Evaluation of the potential influence of the disinfection cycle on the efficacy of strain F Mycoplasma gallisepticum vaccine administered by in ovo injection to layer hatching eggs,,

This study was conducted to determine the percentage of hatchability, post-hatch growth performance, immune response of broiler subjected to in- ovo injection of different doses of rosemary oil. A total of 300 eggs of ross broiler strain were obtained.The experiment distributed into five treatment groups: T1, Negative Control T2, positive control (in ovo injection of 0.05 ml distilled water) T3, in ovo injection of 0.05ml of rosemary oil; T4, in ovo injection of 0.075 ml of rosemary oil; T5, in ovo injection of 0.1 ml of rosemary oil.Highest hatchability percentage was shown in 0.1ml rosemary oil -injected hatching eggs. The final weight of birds from 0.075 and 0.1 ml of rosemary oil-injected eggs was significantly (P < 0.05) highest through the experiment. Feed intake and feed conversion ratio were improved with in ovo administration of rosemary oil at 0.075 and 0.1 ml doses. In addition, the level of blood cholesterol and blood glucose were decreased (p<0.05) in birds from rosemary oil-injected eggs. The obtained data showed a positive effect of in-ovo injection of rosemary oil on stimulating bird's immunity.

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In ovo Injection of Competitive Exclusion Culture in Broiler Hatching Eggs

The Journal of Applied Poultry Research ◽

10.1093/japr/6.3.260 ◽

1997 ◽

Vol 6 (3) ◽

pp. 260-266 ◽

Cited By ~ 16

Author(s):

R. Meijerhof ◽

R.M. Hulet

Keyword(s):

Competitive Exclusion ◽

In Ovo ◽

In Ovo Injection ◽

Hatching Eggs

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The effect of in ovo injection of hatching eggs with omega-3 oil on some productive traits of broilers

University of Thi-Qar Journal of agricultural research ◽

10.54174/utjagr.vo10.n2/05/ ◽

2021 ◽

Keyword(s):

Omega 3 ◽

In Ovo ◽

In Ovo Injection ◽

Productive Traits ◽

Hatching Eggs

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Effect of in-ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broiler chicken

Indian Journal of Animal Research ◽

10.18805/ijar.b-3555 ◽

2018 ◽

Author(s):

P. Kanagaraju ◽

S. Rathnapraba

Keyword(s):

Body Weight ◽

Egg White ◽

Production Performance ◽

Feed Consumption ◽

In Ovo ◽

Egg White Protein ◽

Crypt Depth ◽

Significant Difference ◽

In Ovo Injection ◽

Hatching Eggs

A biological trial was carried out to investigate the effect of in ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broilers. 500 hatching eggs with uniform weight (68±1g) were randomly allotted to five treatment groups each with five replicates of 20 eggs each and incubated. The eggs were injected with one of the following nutrient solutions on 18th day of incubation viz. 0.5 ml of 25 % glucose (T3), 0.5 ml of 12% egg white protein (T4), 0.5 ml of 25 % glucose + egg white protein 12% (T5) into the amnion along this100 eggs served as non injected control (T1) and 100 eggs injected with normal saline (T2) which also served as sham control. The hole was sealed and the period of incubation was continued till 21st day. After hatching, 400 chicks were randomized in to five treatments of five replicates each with 16 chicks. Data on hatchability, hatch weight, bi-weekly body weight, weight gain, feed consumption, FCR and villi height, width, crypt depth of duodenum, jejunum and ileum were recorded and analysed statistically for significant difference. Results revealed that in ovo injection of glucose, egg white protein and their combination significantly (P less than 0.05) increased hatchability, chick weight, body weight and livability. However, the feed consumption was significantly (P less than 0.01) higher in glucose, egg white protein and glucose + egg white protein injected birds compared to non injected control and sham groups. The FCR was significantly (P less than 0.05) improved in all in ovo injected groups compared to control and sham. In ovo feeding significantly (P less than 0.05) increased the duodenal, jejuna and ileal villi height, width and crypt depth (P less than 0.01) compared to control birds. In ovo injection of glucose and EWP either alone or in combination improved the productive performance, gut histomorphology and net profit of the broilers.

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Effects of In-Ovo Injection of Manganese on Some Organ Weights and Lengths in Quail Hatching Eggs

Selcuk Journal of Agricultural and Food Sciences ◽

10.15316/sjafs.2018.104 ◽

2018 ◽

Vol 32 (3) ◽

pp. 345-349

Author(s):

Alp Önder Yıldız ◽

Esra Tuğçe Şentürk ◽

Ali Aygün ◽

Osman Olgun

Keyword(s):

In Ovo ◽

Organ Weights ◽

In Ovo Injection ◽

Hatching Eggs

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Impact of In-Ovo Injection of Folic Acid and Glucose on Hatchability and Post-Hatching Performance of Broiler Chicken

World s Veterinary Journal ◽

10.54203/scil.2020.wvj58 ◽

2020 ◽

pp. 481-491

Author(s):

Amal Ahmed Abdel-Halim ◽

Fatma Rasmy Mohamed ◽

Mohamed Abdel-Rahman El-menawey ◽

Hassan Bayoumi Gharib

Keyword(s):

Folic Acid ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Broiler Breeders ◽

In Ovo ◽

In Ovo Injection ◽

Hatching Eggs ◽

The Impact ◽

Glucose Group

The present study was designed to investigate the impact of in-ovo injection of folic acid and glucose on hatching eggs from 55 weeks old broiler breeders. A total number of 900 hatching eggs were collected from Arbor Acres broiler breeders, then, eggs were divided into 6 groups including 1) Negative Control (non-injected, NC), 2) Dry Punch Control (pricked without injecting any solution, DPC), 3) Positive Control (eggs were injected with 0.5 mL normal saline, PC), 4) Folic Acid group (eggs were injected with 0.2 mg/ egg folic acid, FA), 5) Glucose group (eggs were injected with 125 mg/ egg glucose, Glu), and 6) Folic Acid with Glucose group (eggs were injected with 0.2 mg folic acid with 125 mg/ egg glucose, FA+Glu). Each treatment was divided into five replicates of 30 eggs each. Eggs were injected into the albumen under the air sac. After in-ovo injection, the eggs were stored for four days before hatching. After hatching, the chickens were reared in groups according to the treatments. All treatments were divided into 10 replications of 9 chickens in each. In-ovo injection with folic acid decreased the albumen pH significantly to 9.19 after 4 days of injection, while the negative control was 9.43. Hatching quality was severely affected by all in-ovo injection treatments, but no significant differences were found between the treatment groups concerning the hatchability of fertile eggs. Injection treatments had no significant effect on the growth rate or the production number in any of the weeks. Injection of folic acid and (FA+Glu) significantly increased chickens’ body weight at two and four weeks of age. Also, the dressing percentage when using folic acid and (FA+Glu) was significantly increased to 72.1% and 72.5%, respectively, compared to the positive control group (68.3%). In conclusion, our data suggested that in-ovo injection with a mixture of folic acid and glucose (0.2 mg folic acid+ 125 mg/ egg glucose) could be used to enhance carcass characteristics. Further studies should be conducted to find the effects of in-ovo injection folic acid and glucose on different incubation days and at different sites of injection.

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Effects of in ovo injection of amino acids on hatching performance, cell-mediated immunity and blood profile of FUNAAB Alpha broiler chickens

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i4.998 ◽

2020 ◽

Vol 46 (4) ◽

pp. 59-72

Author(s):

O. M. Sogunle ◽

A. J. Olutayo ◽

O. J. Odutayo ◽

O. A. Oso ◽

O. P. Sobukola ◽

...

Keyword(s):

Amino Acids ◽

Immune Response ◽

Conversion Ratio ◽

Feed Conversion Ratio ◽

Feed Conversion ◽

In Ovo ◽

Blood Profile ◽

Cell Mediated Immune Response ◽

In Ovo Injection ◽

Hatching Eggs

In the study, the effects of in ovo injection of amino acids (L-methionine, L-lysine, and Larginine) and their combinations on hatching traits, post-hatch performance, cell-mediated immune response and blood profile of FUNAAB Alpha broiler chicken were evaluated. The study was carried out in two trials. In the first trial, a total of 360 hatching eggs of indigenous chicken were fumigated, weighed and placed in the incubator. On day 14 of incubation, candling was done and eggs with living embryos were distributed into four treatments; Control (un-injected eggs), L-methionine injected eggs, L-arginine injected eggs and L-lysine injected eggs. Each egg was injected 0.5 mL solution of the amino acid on day18. Hatched chicks were distributed into three replicates containing 30 chicks each. In trial 2, another 360 hatching eggs were used in the treatments which were; Control (eggs without amino acid injection), L-arginine injected eggs, combination of L-arginine and L-methionine injected eggs and combination of L-arginine and L-lysine injected eggs. On day 21 post-hatch, cell-mediated immune response, haematological and serum biochemical parameters were determined. Data obtained from the two trials were subjected to Completely Randomized Design. Results revealed the highest hatchability of 70.27% in the control treatment (un-injected), followed by 51.35% in arginine-injected eggs with the lowest (2.70%) in methionine-injected eggs. Arginine was found to enhance hatchability while L-methionine injected in ovo decreased hatchability. In the second trial, the results showed highest hatchability (89.58%) in eggs under the control, followed by 39.29%, 30.80% and 21.43% in eggs injected with the combination of arginine and lysine, arginine injected eggs, and those with arginine and methionine combination, respectively. Significant (p<0.05) differences in growth performance were only observed in the feed conversion ratio and percentage survivability. Better feed conversion ratio of 2.18 and 2.29 were recorded in birds injected with arginine and the control as against those subjected to combined arginine and methionine (2.96) as well as combined arginine and lysine (3.12). Survivability of 100% was recorded in chickens from arginine-injected eggs in ovo injection and chickens from eggs injected with the combination of arginine and methionine. The study concluded that in ovo injection of arginine either singly or in combination with lysine or methionine positively influenced hatchability, chick weight and growth performance without any deleterious effect on the blood profile of the post-hatch chicks.

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Effects of in ovo injection of Astragalus kahericus polysaccharide on early growth, carcass weights and blood metabolites in broiler chickens

Animal Biotechnology ◽

10.1080/10495398.2021.1924763 ◽

2021 ◽

pp. 1-7

Author(s):

Hussin H. El-Fakhrany ◽

Zenat A. Ibrahim ◽

Elwy A. Ashour ◽

Ali Osman ◽

Mahmoud Alagawany

Keyword(s):

Broiler Chickens ◽

Early Growth ◽

Blood Metabolites ◽

In Ovo ◽

In Ovo Injection

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In Ovo Injection of GABA Can Help Body Weight Gain at Hatch, Increase Chick Weight to Egg Weight Ratio, and Improve Broiler Heat Resistance

Animals ◽

10.3390/ani11051364 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1364

Author(s):

Chris Major Ncho ◽

Akshat Goel ◽

Chae-Mi Jeong ◽

Mohamed Youssouf ◽

Yang-Ho Choi

Keyword(s):

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antioxidant Status ◽

Body Weight Gain ◽

Weight Ratio ◽

The Other ◽

Mrna Levels ◽

In Ovo ◽

Total Antioxidant ◽

In Ovo Injection

The aim of this study was to explore the outcomes of an in ovo GABA injection in broilers challenged with HS. In Experiment 1, 210 Arbor Acres eggs were allocated to five treatments: no-injection, and in ovo injection of 0.6 mL of 0%, 5%, 10%, or 20% of GABA. Hatchling weight and CWEWR were significantly increased in the 5% GABA group. In ovo, injection of 10% GABA solution caused a significant decrease in plasma cholesterol and increased plasma total antioxidant capacity of hatchlings. Experiment 2 was conducted with 126 fertile Arbor Acres eggs distributed into one of two groups. At 17.5 days of incubation, one received no injection, and the other was fed 0.6 mL of 10% GABA. On day 10, one subgroup (4 replicates * 3 birds) from each treatment was submitted to HS (38 ± 1 °C for 3 h) while the other was kept at a thermoneutral temperature (29 ± 1 °C). An in ovo injection of GABA significantly increased total antioxidant capacity, but reduced malondialdehyde levels, hepatic mRNA levels of HSP70, FAS, and L-FABP with HS. In conclusion, an in ovo GABA injection improves CWEWR and antioxidant status at hatch, and enhances antioxidant status while downregulating the expression of HSP70 and fatty acid metabolism-related genes in young chicks under HS.

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