Matrix Effects | ScienceGate

A simple, sensitive, rapid and economic high performance thin layer chromatographic method and a mass spectroscopic assay method has been developed for the quantification of telmisartan and hydrochlorthiazide combination in human plasma. The internal standards and analytes were extracted from human plasma by solid-phase extraction with HLB Oasis1cc (30mg) catridges. The scanning and optimization for the samples are done using methanol: water (50:50). The samples were chromatographed using reverse phase chromatography with C-18 column of different manufacturers like Ascentis C18 (150×4. 6, 5µ) using the buffer system Acetonitrile: Buffer (80:20%v/v) which consist of 2±0. 1Mm ammonium format at a flow rate of 0. 7ml/min at a column oven temperature 35±10c. The internal standard used was hydrochlorthiazide13c1, d2 and telmisartand3. The extraction techniques include conditioning, loading, washing and elution, drying followed by reconstitution of the dried samples. The volume injected was 10µl with the retention time of 3-4 min for telmisartan, 1-2 min for hydrochlorthiazide and for the internal standards the retention time was 3-4 min for telmisartand3 and 1-2 min for hydrochlorthiazide c13d2. The rinsing solution was Acetonitrile: HPLC grade water in the ratio (50:50). The above developed method was validated using various parameters like selectivity and sensitivity, accuracy and precision, matrix effects, % recovery and various stability studies. The method was proved to be sensitive, accurate, precise and reproducible. The preparation showed high recovery for the quantitative determination of telmisartan and hydrochlorthiazide in human plasma.

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Analytical Methods for Quantification and Identification of Intact Glucosinolates in Arabidopsis Roots Using LC-QqQ(LIT)-MS/MS

Metabolites ◽

10.3390/metabo11010047 ◽

2021 ◽

Vol 11 (1) ◽

pp. 47

Author(s):

Kourosh Hooshmand ◽

Inge S. Fomsgaard

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Crude Extract ◽

Matrix Effects ◽

Critical Role ◽

Reversed Phase ◽

Biologically Active ◽

Natural Soil ◽

Chromatography Method ◽

Arabidopsis Root

Glucosinolates are biologically active secondary metabolites in Brassicaceae plants that play a critical role in positive and negative interactions between plants and root-associated microbial communities. The aim of this study was to develop a reversed-phase liquid chromatography method to quantify and identify intact glucosinolates in the root of Arabidopsis thaliana (Arabidopsis) grown in non-sterile natural soil by using liquid chromatography-hybrid triple quadruple-linear ion trap (LC-QqQ(LIT)) mass spectrometry. The Synergi Fusion C18-based column was found to be effective for sufficient retention and separation of nine intact glucosinolates without the need for time-consuming desulfation or ion-pairing steps. Method validation results showed satisfactory inter-day and intra-day precision for all glucosinolates except for 4-hydroxyglucobrassicin. Good inter-day and intra-day accuracy and recovery results were observed for glucoiberin, gluconapin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrassicin. However, for 4-hydroxyglucobrassicin, glucoraphanin and glucoerucin corrections to quantification results might be necessary since the recovery and accuracy results were not optimal. Matrix effects were shown to have a negligible effect on the ionization of all target analytes. The established liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was applied to quantify target intact glucosinolates in Arabidopsis root crude extract of four different wild-type accessions where differences in terms of concentration and composition of intact glucosinolates were observed. Employment of sensitive and selective precursor ion survey scan of m/z 97 in combination with the information-dependent acquisition (IDA) of the enhanced product ion (EPI) dependent scan (Prec97-IDA-EPI) using LC-QqQ(LIT) provided high confidence in structural characterization of diverse intact glucosinolate profiles in complex Arabidopsis root crude extract.

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A solid-phase extraction method that eliminates matrix effects of complex pulp mill effluents for the analysis of lipophilic wood extractives

Nordic Pulp & Paper Research Journal ◽

10.1515/npprj-2020-0039 ◽

2020 ◽

Vol 35 (4) ◽

pp. 577-588

Author(s):

Sebastian España Orozco ◽

Philipp Zeitlinger ◽

Karin Fackler ◽

Robert H. Bischof ◽

Antje Potthast

Keyword(s):

Solid Phase Extraction ◽

Matrix Effects ◽

Solid Phase ◽

Internal Standard ◽

Pulp Mill ◽

Phase Extraction ◽

Wood Extractives ◽

Wood Extractive ◽

Pulp Mill Effluents ◽

The Impact

AbstractThe extraction of lipophilic wood extractives from pulp and paper process waters proves to be a challenging task, due to harsh and alternating process and sample conditions. This study has determined the potential use of polymeric sorbents for solid-phase extraction (SPE) and compared to classical silica-based reversed-phase packed columns, with polymeric hydrophilic-lipophilic balanced (HLB) cartridges being the sorbent with the most potential. Recovery functions were obtained with an internal standard mixture representative for the main lipophilic wood extractive groups, which are fatty acids and alcohols, sterols, sterol esters and triglycerides. The impact of pH, sample volume and sample matrix, expressed as TOC and cations, on the retention behavior of lipophilic extractives during SPE of industrial samples were determined with polymeric HLB sorbent. High variations in the composition of pulp mill matrices led to different optimal extraction conditions. Thus, a new SPE protocol was developed, which bypasses matrix interferences and omits the loss of analytes due to sample preparation. The method is applicable to different pulp mill effluents with large discrepancies in pH and sample matrices, resulting in recoveries >90 % with RSD <5 % for all lipophilic wood extractives.

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Improving the bioavailability of oil-soluble vitamins by optimizing food matrix effects: A review

Food Chemistry ◽

10.1016/j.foodchem.2021.129148 ◽

2021 ◽

Vol 348 ◽

pp. 129148

Author(s):

Yunbing Tan ◽

David Julian McClements

Keyword(s):

Matrix Effects ◽

Food Matrix

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A NOVEL STRATEGY FOR DIRECT ELEMENTAL DETERMINATION USING LASER-INDUCED BREAKDOWN SPECTROSCOPY: FLUENCE CALIBRATION

Journal of Analytical Atomic Spectrometry ◽

10.1039/d1ja00229e ◽

2021 ◽

Author(s):

Raquel C Machado ◽

Diego Victor Babos ◽

Daniel Fernandes Andrade ◽

Edenir Rodrigues Pereira-Filho

Keyword(s):

Quantitative Analysis ◽

Matrix Effects ◽

Calibration Method ◽

Laser Induced Breakdown Spectroscopy ◽

Breakdown Spectroscopy ◽

Elemental Determination ◽

Laser Induced Breakdown ◽

Direct Solid Analysis ◽

Novel Strategy ◽

Direct Solid

Quantitative analysis requires several efforts to obtain an adequate calibration method to overcome matrix effects employing direct solid analysis by laser-induced breakdown spectroscopy (LIBS). To this end, in this study,...

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Application of Bar Adsorptive Microextraction for the Determination of Levels of Tricyclic Antidepressants in Urine Samples

Molecules ◽

10.3390/molecules26113101 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3101

Author(s):

Mariana N. Oliveira ◽

Oriana C. Gonçalves ◽

Samir M. Ahmad ◽

Jaderson K. Schneider ◽

Laiza C. Krause ◽

...

Keyword(s):

Tricyclic Antidepressants ◽

Matrix Effects ◽

Activated Carbons ◽

Process Efficiency ◽

Gas Chromatography Mass Spectrometry ◽

Selected Ion Monitoring ◽

Experimental Conditions ◽

Analytical Methodology ◽

Low Efficiency

This work entailed the development, optimization, validation, and application of a novel analytical approach, using the bar adsorptive microextraction technique (BAμE), for the determination of the six most common tricyclic antidepressants (TCAs; amitriptyline, mianserin, trimipramine, imipramine, mirtazapine and dosulepin) in urine matrices. To achieve this goal, we employed, for the first time, new generation microextraction devices coated with convenient sorbent phases, polymers and novel activated carbons prepared from biomaterial waste, in combination with large-volume-injection gas chromatography-mass spectrometry operating in selected-ion monitoring mode (LVI-GC-MS(SIM)). Preliminary assays on sorbent coatings, showed that the polymeric phases present a much more effective performance, as the tested biosorbents exhibited low efficiency for application in microextraction techniques. By using BAμE coated with C18 polymer, under optimized experimental conditions, the detection limits achieved for the six TCAs ranged from 0.2 to 1.6 μg L−1 and, weighted linear regressions resulted in remarkable linearity (r2 > 0.9960) between 10.0 and 1000.0 μg L−1. The developed analytical methodology (BAμE(C18)/LVI-GC-MS(SIM)) provided suitable matrix effects (90.2–112.9%, RSD ≤ 13.9%), high recovery yields (92.3–111.5%, RSD ≤ 12.3%) and a remarkable overall process efficiency (ranging from 84.9% to 124.3%, RSD ≤ 13.9%). The developed and validated methodology was successfully applied for screening the six TCAs in real urine matrices. The proposed analytical methodology proved to be an eco-user-friendly approach to monitor trace levels of TCAs in complex urine matrices and an outstanding analytical alternative in comparison with other microextraction-based techniques.

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