scholarly journals DNA damage by Withanone as a potential cause of liver toxicity observed for herbal products of Withania somnifera (Ashwagandha)

2021 ◽  
Vol 2 ◽  
pp. 72-81
Author(s):  
Shazia Siddiqui ◽  
Nabeel Ahmed ◽  
Mausumi Goswami ◽  
Anindita Chakrabarty ◽  
Goutam Chowdhury
Keyword(s):  
Dna Damage ◽  
Withania Somnifera ◽  
Liver Toxicity ◽  
Herbal Products

scholarly journals Roles of Moringa oleifera Leaf Extract in Improving the Impact of High Dietary Intake of Monosodium Glutamate-Induced Liver Toxicity, Oxidative Stress, Genotoxicity, DNA Damage, and PCNA Alterations in Male Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Tarfa Albrahim ◽  
Manal Abdulaziz Binobead
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Leaf Extract ◽  
Liver Toxicity ◽  
Monosodium Glutamate ◽  
Male Rats ◽  
Control Group ◽  
Gamma Glutamyl Transferase ◽  
Glutamyl Transferase ◽  
The Impact

It is common for food to be made more palatable through the use of the flavour enhancer monosodium glutamate, also known as vetsin powder. The purpose of the study described in this paper was to explore how vetsin-induced hepatic toxicity, DNA fragmentation, damage, and oxidative stress modifications could be mitigated with moringa leaf extract (MLE). To that end, 40 male rats were separated into four groups: normal control, positive control or MLE, vetsin, and vetsin combined with MLE. Results indicated that, compared to the control group, the levels of serum alanine aminotransferase (ALT), aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), liver malondialdehyde (MDA), DNA damage, injury, PCNA, and P53 expressions were significantly enhanced by the administration of vetsin (P<0.05). However, the vetsin group had significantly reduced levels of albumin, globulin, total protein, liver glutathione (GSH), superoxide dismutase enzyme (SOD), catalase, and glutathione S-transferase (GST) enzyme activities (P<0.05) by comparison to control. Meanwhile, modifications in liver functions, oxidative stress, DNA damage, liver injury, and PCNA expression were alleviated when vetsin was administered alongside MLE. The authors conclude that vetsin may have many side effects and that MLE can ameliorate biochemical changes, oxidative stress, hepatic injury, PCNA, and P53 alterations induced by vetsin administration.

scholarly journals Hepatoprotective Screening of Seriphidium kurramense (Qazilb.) Y.R. Ling

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Maroof Ali ◽  
Hidayat Hussain ◽  
Amjad Hussain ◽  
Abdur Rauf ◽  
Wahid Hussain ◽  
...  
Keyword(s):  
Dna Damage ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Nitrate Content ◽  
Serum Samples ◽  
Morphological Alterations ◽  
Ccl4 Administration ◽  
Altered Liver

Investigation on medicinal plants’ therapeutic potential has gained substantial importance in the discovery of novel effective and safe therapeutic agents. The present study is aimed at investigating the hepatoprotective potential of Seriphidium kurramense methanolic extract (SKM) against carbon tetrachloride- (CCl4-) induced hepatotoxicity in rats. S. kurramense is one of the most imperative plants for its various pharmacological activities. Therefore, this study was aimed at evaluating the hepatoprotective potential against CCl4-induced liver toxicity. The serum samples were analyzed for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) together with the oxidative stress mediator levels as nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), reduced glutathione (GSH), and superoxide dismutase (SOD) as well as peroxidation and H2O2 activity. CCl4 administration resulted in an elevated free radical generation, altered liver marker (AST and ALT) enzymes, reduced antioxidant enzyme, and increased DNA damage. Methanolic extract of S. kurramense decreased CCl4-induced hepatotoxicity by increasing the antioxidant status and reducing H2O2 and nitrate content generation as well as reducing DNA damage. Additionally, SKM reversed the morphological alterations induced by CCl4 in the SKM-treated groups. These results demonstrated that SKM displayed hepatoprotective activity against CCl4-induced hepatic damage in experimental rats.

scholarly journals Expression of iron metabolism genes as markers of organ toxicity

1970 ◽  
pp. 286-292
Author(s):  
I. P. Kindrat ◽  
H. M. Erstenyuk
Keyword(s):  
Dna Damage ◽  
Iron Metabolism ◽  
Iron Homeostasis ◽  
Liver Toxicity ◽  
Organ Injury ◽  
Marker Genes ◽  
Metabolic Disturbances ◽  
Fischer 344 ◽  
Reverse Transcription Pcr ◽  
Organ Specific

Aim. An increasing number of toxicants in the environment causes harmful effects on organism, resulting in broad range of metabolic disturbances, including iron metabolism. Perturbations in iron homeostasis may lead to the development of various pathological states, including organ injury and carcinogenesis. In this study, we investigated the effect of liver toxicant, bis-(2-ethylhexyl) phtalete (DEHP), and kidney toxicant, aristolochic acid (AA), on tissue-specific iron metabolism in rats. Methods. Gene expression in the livers and kidneys of Fischer 344 rats was determined by quantitative reverse transcription-PCR. Results. DEHP treatment increased the expression of liver toxicity and DNA damage marker genes, and iron-related genes, Ftl1, Fth1, Slc40a1, and decrease the expression of miR-122 and Hamp in the livers, but not in the kidneys. In contrast, AA increased the expression of kidney toxicity and DNA damage markers, and iron homeostasis genes, Ftl1, Fth1, Slc40a1 in the kidneys. Conclusions. Our results indicate an existence of organ-specific changes in the expression of iron metabolism genes in rats treated with DEHP and AA, respectively. These changes were accompanied by increasing of DNA damage and toxicity markers in the liver of DEHP-treated rats and in the kidneys of rats treated with AA. Keywords: toxicity, iron metabolism, liver, kidney.

scholarly journals Proximate Nutritive Values and Mineral Components ofWithania Somnifera(Linn.) Dunal

2010 ◽  
Vol 7 (3) ◽  
pp. 985-996 ◽  
Author(s):  
S. R. Krishnamurthy ◽  
P. Sarala
Keyword(s):  
Elemental Composition ◽  
Withania Somnifera ◽  
Nutritive Value ◽  
Sodium Potassium ◽  
Herbal Products ◽  
Geographical Regions ◽  
Mineral Components ◽  
Medicinal Properties ◽  
Different Parts

Withania somnifera(Linn.) Dunal is a subtropical shrub with important medicinal properties. The nutritive value and the elemental composition of different parts of plants,Withania somniferawhich are grown in two distinct geographical regions (Sondekola and Karthikere) of Karnataka have been determined. The investigation revealed that the variation of macro, micro and proximate components varied not only in the plants of different regions but also in the different parts of the same plant. Among the macro elements, Karthikere samples recorded maximum values of nitrogen, phosphorous and magnesium and Sondekola samples recorded maximum values of sodium, potassium and calcium. Among the components of micronutrients, the highest values of iron were recorded both in Sondekola and Karthikere samples. The average values of manganese, copper and zinc were more in the Karthikere samples and comparatively less in the Sondekola samples. Whereas, all the samples of Sondekola recorded maximum values of nutrition. It is believed that the dry climatic condition of the region may contribute the high values of nutrition. Further, the observations are discussed with reference to the geography, elemental composition and nutritional values. The strong and negative observations on herbal drugs and their validity, the study emphasizes the role of elemental composition, proximate components, nutritive value, habitat and geographical features which influence growth and development ofWithania somniferaand also herbal products ofWithania somniferain particular and medicinal plants in general.

scholarly journals Herbal Informatics: A Unique Model to Identify the Anti-Cancerous Agents for Targeting Lung Cancer

2020 ◽  
Author(s):  
Rashmi Wardhan ◽  
Ankit Tanwar ◽  
Pallavi Dutta ◽  
Ishita Jha ◽  
Ruby Sharma ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Azadirachta Indica ◽  
Withania Somnifera ◽  
Effective Management ◽  
Herbal Products ◽  
Piper Longum ◽  
Unique Model ◽  
Selection Of

The incidence of lung cancer has increased in recent years and causes major mortalities across the globe. Besides, the availability of the several chemotherapeutics modalities in the management, there is still a challenge to find out an efficient remedy with lesser or no toxic effects. Hence, there is a necessity to employ complementary research to establish effective management for lung cancer. In this study, we have implemented a novel herbal informatics model to find out the alternative remedy in the treatment of lung cancer. This model utilizes five major steps of the bioprospection process based on the classical surge followed by the binary index and rationale-based selection of herbal products targeting the cancer-causing factors which are explained in detail in the methodology section of this model. This study revealed 07 herbals such as Withania somnifera (Ws), Berberis vulgaris(Bv), Glycyrrhiza glabra(Gg), Andrographis paniculate(Ap), Azadirachta indica(Ai), Cinnamomum Verum(Cv), Piper longum(Pl) based on the fuzzy set optimization scoring(0.6-1) that could be further studied in vitro and in vivo level for utilization in the management of lung cancer.

Sign in / Sign up

Export Citation Format

Share Document