Adjustments for oral fluid quality and collection methods improve prediction of circulating tetanus antitoxin: Approaches for correcting antibody concentrations detected in a non-invasive specimen

One of the non-invasive methods for treating discoloration of hard tooth tissues is teeth whitening. The aim of this work is to assess the dynamics of changes in the acid resistance of enamel and hard tissues of teeth and the rate of its remineralization after the procedure of office teeth whitening. The study involved 123 patients aged 18 to 35 years with discoloration of various origins, with the color of hard tooth tissues on the Vita Classic A2 scale and darker. Before performing the office, teeth whitening procedure, all patients gave their written voluntary informed consent to participate in this study, as well as consent to the processing of personal data. Depending on the chosen method of office teeth whitening, patients were divided into 3 groups. The resistance of hard tooth tissues was judged based on the determination of TOER and CASRE tests. These indicators were determined at various times (5 days before the office teeth whitening procedure, 5 days after it, after 14, 30 days and 6 months). Regardless of the chosen whitening system, the office teeth whitening procedure is accompanied by a decrease in the enamel's resistance to acids and a decrease in the rate of its remineralization. The remineralizing function of oral fluid promotes the positive dynamics of the studied parameters after 14 days and after 30 days values increased due to the appointment of remineralizing therapy to all patients in 2 weeks after the teeth whitening procedure. After 6 months, all patients had high enamel resistance and the rate of its remineralization.

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SALIVA AS HIGHLY INFORMATIVE SUBSTRATE FOR NON-INVASIVE ANALYSIS OF LIPOPEROXIDE PROCESSES AND MUSCLE DAMAGE IN HIQHLY SKILLED ATHLETES

Russian Clinical Laboratory Diagnostics ◽

10.18821/0869-2084-2019-64-7-405-408 ◽

2019 ◽

Vol 64 (7) ◽

pp. 405-408 ◽

Cited By ~ 1

Author(s):

A. N. Ovchinnikov ◽

A. V. Deryugina

Keyword(s):

Lipid Peroxidation ◽

Creatine Kinase ◽

Muscle Damage ◽

Oral Fluid ◽

Track And Field ◽

Highly Qualified ◽

Lipid Peroxidation Products ◽

Non Invasive ◽

Markers Of Oxidative Stress ◽

Excessive Accumulation

The purpose of the investigation was to study the efficiency of measuring markers of oxidative stress and muscle damage in the oral fluid in highly skilled sportsmen under physical exercise for the assessment of their functional state. 70 highly qualified athletes at the age of 16-20 years specializing in the cyclic kinds of sports (track and field, swimming) took part in the investigation. Sportsmen performed the control test which consisted of the series of 3×100 m distances by a flat race with an active 45 s rest between them for the track and field athletes, and 4×50 m by the main swimming style with an active rest between the distances also for 45 s for the swimmers. Activity of creatine kinase, content of lipid peroxidation products in the blood and oral fluid were measured standard biochemical methods. The performance of the functional tests induces the excessive accumulation of toxic products of lipoperoxidation and increases activity of creatine kinase in the oral fluid of highly qualified athletes. Correlation analysis shows, that the intensity of free radical and peroxide processes in athletes can be evaluated by means of the method of measuring the content of lipid peroxidation products in the oral fluid.

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Pilot Investigation of Anti-Salmonella Antibodies in Oral Fluids from Salmonella Typhimurium Vaccinated and Unvaccinated Swine Herds

Animals ◽

10.3390/ani11082408 ◽

2021 ◽

Vol 11 (8) ◽

pp. 2408

Author(s):

Alessia De Lucia ◽

Shaun A. Cawthraw ◽

Richard Piers Smith ◽

Rob Davies ◽

Carlo Bianco ◽

...

Keyword(s):

Salmonella Typhimurium ◽

Oral Fluid ◽

Igg Antibodies ◽

Live Attenuated Vaccine ◽

Non Invasive ◽

Meat Juice ◽

Oral Fluids ◽

Faecal Samples ◽

Pilot Investigation ◽

Swine Herds

Oral fluid (OF) can be a simple, cheap and non-invasive alternative to serum or meat juice for the diagnosis and surveillance of important pathogens in pigs. This study was conducted on four Salmonella Typhimurium-positive farrow-to-finish pig farms: two Salmonella-vaccinated (V) and two non-vaccinated (NV). Gilts and sows in the V farms were vaccinated with a live, attenuated vaccine prior to farrowing. Pooled faecal and OF samples were collected from the sows and their offspring. Salmonella was isolated according to ISO6579–1:2017. In parallel, IgG and IgA levels were assessed in OF samples using a commercial ELISA assay. Salmonella was detected in 90.9% of the pooled faecal samples from the NV farms and in 35.1% of the pooled faecal samples from the V farms. Overall, a higher prevalence was observed in the pooled faecal samples from the offspring (76.3%) compared to the sows (36.4%). IgG antibodies measured in V farms are likely to be related to vaccination, as well as exposure to Salmonella field strains. The detection of IgA antibodies in OF was unreliable with the method used. The results of this study show that IgG is the most reliable isotype for monitoring Salmonella-specific antibody immunity in vaccinated/infected animals via OF.

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Salivary biomarkers and proteomics: future diagnostic and clinical utilities

Эндодонтия Today ◽

10.36377/1683-2981-2021-19-3-171-174 ◽

2021 ◽

Vol 19 (3) ◽

pp. 171-174

Author(s):

A. V. Mitronin ◽

O. A. Khvorostenko ◽

D. A. Ostanina ◽

Yu. A. Mitronin

Keyword(s):

Biochemical Parameters ◽

Oral Fluid ◽

Biological Fluids ◽

Inorganic Compounds ◽

Non Invasive ◽

Fluid Analysis ◽

Methods Of Analysis ◽

Wide Range ◽

Biological Environment ◽

Physiological And Biochemical

The search for new, fast and non-invasive methods of diagnosing diseases of both the oral cavity and general diseases of various etiologies and their introduction into practical health care is still a priority in the field of medicine. Among the known methods of analysis of biological fluids, a special place is occupied by the study of saliva. Oral fluid analysis has a high potential in screening for various diseases, since it contains a wide range of organic and inorganic compounds. A significant number of works have been devoted to the study of the quantitative and qualitative composition of the oral fluid, as well as to the study of saliva biomarkers, however, the study of the saliva proteome is at the stage of data accumulation. The lack of standardization in the collection of samples and methods of analysis, as well as poorly studied physiological and biochemical parameters of the oral fluid, hinders the introduction of advances in the study of the saliva proteome into diagnostic practice. The solution of these problems will allow the oral fluid to be used as a biological environment for both detecting diseases and predicting their course.

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Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus

Oral Diseases ◽

10.1111/odi.12692 ◽

2017 ◽

Vol 23 (8) ◽

pp. 1072-1079 ◽

Cited By ~ 6

Author(s):

MM Portilho ◽

ACF Mendonça ◽

VA Marques ◽

LC Nabuco ◽

CA Villela-Nogueira ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Oral Fluid ◽

Molecular Detection ◽

Fluid Collection ◽

B Virus ◽

Collection Methods

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Oral fluid: Non-invasive alternative for parvovirus B19 diagnosis?

Journal of Clinical Virology ◽

10.1016/j.jcv.2019.05.008 ◽

2019 ◽

Vol 117 ◽

pp. 5-10 ◽

Cited By ~ 1

Author(s):

Rogier Bodewes ◽

Jeroen Kerkhof ◽

Jeroen Cremer ◽

Daphne B. Gijselaar ◽

Bettie C.G. Voordouw ◽

...

Keyword(s):

Oral Fluid ◽

Parvovirus B19 ◽

Non Invasive

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Oral fluid as a diagnostic tool

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2004.248 ◽

2004 ◽

Vol 42 (11) ◽

Cited By ~ 88

Author(s):

Robin E. Choo ◽

Marilyn A. Huestis

Keyword(s):

Diagnostic Tool ◽

Illicit Drugs ◽

Oral Fluid ◽

Illicit Drug ◽

Therapeutic Drug ◽

Clinical Tool ◽

Non Invasive ◽

The Past ◽

Technological Advances ◽

The Relationship

AbstractTechnological advances over the past decades have enabled oral fluid to expand its usefulness in the diagnosis of disease, prediction of disease progression, monitoring of therapeutic drug levels and detection of illicit drugs. The easy non-invasive nature of collection and the relationship between oral fluid and plasma levels make oral fluid a valuable clinical tool. This review describes advances over the past 5 years in the area of oral fluid as a diagnostic tool, its use in therapeutic and illicit drug monitoring, including proposed guidelines for cut-off values, and methods of collection.

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A superhydrophobic cone to facilitate the xenomonitoring of filarial parasites, malaria, and trypanosomes using mosquito excreta/feces

Gates Open Research ◽

10.12688/gatesopenres.12749.2 ◽

2018 ◽

Vol 1 ◽

pp. 7 ◽

Cited By ~ 3

Author(s):

Darren A.N. Cook ◽

Nils Pilotte ◽

Corrado Minetti ◽

Steven A. Williams ◽

Lisa J. Reimer

Keyword(s):

Cost Effective ◽

Time Cost ◽

Human Pathogens ◽

Non Invasive ◽

Large Numbers ◽

Cost Effective Method ◽

Small Tube ◽

Washing Method ◽

Collection Methods ◽

Molecular Xenomonitoring

Background: Molecular xenomonitoring (MX), the testing of insect vectors for the presence of human pathogens, has the potential to provide a non-invasive and cost-effective method for monitoring the prevalence of disease within a community. Current MX methods require the capture and processing of large numbers of mosquitoes, particularly in areas of low endemicity, increasing the time, cost and labour required. Screening the excreta/feces (E/F) released from mosquitoes, rather than whole carcasses, improves the throughput by removing the need to discriminate vector species since non-vectors release ingested pathogens in E/F. It also enables larger numbers of mosquitoes to be processed per pool. However, this new screening approach requires a method of efficiently collecting E/F. Methods: We developed a cone with a superhydrophobic surface to allow for the efficient collection of E/F. Using mosquitoes exposed to either Plasmodium falciparum, Brugia malayi or Trypanosoma brucei brucei, we tested the performance of the superhydrophobic cone alongside two other collection methods. Results: All collection methods enabled the detection of DNA from the three parasites. Using the superhydrophobic cone to deposit E/F into a small tube provided the highest number of positive samples (16 out of 18) and facilitated detection of parasite DNA in E/F from individual mosquitoes. Further tests showed that following a simple washing step, the cone can be reused multiple times, further improving its cost-effectiveness. Conclusions: Incorporating the superhydrophobic cone into mosquito traps or holding containers could provide a simple and efficient method for collecting E/F. Where this is not possible, swabbing the container or using the washing method facilitates the detection of the three parasites used in this study.

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Complex Treatment and Prosthetic Rehabilitation of an Acquired Maxillofacial Defect with Tumor Invasion: Case Report

Current Pharmaceutical Design ◽

10.2174/1381612827666210224141559 ◽

2021 ◽

Vol 27 ◽

Author(s):

Li Jiajing ◽

Ekaterina V. Kochurova ◽

Narasimha M Beeraka ◽

Natalya V. Lapina ◽

Chang Weilong ◽

...

Keyword(s):

Oral Fluid ◽

Treatment Plan ◽

Maxillofacial Surgery ◽

Complex Treatment ◽

Tumor Patient ◽

Non Invasive ◽

Post Surgery ◽

Immunological Tests ◽

Dental Rehabilitation

Background: A patient was evaluated with respect to the effects and results of a complex treatment plan for a complete dental rehabilitation. Several steps were required. Each step included immunological tests of salivary biomarkers. Clinical and immunological assessments were evaluated on Day 3, Week 2, Month 3, and Month 6 post-surgery. These evaluations guided the decision-making process with regard to preparation of a permanent prosthesis. Objective: The aim of the study is to evaluate the response of tissues and organs of the maxillofacial region in patients during dental rehabilitation after maxillofacial surgery. Method: Complex treatment and rehabilitation involving cooperation between the specialists in maxillofacial surgery, prosthetic dentistry, and cancer immunology. Results: Long-term monitoring and clinical examination showed a direct relationship between the patient’s clinical and dental status and the changes in oral fluid biomarkers. Conclusion: The data revealed that the oral fluid biomarkers reflected the patient’s adaptation to prosthodontic rehabilitation. Treatment and monitoring of a maxillofacial tumor patient could use biomarkers as a non-invasive indicator.

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