Purification of novel bacteriocin produced by Lactobacillus coryniformis MXJ 32 for inhibiting bacterial foodborne pathogens including antibiotic-resistant microorganisms

This study was designed to evaluate the effects of bile acid deconjugation by probiotic strains on the antibiotic susceptibility of antibiotic-sensitive and multiple antibiotic–resistant Salmonella Typhimurium and Staphylococcus aureus. Eight probiotic strains, Bifidobacterium longum B6, Lactobacillus acidophilus ADH, Lactobacillus brevis KACC 10553, Lactobacillus casei KACC 12413, Lactobacillus paracasei ATCC 25598, Lactobacillus rhamnosus GG, Leuconostoc mesenteroides KACC 12312, and Pediococcus acidilactici KACC 12307, were used to examine bile acid tolerance. The ability to deconjugate bile acids was evaluated using both thin-layer chromatography and high-performance liquid chromatography. The antibiotic susceptibility testing was carried out to determine the synergistic inhibitory activity of deconjugated bile acids. L. acidophilus, L. brevis, and P. acidilactici showed the most tolerance to the conjugated bile acids. P. acidilactici deconjugated glycocholic acid and glycodeoxycholate from 3.18 and 3.09 mM to the detection limits, respectively. The antibiotic susceptibility of selected foodborne pathogens was increased by increasing the concentration of deconjugated bile acids. The study results are useful for understanding the relationship between bile acid deconjugation by probiotic strains and antibiotic susceptibility in the presence of deconjugated bile acids, and they may be useful for designing new probiotic-antibiotic combination therapy based on bile acid deconjugation.

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Prevalence of Multidrug-Resistant Foodborne Pathogens and Indicator Bacteria from Edible Offal and Muscle Meats in Nashville, Tennessee

Foods ◽

10.3390/foods9091190 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1190

Author(s):

Siqin Liu ◽

Agnes Kilonzo-Nthenge ◽

Samuel N. Nahashon ◽

Bharat Pokharel ◽

Abdullah Ibn Mafiz ◽

...

Keyword(s):

Foodborne Pathogens ◽

Salmonella Enterica ◽

Ground Beef ◽

Multidrug Resistant ◽

Indicator Bacteria ◽

Resistant Bacteria ◽

Antibiotic Resistant ◽

Beef Liver ◽

E Coli ◽

Retail Meats

This study investigated the prevalence of antimicrobial-resistant bacteria in retail edible offal and muscle meats in Nashville, Tennessee. A total of 348 retail meats (160 edible offal and 188 muscle) were analyzed for Salmonella enterica serovar, Campylobacter, Escherichia coli, E. coli O157:H7, and enterococci. Bacteria was identified using biochemical and PCR methods. Salmonella enterica serovar (4.4% and 4.3%), Campylobacter (1.9% and 1.1%), E. coli (79.4% and 89.4%), and enterococci (88.1% and 95.7%) was detected in offal and muscle meats, respectively. Chicken liver (9.7%) was most frequently contaminated with Salmonella enterica serovar, followed by ground chicken (6.9%) and chicken wings (4.2%). No Salmonella enterica serovar was detected in beef liver, beef tripe, and ground beef. The prevalence of Campylobacter was 6.9%, 2.3%, and 1.4% in beef liver, ground beef, and ground chicken, respectively. None of the meats were positive for E. coli O157:H7. Resistance of isolates was significantly (p < 0.05) highest in erythromycin (98.3%; 99.1%), followed by tetracycline (94%; 98.3%), vancomycin (88.8%; 92.2%) as compared to chloramphenicol (43.1%; 53.9%), amoxicillin/clavulanic (43.5%; 45.7%), and ciprofloxacin (45.7%; 55.7%) in offal and muscle meats, respectively. Imipenem showed the lowest resistance (0%; 0.9%). A total of 41 multidrug-resistant patterns were displayed. Edible offal could be a source of antibiotic-resistant bacteria.

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Susceptibility of Antibiotic-Resistant and Antibiotic-Sensitive Foodborne Pathogens to Acid Anionic Sanitizers

Journal of Food Protection ◽

10.4315/0362-028x-61.10.1390 ◽

1998 ◽

Vol 61 (10) ◽

pp. 1390-1395 ◽

Cited By ~ 9

Author(s):

JOHN A. LOPES

Keyword(s):

Foodborne Pathogens ◽

Fruits And Vegetables ◽

Food Additives ◽

Attractive Alternative ◽

Gram Negative Bacteria ◽

Antibiotic Resistant ◽

Microbicidal Activity ◽

E Coli ◽

Bactericidal Efficacy ◽

Resistant Strains

Acid anionic sanitizers for treatment of fruits and vegetables were prepared using ingredients generally recognized as safe by the U.S. Food and Drug Administration or anionic surfactants and organic acid food additives. They met the regulatory definition as sanitizers by showing bactericidal efficacy of 99.999% in 30 s against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 11229. These sanitizers showed a broad spectrum of microbicidal activity against both gram-positive and gram-negative bacteria. Antibiotic-sensitive and resistant strains of Listeria monocytogenes and Salmonella typhimurium were equally susceptible to these sanitizers. The acid anionic sanitizers showed microbicidal efficacy equal to that of hypochlorite against Aeromonas hydrophila, E. coli O157:H7, L. monocytogenes, Pseudomonas aeruginosa, S. typhimurium, and S. aureus. Unlike most other sanitizers, these agents do not covalently react with organic components of food; unlike cationic agents, they do not leave residues. The acid anionic sanitizers are prepared using stable, biodegradable, and nontoxic ingredients. Rapid microbicidal activity and the ease of storage, transportation, and use make these sanitizers an attractive alternative to hypochlorite for sanitizing fruits and vegetables.

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Antimicrobial properties of rosin acids-loaded nanoparticles against antibiotic-sensitive and antibiotic-resistant foodborne pathogens

Artificial Cells Nanomedicine and Biotechnology ◽

10.1080/21691401.2018.1496924 ◽

2018 ◽

Vol 46 (sup3) ◽

pp. S414-S422 ◽

Cited By ~ 5

Author(s):

Elisa Santovito ◽

José das Neves ◽

Donato Greco ◽

Vito D’Ascanio ◽

Bruno Sarmento ◽

...

Keyword(s):

Foodborne Pathogens ◽

Antimicrobial Properties ◽

Antibiotic Resistant ◽

Rosin Acids

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Lactic acid bacterial bacteriocins and their bioactive properties against food-associated antibiotic-resistant bacteria

Annals of Microbiology ◽

10.1186/s13213-021-01652-6 ◽

2021 ◽

Vol 71 (1) ◽

Author(s):

Emmanuel Edoghogho Imade ◽

Solomon Esharegoma Omonigho ◽

Olubukola Oluranti Babalola ◽

Ben Jesuorsemwen Enagbonma

Keyword(s):

Lactic Acid ◽

Foodborne Pathogens ◽

Food Products ◽

Lactobacillus Fermentum ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

E Coli ◽

Agar Well Diffusion Assay ◽

Diffusion Assay

Abstract Purpose Incidence of foodborne diseases and growing resistance of pathogens to classical antibiotics is a major concern in the food industry. Consequently, there is increasing demand for safe foods with fewer chemical additives but natural products which are not harmful to the consumers. Bacteriocins, produced by lactic acid bacteria (LAB), is of interest because they are active in a nanomolar range, do not have toxic effects, and are readily available in fermented food products. Methods In this research, LAB were isolated from fufu, gari, kunu, nono, and ogi using De Mann, Rogosa, and Sharpe agar. Cell-free supernatants were prepared from 18-24 h LAB culture grown on MRS broth. Effect of organic acid was eliminated by adjusting the pH of the supernatants to 7.0 with 1M NaOH while the effect of hydrogen peroxide was eliminated by treating with Catalase enzyme. The supernatant was then filter-sterilized using a membrane filtration unit with a 0.2-μm pore size millipore filter and subjected to agar well diffusion assay against foodborne antibiotic-resistant bacteria. Result A total of 162 isolates were obtained from the food samples. The antimicrobial sensitivity test yielded positive results for 45 LAB isolates against Staphylococcus aureus ATCC 25923 while 52 LAB isolates inhibited Escherichia coli ATCC 25922. On confirmation of the bacteriocinogenic nature of the inhibitory substance, 4 of the LAB isolates displayed a remarkable degree of inhibition to Leuconostoc mesenteroides, Salmonella typhimurium, and Bacillus cereus. Agar well diffusion assay was also performed against antibiotic-resistant foodborne pathogens using the cell-free supernatant (CFS) obtained from Lactobacillus fermentum strain NBRC15885 (Limosilactobacillus fermentum), Lactobacillus fermentum strain CIP102980 (Limosilactobacillus fermentum), Lactobacillus plantarum strain JCM1149 (Lactiplantibacillus garii), and Lactobacillus natensis strain LP33 (Companilactobacillus nantensis). The foodborne pathogens exhibited a notable level of resistance to antibiotics, with B. cereus exhibiting a resistance profile of 40%, S. aureus (50%), K. pnuemoniae (70%), E. coli (60%), and S. typhi (40%). The (CFS) was able to inhibit the growth of B. cereus, Klebsiella pneumonia, S. typhimurium, S. aureus, and E. coli. Conclusion Therefore, it portends that the bacteriocins produced by the LAB isolated from these food products could act as probiotics for effective inhibition of the growth of antibiotic-resistant foodborne pathogens.

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Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia

Veterinary World ◽

10.14202/vetworld.2021.1767-1773 ◽

2021 ◽

pp. 1767-1773

Author(s):

María Paula Herrera-Sánchez ◽

Rafael Enrique Castro-Vargas ◽

Luz Clemencia Fandiño-de-Rubio ◽

Roy Rodríguez-Hernández ◽

Iang Schroniltgen Rondón-Barragán

Keyword(s):

Molecular Identification ◽

Foodborne Pathogens ◽

Fluoroquinolone Resistance ◽

Diffusion Method ◽

Salmonella Spp ◽

Class 1 Integron ◽

Antibiotic Resistant ◽

Automated Method ◽

Class 1 ◽

Resistant Strains

Background and Aim: Salmonella is one of the most common foodborne pathogens, the emergence of antibiotic-resistant strains of which is increasing. The aim of this study was to phenotypically and genotypically characterize the fluoroquinolone resistance of Salmonella isolates from broiler and humans in two regions of Colombia. Materials and Methods: Salmonella strains (n=49) were evaluated. The phenotype of antibiotic resistance was assessed by an automated method and agar diffusion method, as well as the presence of the quinolone resistance genes qnrA, qnrB, qnrC, qnrD, qnrS, and aac(6')-Ib as determined by polymerase chain reaction. Results: Strains were resistant to ciprofloxacin (75%), levofloxacin (57.1%), and enrofloxacin (38.8%). Molecular identification showed that 24 out of 49 strains possessed the qnrB gene (48.9%), while only one isolate from the Santander region possessed the aac(6')-Ib gene. Regarding Class 1 integron, it was present in 11 out of the 49 strains (22.44%). Conclusion: This study reports the presence of the gene qnrB as well the presence of Class 1 integrons in broiler Salmonella isolates, which may contribute to the resistance to fluoroquinolones.

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Understanding and Exploiting Phage–Host Interactions

Viruses ◽

10.3390/v11060567 ◽

2019 ◽

Vol 11 (6) ◽

pp. 567 ◽

Cited By ~ 11

Author(s):

Edel Stone ◽

Katrina Campbell ◽

Irene Grant ◽

Olivia McAuliffe

Keyword(s):

Foodborne Pathogens ◽

Bacterial Infections ◽

Food Industry ◽

Host Cells ◽

Clinical Settings ◽

Antibiotic Resistant ◽

Host Interactions ◽

Detection Systems ◽

The Relationship ◽

Biology Research

Initially described a century ago by William Twort and Felix d’Herelle, bacteriophages are bacterial viruses found ubiquitously in nature, located wherever their host cells are present. Translated literally, bacteriophage (phage) means ‘bacteria eater’. Phages interact and infect specific bacteria while not affecting other bacteria or cell lines of other organisms. Due to the specificity of these phage–host interactions, the relationship between phages and their host cells has been the topic of much research. The advances in phage biology research have led to the exploitation of these phage–host interactions and the application of phages in the agricultural and food industry. Phages may provide an alternative to the use of antibiotics, as it is well known that the emergence of antibiotic-resistant bacterial infections has become an epidemic in clinical settings. In agriculture, pre-harvest and/or post-harvest application of phages to crops may prevent the colonisation of bacteria that are detrimental to plant or human health. In addition, the abundance of data generated from genome sequencing has allowed the development of phage-derived bacterial detection systems of foodborne pathogens. This review aims to outline the specific interactions between phages and their host and how these interactions may be exploited and applied in the food industry.

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Twenty-year trends in antimicrobial resistance from aquaculture and fisheries in Asia

Nature Communications ◽

10.1038/s41467-021-25655-8 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Daniel Schar ◽

Cheng Zhao ◽

Yu Wang ◽

D. G. Joakim Larsson ◽

Marius Gilbert ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Foodborne Pathogens ◽

Meta Analysis ◽

Animal Health ◽

Eastern China ◽

Resistant Bacteria ◽

Antibiotic Resistant ◽

China And India ◽

Aquatic Food ◽

The Impact

AbstractAntimicrobial resistance (AMR) is a growing threat to human and animal health. However, in aquatic animals—the fastest growing food animal sector globally—AMR trends are seldom documented, particularly in Asia, which contributes two-thirds of global food fish production. Here, we present a systematic review and meta-analysis of 749 point prevalence surveys reporting antibiotic-resistant bacteria from aquatic food animals in Asia, extracted from 343 articles published in 2000–2019. We find concerning levels of resistance to medically important antimicrobials in foodborne pathogens. In aquaculture, the percentage of antimicrobial compounds per survey with resistance exceeding 50% (P50) plateaued at 33% [95% confidence interval (CI) 28 to 37%] between 2000 and 2018. In fisheries, P50 decreased from 52% [95% CI 39 to 65%] to 22% [95% CI 14 to 30%]. We map AMR at 10-kilometer resolution, finding resistance hotspots along Asia’s major river systems and coastal waters of China and India. Regions benefitting most from future surveillance efforts are eastern China and India. Scaling up surveillance to strengthen epidemiological evidence on AMR and inform aquaculture and fisheries interventions is needed to mitigate the impact of AMR globally.

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Analysis of Fish Commonly Sold in Local Supermarkets Reveals the Presence of Pathogenic and Multidrug-Resistant Bacterial Communities

Microbiology Insights ◽

10.1177/1178636118786925 ◽

2018 ◽

Vol 11 ◽

pp. 117863611878692 ◽

Cited By ~ 1

Author(s):

Ama Okyere ◽

Dayna Bishoff ◽

Micah O Oyaro ◽

Nadim J Ajami ◽

Charles Darkoh

Keyword(s):

Foodborne Pathogens ◽

Bacterial Communities ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Human Pathogens ◽

Culture Methods ◽

Antibiotic Resistant ◽

E Coli ◽

Fish Samples ◽

And Staphylococcus Aureus

Fish has been an important source of proteins, essential vitamins, and low saturated fats for centuries. However, improperly handled fish can expose consumers to infectious bacteria, including difficult to treat multidrug-resistant pathogens. With the goal to investigate the existence of disease-causing and antibiotic-resistant bacteria, we examined bacterial communities present on various types of fish purchased from supermarkets in Houston, Texas, USA. The bacterial communities were characterized by selective phenotypic culture methods, 16S ribosomal RNA gene sequencing, and antibiotic susceptibility testing. The results revealed the presence of different bacterial communities on the fish samples examined. The bacterial communities were not significantly different between the supermarkets sampled. The following presumptive human pathogens were isolated on the fish samples: Escherichia coli (67%), enterohemorrhagic E. coli (31%), Shigella and Salmonella species (28%), Listeria species (29%), and Staphylococcus aureus (28%). Drug sensitivity assays showed resistance to commonly prescribed antibiotics ciprofloxacin, gentamicin, and vancomycin. Out of a total of 99 E. coli samples tested, 41.4% were resistant to ciprofloxacin, whereas 33.3% were resistant to gentamicin. Of the total of 31 S. aureus isolates tested, 87% were resistant to ciprofloxacin, whereas 61.3% were resistant to vancomycin. Moreover, some of the E. coli strains were resistant to both ciprofloxacin and gentamicin (28%), whereas 49% of the S. aureus isolates were resistant to both ciprofloxacin and vancomycin. These results highlight the prevalence of antimicrobial-resistant foodborne pathogens on fish purchased from the supermarkets and underscore the risk associated with improper handling of fish.

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Prevalence of antibiotic resistant pathogenic E. coli from animals, retail and humans diagnosed with Gastroenteritisv

Journal of Food Safety and Hygiene ◽

10.18502/jfsh.v6i2.6521 ◽

2021 ◽

Author(s):

Adriana M. Morales Gomez ◽

Elizabeth Aguilera Nunez ◽

Patrick McDonough ◽

Yung-Fu Chang ◽

Hussni O. Mohammed

Keyword(s):

Supply Chain ◽

Foodborne Pathogens ◽

Food Supply ◽

Phenotypic Expression ◽

Meat Processing ◽

Food Supply Chain ◽

Antibiotic Resistant ◽

E Coli ◽

Processing Plants ◽

Genetic Detection

Foodborne pathogens represent one of the major challenges to health systems around the world. This risk is exacerbated by the presence of antimicrobial resistant (AMR) pathogens. Knowledge of the presence of these pathogens in the food supply chain would help in establishing intervention strategies to mitigate their risk. The objective of this study was to detect AMR among serotypes of Escherichia coli (E. coli) food adulterants serotypes of E. coli in the food supply chain and among isolates from gastroenteritis cases. E. coli isolates recovered from animals, meat processing plants, retail, and humans were examined for the presence of AMR using phenotypic and genotypic approaches. AMR to aminoglycosides, β-lactams, and tetracycline were detected in all isolates recovered from these sources at different levels. Similarly, presence of the bla-Tem, bla-SHV, aadA, and strAB genes were detected in isolates from these sources but there was no significant correlation between the genetic detection and phenotypic expression AMR.

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