Anti-TGF-β1 antibody: An effective treatment for lung injury caused by paraquat in the future

2008 ◽  
Vol 70 (3) ◽  
pp. 705 ◽  
Author(s):  
Xiangdong Jian ◽  
Yanjun Ruan ◽  
Guangran Guo ◽  
Yuanchao Zhang
Keyword(s):  
Lung Injury ◽  
Effective Treatment ◽  
The Future ◽  
Tgf Β1

scholarly journals microRNA-145-5p attenuates acute lung injury via targeting ETS2

2021 ◽  
Author(s):  
Liang Qiao ◽  
Rongxia Li ◽  
Shangang Hu ◽  
Yu Liu ◽  
Hongqiang Liu ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Growth Factor ◽  
Lung Injury ◽  
Inflammatory Response ◽  
Transforming Growth Factor ◽  
Recombinant Adenovirus ◽  
Transforming Growth Factor Β1 ◽  
Inflammatory Factors ◽  
Smad Pathway ◽  
Tgf Β1

Abstract Objective Previously, the protective effect of microRNA (miR)-145-5p has been discovered in acute lung injury (ALI). Thus, this study attempts to further discuss the mechanism of miR-145-5p in ALI through the downstream E26 transformation-specific proto-oncogene 2 (ETS2)/transforming growth factor β1 (TGF-β1)/Smad pathway. Methods A lipopolysaccharide (LPS)-induced rat ALI model was established. Recombinant adenovirus miR-145-5p and/or ETS2 overexpression plasmid was administrated into rats. Afterwards, pathological damage in the lung tissue, wet/dry (W/D) ratio, apoptosis and contents of serum inflammatory factors were observed. miR-145-5p, ETS2, TGF-β1, Smad2/3, phosphorylated Smad2/3 levels were measured in rats. Results miR-145-5p was down-regulated, ETS2 was up-regulated and TGF-β1/Smad pathway was activated in LPS-suffered rats. Overexpression of miR-145-5p inactivated the TGF-β1/Smad pathway and attenuated ALI, as reflected by relived pathological damage, and decreased W/D ratio, apoptosis and inflammatory response. Oppositely, loss of miR-145-5p or enhancement of ETS2 worsened ALI and activated the TGF-β1/Smad pathway. Moreover, elevation of ETS2 decreased miR-145-5p-mediated protection against ALI. Conclusion Evidently, miR-145-5p negatively regulates ETS2 expression and inactivates TGF-β1/Smad pathway to ameliorate ALI in rats.

Ventilator-Induced Lung Injury Is Associated with Neutrophil Infiltration, Macrophage Activation, and TGF-β1 mRNA Upregulation in Rat Lungs

2001 ◽  
Vol 92 (2) ◽  
pp. 428-436 ◽  
Author(s):  
Hideaki Imanaka ◽  
Motomu Shimaoka ◽  
Nariaki Matsuura ◽  
Masaji Nishimura ◽  
Noriyuki Ohta ◽  
...  
Keyword(s):  
Lung Injury ◽  
Macrophage Activation ◽  
Neutrophil Infiltration ◽  
Ventilator Induced Lung Injury ◽  
Rat Lungs ◽  
Tgf Β1

Losartan attenuates paraquat-induced pulmonary fibrosis in rats

2014 ◽  
Vol 34 (5) ◽  
pp. 497-505 ◽  
Author(s):  
F Guo ◽  
YB Sun ◽  
L Su ◽  
S Li ◽  
ZF Liu ◽  
...  
Keyword(s):  
Pulmonary Fibrosis ◽  
Lung Injury ◽  
Mrna Expression ◽  
Collagen Type ◽  
Collagen Type I ◽  
Control Group ◽  
Type I ◽  
Expression Levels ◽  
Relative Expression ◽  
Tgf Β1

Paraquat (PQ) is one of the most widely used herbicides in the world and can cause pulmonary fibrosis in the cases with intoxication. Losartan, an angiotensin II type 1 receptor antagonist, has beneficial effects on the treatment of fibrosis. The aim of this study was to examine the effect of losartan on pulmonary fibrosis in PQ-intoxicated rats. Adult male Sprague Dawley rats ( n = 32, 180–220 g) were randomly assigned to four groups: (i) control group; (ii) PQ group; (iii) PQ + losartan 7d group; and (iv) PQ + losartan 14d group. Losartan treatment (intragastrically (i.g.), 10 mg/kg) was performed for 7 and 14 days after a single i.g. dose of 40 mg/kg PQ. All rats were killed on the 16th day, and hematoxylin–eosin and Masson’s trichrome staining were used to examine lung injury and fibrosis. The levels of hydroxyproline and transforming growth factor β1 (TGF-β1), matrix metallopeptidase 9 (Mmp9), and tissue inhibitor of metalloproteinase 1 (TIMP-1) messenger RNA (mRNA) expression and relative expression levels of collagen type I and III were also detected. PQ caused a significant increase in hydroxyproline content, mRNA expression of TGF-β1, Mmp9, and TIMP-1, and relative expression levels of collagen type I and III (  p < 0.05), while losartan significantly decreased the amount of hydroxyproline and downregulated TGF-β1, Mmp9, and TIMP-1 mRNA and collagen type I and III expressions (  p < 0.05). Histological examination of PQ-treated rats showed lung injury and widespread inflammatory cell infiltration in the alveolar space and pulmonary fibrosis, while losartan could markedly reduce such damage and prevent pulmonary fibrosis. The results of this study indicated that losartan could reduce lung damage and prevent pulmonary fibrosis induced by PQ.

scholarly journals Protective Effects of Myrtol Standardized Against Radiation-Induced Lung Injury

2016 ◽  
Vol 38 (2) ◽  
pp. 619-634 ◽  
Author(s):  
De-yun Zhao ◽  
Hong-jin Qu ◽  
Jia-ming Guo ◽  
Hai-nan Zhao ◽  
Yan-yong Yang ◽  
...  
Keyword(s):  
Single Dose ◽  
Lung Injury ◽  
Major Complication ◽  
The Body ◽  
Protective Effects ◽  
Western Blot Assay ◽  
Tnf Α ◽  
Radiation Induced ◽  
The Impact ◽  
Tgf Β1

Background/Aims: As a major complication after thoracic radiotherapy, radiation-induced lung injury (RILI) has great impact on long term quality of life and could result in fatal respiratory insufficiency The present study was aimed to evaluate the effects of Myrtol standardized on RILI, and to investigate the underlying mechanism. Methods: A mouse model of radiation-induced lung injury was generated by using thoracic irradiation with a single dose of 16Gy. Mice were orally administrated with Myrtol (25 mg/kg/day) for 4 weeks after irradiation, while prednisone (5 mg/kg/day) was used as a positive control. After then, the body weight and lung coefficient were calculated. The severity of fibrosis was evaluated by observing pulmonary sections after radiation and collagen content in lung tissues was calculated following the hydroxyproline (HYP) assay. Pathological changes were observed in all the groups by using HE staining and Masson staining. The serum levels of TGF-β1, TNF-α, IL-1β, IL-6, and PGE2 were also measured with an ELISA assay. Western blot assay was used to measure the impact of Myrtol on AKT and its downstream signaling pathway, including MMP-2 and MMP-9. The levels of Vimentin and α-SMA were evaluated with an immunofluorescence assay. Results: Treatment with Myrtol standardized, but not prednisone, reduced lung coefficient and collagen deposition in lung tissues, while attenuated histological damages induced by irradiation. Myrtol standardized also reduced the production of MDA, while increased the level of SOD. It was also observed that Myrtol standardized inhibited TGF-β1 and a series of pro-inflammatory cytokines including TNF-α, IL-1β, IL-6, PGE2. While in prednisone group, even though the early pneumonitis was ameliorated, the collagen disposition remained unchanged in latter times. Immunofluorescence analysis also revealed elevation of vimentin and α-SMA in the alveoli after a single dose of 16Gy. Conclusion: The present results suggest Myrtol standardized as an effective agent for attenuating the lung injury induced by irradiation.

Ayurveda in changing scenario of diabetes management for developing safe and effective treatment choices for the future

2015 ◽  
Vol 12 (2) ◽  
Author(s):  
Subhadip Banerjee ◽  
Parikshit Debnath ◽  
Prasanna N. Rao ◽  
Tapas Brata Tripathy ◽  
Anjan Adhikari ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Effective Treatment ◽  
Diabetes Management ◽  
Treatment Choices ◽  
The Future

AbstractAyurveda described diabetes mellitus (DM) as

scholarly journals Atractylodin Suppresses TGF-β-Mediated Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells and Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice

2021 ◽  
Vol 22 (20) ◽  
pp. 11152
Author(s):  
Kai-Wei Chang ◽  
Xiang Zhang ◽  
Shih-Chao Lin ◽  
Yu-Chao Lin ◽  
Chia-Hsiang Li ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Pulmonary Fibrosis ◽  
Lung Injury ◽  
Epithelial Mesenchymal Transition ◽  
A549 Cells ◽  
Alveolar Epithelial Cells ◽  
Collagen Deposition ◽  
Mesenchymal Transition ◽  
Alveolar Epithelial ◽  
Tgf Β1

Idiopathic pulmonary fibrosis (IPF) is characterized by fibrotic change in alveolar epithelial cells and leads to the irreversible deterioration of pulmonary function. Transforming growth factor-beta 1 (TGF-β1)-induced epithelial-mesenchymal transition (EMT) in type 2 lung epithelial cells contributes to excessive collagen deposition and plays an important role in IPF. Atractylodin (ATL) is a kind of herbal medicine that has been proven to protect intestinal inflammation and attenuate acute lung injury. Our study aimed to determine whether EMT played a crucial role in the pathogenesis of pulmonary fibrosis and whether EMT can be utilized as a therapeutic target by ATL treatment to mitigate IPF. To address this topic, we took two steps to investigate: 1. Utilization of anin vitro EMT model by treating alveolar epithelial cells (A549 cells) with TGF-β1 followed by ATL treatment for elucidating the underlying pathways, including Smad2/3 hyperphosphorylation, mitogen-activated protein kinase (MAPK) pathway overexpression, Snail and Slug upregulation, and loss of E-cadherin. Utilization of an in vivo lung injury model by treating bleomycin on mice followed by ATL treatment to demonstrate the therapeutic effectiveness, such as, less collagen deposition and lower E-cadherin expression. In conclusion, ATL attenuates TGF-β1-induced EMT in A549 cells and bleomycin-induced pulmonary fibrosis in mice.

SarsCo-V2 Induced Organizing Pneumonia: Are We Failing To Initiate Effective Treatment For The Most Common Form Of COVID-19 Lung Injury? (Preprint)

2020 ◽  
Author(s):  
Pierre Kory, MD ◽  
Jeffrey P Kanne
Keyword(s):  
Lung Injury ◽  
Effective Treatment ◽  
Corticosteroid Therapy ◽  
Viral Infections ◽  
Injury Pattern ◽  
Organizing Pneumonia ◽  
Post Mortem ◽  
Common Complication ◽  
Pathologic Findings ◽  
Lung Biopsies

UNSTRUCTURED The persistent harms to COVID-19 patients caused by the under-recognition of a treatable and commonly reported radiographic lung injury pattern in COVID-19 is discussed. Pathologic findings of organizing pneumonia in post-mortem lung biopsies and autopsies are reviewed, concluding that many patients are suffering from a condition called secondary organizing pneumonia, a common complication of certain viral infections, including in the prior coronavirus pandemics. Initiation of immediate corticosteroid therapy in all patients presenting with secondary organizing pneumonia syndromes due to Sars-CoV2 may alter the clinical trajectories of many patients away from ventilators and ICU beds while dramatically reducing the morbidity and mortality of this disease.

Nanomedicine to advance the treatment of bacteria-induced acute lung injury

2021 ◽  
Author(s):  
Ruonan Su ◽  
Yu Zhang ◽  
Jiabin Zhang ◽  
Haixia Wang ◽  
Yun Luo ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Mortality Rate ◽  
Lung Injury ◽  
Supportive Care ◽  
Effective Treatment ◽  
High Mortality

Bacteria-induced acute lung injury (ALI) is associated with a high mortality rate due to the lack of an effective treatment. Patients often rely on supportive care such as low tidal...

Botulinum toxins in the treatment of migraines

2020 ◽  
Vol 9 (3) ◽  
pp. 114-117
Author(s):  
Tariq Sumrein
Keyword(s):  
Botulinum Toxin ◽  
Effective Treatment ◽  
Drug Administration ◽  
Food And Drug Administration ◽  
Botulinum Toxins ◽  
Aesthetic Medicine ◽  
The Us ◽  
The Future

Ten years ago, Botulinum toxin was approved by the US Food and Drug Administration for the treatment of migraines. Dr Tariq Sumrein details how this effective treatment works and what this means for aesthetic practitioners and the future of aesthetic medicine

Sign in / Sign up

Export Citation Format

Share Document