scholarly journals Oral fluid: Non-invasive alternative for parvovirus B19 diagnosis?

2019 ◽  
Vol 117 ◽  
pp. 5-10 ◽  
Author(s):  
Rogier Bodewes ◽  
Jeroen Kerkhof ◽  
Jeroen Cremer ◽  
Daphne B. Gijselaar ◽  
Bettie C.G. Voordouw ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Parvovirus B19 ◽  
Non Invasive

INFLUENCE OF OFFICE BLEACHING SYSTEMS ON THE RESISTANCE OF HARD TOOTH TISSUES

2020 ◽  
pp. 119-125
Author(s):  
Magsumova O.A. ◽  
Postnikov M.A. ◽  
Ryskina E.A. ◽  
Tkach T.M. ◽  
Polkanova V.A.
Keyword(s):  
Informed Consent ◽  
Oral Fluid ◽  
Personal Data ◽  
Acid Resistance ◽  
Non Invasive ◽  
Hard Tissues ◽  
Voluntary Informed Consent

One of the non-invasive methods for treating discoloration of hard tooth tissues is teeth whitening. The aim of this work is to assess the dynamics of changes in the acid resistance of enamel and hard tissues of teeth and the rate of its remineralization after the procedure of office teeth whitening. The study involved 123 patients aged 18 to 35 years with discoloration of various origins, with the color of hard tooth tissues on the Vita Classic A2 scale and darker. Before performing the office, teeth whitening procedure, all patients gave their written voluntary informed consent to participate in this study, as well as consent to the processing of personal data. Depending on the chosen method of office teeth whitening, patients were divided into 3 groups. The resistance of hard tooth tissues was judged based on the determination of TOER and CASRE tests. These indicators were determined at various times (5 days before the office teeth whitening procedure, 5 days after it, after 14, 30 days and 6 months). Regardless of the chosen whitening system, the office teeth whitening procedure is accompanied by a decrease in the enamel's resistance to acids and a decrease in the rate of its remineralization. The remineralizing function of oral fluid promotes the positive dynamics of the studied parameters after 14 days and after 30 days values increased due to the appointment of remineralizing therapy to all patients in 2 weeks after the teeth whitening procedure. After 6 months, all patients had high enamel resistance and the rate of its remineralization.

SALIVA AS HIGHLY INFORMATIVE SUBSTRATE FOR NON-INVASIVE ANALYSIS OF LIPOPEROXIDE PROCESSES AND MUSCLE DAMAGE IN HIQHLY SKILLED ATHLETES

2019 ◽  
Vol 64 (7) ◽  
pp. 405-408 ◽  
Author(s):  
A. N. Ovchinnikov ◽  
A. V. Deryugina
Keyword(s):  
Lipid Peroxidation ◽  
Creatine Kinase ◽  
Muscle Damage ◽  
Oral Fluid ◽  
Track And Field ◽  
Highly Qualified ◽  
Lipid Peroxidation Products ◽  
Non Invasive ◽  
Markers Of Oxidative Stress ◽  
Excessive Accumulation

The purpose of the investigation was to study the efficiency of measuring markers of oxidative stress and muscle damage in the oral fluid in highly skilled sportsmen under physical exercise for the assessment of their functional state. 70 highly qualified athletes at the age of 16-20 years specializing in the cyclic kinds of sports (track and field, swimming) took part in the investigation. Sportsmen performed the control test which consisted of the series of 3×100 m distances by a flat race with an active 45 s rest between them for the track and field athletes, and 4×50 m by the main swimming style with an active rest between the distances also for 45 s for the swimmers. Activity of creatine kinase, content of lipid peroxidation products in the blood and oral fluid were measured standard biochemical methods. The performance of the functional tests induces the excessive accumulation of toxic products of lipoperoxidation and increases activity of creatine kinase in the oral fluid of highly qualified athletes. Correlation analysis shows, that the intensity of free radical and peroxide processes in athletes can be evaluated by means of the method of measuring the content of lipid peroxidation products in the oral fluid.

scholarly journals Pilot Investigation of Anti-Salmonella Antibodies in Oral Fluids from Salmonella Typhimurium Vaccinated and Unvaccinated Swine Herds

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2408
Author(s):  
Alessia De Lucia ◽  
Shaun A. Cawthraw ◽  
Richard Piers Smith ◽  
Rob Davies ◽  
Carlo Bianco ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Oral Fluid ◽  
Igg Antibodies ◽  
Live Attenuated Vaccine ◽  
Non Invasive ◽  
Meat Juice ◽  
Oral Fluids ◽  
Faecal Samples ◽  
Pilot Investigation ◽  
Swine Herds

Oral fluid (OF) can be a simple, cheap and non-invasive alternative to serum or meat juice for the diagnosis and surveillance of important pathogens in pigs. This study was conducted on four Salmonella Typhimurium-positive farrow-to-finish pig farms: two Salmonella-vaccinated (V) and two non-vaccinated (NV). Gilts and sows in the V farms were vaccinated with a live, attenuated vaccine prior to farrowing. Pooled faecal and OF samples were collected from the sows and their offspring. Salmonella was isolated according to ISO6579–1:2017. In parallel, IgG and IgA levels were assessed in OF samples using a commercial ELISA assay. Salmonella was detected in 90.9% of the pooled faecal samples from the NV farms and in 35.1% of the pooled faecal samples from the V farms. Overall, a higher prevalence was observed in the pooled faecal samples from the offspring (76.3%) compared to the sows (36.4%). IgG antibodies measured in V farms are likely to be related to vaccination, as well as exposure to Salmonella field strains. The detection of IgA antibodies in OF was unreliable with the method used. The results of this study show that IgG is the most reliable isotype for monitoring Salmonella-specific antibody immunity in vaccinated/infected animals via OF.

scholarly journals Salivary biomarkers and proteomics: future diagnostic and clinical utilities

2021 ◽  
Vol 19 (3) ◽  
pp. 171-174
Author(s):  
A. V. Mitronin ◽  
O. A. Khvorostenko ◽  
D. A. Ostanina ◽  
Yu. A. Mitronin
Keyword(s):  
Biochemical Parameters ◽  
Oral Fluid ◽  
Biological Fluids ◽  
Inorganic Compounds ◽  
Non Invasive ◽  
Fluid Analysis ◽  
Methods Of Analysis ◽  
Wide Range ◽  
Biological Environment ◽  
Physiological And Biochemical

The search for new, fast and non-invasive methods of diagnosing diseases of both the oral cavity and general diseases of various etiologies and their introduction into practical health care is still a priority in the field of medicine. Among the known methods of analysis of biological fluids, a special place is occupied by the study of saliva. Oral fluid analysis has a high potential in screening for various diseases, since it contains a wide range of organic and inorganic compounds. A significant number of works have been devoted to the study of the quantitative and qualitative composition of the oral fluid, as well as to the study of saliva biomarkers, however, the study of the saliva proteome is at the stage of data accumulation. The lack of standardization in the collection of samples and methods of analysis, as well as poorly studied physiological and biochemical parameters of the oral fluid, hinders the introduction of advances in the study of the saliva proteome into diagnostic practice. The solution of these problems will allow the oral fluid to be used as a biological environment for both detecting diseases and predicting their course.

scholarly journals Adjustments for oral fluid quality and collection methods improve prediction of circulating tetanus antitoxin: Approaches for correcting antibody concentrations detected in a non-invasive specimen

Vaccine ◽  
2021 ◽  
Vol 39 (2) ◽  
pp. 423-430
Author(s):  
Henri Garrison-Desany ◽  
Benard Omondi Ochieng ◽  
Maurice R. Odiere ◽  
Helen Kuo ◽  
Dustin G. Gibson ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Tetanus Antitoxin ◽  
Non Invasive ◽  
Collection Methods

Oral fluid as a diagnostic tool

2004 ◽  
Vol 42 (11) ◽  
Author(s):  
Robin E. Choo ◽  
Marilyn A. Huestis
Keyword(s):  
Diagnostic Tool ◽  
Illicit Drugs ◽  
Oral Fluid ◽  
Illicit Drug ◽  
Therapeutic Drug ◽  
Clinical Tool ◽  
Non Invasive ◽  
The Past ◽  
Technological Advances ◽  
The Relationship

AbstractTechnological advances over the past decades have enabled oral fluid to expand its usefulness in the diagnosis of disease, prediction of disease progression, monitoring of therapeutic drug levels and detection of illicit drugs. The easy non-invasive nature of collection and the relationship between oral fluid and plasma levels make oral fluid a valuable clinical tool. This review describes advances over the past 5 years in the area of oral fluid as a diagnostic tool, its use in therapeutic and illicit drug monitoring, including proposed guidelines for cut-off values, and methods of collection.

Complex Treatment and Prosthetic Rehabilitation of an Acquired Maxillofacial Defect with Tumor Invasion: Case Report

2021 ◽  
Vol 27 ◽  
Author(s):  
Li Jiajing ◽  
Ekaterina V. Kochurova ◽  
Narasimha M Beeraka ◽  
Natalya V. Lapina ◽  
Chang Weilong ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Treatment Plan ◽  
Maxillofacial Surgery ◽  
Complex Treatment ◽  
Tumor Patient ◽  
Non Invasive ◽  
Post Surgery ◽  
Immunological Tests ◽  
Dental Rehabilitation

Background: A patient was evaluated with respect to the effects and results of a complex treatment plan for a complete dental rehabilitation. Several steps were required. Each step included immunological tests of salivary biomarkers. Clinical and immunological assessments were evaluated on Day 3, Week 2, Month 3, and Month 6 post-surgery. These evaluations guided the decision-making process with regard to preparation of a permanent prosthesis. Objective: The aim of the study is to evaluate the response of tissues and organs of the maxillofacial region in patients during dental rehabilitation after maxillofacial surgery. Method: Complex treatment and rehabilitation involving cooperation between the specialists in maxillofacial surgery, prosthetic dentistry, and cancer immunology. Results: Long-term monitoring and clinical examination showed a direct relationship between the patient’s clinical and dental status and the changes in oral fluid biomarkers. Conclusion: The data revealed that the oral fluid biomarkers reflected the patient’s adaptation to prosthodontic rehabilitation. Treatment and monitoring of a maxillofacial tumor patient could use biomarkers as a non-invasive indicator.

scholarly journals aMMP-8 Point-of-Care/Chairside Oral Fluid Technology as a Rapid, Non-Invasive Tool for Periodontitis and Peri-Implantitis Screening in a Medical Care Setting

Diagnostics ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 562 ◽  
Author(s):  
Hanna Lähteenmäki ◽  
Kehinde A. Umeizudike ◽  
Anna Maria Heikkinen ◽  
Ismo T. Räisänen ◽  
Nilminie Rathnayake ◽  
...  
Keyword(s):  
Medical Care ◽  
Oral Fluid ◽  
Care Setting ◽  
Point Of Care ◽  
Active Matrix ◽  
Non Invasive ◽  
Point Of Care Test ◽  
Essential Value ◽  
Periodontal Breakdown ◽  
Periodontal Diagnostics

This communication article addresses currently available rapid non-invasive methods to screen and detect periodontitis and dental peri-implantitis. In this regard, oral fluid biomarkers have been researched extensively but self-reported oral health (SROH)-questionnaires have also been developed. Both alternatives may offer a quick and easy way to screen and detect diseased patients. Active matrix metalloproteinase (aMMP-8) is one of the most validated biomarkers for screening and detecting periodontal breakdown related to periodontitis and peri-implantitis and monitoring their treatment effects revealing successful, less- and non-successful treatment results. Currently available aMMP-8 lateral-flow technologies allow this kind of analysis, as demonstrated here, to be conducted quantitatively online and real-time as point-of-care/chairside testing in dental and even medical care settings. In this study, an aMMP-8 peri-implant sulcular fluid point-of-care-test diagnosed peri-implantitis and healthy implants far more accurately than bleeding-on-probing or the other biomarkers, such as polymorphonuclear (PMN)/neutrophil elastase, myeloperoxidase and MMP-9. Although, SROH-questionnaires allow screening in similar settings but they lack the information about the current disease activity of periodontitis and peri-implantitis, which is of essential value in periodontal diagnostics and treatment monitoring. Thus, both methods can be considered as adjunct methods for periodontitis and peri-implant diagnostics, but the value of oral fluid biomarkers analysis does not seem to be substitutable.

scholarly journals Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar

Pathogens ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 242
Author(s):  
Jose A. Barasona ◽  
Sandra Barroso-Arévalo ◽  
Belén Rivera ◽  
Christian Gortázar ◽  
Jose M. Sánchez-Vizcaíno
Keyword(s):  
Wild Boar ◽  
Mycobacterium Bovis ◽  
Disease Surveillance ◽  
Oral Fluid ◽  
Elisa Test ◽  
Main Concern ◽  
Monitoring And Control ◽  
Non Invasive ◽  
Paired Serum ◽  
And Control

The presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two TB-status areas from Spain and quantitatively used to validate the new ELISA test. Antibodies against MTC were widely detected in OF samples, for which a significant positive correlation (r = 0.83) was found with the validated serology test. OF ELISA sensitivity and specificity were 67.3% and 100%, respectively. The results of this work suggest that OF samples have the potential to be used for MTC diagnosis as a further step in TB surveillance and control in suid populations. Based on our results, further research is warranted and could be performed using non-invasive new tools directly in field conditions to detect exposure to MTC.

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