scholarly journals Preterm Labor is a Distinct Process from Term Labor following Computational Analysis of Human Myometrium.

2021 ◽  
Author(s):  
Jason Phung ◽  
Carol Wang ◽  
Jocelyn Reeders ◽  
Eng-Cheng Chan ◽  
Carlos Riveros ◽  
...  
Keyword(s):  
Preterm Labor ◽  
Computational Analysis ◽  
Human Myometrium ◽  
Distinct Process ◽  
Term Labor

scholarly journals Microarray Analysis of Uterine Gene Expression in Mouse and Human Pregnancy

2003 ◽  
Vol 17 (8) ◽  
pp. 1454-1469 ◽  
Author(s):  
Kathleen E. Bethin ◽  
Yoshihiko Nagai ◽  
Robert Sladek ◽  
Minoru Asada ◽  
Yoel Sadovsky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Preterm Labor ◽  
Human Myometrium ◽  
Oligonucleotide Microarrays ◽  
Mouse Uterus ◽  
Primary Mechanism ◽  
Study Gene Expression ◽  
Induction Of Enzymes ◽  
Labor Patterns ◽  
Term Labor

Abstract Improved care of infants born prematurely has increased their survival. However, the incidence of preterm labor has not changed. To understand the processes involved in preterm labor, we used oligonucleotide microarrays to study gene expression in murine and human uterus during pregnancy. The induction of enzymes for prostaglandin synthesis was used as a marker for important changes during pregnancy because prostaglandins strongly contribute to both human and murine labor. We identified 504 genes that changed at least 2-fold between d 13.5 and 19.0 in the gravid mouse uterus. In the pregnant human myometrium, we found 478 genes that changed at least 2-fold in either term or preterm labor compared with preterm nonlabor specimens and 77 genes that significantly varied in both preterm and term labor. Patterns of gene regulation within functional groups comparing human preterm and term labor were similar, although the magnitude of change often varied. Surprisingly, few genes that changed significantly throughout pregnancy were the same in the mouse and human. These data suggest that functional progesterone withdrawal in human myometrium may not be the primary mechanism for labor induction, may implicate similar mechanisms for idiopathic preterm and term labor in humans, and may identify novel targets for further study.

scholarly journals IL-27 Mediates Neutrophils Infiltration at the Maternal and Fetal Interface in Preterm Labor With Infection

2021 ◽  
Author(s):  
youwen mei ◽  
Dongni Huang ◽  
Yuxin Ran ◽  
Zheng Liu ◽  
Lulu Wang ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Preterm Labor ◽  
Neutrophil Infiltration ◽  
Term Labor

Abstract Objective To reveal the role of IL-27 in neutrophils infiltration at the maternal and fetal interface in preterm labor with infection (PTLI). Methods The expression of IL-27 receptor and the number of neutrophils (MPO + cells) at the maternal and fetal interface of pregnant women were compared between PTLI group and term labor (TL) group. Using LPS-induced preterm labor IL-27Rα-/- mice, the role of IL-27 in neutrophils infiltration at the maternal and fetal interface was investigated. Results The expression of IL-27Rα and neutrophils number at the maternal and fetal interface in the PTLI group were higher than those in the TL group in pregnant women. Compared with PBS-treated mice, LPS-treated mice had increased infiltrating neutrophils at the maternal and fetal interface. Meanwhile, LPS-induced IL-27Rα-/- mice had less neutrophil infiltration than LPS-induced WT mice. Conclusion IL-27 promotes neutrophil infiltration at the maternal and fetal interface in PTLI.

scholarly journals Difference of Maternal Serum Interleukin-8 in Preterm Labor and Full Term Labor

2016 ◽  
Author(s):  
Komang W Budiartha
Keyword(s):  
Preterm Labor ◽  
Cross Sectional Study ◽  
Maternal Serum ◽  
T Test ◽  
Full Term ◽  
Interleukin 8 ◽  
Cross Sectional ◽  
The Mean ◽  
The Difference ◽  
Term Labor

Objective: To determine the difference of maternal interleukin-8 (IL-8) in preterm labor and full term labor. Method: This is a cross sectional study with 68 samples, 29 subjects with preterm labor and 39 subjects with full term labor. IL-8 concentration was obtained from blood samples of the subjects, which were examined at Prodia Laboratory Denpasar. Data was analyzed using t-test for independent samples with =0.05. Result: The mean IL-8 level for the preterm labor group was 23.56 10.69 pg/ml and 12.19 5.79 pg/ml for the full term labor group. Statistical analysis using independent samples t-test showed that the average IL-8 level of both groups were significantly different (p=0.001). Conclusion: We concluded from this study that serum IL-8 concentration in women who had preterm labor is significantly higher in comparison to women who had full term labor. [Indones J Obstet Gynecol 2014; 4: 185-187] Keywords: full term labor, interleukin-8, preterm labor

scholarly journals TRPC3 Overexpression Promotes the Progression of Inflammation-Induced Preterm Labor and Inhibits T Cell Activation

2018 ◽  
Vol 45 (1) ◽  
pp. 378-388 ◽  
Author(s):  
Chen Jing ◽  
Zheng Dongming ◽  
Cui Hong ◽  
Na Quan ◽  
Liu Sishi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Peripheral Blood ◽  
Preterm Labor ◽  
Full Term ◽  
Wild Type ◽  
Uterine Tissue ◽  
Cd8 T Lymphocytes ◽  
Term Labor

Background/Aims: To detect the expression of the TRPC3 channel protein in the tissues of women experiencing preterm labor and investigate its interaction with T lymphocytes, providing a theoretical basis for the clinical prevention of threatened preterm labor and the development of drug-targeted therapy. Methods: Forty-seven women experiencing preterm labor and 47 women experiencing normal full-term labor were included in this study. All included women underwent delivery via cesarean section; uterine samples were obtained at delivery. The expression of TRPC3 in uterine tissue was detected by immunohistochemistry, real-time quantitative reverse transcription-PCR, and western blot assay. Activation of T lymphocytes in peripheral blood and uterine tissue were detected by flow cytometry. A TRPC3-/- mouse model of inflammation-induced preterm labor was established; expression of TRPC3, Cav3.1, and Cav3.2 were analyzed in mouse uterine tissue. Activation of T lymphocytes in female mouse and human peripheral blood samples was determined using flow cytometry. Results: In women experiencing preterm labor, expression of TRPC3 and the Cav3.1 and Cav3.2 proteins was significantly increased; in addition, the percentage of CD3+, CD4+, and CD8+ T cells in peripheral blood was significantly decreased. TRPC3 knockout significantly delayed the occurrence of preterm labor in mice. The muscle tension of ex vivo uterine strips was lower, Cav3.1 and Cav3.2 protein expression was lower, and the percentage of CD8+ T lymphocytes was significantly increased in wild-type mice subjected to an inflammation-induced preterm labor than in wild-type mice experiencing normal full-term labor. Conclusion: TRPC3 is closely related to the initiation of labor. TRPC3 relies on Cav3.1 and Cav3.2 proteins to inhibit inflammation-induced preterm labor by inhibiting the activation of T cells, in particular CD8+ T lymphocytes.

scholarly journals Corticotropin-Releasing Hormone Receptor Subtype 1 Is Significantly Up-Regulated at the Time of Labor in the Human Myometrium1

1998 ◽  
Vol 83 (11) ◽  
pp. 4107-4115
Author(s):  
M. Yvette Stevens ◽  
John R. G. Challis ◽  
Stephen J. Lye
Keyword(s):  
Human Myometrium ◽  
Receptor Subtype ◽  
Postpartum Women ◽  
Mediating Effects ◽  
Rt Pcr ◽  
Lower Segment ◽  
Uterine Activity ◽  
Messenger Ribonucleic Acid ◽  
Crh Receptors ◽  
Term Labor

Circulating concentrations of CRH rise late in human pregnancy, reaching a peak at labor. The presence of functional CRH receptors, CRH-R1 and CRH-R2, in the human myometrium suggests that CRH may modulate uterine activity. We hypothesized that the number of CRH receptors would be higher in myometrium than fetal membranes (FM) and would change during labor. Myometrial samples were collected from the lower segment (LS) in nonpregnant, preterm (32 ± 2 weeks), and term (39 ± 1.6 weeks) pregnant patients before and at labor. Fundus and LS samples were also collected from nonpregnant, pregnant, laboring, and postpartum women. FM were collected at term and at labor. We identified CRH receptors in myometrium and FM by semiquantitative RT-PCR and immunohistochemistry. CRH-R1 messenger ribonucleic acid (mRNA) in the LS was decreased in pregnancy and increased significantly in both preterm and term labor (P < 0.05), but remained unchanged in the fundus. CRH-R2 mRNA was present in 28% of LS myometrium with no change at labor. CRH-R1 and CRH-R2 protein was localized to myometrial smooth muscle in nonpregnant and laboring patients, with lower levels at term. CRH-R1 mRNA was present in chorion and decidua, but CRH-R2 was undetectable in these tissues. We conclude that CRH-R1 is expressed preferentially in myometrium and FM. Changes in CRH receptors during labor are consistent with CRH mediating effects on myometrial activity.

scholarly journals Role of nuclear factor kappa beta, tumor necrosis factor α, and cyclooxygenase-2 in preterm labor

2015 ◽  
Vol 23 (4) ◽  
pp. 213-7
Author(s):  
Syamsul B. Riva'i ◽  
Eryati Darwin ◽  
Ellyza Nasrul ◽  
Jusuf S. Effendi
Keyword(s):  
Tumor Necrosis Factor ◽  
Preterm Labor ◽  
Tumor Necrosis ◽  
Cyclooxygenase 2 ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Nuclear Factor Kappa Beta ◽  
Cox 2 ◽  
Term Labor ◽  
Necrosis Factor

Background: The pathway of tumor necrosis factor alpha (TNFα), nuclear factor kappa beta (NF-κB), and cyclooxygenase-2 (COX-2) activation in releasing prostaglandins is suggested to be crucial for initiating labor in the pathogenesis of preterm labor. The aim of the study was to know whether there were mean differences of NF-κB, TNFα, and COX-2 expressions between preterm and term labor and also to know the correlation among them in preterm labor.Methods: A case-control study was performed from May 2013 to February 2014 in Arifin Achmad Hospital, Pekanbaru. There were 30 subjects with preterm labor as cases and 30 with normal labor as controls. All subjects had singleton gestation with maximum parity was three, age limit of 35 year-old, and spontaneous labor in both groups. Placental tissue was collected from all subjects and evaluated with hematoxylin eosin staining. The expressions of TNFα, NF-κB, and COX-2 in the tissue were assessed with immunohistochemical staining by counting the percentage of smeared cells by two experts. The expressions of TNFα, NF-κB, and COX-2 between case and control were compared using t-test and the correlation was analyzed with Pearson correlation coefficient.Results: Mean (SD) of expressions of TNFα (93.05% [12.68] vs 49.11% [27.33]), NF-κB (42.46% [27.29] vs 13.66% [17.77]), and COX-2 (88.75% [10.86] vs 46% [30.36]) were significantly higher in the preterm labor compared to term labor (p = 0.001). There was significant correlation between TNFα and NF-κB expression (r = 0.385; p = 0.036) and no correlation was found between NF-κB and COX-2 (p = 0.982) in preterm labor.Conclusion: High expressions of TNFα, NF-κB, and COX-2 in preterm labor showed to contribute in the onset of preterm labor. High TNFα may suggest that infection was a leading cause of preterm labor. This is supported with an increase in NF-κB activation will increase COX-2 and subsequently prostaglandins that result in premature labor.

Chemotactic Activity of Gestational Tissues Through Late Pregnancy, Term Labor, and RU486-Induced Preterm Labor in Guinea Pigs

2014 ◽  
Vol 73 (4) ◽  
pp. 341-352 ◽  
Author(s):  
Nardhy Gomez-Lopez ◽  
Wing-Chiu Tong ◽  
Marcia Arenas-Hernandez ◽  
Satomi Tanaka ◽  
Omar Hajar ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Preterm Labor ◽  
Late Pregnancy ◽  
Chemotactic Activity ◽  
Gestational Tissues ◽  
Term Labor

Uterine contraction and physiological mechanisms of modulation

1993 ◽  
Vol 264 (1) ◽  
pp. C1-C18 ◽  
Author(s):  
S. Wray
Keyword(s):  
Smooth Muscle ◽  
Preterm Labor ◽  
Uterine Contraction ◽  
Contractile Activity ◽  
Detailed Account ◽  
Physiological Mechanisms ◽  
Vital Importance ◽  
Clinical Problems ◽  
Term Labor

Control of the smooth muscle in the uterus (the myometrium), is of vital importance during pregnancy and parturition. It is therefore understandable that several physiological mechanisms (neuronal, hormonal, metabolic, and mechanical) play a role in the control of myometrial activity. As our knowledge of the mechanism of uterine contraction has increased much in recent years, it is now possible to begin to explain, in some detail, how the contractile activity may be modulated. A detailed account of the mechanism of contraction in the uterus is therefore given, followed by examples of modulation of this process for each of the four physiological methods listed above. Examples have been chosen to illustrate either general or particular mechanisms of modulation. The goal of many working in this field is to understand these processes and thus prevent preterm labor and uterine dysfunction in term labor, which are still significant clinical problems.

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