in contact with osteoblast cells were biocompatible, and the level of cytotoxicity was lower compared to the control. The ALP activity of the modified nanofibers was higher than nonmodified nanofibers and indicates potential applications of such carbon materials in the form of mats as a substrate for bone tissue regeneration.

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Improving Sodium Alginate Films Properties by Phenolic Acid Addition

Materials ◽

10.3390/ma13132895 ◽

2020 ◽

Vol 13 (13) ◽

pp. 2895

Author(s):

Beata Kaczmarek

Keyword(s):

Tensile Strength ◽

Sodium Alginate ◽

Tannic Acid ◽

Color Change ◽

Antioxidant Properties ◽

Acid Mixture ◽

Packaging Materials ◽

Natural Polymers ◽

Film Forming ◽

Cross Linker

Currently, packaging materials constitute a group of the most commonly used products. Natural polymers are widely tested as potential packaging materials to replace traditional plastics. Sodium alginate is eco-friendly and reveals effective film-forming properties whereas tannic acid has been proposed as a sodium alginate cross-linker. Thin films of sodium alginate/tannic acid were obtained by solvent evaporation. Interactions between the components were determined as well as the maximum tensile strength and color change after contact with different solutions. Improvement in the physicochemical properties of the obtained films was noticed. Moreover, such films showed antioxidant properties. It may be assumed that materials based on a sodium alginate/tannic acid mixture are promising alternatives to traditional packaging materials.

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