Fertility in the ewe after artificial insemination with fresh and frozen semen at the induced oestrus, and influence of the photoperiod on the semen quality of the ram

1979 ◽  
Vol 6 (2) ◽  
pp. 153-166 ◽  
Author(s):  
G Colas
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Frozen Semen

scholarly journals Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program

2020 ◽  
Vol 13 (12) ◽  
pp. 2649-2657
Author(s):  
Berlin Pandapotan Pardede ◽  
Muhammad Agil ◽  
Yudi Yudi ◽  
Iman Supriatna
Keyword(s):  
Dna Fragmentation ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Fertility Rate ◽  
Breeding Program ◽  
Sperm Viability ◽  
Production Site ◽  
Frozen Semen ◽  
Acrosome Integrity

Background and Aim: Various factors can reduce the quality of semen used for artificial insemination and have an impact on fertility decline, such as poor handling during frozen semen distribution. This study was aimed at assessing the quality of frozen-thawed semen after distribution in the field and its importance in maintaining fertility. Materials and Methods: The Brahman Cross (BX) breeding program of PT Lembu Jantan Perkasa, Indonesia, was used. This program was preferred due to its adherence to guidelines that limit the effects of extraneous factors that may affect semen quality. Frozen-thawed semen samples from eight bulls with the same production code were analyzed and compared between the production site (artificial insemination [AI] center) and the field (BX breeding program). Total and progressive motility (PM) of sperm were determined using computer-assisted semen analysis. Plasma membrane integrity (PMI) was assessed using hypoosmotic swelling test, sperm viability using Eosin-Nigrosin staining, acrosome integrity using trypan blue-Giemsa staining, morphological abnormalities using William staining, and DNA fragmentation using toluidine blue staining. The fertility rate was determined using the conception rate (%) derived from AI data based on 502 AI services and 478 cows in the BX breeding program. A t-test was used to compare the quality of frozen-thawed semen before and after distribution. The relationship between the qualities of frozen semen after distribution in the field with fertility was analyzed using Pearson correlation. Results: There was no significant difference (p>0.05) in the quality of frozen-thawed semen (sperm motility, PMI, viability, acrosome integrity, abnormalities, and DNA fragmentation) between the production site (AI center) and after distribution in the field (BX breeding program). The semen met the minimum standards for AI programs. Total motility (r=0.986), PM (r=0.961), sperm viability (r=0.971), PMI (r=0.986), and acrosome integrity (r=0.992) were all positively correlated (p<0.05) with fertility rate; while sperm abnormalities (r=-0.996) and sperm DNA fragmentation (r=0.975) were negatively correlated (p<0.05) with fertility rate. Conclusion: The study showed that to achieve the maximal and optimal fertility rate in bulls in an AI program, the overall quality of frozen-thawed semen in all aspects is critical. This can be achieved if the handling during distribution and storage, as well as the various factors that may affect the quality of semen in the field, can be controlled properly.

scholarly journals Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

2018 ◽  
Vol 43 (1) ◽  
pp. 26 ◽  
Author(s):  
H. C. Mahendra ◽  
D. Samsudewa ◽  
Y. S. Ondho
Keyword(s):  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Animal Husbandry ◽  
Mean Value ◽  
National Standard ◽  
Frozen Semen ◽  
Standard Values

This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value. 

scholarly journals The The Effect of Age and Breed on The Quality of Bull Semen in The Regional Artificial Insemination Centre

2021 ◽  
pp. 132-136
Author(s):  
Agung Budiyanto ◽  
Makruf Arif ◽  
Mandala Phivi Whelma Alfons ◽  
Rifia Tiara Fani ◽  
Ardian Faiz Hafid ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Age Groups ◽  
Bull Semen ◽  
Sperm Abnormalities ◽  
Frozen Semen ◽  
Cattle Reproduction ◽  
Effect Of Age ◽  
Artificial Vagina

The success of beef cattle reproduction development in Indonesia very much depends on the quality of the semen produced by the Indonesian Institute for Artificial Insemination. Good quality bull will benefit farmers, frozen semen producers, and the country in large. This study aimed to determine effect of age and breed on the libido and quality of bull semen collected in the Regional Artificial Insemination Center (RAIC). This study used PO, Simmental, and Brahman Bull in RAIC Yogyakarta, with a total amount of 15, aged 4-7 years old. Semen was collected using an artificial vagina, semen evaluation used a spectrophotometer, and eosin-nigrosin staining method used for sperm viability and abnormalities examination. The result of this study indicated that bull breed did not affect the libido value and the semen quality of bulls (P>0,05). Volume, concentration, motility, and viability in various age groups did not show significant differences (P>0,05), but age groups had a significant effect on sperm abnormalities (P<0,05). The group of bulls aged 5-6 years showed the best quality compared to other groups, with the volume 4,58 ± 1,37 ml, concentration 1,91 ± 0,36 x109/ml, motility 3,80 ± 1,30, viability 89.83 ± 10.69%, and sperm abnormalities 2.51 ± 0.81%. It can be concluded that breed did not affect the bull libido and semen quality, while age had an effect on bull sperm abnormalities.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals Comparative study of semen quality traits between Etawah grade and Senduro bucks

2021 ◽  
Vol 888 (1) ◽  
pp. 012022
Author(s):  
N Isnaini ◽  
A A Hakim ◽  
D Amertaningtyas ◽  
H E Sulistyo ◽  
A Irsyammawati ◽  
...  
Keyword(s):  
Comparative Study ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
T Test ◽  
Quality Traits ◽  
Spermatozoa Motility ◽  
Frozen Semen ◽  
Semen Volume

Abstract This study compared the semen quality traits between Etawah grade (EGB) and Senduro bucks (SB) raised in Singosari National Artificial Insemination Center, Indonesia. A total of 96 ejaculated semen were collected from three EGB and three SB with an average age of three years. The ejaculates were evaluated for semen volume (SV), spermatozoa concentration (SC), total spermatozoa (TS), spermatozoa motility (SM), post-thawing spermatozoa motility (PTSM), the recovery rate of spermatozoa motility (RRSM), and frozen semen production (FSP). Data of SV, SM, PTSM, and RRSM were compared using the Mann-Whitney U test. While the data of SC, TS, and FSP were analyzed using an independent t-test. The results showed that SV, SM, PTSM, and RRSM were not significantly differed (P>0.05) between breeds. Whereas, SC, TS, and FSP of EGB were significantly higher (P<0.05) than SB. Therefore, it can be concluded that Etawah grade bucks can produce higher total spermatozoa and as a result higher frozen semen production for artificial insemination as compared to Senduro bucks.

scholarly journals Repeatability and Phenotypic Correlation Among Semen Quality Traits in Holstein Bulls

2018 ◽  
Vol 42 (4) ◽  
Author(s):  
Argi Argiris ◽  
Siswanto Imam Santoso ◽  
Yon Supri Ondho ◽  
Edy Kurnianto
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Intraclass Correlation ◽  
Correlation Method ◽  
Phenotypic Correlation ◽  
Quality Traits ◽  
Frozen Semen ◽  
Holstein Bulls ◽  
Fresh Semen ◽  
Selection Of

The purpose of this research was to analysis the value of repeatability and correlation among the traits affecting the production of frozen semen from Holstein’s bull in Indonesia. Repeatability and correlation were calculated based on the data of frozen semen production of 15.699 records from 44 Holstein bulls at Singosari Artificial Insemination Center (SAIC) and 8.935 records from 39 Holstein bulls at Lembang Artificial Insemination Center (LAIC). Repeatability for volume, motility, fresh semen concentration and frozen semen production was evaluated by intraclass correlation method. The repeatability values of LAIC for volume, motility, fresh semen concentration and frozen semen production were 0.60; 0.54; 0.37 and 0.47. The repeatability values of SAIC for volume, motility, fresh semen concentration and frozen semen production were 0.54; 0.30; 0.43 and 0.29. The linear correlation value between volume, motility and fresh semen concentration with the amount of semen produced per collections were 0.41, 0.36, and 0.58. Concentration was the most factors influencing the number of frozen semen produced. The effectiveness of the selection of Holstein's frozen semen producing could be determined by the value of repeatability and the phenotypic correlation among semen quality traits such as volume, motility, concentration and frozen semen production.

scholarly journals A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

2016 ◽  
Vol 85 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Marina Anastasia Karageorgiou ◽  
Georgios Tsousis ◽  
Constantin M. Boscos ◽  
Eleni D. Tzika ◽  
Panagiotis D. Tassis ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Medium Term ◽  
Semen Collection ◽  
Chromatin Integrity ◽  
Boar Semen ◽  
Short Time

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16–18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research.

scholarly journals Utilization Of Frozen Goat Semen With Addition Sweet Orange Essential Oil To Improve Genetic Quality in Ujung Teran Village

2019 ◽  
Vol 1 (2) ◽  
pp. 170-174
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Community Service ◽  
Artificial Insemination ◽  
Sweet Orange ◽  
Genetic Quality ◽  
Know How ◽  
Frozen Semen ◽  
And Training ◽  
Orange Essential Oil

One of the causes of underdeveloped goats is low genetic quality. The way that can be done to improve the genetic quality of goats is to do cross-breeding with superior goats through Artificial Insemination. The community service aims to increase the knowledge of goat farmers in Ujung Teran Village, Langkat Regency on how to make goat frozen semen using tris yolk extender plus sweet orange essential oil for Artificial Insemination program along with the benefits that can be obtained. The method of service is by giving lectures and training to goat farmers. The results obtained from the implementation of this program are that farmers know how to make frozen semen of goat using sweet orange essential oil on the tris yolk extender and are willing to make Artificial Insemination in their goat cattle.

scholarly journals Effect of Various Thawing Times and Temperatures on Frozen Semen Quality in Plastic Straws of Artificial Insemination of Bulls in Iraq

2018 ◽  
Vol 30 (2) ◽  
pp. 99-102
Author(s):  
Asaad H. I. Al-Edany
Keyword(s):  
Artificial Insemination ◽  
Light Microscope ◽  
Semen Quality ◽  
Practical Method ◽  
Sperm Cells ◽  
Frozen Semen

The present study was designed to determine the best practical method of semen thawing which could be applied to frozen semen of bulls in straw produced by Artificial Insemination Center, Iraq. Frozen semen was thawed at 15°C for 30 sec, 40°C for 10 sec and 50°C for 5 sec. individual  motility, live, and dead sperm cells was assessed using light microscope. Results revealed that number, motility, and  live of sperm cells, were significantly higher at the 50°C for 5 sec than other thawing temperatures In conclusion, it is recommended to use a temperatures of   50°C for 5 sec and to dissolve  the frozen semen because it gave the best quality semen after thawing  and easy to utilize in field.

scholarly journals Effect of preservation time on the quality of frozen semen in indigenous rams

2015 ◽  
Vol 44 (1) ◽  
pp. 10-15 ◽  
Author(s):  
BBA Mahmuda ◽  
Azizun Nesa ◽  
BF Zohara ◽  
MGS Alam ◽  
FY Bari
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Volume Density ◽  
Normal Morphology ◽  
Mean Values ◽  
Frozen Semen ◽  
Significant Difference ◽  
The Mean ◽  
Fresh Semen

The study was carried out to observe the effects of preservation time on the quality of frozen semen of indigenous rams. Semen was collected using AV once a week from 4 rams. Tris based with 10% egg yolk and 7% glycerol extender was used to extend and freezing the semen. Fresh semen was evaluated for volume, density, mass motility and concentration, and mean values were observed as 0.8±0.2ml, 3.0±0.3, 3.2±0.7, 3.9±0.7×109/ml, respectively. Significant difference (p<0.05) was found for all the parameters among the rams. Mean values of motility, viability and normal morphology percentages were 83.3±4.3%, 88.2±4.4%, 84.2±3.5% in fresh semen while those of chilled semen at 40C were 74.7±2.3, 78.8±4.9 and 79.2±2.9%, respectively. For all the parameters, significant (p<0.05) difference was found among the rams. Frozen sperm motility was observed after thawing at 39-400C for 14-15 seconds. The mean motility, viability and normal morphology percentages after freezing for 24hrs, 7, 15 and 30 days of duration were 39.8±3.1, 41.1±4.3, 40.1±4.1 and 39.4±2.9%; 44.5±2.5, 45.3±2.8, 44.6±2.8 and 43.9±2.8%; 71.0±2.0, 71.7±1.5, 70.7±1.7 and 70.3±1.8%, respectively and values did not decrease significantly (p>0.05) with the increasing time of preservation. Non significantly decrease of the semen quality with advance of preservation time indicates the suitability of the protocol used for freezing of indigenous ram semen in Bangladesh.DOI: http://dx.doi.org/10.3329/bjas.v44i1.23113            Bang. J. Anim. Sci. 2014. 44 (1): 10-15

Sign in / Sign up

Export Citation Format

Share Document