The possible role of catalase in innate immunity and diminution of cellular oxidative stress: Insights into its molecular characteristics, antioxidant activity, DNA protection, and transcriptional regulation in response to immune stimuli in yellowtail clownfish (Amphiprion clarkii)

2021 ◽  
Vol 113 ◽  
pp. 106-117
Author(s):  
W.M. Gayashani Sandamalika ◽  
Hyukjae Kwon ◽  
Chaehyeon Lim ◽  
Hyerim Yang ◽  
Jehee Lee
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Innate Immunity ◽  
Transcriptional Regulation ◽  
Molecular Characteristics ◽  
Dna Protection ◽  
Amphiprion Clarkii

In Vitro Study of Antioxidant Activity of Serum and Plasma Samples As Well As Glutathione Exposed to Various Exogenous Stress Factors

2021 ◽  
Author(s):  
Małgorzata Olszowy-Tomczyk ◽  
Łukasz Paprotny ◽  
Agnieszka Celejewska ◽  
Dorota Szewczak ◽  
Dorota Wianowska
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
In Vitro Study ◽  
Stress Factors ◽  
Plasma Samples ◽  
Serum Samples ◽  
Exogenous Stress

Abstract The imbalance between the production of Reactive Oxygen Species (ROS) and their sequestration promotes the formation of so-called oxidative stress conditions which are considered crucial in the aging process and development of many human diseases. Glutathione plays an essential role in the antioxidative barricade against ROS. Its role in the detoxification process of xenobiotics and carcinogen is also known. However, there are no comparative studies on the antioxidant properties of both biological samples and glutathione as well as the change in these properties as a result of exposure to various stress factors. This paper fills this gap comparing the antioxidant activity of serum and plasma samples of the known glutathione content with the activity of glutathione itself assessed by the different methods. In addition, it reveals a significant role of environmental xenobiotics in oxidative stress and differentiates the stress induced by different groups of drugs, among which the greatest one has been demonstrated for antiarrhythmic drugs and cytostatics. More importantly, it proves that human plasma is more resistant to stress factors and N-acetylcysteine clearly promotes the extension of antioxidant properties of both the plasma and serum samples. The latter conclusion is consistent with the implied preventive and/or supportive action of this drug against SARS-CoV-2.

The role of vitamin and mineral complexes in the elimination of oxidative stress and its consequences

2020 ◽  
pp. 58-66
Author(s):  
O. A. Gizinger
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Antioxidant Status ◽  
Multiple Organ ◽  
Adaptive Potential ◽  
Effective Prevention ◽  
Therapeutic Practice ◽  
Important Approach ◽  
Coronavirus Infection

A prolonged phase of self-isolation and hypodynamia caused by the coronavirus infection negatively affect most homeostatic reactions in the human body and antioxidant status. The use of vitamin and mineral complexes which contain substances with antioxidant activity are part of a comprehensive correction of antioxidant status and medication-based antioxidant therapy. This direction of general therapeutic practice marks an important approach to overcoming the combined multiple organ pathology, provides effective prevention of morbidity, and contributes to improving the adaptive potential of a person.

scholarly journals IMPACT OF ACTIVE COMPOUNDS ISOLATED FROM BANANA (MUSA SP. VAR. NANJANGUD RASABALE) FLOWER AND PSEUDOSTEM TOWARDS CYTOPROTECTIVE AND DNA PROTECTION ACTIVITIES

2017 ◽  
Vol 9 (10) ◽  
pp. 21
Author(s):  
Ramith Ramu ◽  
Prithvi S. Shirahatti ◽  
Shrisha Naik Bajpe ◽  
Varsha Reddy S V ◽  
M. N. Nagendra Prasad
Keyword(s):  
Oxidative Stress ◽  
Plasmid Dna ◽  
Ethanol Extract ◽  
Dna Protection ◽  
Active Compounds ◽  
Protective Properties ◽  
Musa Sp ◽  
Membrane Oxidation ◽  
Banana Flower

Objective: The present study was designed to evaluate for cytoprotective and DNA protective properties of the compounds isolated from ethanol extract of banana flower (EF) and ethanol extract of banana pseudostem (EE).Methods: The four active compounds viz., umbelliferone (C1) andlupeol (C2) from EF and stigmasterol (C3) and β-sitosterol (C4) from EE were isolated by activity-guided repeated fractionation through silica gel column chromatography. The isolated compounds were evaluated for cytoprotective on erythrocytes and pTZ57R/T plasmid DNA protection against hydroxyl radicals.Results: The study revealed that the compounds (C1-C4) at a concentration of 1 mg/ml exhibited 90% protection on erythrocytes membrane oxidation and also protect the pTZ57R/T plasmid DNA damage induced by hydroxyl radicals.Conclusion: These results endorse an insight for a strong chemical basis to the alleged beneficial role of EF and EE in reducing oxidative stress conditions. 

scholarly journals Hydrogen peroxide as a potential mediator of the transcriptional regulation of heparan sulphate biosynthesis in keratinocytes

2008 ◽  
Vol 13 (3) ◽  
Author(s):  
Fumiaki Nakayama ◽  
Akiko Hagiwara ◽  
Tetsuo Yamamoto ◽  
Makoto Akashi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Transcriptional Regulation ◽  
Heparan Sulfate ◽  
Heparan Sulphate ◽  
Transcript Level ◽  
Hacat Keratinocytes ◽  
Core Proteins ◽  
Radiation Induced

AbstractIonizing radiation is one of the types of oxidative stress that has a number of damaging effects on cutaneous tissues. One of the histological features of radiation-induced cutaneous fibrosis is the accumulation of extracellular matrix (ECM) components, including heparan sulfate proteoglycan (HSPG), which are required for the repair of tissue damage, and operate by interacting with a variety of growth factors. In this study, we established a model of human HaCaT keratinocytes overexpressing anti-oxidative enzyme genes to elucidate the mechanism of oxidative stress leading to the accumulation of HSPG and the role of its accumulation. Catalase overexpression induced an increase in anti-HS antibody (10E4) epitope expression in these cells. Western blotting showed that the smeared bands of HSPG were obviously shifted to a higher molecular weight in the catalase transfectants due to glycosylation. After heparitinase I treatment, the core proteins of HSPG were expressed in the catalase transfectants to almost the same extent as in the control cells. In addition, the transcript levels of all the enzymes required for the synthesis of the heparan sulfate chain were estimated in the catalase transfectant clones. The levels of five enzyme transcripts — xylosyltransferase-II (XT-II), EXTL2, D-glucuronyl C5-epimerase (GLCE), HS2-O-sulfotransferase (HS2ST), and HS6-O-sulfotransferase (HS6ST) — were significantly increased in the transfectants. Moreover, hydrogen peroxide was found to down-regulate the levels of these enzymes. By contrast, siRNA-mediated repression of catalase decreased 10E4 epitope expression, the transcript level of HS2ST1, and the growth rate of HaCaT cells. These findings suggested that peroxide-mediated transcriptional regulation of HS metabolism-related genes modified the HS chains in the HaCaT keratinocytes.

The role of vitamin and mineral complexes in the elimination of oxidative stress and its consequences

2020 ◽  
pp. 58-66
Author(s):  
O. A. Gizinger
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Antioxidant Status ◽  
Multiple Organ ◽  
Adaptive Potential ◽  
Effective Prevention ◽  
Therapeutic Practice ◽  
Important Approach ◽  
Coronavirus Infection

A prolonged phase of self-isolation and hypodynamia caused by the coronavirus infection negatively affect most homeostatic reactions in the human body and antioxidant status. The use of vitamin and mineral complexes which contain substances with antioxidant activity are part of a comprehensive correction of antioxidant status and medication-based antioxidant therapy. This direction of general therapeutic practice marks an important approach to overcoming the combined multiple organ pathology, provides effective prevention of morbidity, and contributes to improving the adaptive potential of a person.

Investigation of the role of α-lipoic acid on fatty acids profile, some minerals (zinc, copper, iron) and antioxidant activity against aluminum-induced oxidative stress in the liver of male rats

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Zafer Sahin ◽  
Ahmet Ozkaya ◽  
Okkes Yilmaz ◽  
Abdurrauf Yuce ◽  
Mehmet Gunes
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Antioxidant Activity ◽  
Lipoic Acid ◽  
Wistar Rats ◽  
Male Rats ◽  
Fatty Acids Profile ◽  
Male Wistar Rats ◽  
Aluminum Accumulation

AbstractBackground:We have investigated the effects of α-lipoic acid (LA), a powerful antioxidant, on the fatty acid (FA) profiles, aluminum accumulation, antioxidant activity and some minerals such as zinc, copper and iron against aluminum chloride (AlClMethods:Twenty-eight male Wistar rats were divided into four groups as control, LA, AlClResults:AlClConclusions:These results indicate that AlCl

scholarly journals THE EFFECT OF THE STEM ROT AT COMPOSITION AND CONTENT OF PHENOLIC COMPOUNDS IN LEAVES OF BIRCH (BETULA PENDULA ROTH.)

2019 ◽  
pp. 169-176
Author(s):  
Yevgeniy Vladimirovich Koltunov
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Biochemical Composition ◽  
Betula Pendula ◽  
Stem Rot ◽  
Economic Damage ◽  
Biochemical Processes ◽  
Physiological And Biochemical

Stem rot cause significant ecological and economic damage to forests. Affected stands are prone to windbreaks and windfalls. Value of wood and stability of stands are reduced. Despite intensive studies of resistance biochemical aspects to rotting diseases, role of phenolic compounds remains insufficiently clarified. The purpose of research was studying composition of phenolic compounds in birch leaves with a significant level of damage to the diameter of the trunk. Stem rot was determined by taking core from trunk. Biochemical composition of leaves determined by HPLC. There are 64 phenolic compounds was revealed. 24 factions identified, 45.83% of compounds in affected trees, content was increased. Flavonoids are prevail (63.64%). In 45.83% of compounds, content decreased. They had predominance of flavonoids (81.82%). All compounds possess antioxidant activity. They contain phenolglycosides, determining entomoresistance (arbutin, salicin, salidroside). The results showed that with significant infestation with rot, there is an increase in the content of phenolic compounds in the leaves, therefore, the biochemical processes of combating oxidative stress are activated. This is due to violation of physiological and biochemical processes in diseased trees.

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