Photoreactive-proton-generating hyaluronidase/albumin nanoparticles-loaded PEG-hydrogel enhances antitumor efficacy and disruption of the hyaluronic acid extracellular matrix in AsPC-1 tumors

As a component of extracellular matrix (ECM), hyaluronic acid (HA) has plenty of applications in biomedical field such as tissue engineering. Due to its non-adhesive nature, HA requires further functional...

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Hyaluronic acid levels are increased in complicated parapneumonic pleural effusions

Monaldi Archives for Chest Disease ◽

10.4081/monaldi.2011.217 ◽

2015 ◽

Vol 75 (3) ◽

Cited By ~ 1

Author(s):

T. Zaga ◽

D. Makris ◽

I. Tsilioni ◽

T. Kiropoulos ◽

S. Oikonomidi ◽

...

Keyword(s):

Heart Failure ◽

Extracellular Matrix ◽

Congestive Heart Failure ◽

Hyaluronic Acid ◽

Pleural Fluid ◽

Inflammatory Process ◽

Serum Levels ◽

Pleural Effusions ◽

Tnf Α ◽

Malignant Effusions

Background and Aim. Hyaluronic acid (HA) is a component of extracellular matrix and may play a role in the pleural inflammation which is implicated in parapneumonic effusions.The aim of the current study was to investigate HA levels in serum and pleura in patients with parapneumonic effusions. Methods. We prospectively studied pleural and serum levels of HA in 58 patients with pleural effusions due to infection (complicated and uncomplicated parapneumonic effusions), malignant effusions and transudative effusions due to congestive heart failure. In addition to HA, TNF-α and IL-1β levels were determined in pleural fluid and serum by ELISA. Results. The median±SD HA levels (pg/ml) in pleural fluid of patients with complicated effusions (39.058±11.208) were significantly increased (p<0.005), compared to those with uncomplicated parapneumonic effusions (11.230±1.969), malignant effusions (10.837±4.803) or congestive heart failure (5.392±3.133). There was no correlation between pleural fluid and serum HA values. Pleural fluid TNF-α levels (146±127 pg/mL) and IL-1β levels (133.4±156 pg/mL) were significantly higher in patients with complicated parapneumonic effusions compared to patients with other types of effusion (p<0.05). No significant association between HA and TNF-α or IL-1β was found. Conclusions. HA may play a significant role in the inflammatory process which characterises exudative infectious pleuritis. Further investigation might reveal whether HA is a useful marker in the management of parapneumonic effusions.

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4-Methylumbelliferone Attenuates Macrophage Invasion and Myocardial Remodeling in Pressure-Overloaded Mouse Hearts

Hypertension ◽

10.1161/hypertensionaha.120.15247 ◽

2021 ◽

Author(s):

Katarzyna Hackert ◽

Susanne Homann ◽

Shakila Mir ◽

Arne Beran ◽

Simone Gorreßen ◽

...

Keyword(s):

Extracellular Matrix ◽

Hyaluronic Acid ◽

Immune Cells ◽

Inflammatory Responses ◽

Pressure Overload ◽

Myocardial Damage ◽

Left Ventricular ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling ◽

Cardiac Wall

Cardiac wall stress induces local and systemic inflammatory responses that are increasingly recognized as key modulators of extracellular matrix remodeling. Hyaluronic acid interacts with immune cells and mesenchymal cells thereby modulating profibrotic signals. Here we tested the hypothesis that 4-methylumbelliferone (4-MU), an inhibitor of hyaluronic acid synthesis, would attenuate inflammation and extracellular matrix remodeling of pressure-overloaded myocardium in C57BL/6J male mice fed with 4-MU and subjected to TAC (transverse aortic constriction) surgery. Flow cytometry of immune cells showed TAC-induced leukocytosis due to an increase of neutrophils and monocytes. 4-MU strongly attenuated both circulating and cardiac leukocyte numbers 3 days after TAC. In the hearts, 4-MU reduced the number of CCR2 − resident macrophages. At later time points, 4-MU also prevented the infiltration of heart tissue by bone marrow-derived circulating monocytes leading to reduced cardiac macrophage counts even 7 weeks after TAC. The long-term attenuation of macrophage-driven inflammation was associated with less myocardial fibrosis in 4-MU-treated compared with untreated mice. Unexpectedly, 4-MU also reduced the development of left ventricular hypertrophy and increased cardiac output after TAC without affecting blood pressure. The data demonstrate that 4-MU reduces both resident and invading cardiac macrophages and may be a promising agent to alleviate pressure-overload induced myocardial damage.

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The extracellular matrix of rat spinal cord: A comparative study on the localization of hyaluronic acid, glial hyaluronate-binding protein, and chondroitin sulfate proteoglycan

Experimental Neurology ◽

10.1016/0014-4886(92)90115-7 ◽

1992 ◽

Vol 117 (1) ◽

pp. 90-93 ◽

Cited By ~ 22

Author(s):

A. Bignami ◽

R. Asher ◽

G. Perides

Keyword(s):

Spinal Cord ◽

Extracellular Matrix ◽

Hyaluronic Acid ◽

Comparative Study ◽

Chondroitin Sulfate ◽

Binding Protein ◽

Chondroitin Sulfate Proteoglycan ◽

Sulfate Proteoglycan ◽

Rat Spinal Cord

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Tau Pathology Promotes the Reorganization of the Extracellular Matrix and Inhibits the Formation of Perineuronal Nets by Regulating the Expression and the Distribution of Hyaluronic Acid Synthases

Journal of Alzheimer s Disease ◽

10.3233/jad-160804 ◽

2017 ◽

Vol 57 (2) ◽

pp. 395-409 ◽

Cited By ~ 13

Author(s):

Yin Li ◽

Ze-Xu Li ◽

Tan Jin ◽

Zhan-You Wang ◽

Pu Zhao

Keyword(s):

Extracellular Matrix ◽

Hyaluronic Acid ◽

Perineuronal Nets ◽

Tau Pathology

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Polycaprolactone/Gelatin/Hyaluronic Acid Electrospun Scaffolds to Mimic Glioblastoma Extracellular Matrix

Materials ◽

10.3390/ma13112661 ◽

2020 ◽

Vol 13 (11) ◽

pp. 2661 ◽

Cited By ~ 2

Author(s):

Semra Unal ◽

Sema Arslan ◽

Betul Karademir Yilmaz ◽

Faik Nuzhet Oktar ◽

Denisa Ficai ◽

...

Keyword(s):

Extracellular Matrix ◽

Hyaluronic Acid ◽

Rotational Velocity ◽

Axon Growth ◽

Scanning Calorimetry ◽

Composite Scaffolds ◽

Water Contact ◽

Human Brain Tumor ◽

Novel Therapeutic Strategies

Glioblastoma (GBM), one of the most malignant types of human brain tumor, is resistant to conventional treatments and is associated with poor survival. Since the 3D extracellular matrix (ECM) of GBM microenvironment plays a significant role on the tumor behavior, the engineering of the ECM will help us to get more information on the tumor behavior and to define novel therapeutic strategies. In this study, polycaprolactone (PCL)/gelatin(Gel)/hyaluronic acid(HA) composite scaffolds with aligned and randomly oriented nanofibers were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor. We investigated the effect of nanotopography and components of fibers on the mechanical, morphological, and hydrophilic properties of electrospun nanofiber as well as their biocompatibility properties. Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) have been used to investigate possible interactions between components. The mean fiber diameter in the nanofiber matrix was increased with the presence of HA at low collector rotation speed. Moreover, the rotational velocity of the collector affected the fiber diameters as well as their homogenous distribution. Water contact angle measurements confirmed that hyaluronic acid-incorporated aligned nanofibers were more hydrophilic than that of random nanofibers. In addition, PCL/Gel/HA nanofibrous scaffold (7.9 MPa) exhibited a significant decrease in tensile strength compared to PCL/Gel nanofibrous mat (19.2 MPa). In-vitro biocompatibilities of nanofiber scaffolds were tested with glioblastoma cells (U251), and the PCL/Gel/HA scaffolds with random nanofiber showed improved cell adhesion and proliferation. On the other hand, PCL/Gel/HA scaffolds with aligned nanofiber were found suitable for enhancing axon growth and elongation supporting intracellular communication. Based on these results, PCL/Gel/HA composite scaffolds are excellent candidates as a biomimetic matrix for GBM and the study of the tumor.

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