Terpene synthase gene expression evaluation through RT-qPCR in Thymus caespititius

Equine genital squamous cell carcinomas (egSCCs) are among the most common equine tumors after sarcoids, severely impairing animal health and welfare. Equus caballus papillomavirus type 2 (EcPV2) infection is often related to these tumors. The aim of this study was to clarify the molecular mechanisms behind egSCCs associated with EcPV2 infection, investigating receptor activator of nuclear factor-kappa B ligand (RANKL) signaling in NF-kB pathway, together with the Wnt and IL17 signaling pathways. We analyzed the innate immune response through gene expression evaluation of key cytokines and transcription factors. Moreover, Ki67 index was assessed with immunohistochemistry. EcPV2-E6 DNA was checked, and viral presence was confirmed in 21 positive out to 23 cases (91%). Oncogene expression was confirmed in 14 cases (60.8%) for E6 and in 8 (34.7%) for E2. RANKL, nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB)-p50, NFKBp65, interleukin (IL)-6, IL17, IL23p19, IL8, IL12p35, IL12p40, β-catenin (BCATN1), FOS like 1 (FOSL1), and lymphoid enhancer binding factor 1 (LEF1) showed a significant upregulation in tumor samples compared to healthy tissues. Our results describe an inflammatory environment characterized by the activation of RANKL/RANK and IL17 with the relative downstream pathways, and a positive modulation of inflammatory cytokines genes such as IL6 and IL8. Moreover, the increase of BCATN1, FOSL1, and LEF1 gene expression suggests an activation of both canonical and non-canonical Wnt signaling pathway that could be critical for carcinogenesis and tumor progression.

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Role of the amygdalo-hippocampal transition area in the fear expression: evaluation by behavior and immediate early gene expression

Neuroscience ◽

10.1016/j.neuroscience.2003.11.022 ◽

2004 ◽

Vol 124 (1) ◽

pp. 247-260 ◽

Cited By ~ 11

Author(s):

M Fujisaki ◽

K Hashimoto ◽

M Iyo ◽

T Chiba

Keyword(s):

Gene Expression ◽

Immediate Early Gene ◽

Early Gene ◽

Immediate Early ◽

Early Gene Expression ◽

Transition Area ◽

Expression Evaluation ◽

Fear Expression

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Epidermal growth factor receptor gene expression evaluation in colorectal cancer patients

Indian Journal of Cancer ◽

10.4103/0019-509x.146771 ◽

2014 ◽

Vol 51 (3) ◽

pp. 358 ◽

Cited By ~ 3

Author(s):

AYegane Moghadam ◽

M Mazaheri ◽

M Jahantigh ◽

K Sabagh ◽

N Sanadgol ◽

...

Keyword(s):

Gene Expression ◽

Colorectal Cancer ◽

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Receptor Gene ◽

Growth Factor Receptor ◽

Epidermal Growth ◽

Colorectal Cancer Patients ◽

Expression Evaluation ◽

Receptor Gene Expression

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Differential gene expression associated with a floral scent polymorphism in the evening primrose Oenothera harringtonii (Onagraceae)

10.1101/2021.01.12.426409 ◽

2021 ◽

Author(s):

Lindsey L. Bechen ◽

Matthew G. Johnson ◽

Geoffrey T. Broadhead ◽

Rachel A. Levin ◽

Rick P. Overson ◽

...

Keyword(s):

Gene Expression ◽

Differential Gene Expression ◽

Floral Scent ◽

Terpene Synthase ◽

Rna Seq ◽

Loss Of Function ◽

Terpene Biosynthesis ◽

Terpene Synthases ◽

Evening Primrose ◽

Differential Gene

AbstractBackgroundPlant volatiles play an important role in both plant-pollinator and plant-herbivore interactions. Intraspecific polymorphisms in volatile production are ubiquitous, but studies that explore underlying differential gene expression are rare. Oenothera harringtonii populations are polymorphic in floral emission of the monoterpene (R)-(-)-linalool; some plants emit (R)-(-)-linalool (linalool+ plants) while others do not (linalool-plants). However, the genes associated with differential production of this floral volatile in Oenothera are unknown. We used RNA-Seq to broadly characterize differential gene expression involved in (R)-(-)-linalool biosynthesis. To identify genes that may be associated with the polymorphism for this trait, we used RNA-Seq to compare gene expression in six different Oenothera harringtonii tissues from each of three linalool+ and linalool-plants.ResultsThree clusters of differentially expressed genes were enriched for terpene synthase activity: two were characterized by tissue-specific upregulation and one by upregulation only in plants with flowers that produce (R)-(-)-linalool. A molecular phylogeny of all terpene synthases identified two putative (R)-(-)-linalool synthase transcripts in Oenothera harringtonii, a single allele of which is found exclusively in linalool+ plants.ConclusionsBy using a naturally occurring polymorphism and comparing different tissues, we were able to identify genes putatively involved in the biosynthesis of (R)-(-)-linalool. Expression of these genes in linalool-plants suggests a regulatory polymorphism, rather than a population-specific loss-of-function allele. Additional terpene biosynthesis-related genes that are up-regulated in plants that emit (R)-(-)-linalool may be associated with herbivore defense, suggesting a potential economy of scale between plant reproduction and defense.

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Changes in Gene Expression and Metabolite Profiles in Platanus acerifolia Leaves in Response to Feeding Damage Caused by Corythucha ciliata

International Journal of Molecular Sciences ◽

10.3390/ijms20143465 ◽

2019 ◽

Vol 20 (14) ◽

pp. 3465

Author(s):

Fengqi Li ◽

Chunyan Wu ◽

Youssef Dewer ◽

Du Li ◽

Cheng Qu ◽

...

Keyword(s):

Gene Expression ◽

Acid Metabolism ◽

Insect Pest ◽

Invasive Pest ◽

Terpene Synthase ◽

Pathway Enrichment Analysis ◽

Feeding Damage ◽

Alpha Linolenic Acid ◽

Platanus Acerifolia ◽

Metabolite Profiles

The sycamore lace bug, Corythucha ciliata (Say) is a highly invasive pest insect that feeds on sycamore trees (Platanus spp.) worldwide. The interaction between Platanus species and this insect pest has not yet been studied at the molecular level. Therefore, a recent study was conducted to compare the gene expression and metabolite profiles of Platanus acerifolia leaves in response to C. ciliata feeding damage after 24 and 48 h. We employed high throughput RNA sequencing (RNA- seq) to identify a total of 2,828 significantly differentially expressed genes (DEGs) after C. ciliata feeding. In addition, 303 unigenes were found to be up-regulated at both time points. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that monoterpenoid biosynthesis, the linoleic acid metabolism pathway, and alpha- linolenic acid metabolism were the most prominent pathways among the DEGs. Further analysis of the metabolite profiles showed that nine metabolites were significantly different before and after C. ciliata damage. In addition, we analyzed DEGs detected in the P. acerifolia and C. ciliata interaction using Mapman. The terpene synthase gene family was also identified. We suggest that the results obtained from DEGs and metabolite analysis can provide important information for the identification of genes involved in the P. acerifolia–C. ciliata interaction, which might be necessary for controlling C. ciliata efficiently.

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Metatranscriptomic approach for microbiome characterization and host gene expression evaluation for “Hoja de malvón” disease in Vitis vinifera cv. Malbec

10.21203/rs.3.rs-23526/v1 ◽

2020 ◽

Author(s):

Marcos Paolinelli ◽

Georgina Escoriaza ◽

Cecilia Cesari ◽

Sandra Garcia-Lampasona ◽

Rufina Hernandez-Martinez

Keyword(s):

Gene Expression ◽

Disease Process ◽

Departure Point ◽

Microbiome Composition ◽

Grapevine Trunk Diseases ◽

Advanced Stages ◽

Trunk Diseases ◽

Expression Evaluation ◽

Further Development

Abstract Background: Grapevine Trunk Diseases (GTD) threat worldwide wine and table grape production, mainly reducing grape yields and in advanced stages causing the death of diseased vines. A particular GTD of enormous concern in Argentinean vitiviniculture is the complex etiology disease locally known as "Hoja de malvón" (HDM). At least four different fungi are involved in the disease, which complicates the diagnostic and the design of strategies for vineyard management. Similar to Esca grape disease, factors that make this disease difficult to control are the presence of pathogens that not always correlates with disease symptoms or physiological changes in the host. Also the abiotic stress on grapevine seems to favors the disease process. Based on this background, it is essential to have molecular tools that allow for simultaneous explorations of the host immunity status and the microbiome composition. Results: A metatranscriptomic approach was followed and different strategies for microbiome characterization, throughmolecular marker reconstruction or unique kmer counts, were evaluated. Malbec microbiome was mainly represented for Dothideomycetes and Actinobacteria. Higher Basidiomycota/Ascomycota ratio was found in symptomatic (SYM) than in asymptomatic (ASYM) plants, with the Basidiomycota Arrambaria destruens found in higher levels in SYM plants. Besides, from mRNA-derived reads, the host functional status and main microbial functions based on gene expression were evaluated. Stress-tolerance mechanisms were activated on ASYM plants, being spermidine synthesis one of the most important, while from the microbiome side, prokaryotic tricarboxylic acid pathways were the primary differential function in SYM plants. Conclusions: This is a pioneering work for the characterization of multi-kingdom endophytic microbiome in woody tissues of grapevine cv. Malbec. Several microorganisms with negative interaction with GTD pathogens were identified and can be further explored as biological control agents for HDM disease. The integral analysis employed suggests that measuring Basidiomycota/Ascomycota ratio and spermidine-associated gene expression would help to monitor the sanitary status of grapevine and the propensity to develop HDM disease. This study provides a departure point for a better comprehension of HDM disease that can be used as a roadmap for further development of curative practices of GTD.

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Lycopene supplementation modulates plasma concentrations and epididymal adipose tissue mRNA of leptin, resistin andIL-6in diet-induced obese rats

British Journal Of Nutrition ◽

10.1017/s0007114513001256 ◽

2013 ◽

Vol 110 (10) ◽

pp. 1803-1809 ◽

Cited By ~ 47

Author(s):

Renata de Azevedo Melo Luvizotto ◽

Andre F. Nascimento ◽

Erika Imaizumi ◽

Damiana T. Pierine ◽

Sandro J. Conde ◽

...

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Control Diet ◽

Plasma Concentrations ◽

Epididymal Adipose Tissue ◽

Low Grade ◽

Monocyte Chemoattractant Protein 1 ◽

Male Wistar Rats ◽

Expression Evaluation

Obesity is characterised by chronic low-grade inflammation, and lycopene has been reported to display anti-inflammatory effects. However, it is not clear whether lycopene supplementation modulates adipokine levelsin vivoin obesity. To determine whether lycopene supplementation can regulate adipokine expression in obesity, male Wistar rats were randomly assigned to receive a control diet (C,n6) or a hyperenergetic diet (DIO,n12) for 6 weeks. After this period, the DIO animals were randomised into two groups: DIO (n6) and DIO supplemented with lycopene (DIO+L,n6). The animals received maize oil (C and DIO) or lycopene (DIO+L, 10 mg/kg body weight (BW) per d) by oral administration for a 6-week period. The animals were then killed by decapitation, and blood samples and epididymal adipose tissue were collected for hormonal determination and gene expression evaluation (IL-6, monocyte chemoattractant protein-1 (MCP-1),TNF-α, leptin and resistin). There was no detectable lycopene in the plasma of the C and DIO groups. However, the mean lycopene plasma concentration was 24 nmol in the DIO+L group. Although lycopene supplementation did not affect BW or adiposity, it significantly decreased leptin, resistin andIL-6gene expression in epididymal adipose tissue and plasma concentrations. Also, it significantly reduced the gene expression ofMCP-1in epididymal adipose tissue. Lycopene affects adipokines by reducing leptin, resistin and plasma IL-6 levels. These data suggest that lycopene may be an effective strategy in reducing inflammation in obesity.

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