High-temperature sample heating in an electron microscope

Vacuum ◽  
1972 ◽  
Vol 22 (3) ◽  
pp. 124
Author(s):  
J. L. Farrant ◽  
J. D. McLean

For electron microscope techniques such as ferritin-labeled antibody staining it would be advantageous to have available a simple means of thin sectioning biological material without subjecting it to lipid solvents, impregnation with plastic monomers and their subsequent polymerization. With this aim in view we have re-examined the use of protein as an embedding medium. Gelatin which has been used in the past is not very satisfactory both because of its fibrous nature and the high temperature necessary to keep its solutions fluid. We have found that globular proteins such as the serum and egg albumins can be cross-linked so as to yield blocks which are suitable for ultrathin sectioning.


2021 ◽  
Vol 92 (2) ◽  
pp. 025111
Author(s):  
T. Schmidt ◽  
D. Schlander ◽  
V. Jüchter ◽  
J. Baranyai ◽  
F. Neuberger ◽  
...  

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 398
Author(s):  
Chihao Liu ◽  
Jiajian Chen

At present, the research on the high temperature degradation of concrete usually focuses on only the degradation of concrete itself without considering the effect of the plastering layer. It is necessary to take into account the influence of the plastering layer on the high temperature degradation of concrete. With an increase in the water/cement ratio, the explosion of concrete disappeared. Although increasing the water/cement ratio can alleviate the cracking of concrete due to lower pressure, it leads to a decrease in the mechanical properties of concrete after heating. It is proved that besides the water/cement ratio, the apparent phenomena and mechanical properties of concrete at high temperature can be affected by the plastering layer. The plastering layer can relieve the high temperature cracking of concrete, and even inhibit the high temperature explosion of concrete with 0.30 water/cement ratio. By means of an XRD test, scanning electron microscope test and thermogravimetric analysis, it is found that the plastering layer can promote the rehydration of unhydrated cement particles of 0.30 water/cement ratio concrete at high temperature and then promote the mechanical properties of concrete at 400 °C. However, the plastering layer accelerated the thermal decomposition of C-S-H gel of concrete with a water/cement ratio of 0.40 at high temperature, and finally accelerate the decline of mechanical property of concrete. To conclude, the low water/cement ratio and plastering layer can delay the deterioration of concrete at high temperature.


1976 ◽  
Vol 98 (4) ◽  
pp. 1125-1134 ◽  
Author(s):  
R. Komanduri ◽  
M. C. Shaw

Attritious wear of silicon carbide in simulated grinding tests against a cobalt base superalloy at high speed and extremely small feed rate was studied using a scanning electron microscope (SEM) and an auger electron spectroscope (AES). In many cases the wear area of silicon carbide was found to be concave rather than planar in shape. Several microcracks and grain boundary fracture were also observed. No evidence of metal build-up was observed on silicon carbide which was not the case with aluminum oxide. AES study of the rubbed surface on the work material and transmission electron microscope (TEM) investigation of the wear debris suggest that attritious wear of silicon carbide is due to one or more of the following mechanisms: 1 – Preferential removal of surface atoms on the abrasive, layer by layer, by oxidation under high temperature and a favorably directed shear stress; 2 – disassociation of silicon carbide at high temperature and (a) diffusion of silicon into the work material and formation of metal silicides and (b) diffusion of carbon into the work material and formation of unstable metal carbides (in the present case Ni3C and Co3C) which decompose during cooling to metal and carbon atoms; 3 – pinocoidal cleavage fracture of silicon carbide on basal planes c(0001) resulting in the removal of many micron-sized crystallites.


2021 ◽  
Vol 1016 ◽  
pp. 379-384
Author(s):  
Eider Del Molino ◽  
Teresa Gutierrez ◽  
Mónica Serna-Ruiz ◽  
Maribel Arribas ◽  
Artem Arlazarov

The aim of this work was to study the influence of quenching and partitioning temperatures combined with various levels of Mn and Ni contents on the austenite stabilization along the quenching and partitioning (Q&P) cycle. Three steels with 2 wt.%, 4 wt.% and 6 wt.% manganese and one steel with 2 wt.% nickel content were investigated. Phase transformation temperatures and critical cooling rates were obtained experimentally using dilatometer for each alloy. Q&P cycles with different quenching and partitioning temperatures were also done in dilatometer, thus, allowing monitoring of the expansion/contraction during the whole Q&P cycle. Microstructure characterization was performed by means of a Scanning Electron Microscope and X-Ray Diffraction to measure retained austenite content. It was found that, strongly depending on the Q&P conditions, austenite stabilization or decomposition occurs during partitioning and final cooling. In case of high partitioning temperature cycles, austenite reverse transformation was observed. Certain cycles resulted in a very effective austenite stabilization and interesting microstructure.


2019 ◽  
Vol 25 (S2) ◽  
pp. 1540-1541
Author(s):  
Daan Hein Alsem ◽  
James Horwath ◽  
Julio Rodriguez-Manzo ◽  
Khim Karki ◽  
Eric Stach

2019 ◽  
Vol 27 (06) ◽  
pp. 1950155
Author(s):  
KWANG-HU JUNG ◽  
SEONG-JONG KIM

The corrosion characteristics of Inconel 600 were investigated at 650∘C in air and 76%[Formula: see text]%[Formula: see text]%[Formula: see text]%SO2 gas environment up to 500[Formula: see text]h. Specimens exposed to each condition were characterized by weight gain, scanning electron microscope, energy dispersive X-ray spectroscopy and X-ray diffraction. The oxide structure consisting of the thin Cr2O3 layer and Cr2O3 nodules was observed, which increased the weight gain of specimens. In the SO2-bearing gas, it showed a bigger weight gain due to the coarsening of Cr2O3 nodules. Therefore, it was suggested that the sulfur-accelerated coarsening of Cr2O3 nodules at the high temperature.


2020 ◽  
Vol 155 ◽  
pp. 01005
Author(s):  
Weiwei Liu ◽  
Yuanyuan Guo ◽  
Mai Zhang ◽  
Jian Zhang

A Re-containing single-crystal superalloy was used to research the high temperature low stress creep behavior. Transmission electron microscope, scanning electron microscope and some other research methods are employed. The results and analysis are summarized below: Two mechanisms for the steady creep are found in this experiment. The volume fraction of pores after creep test at 1100°C increased more than 2 times compared with that before test, but the increasing at 1000°C is relatively small, which reveals that temperature has an great influence on the formation of pore during creep; There are two types of pores associated with fracture during the creep process. One is the casting shrinkage located between the interdentritic, which is formed in the solidification of the alloy. Another type of pore is nucleated and growing during the creep deformation.


2013 ◽  
Vol 804 ◽  
pp. 333-336
Author(s):  
Yun Jian Jiang ◽  
Xiang Feng Zheng ◽  
Rong Gang Xue ◽  
Guo Zhen Dong ◽  
Ji Feng Zhao ◽  
...  

Through methods, such as microscope analysis, chemical analysis, metallography examination and scanning electron microscope etc, the causes resulting in high temperature reheater tube cracking of station boiler have been analyzed. The result indicates the crack is reheat crack, and structure stress, higher hardness and excessive welding residual stress are the primary inducement of tube joint crack.


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