scholarly journals RESPONSE OF BROILER CHICKEN TO INOVO ADMINISTRATION OF DIFFERENT LEVELS OF ROSEMARY OIL(ROSMARINUS OFFICINALIS)

2021 ◽  
Vol 52 (4) ◽  
pp. 896-903
Author(s):  
Sulaiman & Tayeb

This study was conducted to determine the percentage of hatchability, post-hatch growth performance, immune response of broiler  subjected  to in- ovo injection of different doses of rosemary oil. A total of 300  eggs of ross broiler strain were obtained.The experiment distributed into five treatment groups: T1, Negative Control T2, positive control (in ovo injection of 0.05 ml distilled water) T3, in ovo injection of 0.05ml of rosemary oil; T4, in ovo injection of  0.075 ml of rosemary oil; T5, in ovo injection of  0.1 ml of rosemary oil.Highest hatchability  percentage was shown in 0.1ml rosemary oil -injected hatching eggs. The final weight of birds from 0.075 and 0.1 ml of rosemary oil-injected eggs was significantly (P < 0.05) highest through the experiment. Feed intake and feed conversion ratio were improved with in ovo administration of rosemary oil at 0.075 and 0.1 ml doses. In addition, the level of  blood cholesterol and blood glucose  were decreased (p<0.05) in birds from rosemary oil-injected eggs.  The obtained data showed a positive effect of in-ovo injection of rosemary  oil on stimulating bird's immunity.

2020 ◽  
pp. 481-491
Author(s):  
Amal Ahmed Abdel-Halim ◽  
Fatma Rasmy Mohamed ◽  
Mohamed Abdel-Rahman El-menawey ◽  
Hassan Bayoumi Gharib

The present study was designed to investigate the impact of in-ovo injection of folic acid and glucose on hatching eggs from 55 weeks old broiler breeders. A total number of 900 hatching eggs were collected from Arbor Acres broiler breeders, then, eggs were divided into 6 groups including 1) Negative Control (non-injected, NC), 2) Dry Punch Control (pricked without injecting any solution, DPC), 3) Positive Control (eggs were injected with 0.5 mL normal saline, PC), 4) Folic Acid group (eggs were injected with 0.2 mg/ egg folic acid, FA), 5) Glucose group (eggs were injected with 125 mg/ egg glucose, Glu), and 6) Folic Acid with Glucose group (eggs were injected with 0.2 mg folic acid with 125 mg/ egg glucose, FA+Glu). Each treatment was divided into five replicates of 30 eggs each. Eggs were injected into the albumen under the air sac. After in-ovo injection, the eggs were stored for four days before hatching. After hatching, the chickens were reared in groups according to the treatments. All treatments were divided into 10 replications of 9 chickens in each. In-ovo injection with folic acid decreased the albumen pH significantly to 9.19 after 4 days of injection, while the negative control was 9.43. Hatching quality was severely affected by all in-ovo injection treatments, but no significant differences were found between the treatment groups concerning the hatchability of fertile eggs. Injection treatments had no significant effect on the growth rate or the production number in any of the weeks. Injection of folic acid and (FA+Glu) significantly increased chickens’ body weight at two and four weeks of age. Also, the dressing percentage when using folic acid and (FA+Glu) was significantly increased to 72.1% and 72.5%, respectively, compared to the positive control group (68.3%). In conclusion, our data suggested that in-ovo injection with a mixture of folic acid and glucose (0.2 mg folic acid+ 125 mg/ egg glucose) could be used to enhance carcass characteristics. Further studies should be conducted to find the effects of in-ovo injection folic acid and glucose on different incubation days and at different sites of injection.


2019 ◽  
Vol 99 (1) ◽  
pp. 116-122
Author(s):  
Akbar Zamani ◽  
Farid Shariatmadari ◽  
Shaban Rahimi ◽  
Mohammad Amir Karimi Torshizi

A total of 144 ostrich eggs (24 per group) were injected with 4 mL of physiological saline solution [positive control (PC)], carbohydrates (CHO — 20% maltose, 2.5% sucrose, and 2.5% dextrin), β-hydroxy-β-methylbutyrate (HMB), vitamin B6 solution, and vitamin D3 (vit D3) solution at 38 d of incubation. Injection of 4 mL physiological saline decreased the hatchability compared with negative control (NC) group (not injected eggs) (68.5% vs. 71.7%; P < 0.05). There was also lower hatchability and more number of embryonic deaths before piping in vitamin-injected group (72.2%). Heart, gizzard, pancreas, spleen and thymus, ileum, cecum, and rectum relative weight were not significantly different among treatments at hatch (P > 0.05). Carbohydrates group had higher duodenum and jejunum relative weight, whereas NC and PC groups had the least duodenum, jejunum relative weight (P < 0.05). Vit D3 injection increased bone strength, fresh and dry bone relative weight (P < 0.01). Chicks from HMB and CHO group had the highest levels of glycogen in the livers, whereas PC, NC, and vit D3 exhibited very low levels of glycogen in their liver (P = 0.014). Vitamins in ovo injected groups had the least duodenum villus height and also vit D3 group had the least jejunum villus height (P < 0.01). In conclusion, the use of CHO for the in ovo injection of ostrich hatching eggs would be practical in industry due to improvement in hatchability, bone characteristics, small intestine villus height and capacity, and also liver glycogen sources.


Author(s):  
Tülay Çimrin ◽  
Murat Demirel

This experiment was designed to investigate the effects of rosemary essential oils in various doses on hen performance, egg quality, lipid peroxidation malondialdehyde (MDA) level in fresh and stored egg yolk. The experiment was carried out in random parcel experimental design. In the experiment, 6 groups were formed and each group was divided into 5 replicates. In total 240 Bovans hite laying hens, each replicate included 8 hens, 32 weeks old, were used. Treatment groups were control (Negative Control), 500 mg//kg antibiotic (Positive Control 1), 200 mg/kg vitamin E (Positive Control 2), 100 mg/kg rosemary essential oil, 200 mg/kg rosemary essential oil, 300 mg/kg rosemary essential oil. There was no significant difference in live weight, egg production, egg weight, damaged egg ratio, mortality and egg quality but feed comsumption, feed conversion ratio (FCR) and egg yolk MDA values were affected significantly in between control and treatment groups. At all treatment groups feed comsumption decreased significantly and other groups improved in feed conversion ratio except 500 mg/kg antibiotic and 100 mg/kg rosemary doses groups compared to negative control significantly. 100 mg/kg rosemary essential oil addition during the storage time for 42 and 56 days and 200 mg/kg vitamin E addition for all storage time to ration decreased egg yolk MDA values significantly. As a result, laying birds fed 200 mg/kg vitamin E, and 100 mg/kg of rosemary essential oil additives can use to prevent lipid oxidation.


Author(s):  
Nurlaily Agustini ◽  
Arum Setiawan ◽  
Salni Salni

Pomegranate (Punica granatum L.) will be examined by looking at the ability between pomegranate extracts or fractions, which are more effective in lowering total blood cholesterol and triglyceride levels in mice. This study aims to determine the active pomegranate fraction that can reduce the total cholesterol levels and triglyceride in mice. The research method used was an experimental method with a Completely Randomized Design (CRD) which was divided into 6 treatment groups, and test animals divided into 6 treatment groups namely negative control given aquades, positive control fed a high-fat diet, given simvastatin, given 25 mg/grBB of pomegranate methanol fraction, given 50 mg/grBB of pomegranate methanol fraction, given 100 mg/grBB of pomegranate methanol fraction, each treatment group will be divided into 4 male mice.Based on the results of this study that the active fraction that has an effect in reducing blood cholesterol levels and mice triglycerides is the treatment given 100mg/grBB of pomegranate water methanol fraction.


2017 ◽  
Vol 6 (1) ◽  
pp. 15
Author(s):  
Eva Zulviana ◽  
Nurdin Rahman ◽  
Supriadi Supriadi

This study aimed to identify bioactive compounds of moringa (moringa oleifera) extracts and determine the effective concentration on lowering blood cholesterol levels of mice. The animals used were 15 male mice induced by propylthiouracil and fat food diet. The mice were divided randomly into 5 groups with different treatment groups. The first, the second, and the third treatments were given moringa extracts each with a concentration of 10, 20% and 40%. The fourth treatment group was given food fat diet and propiltiorasil as a positive control, and the fifth treatment was given standard food and distilled water as a negative control. Data were analyzed using a statistical analysis of variance (ANOVA) test followed by Duncan test. The results showed that moringa fruit extract contains alkaloids, saponins, flavonoids, and tannins. Moringa fruit extract can lower blood cholesterol levels of mice with the most effective concentration of 10%.


Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 121 ◽  
Author(s):  
Zuzanna Wiśniewska ◽  
Lode Nollet ◽  
Anouk Lanckriet ◽  
Erik Vanderbeke ◽  
Spas Petkov ◽  
...  

The objective of this study was to assess the effect of novel appAT1 and appAT2 phytase inclusion at 250 phytase units (FTU)/kg on weaned piglet performance, the apparent total tract digestibility of P and Ca, and bone mineralization. Piglets (48 males) were randomly divided into four treatment groups: a positive control (PC), with recommended levels of phosphorus (P) and calcium (Ca), a negative control (NC) deficient in P and Ca, and two experimental groups with NC diets supplemented with phytase derived from the appA gene of Escherichia coli. Diets fed in a mashed form were divided into prestarter (0–21 days) and starter (22–42 days) periods. During the whole period of the study, the experimental diets improved (p < 0.05) the body weight gain (BWG) and feed conversion ratio (FCR) compared to the NC diet. The apparent total tract digestibility (ATTD) of the dry matter and crude protein was not significantly different among the diets. Phytase-supplemented diets improved the ATTD of P (p < 0.05) and the ATTD of Ca (p < 0.05). Bone ash content in the third metacarpal and P and Ca content were improved among the phytase supplemented diets compared to the NC diet.


2020 ◽  
Vol 9 (4) ◽  
pp. 233
Author(s):  
Kurdman Sulaiman ◽  
Ihsan T. Tayeb

This study was performed to determine the percentage of hatchability, post-hatch growth performance, and immune response of broiler subjected to in-ovo injection of some natural oils. A total of 360 eggs of ross broiler strain were obtained. Eggs were distributed into six groups: T1,Negative Control (No injection), T2, positive control (injected with 0.1 ml distilled water; T3 injected with 0.1ml of rosemary oil ; T4, injected with 0.1ml of black seed oil; T5 injected with 0.1 ml of olive oil, and T6, injected with 0.1 ml of almond oil. The newly hatched chicks were distributed into 6 groups of 4 replicates of 10 chick. Higher hatchability percentage was found in rosemary oil, followed by almond oil and olive oil –injected eggs. The final weights of birds that were hatched from rosemary oil, and black seed oil -injected groups were significantly (P < 0.05) higher than other experimental groups. Feed intake and feed conversion ratio were improved with in -ovo administration of rosemary oil and black seed oil. In addition, the level of blood cholesterol and blood glucose were decreased (p<0.05) in birds from all-natural oils-injected eggs. The level of IgM and IgG was significantly (p<0.05) increased in the serum of birds that were hatched from natural oils injected eggs. It could be concluded that in-ove injection of natural oils used in this study was promising to increase the hatchability percentage and improve the subsequent growth and immune responses of post hatched birds.


2020 ◽  
Vol 46 (4) ◽  
pp. 59-72
Author(s):  
O. M. Sogunle ◽  
A. J. Olutayo ◽  
O. J. Odutayo ◽  
O. A. Oso ◽  
O. P. Sobukola ◽  
...  

In the study, the effects of in ovo injection of amino acids (L-methionine, L-lysine, and Larginine) and their combinations on hatching traits, post-hatch performance, cell-mediated immune response and blood profile of FUNAAB Alpha broiler chicken were evaluated. The study was carried out in two trials. In the first trial, a total of 360 hatching eggs of indigenous chicken were fumigated, weighed and placed in the incubator. On day 14 of incubation, candling was done and eggs with living embryos were distributed into four treatments; Control (un-injected eggs), L-methionine injected eggs, L-arginine injected eggs and L-lysine injected eggs. Each egg was injected 0.5 mL solution of the amino acid on day18. Hatched chicks were distributed into three replicates containing 30 chicks each. In trial 2, another 360 hatching eggs were used in the treatments which were; Control (eggs without amino acid injection), L-arginine injected eggs, combination of L-arginine and L-methionine injected eggs and combination of L-arginine and L-lysine injected eggs. On day 21 post-hatch, cell-mediated immune response, haematological and serum biochemical parameters were determined. Data obtained from the two trials were subjected to Completely Randomized Design. Results revealed the highest hatchability of 70.27% in the control treatment (un-injected), followed by 51.35% in arginine-injected eggs with the lowest (2.70%) in methionine-injected eggs. Arginine was found to enhance hatchability while L-methionine injected in ovo decreased hatchability. In the second trial, the results showed highest hatchability (89.58%) in eggs under the control, followed by 39.29%, 30.80% and 21.43% in eggs injected with the combination of arginine and lysine, arginine injected eggs, and those with arginine and methionine combination, respectively. Significant (p<0.05) differences in growth performance were only observed in the feed conversion ratio and percentage survivability. Better feed conversion ratio of 2.18 and 2.29 were recorded in birds injected with arginine and the control as against those subjected to combined arginine and methionine (2.96) as well as combined arginine and lysine (3.12). Survivability of 100% was recorded in chickens from arginine-injected eggs in ovo injection and chickens from eggs injected with the combination of arginine and methionine. The study concluded that in ovo injection of arginine either singly or in combination with lysine or methionine positively influenced hatchability, chick weight and growth performance without any deleterious effect on the blood profile of the post-hatch chicks.


Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 678
Author(s):  
Julie C. Lynegaard ◽  
Niels J. Kjeldsen ◽  
Julie K. Bache ◽  
Nicolai R. Weber ◽  
Christian F. Hansen ◽  
...  

Soy protein concentrate improves nutrient utilization and growth performance compared to soybean meal, and diets with a low crude protein (CP) level decreases diarrhoea. The objectives were to (1) test a low CP diet based on different soy products, and (2) to test a very-low CP diet (15.1%) with amino acids (AA) on diarrhoea and productivity. A total of 5,635 weaned pigs (~28 days), were assigned to five dietary treatments; PC (positive control): Standard CP levels (192, 189, 191 g/kg CP) with 2500 ppm ZnO; NC (negative control): Same as PC without ZnO; SP (Soy protein concentrate): Low CP levels (176, 174, 191 g/kg CP); SB (Soybean meal): Low CP levels (177, 176, 191 g/kg CP); and XLA (X-low CP + AA): Very low CP levels (154, 151, 191 g/kg CP) with AA. The PC and XLA diets reduced diarrhoea by 41 and 61%, respectively, compared to the NC group, while no difference between SB and SP were observed. The XLA diet reduced feed intake and daily gain compared with PC and NC, where SP, SB, and XLA had a poorer feed conversion compared with PC. Conclusively, the SP and SB low-protein diets did not reduce diarrhoea or growth performance, whereas the XLA diet decreased both diarrhoea and performance.


Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Mohammed M. Qaid ◽  
Saud I. Al-Mufarrej ◽  
Mahmoud M. Azzam ◽  
Maged A. Al-Garadi ◽  
Hani H. Albaadani ◽  
...  

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.


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